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The map label for this gene is fumB [H]

Identifier: 170679952

GI number: 170679952

Start: 751810

End: 753462

Strand: Reverse

Name: fumB [H]

Synonym: EcSMS35_0745

Alternate gene names: 170679952

Gene position: 753462-751810 (Counterclockwise)

Preceding gene: 170680220

Following gene: 170683867

Centisome position: 14.87

GC content: 49.18

Gene sequence:

>1653_bases
ATGTCCAAACCATTTATCTGGCAAGAACCGTTTCTACAAAATAAAGACGGTACAGAATATACATTAATTAGCGATCAACA
TATTACAGTAACGGAATTAGACGGCGAAGAAGTGATAAAAATCGCTCCTGAAGCATTAACGCTGCTTGCCCGGCAGGCAT
TTTATGAAGCCTCTTTTTTCTTACGTTCCGCTCATTTGCAACAGGTCGCCAGCATTCTTAACGATCCGCACGCCAGTAGT
AATGATAAATACGTTGCGTTGCAGTTATTACGTAATGCTGAAGTCTCAGCAAAAGGGGTATTACCCAACTGTCAGGATAC
CGGCACCGCAACTATTGTTGCCAGTAAAGGGCAACAGATATGGACCGGAGGTAACGACGCTGAAGCCTTAAGCAAAGGCA
TTTACACCACTTTTCAGGAAAATAATCTGCGCTTTTCACAAAATGCGCCACTGGATATGTTTACCGAAGTCAATACGCAA
ACCAATCTCCCGGCGCAGATTGATATCAGCGCCGTCGCTGGTGATGAGTACCATTTTCTCTGTATTAATAAAGGGGGCGG
CTCTGCCAATAAAGCCGCGCTTTATCAGGAAACCAAATCCCTATTGCAACCAGAAAAACTGACCGCATTTTTAATCGAAA
AAATGAAATCGCTGGGCACCGCTGCCTGCCCGCCTTACCACATCGCCTTTGTCGTCGGAGGGCTTTCAGCCGATCAAACC
TTAAAAGTTGCCAAGCTGGCCTCAACGAAATATTACGACAATTTGCCCACCAGCGGAAACGAGCAAGGACAAGCATTCCG
TGATATTGAGCTGGAAAAAGTATTGCTCGAGGCCAGTCAGCAATTTGGTATTGGTGCGCAATTTGGGGGTAAATATTTCG
CTCATGATATTCGCGTCATTCGTCTGCCGCGTCACGGTGGCTCTTGCCCGATAGCTATGGCGCTCTCCTGTTCTGCGGAT
CGCAATATCAAAGCAAAAATTAACAAGCACGGTATCTGGCTGGAAAAACTCGAACACAATCCGGGGCAATATATTCCAGC
CTCACTGCGGGAAGAAAATCACGCGCAACATGTACAGCTGGATCTAAACCGTCCGTTGCGTGACGTGATGCAGGATCTCG
CCAGATTACCGGTAGGCACCCGCGTGTCGTTAAGTGGTCCCATCGTAGTGGCGCGCGATATCGCCCACGCCAAAATAAAA
GCGCGGCTGGATAGCGGCGAACCGATGCCGGATTACCTGAAGCATCATATTGTCTATTACGCTGGCCCGGCGAAAACACC
GGAGAACATGGCCTGCGGTTCACTGGGTCCAACCACGGGCGGTCGAATGGACGGTTATGTCGATACCTTCCAGGCGGCTG
GCGGCAGTCTGGTGATGTTATCGAAAGGTAACCGCAGCCAGCAGGTCACCGATGCCTGTCACAAACATGGCGGCTTCAAT
CTGGGGAGTATTGGCGGTGCAGCTGCGTTACTGGCGCAAGAATATGTCAAAAGTCTGCGTTGCCTGGAATACCCGGAGCT
GGGTATGGAAGCGGTGTGGATGATGGAAGTGGAAAATTTACCCGCATTCATCCTGGTAGATGATAAAGGCAATAATTTCT
TTAGTCAGTTTGAGCAGCAACACCGCTGCGCCAGTTGCCCTGCGGGACATTAA

Upstream 100 bases:

>100_bases
CTTAAGCAATCAGAAGCCAATGACTACGCATTTTTATAAGCCGGATCATGAAGTTAGACGGCAATTAAGTAAAACTAACG
TTTAAGGTGAAAAGTGAAAT

Downstream 100 bases:

>100_bases
GGAAAATGAATGGAAGCGCGAATTTCAACTGAACGGCATGTGCAGCGCCAGCAGAAATTGAAGGAACAGGTCGATACGCG
CGTCGCGGCGGCGATAGAGA

Product: tartrate/fumarate family Fe-S type hydro-lyase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 550; Mature: 549

Protein sequence:

>550_residues
MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPEALTLLARQAFYEASFFLRSAHLQQVASILNDPHASS
NDKYVALQLLRNAEVSAKGVLPNCQDTGTATIVASKGQQIWTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMFTEVNTQ
TNLPAQIDISAVAGDEYHFLCINKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSLGTAACPPYHIAFVVGGLSADQT
LKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLLEASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSAD
RNIKAKINKHGIWLEKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVARDIAHAKIK
ARLDSGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTFQAAGGSLVMLSKGNRSQQVTDACHKHGGFN
LGSIGGAAALLAQEYVKSLRCLEYPELGMEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH

