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The map label for this gene is mdtO [H]

Identifier: 170681173

GI number: 170681173

Start: 4646635

End: 4648686

Strand: Reverse

Name: mdtO [H]

Synonym: EcSMS35_4545

Alternate gene names: 170681173

Gene position: 4648686-4646635 (Counterclockwise)

Preceding gene: 170681502

Following gene: 170681660

Centisome position: 91.72

GC content: 54.92

Gene sequence:

>2052_bases
ATGAGCGCGCTCAACTCCCTGCCATTACCGGTGGTCAGGCTGCTGGCGTTCTTTCATGAAGAGTTAAGCGAGCGGCGGCC
AGGTCGTGTGCCGCAGACCGTGCAACTCTGGGTAGGCTGCCTGCTGGTGATTCTGATCTCGATGACCTTCGAGATCCCCT
TTGTGGCGTTATCGCTGGCGGTGCTGTTTTACGGTATTCAGTCCAACGCGTTTTACACCAAATTTGTCGCGATCTTGTTT
GTGGTTGCCACGGTGCTGGAGATCGGCAGCCTGTTTTTGATCTACAAATGGTCATACGGCGAACCGTTGATCCGATTGAT
CATCGCAGGGCCGATCCTGATGGGATGTATGTTTTTGATGCGCACCCATCGGCTGGGACTGGTCTTTTTCGCTGTCGCCA
TTGTCGCCATTTACGGGCAAACCTTCCCCGCCATGCTCGACTATCCGGAAGTGGTCGTGCGCTTAACGCTGTGGTGTATC
GTTGTTGGCCTCTATCCAACCTTGCTGATGACGTTAATCGGCGTGCTGTGGTTTCCCAGCCGCGCTATCACGCAGATGCA
TCAGGCGCTTAATGATCGGCTTGATGATGCCATTAGCCACCTGACGAACAGCCTCACACCGCTACCCGAAACGCGGATTG
AAAGAGAGGCGCTGGCGCTGCAAAAACTCAATGTCTTTTGCCTCGCGGACGATGCCGACTGGCGTACTCAGAGCGCATGG
TGGCAAAGCTGTGTGGCAACGGTAACCTACATTTACTCGACGCTGAATCGCTACGATCCCACCTCTTTTGCTGATTCTCA
GGCAATTATTGAATTCCGGCAAAAATTAGCCTCAGAAATCAACAAGCTGCAGCATGCCATTGCCGAAGGTCAGTGCTGGC
AAAGCGACTGGCGGATCACTGAAAGTCAAGCGATGGCGGCACGGGAATGTAACCTGGAGAATATCTGCCAGACATTGTTG
CAACTGGGTCAGATGGACCCGAATACGCCGCCAACGCCCGCCACCAAACCGCCATCAATGGTCGCCGACGCTTTTACCAA
TCCAGACTATATGCGCTACGCGGTAAAAACGCTGCTCGCCTGTTTGATCTGTTACACCTTCTACAGCGGCGTGGACTGGG
AAGGCATTCACACCTGTATGCTGACCTGCGTGATCGTCGCTAATCCAAATGTCGGTTCGTCGTACCAGAAGATGGTGCTG
CGTTTTGGCGGGGCCTTTTGCGGTGCGATTCTGGCGCTGTTATTCACGCTACTGGTCATGCCCTGGCTGGACAATATTGT
CGAATTGCTGTTTGTGCTGGCACCGATTTTCCTGTTGGGCGCATGGATTGCCACCAGCTCTGAACGCTCTTCTTATATCG
GCACACAGATGGTGGTCACCTTCGCGCTCGCCACGCTCGAAAATGTTTTTGGCCCGGTGTACGACCTGGTGGAAATTCGC
GATCGCGCCCTGGGTATCATCATTGGTACCGTGGTGTCCGCGGTGATTTACACCTTTGTCTGGCCTGAAAGTGAAGCGCG
CACCCTGCCGCAAAAACTGGCTGGCGCGCTGGGTATGTTAAGTAAAGTAATGCGGATCCCACGCCAGCAGGAAGTCACGG
CTCTGCGCACTTATCTGCAAATTCGCATAGGTCTGCATGCGGCGTTTAATGCCTGTGAAGAGATGTGCCAACGCGTGGCG
CTGGAGCGTCAACTGGACAGCGAAGAACGCGCCTTACTGATTGAACGTTCGCAAACGGTTATTCGTCAGGGTCGCGATAT
TCTTCACGCCTGGGATGCAACCTGGAACTCGGCGCAGGCGCTGGATAACGCACTACAGCCGGACAGAGCTGGTCAGTTTG
CCGACGCCCTGGAGAAATACGCTGCCGGTCTGGCAACCGCACTCAGTCGTTCTCCTCAAATAACGCTTGAAGAAACACCC
GCCTCGCAGGCCATCCTGCCGACCTTATTAAAACAGGAGCAACACGTCTGCCAGCTTTTCGCCCGCTTGCCAGACTGGAC
AGCCCCGGCATTAACGCCCGCCACGGAACAGGCACAAGGAGCCACGCAATGA

