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The map label for this gene is gltT [H]

Identifier: 170680220

GI number: 170680220

Start: 753570

End: 754850

Strand: Reverse

Name: gltT [H]

Synonym: EcSMS35_0746

Alternate gene names: 170680220

Gene position: 754850-753570 (Counterclockwise)

Preceding gene: 170680962

Following gene: 170679952

Centisome position: 14.89

GC content: 48.95

Gene sequence:

>1281_bases
ATGAAGAAAATAAGTTTAACCACGATGATTCTTTTGGCGCTGGTACTTGGAATGATTATCGGCGTAGTGCTCAATAACAC
TGCTTCACCGGAAACCGCAAAACTCTATGCGCAAGAAATATCGATATTCACGACGATTTTCTTACGACTGATAAAAATGA
TTATCGCTCCGTTAGTGGTCTCTACCCTGGTGGTAGGTATAGCTAAAATGGGAGATGCCAAAGCCCTTGGTCGTATTTTT
TCTAAAACACTCTTTTTATTTATTTGCGCCTCATTGCTGTCAATCGCCTTAGGCTTGATAACAGTAAATTTCTTCATGCC
AGGCACAGGAATTAATTTTGTTGCACACGGAGCCGAAACCACCGGAGTGGTCGCGTCAGAACCCTTTACGCTAAAAGTAT
TTATTTCGCATGCTTTCCCCACCAGCATTGTCGATGCCATGGCGCACAATGAAATTTTGCAAATCGTGGTGTTCTCAATT
TTCCTCGGCTGTAGCCTGACGGCGATTGGTGAGAAAGGCAGCGCCATCGTTCACGCCTTAGATTCGCTGGCACATGCCAT
GTTAAAGCTCACTGGCTACGTCATGCTCTTCGCTCCCCTGACCGTATTCGCCGCTATTTCAGCATTGATTGCTGAACGAG
GACTGGCAGTTATGGTGAGCGCCGGGATCTTTATGGGTGAATTTTATTTCACCATGTTGTTACTTTGGGTGCTGCTTATC
GGTCTGGCCATCGTTTATGTCGGCCCCTGCATCAGACGCCTGACCCGTGCCCTTTCGGAACCCGCCCTGCTGGCATTTAC
CACATCCAGTTCTGAAGCGGCTTTTCCGGGAACGCTTGAAAAACTGGAGCAATTTGGCGTTTCCCCCAAAATTGCCAGCT
TTGTCTTACCCATTGGCTACTCATTTAATCTCGTTGGATCAATGGCCTACTGCTCCTTCGCCACAGTTTTCATCGCCCAG
GCCTGCAATATCCATTTATCCATCGGTGAGCAAATCACCATGCTGTTGATCCTGATGTTGACCTCGAAAGGAATGGCTGG
CGTACCACGCGCCTCAATGGTGGTTATCGCCGCCACGCTCAACCAGTTCAATATTCCGGAAGCGGGGCTGATCTTGCTGA
TGGGCGTTGATCCGTTCCTTGATATGGGGCGTTCCGCGACAAACGTCATGAGCAACGCAATGGGCGCTGCGATGGTGAGT
CGGTGGGAAGGCGAACATTTCGGCGAGGGCTGTCGGGGTAAAGCATTAAAACCCAATGAATCGAACGTTGCTCTGCCCTG
A

Upstream 100 bases:

>100_bases
TATTTGTAAGGTTGTAATAGCAGGTAGATGCCTGAATCTCTATTCAGGCTTCTACTTCTCCTGATTCTGATTTATCGCCG
TGCACCTGATGGAGGAAAAA

Downstream 100 bases:

