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The map label for this gene is hypF [H]

Identifier: 170680945

GI number: 170680945

Start: 2910351

End: 2912639

Strand: Reverse

Name: hypF [H]

Synonym: EcSMS35_2835

Alternate gene names: 170680945

Gene position: 2912639-2910351 (Counterclockwise)

Preceding gene: 170682992

Following gene: 170679621

Centisome position: 57.47

GC content: 57.97

Gene sequence:

>2289_bases
GTGTCCGGCAGGGCGGGTTACGAAATTCATGATGGCGGGAATAACTCAATGGCAAAAAACATATCTTGCGGTGTCCAACT
GCGTATTCGTGGCAAAGTGCAGGGCGTCGGTTTTCGTCCGTTTGTCTGGCAACTGGCACAGCAATTAAATCTTCACGGCG
ATGTCTGTAATGACGGCGATGGCGTAGAAGTCCGGCTGCTGGAAGCCCCGGAAACGTTTCTTGTTCAATTGCATCAGCAC
TGCCCGCCGCTGGCGCGTATTGATAGCGTCGAGCGTGAGCCGTTTATCTGGTCACAACTGCCCACTGAGTTCACTATCCG
CCAGAGCACAGGCGGCACCATGAATACGCAAATTGTCCCGGATGCCGCCACTTGCCCTGCTTGCCTTGCCGAAATGAATA
CCCCGGGCGAGCGGCGTTATCGTTATCCGTTTATCAACTGTACCCACTGCGGCCCGCGTTTCACCATTATTCGCGCGATG
CCTTACGACCGCCCGTTTACCGTGATGGCGGCGTTTCCGCTGTGTCCGGCTTGTGACAAAGAGTACCGCGACCCGCTCGA
TCGTCGCTTCCACGCCCAGCCGGTGGCCTGCCCGGAGTGTGGCCCACATCTTGAATGGGTAAGTCATGGTGAACATGCAG
AACAAGAGGCGGCATTACAGGCGGCTATCGCACAGTTAAAAATGGGCAACATTGTCGCCATCAAAGGGATTGGCGGATTT
CATCTCGCCTGCGATGCGCGTAACAGTAACGCGGTGGCGACACTACGGGCACGTAAACATCGCCCGGCGAAACCGCTGGC
GGTCATGTTGCCAGTGGCTGACGGTTTACCAGACGCTGCGCGCCAGTTGCTTTCCACGCCCGCCGCGCCGATTGTGCTGG
TGGATAAAAAATACATACCTGAGCTTTGTGATGATATCGCCCCTGGCCTTAACGAAGTTGGGGTGATGTTGCCTGCGAAC
CCGCTCCAGCATTTGCTGTTACAGGAACTGCAATGCCCGCTGGTGATGACCTCCGGCAACCTGAGCGGTAAACCACCAGC
TATCAGCAACGAACAGGCGCTGGCGGATTTGCAGGGCATTGCCGACGGATTCTTGATACATAACCGCGACATCGTGCAGC
GGATGGATGATTCGGTGGTGCGCGAAAGCGGCGAAATGCTGCGCCGTTCGCGGGGGTATGTGCCGGATGCGCTGGCTTTG
CCTCCGGGCTTTAAAAATGTTCCGCCTGTGCTGTGTCTCGGCGCGGATCTGAAAAATACCTTCTGCCTGGTGCGCGGTGA
ACAAGCGGTGTTGAGTCAGCATCTGGGCGATTTAAGTGACGATGGCATCCAGATGCAGTGGCGCGAAGCGTTACGCCTGA
TGCAAAACATCTACGATTTTACCCCGCAATACGTTGTGCATGACGCACATCCGGGCTATGTCTCCAGCCAGTGGGCGCGC
GAAATGAATCTGCCGACGCAAACGGTGCTGCATCATCATGCCCACGCGGCGGCGTGTCTGGCAGAACATCACTGGCCACT
GGAGGGCGGTGATGTCATTGCTTTGACGCTCGACGGTATCGGTATGGGGGAGAACGGCGCTTTGTGGGGCGGCGAGTGTC
TGCGGGTGAACTATCGCGAATGCCAGCACCTGGGCGGCTTGCCAGCGGTGGCGCTTGCTGGGGGCGATTTGGCTGCAAAG
CAGCCGTGGCGTAACCTGCTGGCGCAGTGCCTGCGCTTTGTGCCGGAGTGGCAGAATTACCCTGAAACGGCAAGTGTGCA
ACAGCAAAACTGGAGCGTGCTGGCGCGGGCCATTGAGCGTGGAATTAACGCGCCGCTGGCGTCATCGTGTGGGCGTTTGT
TTGATGCTGTGGCGGCGGCACTGGGCTGTGCGCCAGCCACGTTAAGTTATGAAGGTGAAGCGGCTTGTGCTCTGGAGGCG
CTCGCAGCTTCATGCGACGGAGTGACGCATCCGGTGACGATGCCGCTGGTGGACAATCAACTGGATCTCGCCACTTTCTG
GCAGCAGTGGCTGAACTGGCAGGCACCGGTTAATCAACGCGCGTGGGCGTTTCATGATGCGCTGGCGCAGGGTTTTGCCG
CGTTGATGCGTGAGCAGGCCACGATGCGCGGTATCACTACGCTGGTATTTAGCGGTGGGGTTATTCATAACCGATTGCTG
CGTACACGCCTGGCGCATTATCTCGCTGATTTCACATTACTCTTTCCGCAGAGTTTACCGGCGGGTGATGGCGGCTTGTC
TCTGGGGCAGGGGGTTATTGCTGCGGCGCGCATCCTGCATGCTGCATAG