Sequences:

>Translated_550_residues
MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPEALTLLARQAFYEASFFLRSAHLQQVASILNDPHASS
NDKYVALQLLRNAEVSAKGVLPNCQDTGTATIVASKGQQIWTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMFTEVNTQ
TNLPAQIDISAVAGDEYHFLCINKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSLGTAACPPYHIAFVVGGLSADQT
LKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLLEASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSAD
RNIKAKINKHGIWLEKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVARDIAHAKIK
ARLDSGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTFQAAGGSLVMLSKGNRSQQVTDACHKHGGFN
LGSIGGAAALLAQEYVKSLRCLEYPELGMEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH
>Mature_549_residues
SKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPEALTLLARQAFYEASFFLRSAHLQQVASILNDPHASSN
DKYVALQLLRNAEVSAKGVLPNCQDTGTATIVASKGQQIWTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMFTEVNTQT
NLPAQIDISAVAGDEYHFLCINKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSLGTAACPPYHIAFVVGGLSADQTL
KVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLLEASQQFGIGAQFGGKYFAHDIRVIRLPRHGGSCPIAMALSCSADR
NIKAKINKHGIWLEKLEHNPGQYIPASLREENHAQHVQLDLNRPLRDVMQDLARLPVGTRVSLSGPIVVARDIAHAKIKA
RLDSGEPMPDYLKHHIVYYAGPAKTPENMACGSLGPTTGGRMDGYVDTFQAAGGSLVMLSKGNRSQQVTDACHKHGGFNL
GSIGGAAALLAQEYVKSLRCLEYPELGMEAVWMMEVENLPAFILVDDKGNNFFSQFEQQHRCASCPAGH

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=545, Percent_Identity=68.4403669724771, Blast_Score=815, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=545, Percent_Identity=66.9724770642202, Blast_Score=803, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=179, Percent_Identity=27.3743016759777, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60076; Mature: 59945

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPEALTLLARQAFYEASFF
CCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHEEECHHHHHHHHHHHHHHHHHH
LRSAHLQQVASILNDPHASSNDKYVALQLLRNAEVSAKGVLPNCQDTGTATIVASKGQQI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCEEEEECCCCEE
WTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMFTEVNTQTNLPAQIDISAVAGDEYHFL
EECCCHHHHHHHCCEEEEECCCEEECCCCCCCCEEECCCCCCCCCEEEEEEEECCCEEEE
CINKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSLGTAACPPYHIAFVVGGLSADQT
EEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCH
LKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLLEASQQFGIGAQFGGKYFAHDIRVI
HHHHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEECEEEE
RLPRHGGSCPIAMALSCSADRNIKAKINKHGIWLEKLEHNPGQYIPASLREENHAQHVQL
EECCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHCCCCCCEEEEE
DLNRPLRDVMQDLARLPVGTRVSLSGPIVVARDIAHAKIKARLDSGEPMPDYLKHHIVYY
ECCCCHHHHHHHHHHCCCCCEEECCCCEEEEECCHHEEEEEECCCCCCCHHHHHCCEEEE
AGPAKTPENMACGSLGPTTGGRMDGYVDTFQAAGGSLVMLSKGNRSQQVTDACHKHGGFN
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCC
LGSIGGAAALLAQEYVKSLRCLEYPELGMEAVWMMEVENLPAFILVDDKGNNFFSQFEQQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHH
HRCASCPAGH
HHHCCCCCCC
>Mature Secondary Structure 
SKPFIWQEPFLQNKDGTEYTLISDQHITVTELDGEEVIKIAPEALTLLARQAFYEASFF
CCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCHHEEECHHHHHHHHHHHHHHHHHH
LRSAHLQQVASILNDPHASSNDKYVALQLLRNAEVSAKGVLPNCQDTGTATIVASKGQQI
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCEEEEECCCCEE
WTGGNDAEALSKGIYTTFQENNLRFSQNAPLDMFTEVNTQTNLPAQIDISAVAGDEYHFL
EECCCHHHHHHHCCEEEEECCCEEECCCCCCCCEEECCCCCCCCCEEEEEEEECCCEEEE
CINKGGGSANKAALYQETKSLLQPEKLTAFLIEKMKSLGTAACPPYHIAFVVGGLSADQT
EEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCH
LKVAKLASTKYYDNLPTSGNEQGQAFRDIELEKVLLEASQQFGIGAQFGGKYFAHDIRVI
HHHHHHHCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEECEEEE
RLPRHGGSCPIAMALSCSADRNIKAKINKHGIWLEKLEHNPGQYIPASLREENHAQHVQL
EECCCCCCCCEEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHCCCCCCEEEEE
DLNRPLRDVMQDLARLPVGTRVSLSGPIVVARDIAHAKIKARLDSGEPMPDYLKHHIVYY
ECCCCHHHHHHHHHHCCCCCEEECCCCEEEEECCHHEEEEEECCCCCCCHHHHHCCEEEE
AGPAKTPENMACGSLGPTTGGRMDGYVDTFQAAGGSLVMLSKGNRSQQVTDACHKHGGFN
ECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCC
LGSIGGAAALLAQEYVKSLRCLEYPELGMEAVWMMEVENLPAFILVDDKGNNFFSQFEQQ
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHHH
HRCASCPAGH
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]