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The map label for this gene is pykA

Identifier: 170680822

GI number: 170680822

Start: 1321014

End: 1322456

Strand: Reverse

Name: pykA

Synonym: EcSMS35_1332

Alternate gene names: 170680822

Gene position: 1322456-1321014 (Counterclockwise)

Preceding gene: 170683767

Following gene: 170682668

Centisome position: 26.09

GC content: 52.6

Gene sequence:

>1443_bases
ATGTCCAGAAGGCTTCGCAGAACTAAAATCGTTACCACGTTAGGCCCGGCAACAGATCGCGATAATAACCTTGAAAAAGT
TATCGCGGCGGGTGCCAACGTTGTACGTATGAATTTTTCTCACGGCTCGCCTGAAGATCACAAAATGCGCGCGGATAAAG
TTCGTGAGATTGCCGCAAAACTGGGGCGTCATGTGGCTATTCTGGGTGACCTCCAGGGGCCCAAAATCCGTGTATCCACC
TTTAAAGAAGGCAAAGTTTTCCTCAATATTGGGGATAAATTCCTGCTCGACGCCAACCTGGGTAAAGGTGAAGGCGACAA
AGAAAAAGTCGGTATCGACTACAAAGGCCTGCCTGCTGACGTTGTGCCTGGTGACATCCTGCTGCTGGACGATGGTCGCG
TCCAGTTAAAAGTACTGGAAGTCCAGGGCATGAAAGTGTTCACCGAAGTGACCGTCGGTGGTCCCCTCTCCAACAATAAA
GGTATCAACAAACTTGGCGGCGGTTTGTCGGCTGAAGCGCTGACCGAAAAAGACAAAGCAGACATTAAGACTGCGGCGTT
AATTGGCGTAGATTACCTGGCTGTCTCCTTCCCACGCTGCGGCGAAGATCTGAACTATGCCCGTCGCCTGGCACGCGATG
CAGGATGTGATGCGAAAATTGTTGCCAAGGTTGAACGTGCGGAAGCCGTATGCAGCCAGGATGCAATGGATGACATCATC
CTCGCCTCTGACGTGGTAATGGTTGCTCGCGGCGACCTCGGTGTGGAAATTGGCGATCCGGAACTGGTCGGCATTCAGAA
GGCGTTGATCCGTCGTGCACGTCAGCTTAACCGCGCAGTAATCACTGCGACCCAGATGATGGAGTCAATGATTACTAACC
CGATGCCGACGCGTGCAGAAGTCATGGACGTAGCAAACGCCGTTCTGGATGGTACTGACGCTGTGATGCTTTCTGCAGAA
ACTGCTGCTGGTCAGTATCCTTCAGAAACTGTTGCAGCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCAT
CAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATCGCCATGTCAGCAATGTACGCGGCTA
ACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGGTCGTACGGCGCTGATGACCTCCCGTATCAGCTCT
GGTCTGCCAATTTTCGCCATGTCACGCCATGAACGTACGCTGAACCTGACTGCTCTCTATCGCGGCGTTACGCCGGTGCA
CTTTGATAGCGCTAATGACGGCGTAGCAGCTGCCAGCGAAGCGGTTAATCTGCTGCGCGATAAAGGTTACTTGATGTCTG
GTGACCTGGTGATTGTCACCCAGGGCGACGTGATGAGTACCGTGGGTTCTACTAATACCACGCGTATTTTAACGGTAGAG
TAA

Upstream 100 bases:

>100_bases
ACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGATTTCATGTTCAAGCAACACCTGGTTGT
TTCAGTCAACGGAGTATTAC

Downstream 100 bases:

>100_bases
GTACGTTGCCGGATGCGGCGAAAACGCCTCATCCGGCCTACAGTTCAATGATAGTTCAACAGATTTCGAATCTTCTGAAG
CAAACTTGAACTTATCACAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-2

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISS
GLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE

Sequences:

>Translated_480_residues
MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVST
FKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDII
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISS
GLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE
>Mature_479_residues
SRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTF
KEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKG
INKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDIIL
ASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAET
AAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSG
LPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family

Homologues:

