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The map label for this gene is dusC [H]

Identifier: 170680883

GI number: 170680883

Start: 2315324

End: 2316271

Strand: Reverse

Name: dusC [H]

Synonym: EcSMS35_2287

Alternate gene names: 170680883

Gene position: 2316271-2315324 (Counterclockwise)

Preceding gene: 170680415

Following gene: 170682362

Centisome position: 45.7

GC content: 53.16

Gene sequence:

>948_bases
ATGCGTGTGTTACTGGCACCGATGGAGGGTGTACTCGACTCTCTGGTGCGTGAATTGCTGACCGAAGTTAACGACTACGA
TCTGTGCATCACCGAGTTTGTCCGCGTGGTGGATCAACTGCTGCCGGTAAAAGTCTTTCATCGCATTTGCCCTGAGCTAC
AAAACGCCAGCCGGACACCATCCGGTACGCTGGTGCGCGTGCAGTTGTTAGGTCAGTTCCCACAATGGCTGGCAGAGAAT
GCCGCCCGTGCGGTCGAGTTAGGTTCCTGGGGCGTGGATCTCAATTGCGGCTGCCCGTCGAAAACGGTTAACGGTAGCGG
CGGTGGGGCGACGTTACTCAAAGATCCTGAACTCATCTATCAGGGTGCAAAAGCGATGCGCGAAGCTGTACCGGCGCATT
TGCCCGTCAGCGTAAAAGTGCGTCTGGGCTGGGACAGCGGTGAGAAGAAATTTGAAATCGCCGATGCGGTTCAACAGGCT
GGTGCTACAGAGCTGGTGGTGCATGGGCGGACGAAAGAGCAGGGTTACCGCGCGGAGCATATTGACTGGCAGGCGATTGG
CGAGATTCGCCAGCGGCTGAATATTCCGGTGATTGCCAACGGTGAAATCTGGAACTGGCAGAGTGCGCAACAATGCATGA
CGATCAGCGGATGCGACGCAGTGATGATTGGTCGCGGGGCGCTCAATATTCCCAACCTGAGCCGGGTGGTAAAATATAAC
GAACCGCGAATGCCGTGGCCGGAGGTAGTTGCTTTGCTGCAAAAATATACCCGTCTGGAAAAGCAGGGCGATACCGGGTT
ATATCACGTAGCGCGGATTAAACAGTGGTTGAGTTATTTGCGTAAAGAATACGATGAAGCAACAGAATTATTTCAGCATG
TTCGGATGTTGAATAATTCCCCTGATATTGCAAGGGCTATTCAGGCAATTGATATCGAGAAACTCTAA

Upstream 100 bases:

>100_bases
ATAGAGAGGGATTGCAGTTTGCAGCAATCCCTCTTTGCAGACCTTTAATCCGTTGACTATAGGCGCTAACATAGCGCCTC
ATTTTTTTGCGGGTGATGAT

Downstream 100 bases:

>100_bases
GAAACATAGTGATGTTTTTTGACAATGTGTTAGCTCTTTATCAATAGGCCGCACGGTTGTTTGGTGGCGTCAAAATCGCC
GAGGCGTTCCCTGAAGGCCG

Product: tRNA-dihydrouridine synthase C

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWNWQSAQQCMTISGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRMLNNSPDIARAIQAIDIEKL

Sequences:

>Translated_315_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWNWQSAQQCMTISGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRMLNNSPDIARAIQAIDIEKL
>Mature_315_residues
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAEN
AARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWNWQSAQQCMTISGCDAVMIGRGALNIPNLSRVVKYN
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRMLNNSPDIARAIQAIDIEKL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusC subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=231, Percent_Identity=31.1688311688312, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI8923374, Length=269, Percent_Identity=28.6245353159851, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI239788483, Length=225, Percent_Identity=32, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI239788462, Length=225, Percent_Identity=32, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI40807366, Length=239, Percent_Identity=28.8702928870293, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788462, Length=315, Percent_Identity=98.7301587301587, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1789660, Length=324, Percent_Identity=28.3950617283951, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI145693211, Length=251, Percent_Identity=25.8964143426295, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=168, Percent_Identity=39.2857142857143, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17507177, Length=154, Percent_Identity=35.0649350649351, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=218, Percent_Identity=26.605504587156, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=201, Percent_Identity=31.3432835820896, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=146, Percent_Identity=27.3972602739726, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19921524, Length=145, Percent_Identity=37.2413793103448, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24585320, Length=217, Percent_Identity=30.8755760368664, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24580595, Length=179, Percent_Identity=30.1675977653631, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920448, Length=179, Percent_Identity=30.1675977653631, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35275; Mature: 35275

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTP
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIY
CCCEEEHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHH
QGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
IDWQAIGEIRQRLNIPVIANGEIWNWQSAQQCMTISGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHCCCCEEECCCEECCCCHHHHEEECCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRMLNNS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIARAIQAIDIEKL
CHHHHHHHHHHCCCC
>Mature Secondary Structure
MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVKVFHRICPELQNASRTP
CCEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKTVNGSGGGATLLKDPELIY
CCCEEEHHHHHHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCCCCCCCCEEECCHHHHH
QGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQAGATELVVHGRTKEQGYRAEH
HHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCC
IDWQAIGEIRQRLNIPVIANGEIWNWQSAQQCMTISGCDAVMIGRGALNIPNLSRVVKYN
CCHHHHHHHHHHCCCCEEECCCEECCCCHHHHEEECCCCEEEECCCCCCCCCHHHHHCCC
EPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQWLSYLRKEYDEATELFQHVRMLNNS
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PDIARAIQAIDIEKL
CHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]