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The map label for this gene is ecpD [H]

Identifier: 170680970

GI number: 170680970

Start: 165865

End: 166605

Strand: Reverse

Name: ecpD [H]

Synonym: EcSMS35_0151

Alternate gene names: 170680970

Gene position: 166605-165865 (Counterclockwise)

Preceding gene: 170681382

Following gene: 170679612

Centisome position: 3.29

GC content: 45.88

Gene sequence:

>741_bases
ATGTTTTTAAACGCTAAACATACTGCAGCTGTATGCTTCGCCACTTGTATGAGCTTAAGCGCATCGGCAATAGCAGATAT
CGTAATTTCAGGAACTCGCGTAATTTATAAAAGTAATGAAAAAAGCGTTAATGTTCGGCTGGAAAATAAGGGGAATAATC
CGTTACTCGTTCAGAGCTGGCTGGATACTGGCGACGATAGTGCTGAACCAGGGAGTATTACTGTCCCATTTACCGCCACC
CCTCCTGTTTCGCGAATTGATGCCAAACGTGGTCAAACTATTAAATTGATGTACACGGCTTCCTCACCGCTGCCAAAAGA
CCGAGAAAGTGTTTTCTGGTTTAACGTACTCGAGGTTCCACCCAAACCTGACGCAGATAAAGTCGCCAACCAGAGTCTTC
TGCAATTGGCATTCCGTACGCGTATCAAATTATTTTATCGCCCGGAAGGTTTAAACGGACTGCCATCAGATGCACCCGCA
GCACTGAAATGGTCATGGGCAACCTCTGCTGGAAAAACTGCATTGCGCGTCGCAAATCCGACACCGTATCACGTCTCATT
CAGTAGTGGTGATTTAGTCGCCAATGGCAAAACCTACCCCCTGGATGTAAAAATGGTAGCCCCGCTGAGTGAAGAAACCT
TCGATATAAAAGGCATGAGTGGTAAAGCAAGTTCTGCCAAAGTGCATTTCTTCGCCATAAATGACTTTGGTGGTGCAATT
GAAGGCAATGCCAGCCTGTAA

Upstream 100 bases:

>100_bases
TAATCCTTCGTTAGATTCATAAACTGAATATCTATGGGAGTAACTTTCTTAATAGTGGAATGTTACTCCCTATTTTAATT
AACTCTGTCGGGAATGGTTT

Downstream 100 bases:

>100_bases
CTAAATCAGGAATAAACGCAGCAGGAAGCACAACGTGTCCCGAAAAATCATAAAAACGCATAATAACAAACTTACATATA
TAGCCTCGTTTTGCGCATTG

Product: putative chaperone protein EcpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MFLNAKHTAAVCFATCMSLSASAIADIVISGTRVIYKSNEKSVNVRLENKGNNPLLVQSWLDTGDDSAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASSPLPKDRESVFWFNVLEVPPKPDADKVANQSLLQLAFRTRIKLFYRPEGLNGLPSDAPA
ALKWSWATSAGKTALRVANPTPYHVSFSSGDLVANGKTYPLDVKMVAPLSEETFDIKGMSGKASSAKVHFFAINDFGGAI
EGNASL

Sequences:

>Translated_246_residues
MFLNAKHTAAVCFATCMSLSASAIADIVISGTRVIYKSNEKSVNVRLENKGNNPLLVQSWLDTGDDSAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASSPLPKDRESVFWFNVLEVPPKPDADKVANQSLLQLAFRTRIKLFYRPEGLNGLPSDAPA
ALKWSWATSAGKTALRVANPTPYHVSFSSGDLVANGKTYPLDVKMVAPLSEETFDIKGMSGKASSAKVHFFAINDFGGAI
EGNASL
>Mature_246_residues
MFLNAKHTAAVCFATCMSLSASAIADIVISGTRVIYKSNEKSVNVRLENKGNNPLLVQSWLDTGDDSAEPGSITVPFTAT
PPVSRIDAKRGQTIKLMYTASSPLPKDRESVFWFNVLEVPPKPDADKVANQSLLQLAFRTRIKLFYRPEGLNGLPSDAPA
ALKWSWATSAGKTALRVANPTPYHVSFSSGDLVANGKTYPLDVKMVAPLSEETFDIKGMSGKASSAKVHFFAINDFGGAI
EGNASL

Specific function: Probably mediates assembly of a pilin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=246, Percent_Identity=81.7073170731707, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1788428, Length=228, Percent_Identity=36.8421052631579, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1789532, Length=238, Percent_Identity=34.453781512605, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1790771, Length=231, Percent_Identity=32.4675324675325, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1786743, Length=239, Percent_Identity=31.3807531380753, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788677, Length=190, Percent_Identity=37.3684210526316, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786936, Length=224, Percent_Identity=27.6785714285714, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787171, Length=225, Percent_Identity=30.2222222222222, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI87082203, Length=243, Percent_Identity=31.2757201646091, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI87081806, Length=233, Percent_Identity=31.7596566523605, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 26453; Mature: 26453

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLNAKHTAAVCFATCMSLSASAIADIVISGTRVIYKSNEKSVNVRLENKGNNPLLVQSW
CEECCCHHHHHHHHHHHHCCHHHHHHEEECCEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDSAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASSPLPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECC
PKPDADKVANQSLLQLAFRTRIKLFYRPEGLNGLPSDAPAALKWSWATSAGKTALRVANP
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCC
TPYHVSFSSGDLVANGKTYPLDVKMVAPLSEETFDIKGMSGKASSAKVHFFAINDFGGAI
CCEEEEECCCCEEECCCCCEEEEEEECCCCCCCEEEECCCCCCCCEEEEEEEEECCCCEE
EGNASL
CCCCCC
>Mature Secondary Structure
MFLNAKHTAAVCFATCMSLSASAIADIVISGTRVIYKSNEKSVNVRLENKGNNPLLVQSW
CEECCCHHHHHHHHHHHHCCHHHHHHEEECCEEEEEECCCCEEEEEEECCCCCCEEEEEE
LDTGDDSAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASSPLPKDRESVFWFNVLEVP
CCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEEEEECC
PKPDADKVANQSLLQLAFRTRIKLFYRPEGLNGLPSDAPAALKWSWATSAGKTALRVANP
CCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCC
TPYHVSFSSGDLVANGKTYPLDVKMVAPLSEETFDIKGMSGKASSAKVHFFAINDFGGAI
CCEEEEECCCCEEECCCCCEEEEEEECCCCCCCEEEECCCCCCCCEEEEEEEEECCCCEE
EGNASL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8102362; 8202364; 9278503 [H]