Upstream 100 bases:

>100_bases
GCGTTGCCCAGCGTTTTCCGGTCAAAATCATGGTTGAAAAACCTGACCCGGAAATGTTCCGCATCGGCGCTTCGGCAGTC
GCTAATCTTGAGCCACAATA

Downstream 100 bases:

>100_bases
TCAATCGTCAACTTTCACGCCTGCTGTTGTGCAGCATTCTCGGCAGCACGACGCTGATTTCCGGCTGTGCCCTGGTACGT
AAGGATTCTGCGCCTCATCA

Product: multidrug efflux system protein MdtO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILF
VVATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTNSLTPLPETRIEREALALQKLNVFCLADDADWRTQSAW
WQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAIAEGQCWQSDWRITESQAMAARECNLENICQTLL
QLGQMDPNTPPTPATKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVL
RFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIR
DRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVA
LERQLDSEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATALSRSPQITLEETP
ASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ

Sequences:

>Translated_683_residues
MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILF
VVATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTNSLTPLPETRIEREALALQKLNVFCLADDADWRTQSAW
WQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAIAEGQCWQSDWRITESQAMAARECNLENICQTLL
QLGQMDPNTPPTPATKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVL
RFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIR
DRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVA
LERQLDSEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATALSRSPQITLEETP
ASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ
>Mature_682_residues
SALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFV
VATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCIV
VGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTNSLTPLPETRIEREALALQKLNVFCLADDADWRTQSAWW
QSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAIAEGQCWQSDWRITESQAMAARECNLENICQTLLQ
LGQMDPNTPPTPATKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVLR
FGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIRD
RALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVAL
ERQLDSEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATALSRSPQITLEETPA
SQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ

Specific function: Could be involved in resistance to puromycin, acriflavine and tetraphenylarsonium chloride [H]

COG id: COG1289

COG function: function code S; Predicted membrane protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MdtO family [H]

Homologues:

Organism=Escherichia coli, GI87082367, Length=683, Percent_Identity=98.2430453879941, Blast_Score=1382, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006726 [H]

Pfam domain/function: PF04632 FUSC [H]

EC number: NA

Molecular weight: Translated: 76224; Mature: 76093

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCIVVGLYPTLLMTLIGVLWFPS
HHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RAITQMHQALNDRLDDAISHLTNSLTPLPETRIEREALALQKLNVFCLADDADWRTQSAW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHH
WQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAIAEGQCWQSDWRIT
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESQAMAARECNLENICQTLLQLGQMDPNTPPTPATKPPSMVADAFTNPDYMRYAVKTLLA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
CLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVLRFGGAFCGAILALLFTLLVM
HHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IRIGLHAAFNACEEMCQRVALERQLDSEERALLIERSQTVIRQGRDILHAWDATWNSAQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHH
LDNALQPDRAGQFADALEKYAAGLATALSRSPQITLEETPASQAILPTLLKQEQHVCQLF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
ARLPDWTAPALTPATEQAQGATQ
HHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVILISMTFEIPFVALSLA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEPLIRLIIAGPILMGCMFLM
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
RTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCIVVGLYPTLLMTLIGVLWFPS
HHHHHHHHHHHHHHHHHHHCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RAITQMHQALNDRLDDAISHLTNSLTPLPETRIEREALALQKLNVFCLADDADWRTQSAW
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHH
WQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASEINKLQHAIAEGQCWQSDWRIT
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ESQAMAARECNLENICQTLLQLGQMDPNTPPTPATKPPSMVADAFTNPDYMRYAVKTLLA
HHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHH
CLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSYQKMVLRFGGAFCGAILALLFTLLVM
HHHHHHHHCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWLDNIVELLFVLAPIFLLGAWIATSSERSSYIGTQMVVTFALATLENVFGPVYDLVEIR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRALGIIIGTVVSAVIYTFVWPESEARTLPQKLAGALGMLSKVMRIPRQQEVTALRTYLQ
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IRIGLHAAFNACEEMCQRVALERQLDSEERALLIERSQTVIRQGRDILHAWDATWNSAQA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHH
LDNALQPDRAGQFADALEKYAAGLATALSRSPQITLEETPASQAILPTLLKQEQHVCQLF
HHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHH
ARLPDWTAPALTPATEQAQGATQ
HHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]