>100_bases
GGGGGATCTTAAGCAATCAGAAGCCAATGACTACGCATTTTTATAAGCCGGATCATGAAGTTAGACGGCAATTAAGTAAA
ACTAACGTTTAAGGTGAAAA

Product: dicarboxylate/amino acid:cation family protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIF
SKTLFLFICASLLSIALGLITVNFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTMLLLWVLLI
GLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQ
ACNIHLSIGEQITMLLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAMVS
RWEGEHFGEGCRGKALKPNESNVALP

Sequences:

>Translated_426_residues
MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIF
SKTLFLFICASLLSIALGLITVNFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTMLLLWVLLI
GLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQ
ACNIHLSIGEQITMLLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAMVS
RWEGEHFGEGCRGKALKPNESNVALP
>Mature_426_residues
MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKMIIAPLVVSTLVVGIAKMGDAKALGRIF
SKTLFLFICASLLSIALGLITVNFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSI
FLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTMLLLWVLLI
GLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSFATVFIAQ
ACNIHLSIGEQITMLLILMLTSKGMAGVPRASMVVIAATLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAMVS
RWEGEHFGEGCRGKALKPNESNVALP

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=463, Percent_Identity=27.645788336933, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI21314632, Length=423, Percent_Identity=28.1323877068558, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI194239697, Length=501, Percent_Identity=25.1497005988024, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4827012, Length=284, Percent_Identity=31.3380281690141, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI66773030, Length=454, Percent_Identity=25.7709251101322, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI40254478, Length=458, Percent_Identity=24.6724890829694, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI5032093, Length=390, Percent_Identity=28.2051282051282, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI223468566, Length=260, Percent_Identity=28.8461538461538, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI223468564, Length=261, Percent_Identity=28.735632183908, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI262359914, Length=400, Percent_Identity=26.25, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI301601644, Length=196, Percent_Identity=30.6122448979592, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1790514, Length=422, Percent_Identity=39.0995260663507, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789947, Length=372, Percent_Identity=36.5591397849462, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1788024, Length=398, Percent_Identity=24.8743718592965, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17537407, Length=449, Percent_Identity=28.9532293986637, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI71996953, Length=486, Percent_Identity=25.3086419753086, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71983099, Length=461, Percent_Identity=26.0303687635575, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI71983106, Length=469, Percent_Identity=25.5863539445629, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI193206505, Length=445, Percent_Identity=26.7415730337079, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI193206654, Length=448, Percent_Identity=24.7767857142857, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17541374, Length=136, Percent_Identity=34.5588235294118, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583025, Length=458, Percent_Identity=26.2008733624454, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17137668, Length=458, Percent_Identity=26.2008733624454, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24583023, Length=458, Percent_Identity=26.2008733624454, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281360483, Length=464, Percent_Identity=26.5086206896552, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137666, Length=464, Percent_Identity=26.5086206896552, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI281360481, Length=464, Percent_Identity=26.5086206896552, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 45277; Mature: 45277

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKMIIAPLVV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITVNFFMPGTGINFVAHGAET
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCC
TGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSIFLGCSLTAIGEKGSAIVHAL
CCEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHH
DSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTMLLLWVLLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHH
GLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIASFVLPIGY
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCC
SFNLVGSMAYCSFATVFIAQACNIHLSIGEQITMLLILMLTSKGMAGVPRASMVVIAATL
CHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEHH
NQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAMVSRWEGEHFGEGCRGKALKPNE
CCCCCCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
SNVALP
CCCCCC
>Mature Secondary Structure
MKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKMIIAPLVV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITVNFFMPGTGINFVAHGAET
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCC
TGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSIFLGCSLTAIGEKGSAIVHAL
CCEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCHHHHHHH
DSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTMLLLWVLLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHH
GLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIASFVLPIGY
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCC
SFNLVGSMAYCSFATVFIAQACNIHLSIGEQITMLLILMLTSKGMAGVPRASMVVIAATL
CHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHCCCCCCCCCHHHEEEEEHH
NQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAMVSRWEGEHFGEGCRGKALKPNE
CCCCCCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
SNVALP
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]