Organism=Homo sapiens, GI33286422, Length=502, Percent_Identity=37.0517928286853, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI33286420, Length=502, Percent_Identity=37.0517928286853, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=37.1717171717172, Blast_Score=248, Evalue=1e-65,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=35.2226720647773, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=35.2226720647773, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=35.8620689655172, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=35.8620689655172, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.8912133891213, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.8912133891213, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.4883720930233, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=100, Blast_Score=969, Evalue=0.0,
Organism=Escherichia coli, GI1787965, Length=488, Percent_Identity=36.8852459016393, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17544584, Length=489, Percent_Identity=36.40081799591, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=494, Percent_Identity=37.4493927125506, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI71984413, Length=494, Percent_Identity=37.4493927125506, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71984406, Length=494, Percent_Identity=37.4493927125506, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17506831, Length=494, Percent_Identity=37.4493927125506, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319279, Length=490, Percent_Identity=36.1224489795918, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=499, Percent_Identity=34.8697394789579, Blast_Score=250, Evalue=3e-67,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=37.246963562753, Blast_Score=264, Evalue=9e-71,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=37.246963562753, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24648966, Length=418, Percent_Identity=36.1244019138756, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI24581235, Length=491, Percent_Identity=32.1792260692464, Blast_Score=225, Evalue=7e-59,
Organism=Drosophila melanogaster, GI24646914, Length=311, Percent_Identity=34.0836012861736, Blast_Score=140, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KPYK2_ECOLI (P21599)

Other databases:

- EMBL:   M63703
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M77039
- EMBL:   M87660
- PIR:   S29790
- RefSeq:   AP_002474.1
- RefSeq:   NP_416368.1
- ProteinModelPortal:   P21599
- SMR:   P21599
- DIP:   DIP-10622N
- MINT:   MINT-1238649
- STRING:   P21599
- PRIDE:   P21599
- EnsemblBacteria:   EBESCT00000004674
- EnsemblBacteria:   EBESCT00000014499
- GeneID:   946527
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1843
- KEGG:   eco:b1854
- EchoBASE:   EB0796
- EcoGene:   EG10803
- eggNOG:   COG0469
- GeneTree:   EBGT00050000009296
- HOGENOM:   HBG734376
- OMA:   SRSRQLN
- ProtClustDB:   PRK05826
- BioCyc:   EcoCyc:PKII-MONOMER
- BioCyc:   MetaCyc:PKII-MONOMER
- Genevestigator:   P21599
- GO:   GO:0005829
- GO:   GO:0006096
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806
- Gene3D:   G3DSA:2.40.33.10
- Gene3D:   G3DSA:3.20.20.60
- Gene3D:   G3DSA:3.40.1380.20
- PANTHER:   PTHR11817
- PRINTS:   PR01050
- TIGRFAMs:   TIGR01064

Pfam domain/function: PF00224 PK; PF02887 PK_C; SSF50800 PK_B_barrel_like; SSF52935 Pyruvate_kinase; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.7.1.40

Molecular weight: Translated: 51358; Mature: 51227

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: BINDING 36-36 BINDING 251-251 BINDING 252-252 BINDING 284-284

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAK
CCCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCC
VVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCHHH
DIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDII
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
VEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALY
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEHHHHCCCCCEEEECCCCCEEEEEEEE
RGVTPVHFDSANDGVAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEECCEEEEECCCHHHHCCCCCCEEEEEEC
>Mature Secondary Structure 
SRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSPEDHKMRADKVREIAAK
CCCCCHHEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
LGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGKGEGDKEKVGIDYKGLPAD
HCCEEEEEECCCCCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCEECCCCCCCCCC
VVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNKGINKLGGGLSAEALTEKDKA
CCCCCEEEEECCCEEEEEEEECCEEEEEEEEECCCCCCCCCCHHHCCCCCHHHHCCCHHH
DIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDAKIVAKVERAEAVCSQDAMDDII
HHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LASDVVMVARGDLGVEIGDPELVGIQKALIRRARQLNRAVITATQMMESMITNPMPTRAE
HHCCEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VMDVANAVLDGTDAVMLSAETAAGQYPSETVAAMARVCLGAEKIPSINVSKHRLDVQFDN
HHHHHHHHHCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHCHHHCCCCCCCCEEEEEEECC
VEEAIAMSAMYAANHLKGVTAIITMTESGRTALMTSRISSGLPIFAMSRHERTLNLTALY
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEHHHHCCCCCEEEECCCCCEEEEEEEE
RGVTPVHFDSANDGVAAASEAVNLLRDKGYLMSGDLVIVTQGDVMSTVGSTNTTRILTVE
CCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEECCEEEEECCCHHHHCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1732206; 1859631; 8439398