Upstream 100 bases:

>100_bases
CTTACAGAACTGTTCTGGCATCAAAGGAATTCCCGGAAATATTCGTAGAATACGAAGCGGGCAGTATCGCCGACCTGTGA
CTGCCGGATGCGACGCTGTC

Downstream 100 bases:

>100_bases
TGGCGGATGTGGCGTAACTAAGCGTTAAAAGTAGCGCGGACAGTCCCCTCGCCCTTTGGGCGAGGGAGAAAACCATGCCT
ACATCGGCAACGTTTTCAAC

Product: [NiFe] hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 762; Mature: 761

Protein sequence:

>762_residues
MSGRAGYEIHDGGNNSMAKNISCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEAPETFLVQLHQH
CPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAM
PYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGF
HLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLSTPAAPIVLVDKKYIPELCDDIAPGLNEVGVMLPAN
PLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALAL
PPGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAR
EMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAK
QPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
LAASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLL
RTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARILHAA

Sequences:

>Translated_762_residues
MSGRAGYEIHDGGNNSMAKNISCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEAPETFLVQLHQH
CPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAM
PYDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGF
HLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLSTPAAPIVLVDKKYIPELCDDIAPGLNEVGVMLPAN
PLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALAL
PPGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAR
EMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAK
QPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
LAASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLL
RTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARILHAA
>Mature_761_residues
SGRAGYEIHDGGNNSMAKNISCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGDGVEVRLLEAPETFLVQLHQHC
PPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQIVPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMP
YDRPFTVMAAFPLCPACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGFH
LACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLSTPAAPIVLVDKKYIPELCDDIAPGLNEVGVMLPANP
LQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALP
PGFKNVPPVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWARE
MNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGENGALWGGECLRVNYRECQHLGGLPAVALAGGDLAAKQ
PWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEAL
AASCDGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLLR
TRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARILHAA

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=743, Percent_Identity=97.5773889636608, Blast_Score=1395, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 83028; Mature: 82897

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRAGYEIHDGGNNSMAKNISCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
CCCCCCCEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
GVEVRLLEAPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQIVP
CCEEEEECCCHHHHHHHHHHCCCHHHHCCCCCCCEEHHCCCCCEEEEECCCCEECCEECC
DAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDK
CCHHHHHHHHHCCCCCCCEEECCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCH
EYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGF
HHCCHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCE
HLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLSTPAAPIVLVDKKYIP
EEEEECCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEECHHHHH
ELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGI
HHHHHHCCCHHHCCEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
ADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGADLKNT
HCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
FCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAR
EEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
EMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGENGALWGGECLRVNYRE
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHH
CQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIER
HHHCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCDGVTHPVTMPLVDNQ
CCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEECCC
LDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLL
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARILHAA
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SGRAGYEIHDGGNNSMAKNISCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
CCCCCCEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCC
GVEVRLLEAPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQIVP
CCEEEEECCCHHHHHHHHHHCCCHHHHCCCCCCCEEHHCCCCCEEEEECCCCEECCEECC
DAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCPACDK
CCHHHHHHHHHCCCCCCCEEECCEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCH
EYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAIKGIGGF
HHCCHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCE
HLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLSTPAAPIVLVDKKYIP
EEEEECCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCEEEECHHHHH
ELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISNEQALADLQGI
HHHHHHCCCHHHCCEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHHH
ADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVPPVLCLGADLKNT
HCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
FCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVVHDAHPGYVSSQWAR
EEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHH
EMNLPTQTVLHHHAHAAACLAEHHWPLEGGDVIALTLDGIGMGENGALWGGECLRVNYRE
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEECCCEEEECHHH
CQHLGGLPAVALAGGDLAAKQPWRNLLAQCLRFVPEWQNYPETASVQQQNWSVLARAIER
HHHCCCCCEEEEECCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
GINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEALAASCDGVTHPVTMPLVDNQ
CCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEECEECCC
LDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMREQATMRGITTLVFSGGVIHNRLL
CHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RTRLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAARILHAA
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8661925; 12206761 [H]