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The map label for this gene is dmsA [H]

Identifier: 170680161

GI number: 170680161

Start: 2243822

End: 2246266

Strand: Reverse

Name: dmsA [H]

Synonym: EcSMS35_2226

Alternate gene names: 170680161

Gene position: 2246266-2243822 (Counterclockwise)

Preceding gene: 170683015

Following gene: 170680702

Centisome position: 44.32

GC content: 52.19

Gene sequence:

>2445_bases
ATGAAAACGAAAATCCCTGATGCGGTATTGGCTGCTGAGGTGAGTCGCCGTGGTTTGGTAAAAACGACAGCGATCGGCGG
CCTGGCAATGGCCAGCAGCGCATTAACATTACCTTTTAGTCGGATTGCGCACGCTGTTGATAGTGCCCTTCCGGCAAAGT
CAGACGAAAAGGTAATCTGGAGCGCCTGTACAGTTAACTGTGGTAGCCGCTGCCCGCTACGTATGCACGTTGTGGACGGT
GAAATTAAATATGTTGAAACGGATAATACCGGCGATGACAACTATGACGGCCTGCACCAGGTGCGCGCCTGTCTGCGTGG
GCGTTCCATGCGTCGCCGTGTCTACAATCCGGACCGCCTGAAATATCCGATGAAACGAGTCGGGGCGCGCGGTGAAGGCA
AATTCGAGCGCATTAGCTGGGAAGAAGCCTACGACATCATCGCGACCAATATGCAGCGCCTGATCAAAGAGTACGGCAAC
GAGTCTATCTATCTGAACTATGGTACCGGTACGCTGGGCGGCACCATGACCCGCTCCTGGCCGCCGGGAAATACCCTGGT
CGCTCGCCTGATGAACTGCTGCGGCGGTTATCTGAACCATTACGGTGACTACTCTTCCGCGCAAATTGCGGAAGGTTTGA
ACTATACCTACGGCGGCTGGGCAGATGGTAACAGCCCGTCGGATATCGAAAACAGTAAACTGGTAGTGCTGTTTGGTAAT
AACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCGCGCCAGAAATCTAATGCTCGCAT
GATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATG
CCGCACTGGTTAACGGTCTGGCGTATGTCATGATCACCGAAAACCTGGTGGATCAGGCATTCCTCGATAAATATTGCGTT
GGCTACGACGAGAAAACACTGCCAGCCAGTGCGCCGAAAAATGGTCACTATAAAGCTTATATTCTGGGTGAAGGGCCAGA
TGGCGTGGCTAAAACGCCGGAATGGGCCTCGCAAATCACTGGTGTTCCGGCAGACAAAATCATCAAATTGGCTCGTGAAA
TCGGTAGTACCAAACCGGCGTTTATCAGCCAGGGATGGGGCCCGCAGCGTCACGCTAACGGTGAAATCGCAACCCGTGCT
ATCTCGATGCTGGCGATTCTGACCGGTAACGTTGGTATTAACGGAGGCAACAGCGGCGCGCGTGAAGGTTCATACAGCTT
ACCGTTTGTCCGTATGCCGACCCTCGAAAACCCGATCCAGACCAGCATTTCGATGTTTATGTGGACCGATGCCATTGAAC
GTGGCCCGGAAATGACAGCGCTGCGTGATGGTGTGCGCGGGAAAGATAAGCTGGATGTGCCGATCAAAATGATCTGGAAC
TATGCCGGTAACTGCCTGATTAATCAGCATTCTGAAATCAACCGTACCCATGAAATCCTTCAGGATGATAAGAAGTGCGA
GCTGATTGTGGTTATCGACTGCCACATGACCTCATCGGCGAAATATGCTGACATCCTGCTGCCTGACTGCACCGCTTCAG
AACAGATGGACTTTGCACTGGATGCATCCTGCGGGAATATGTCTTACGTGATTTTCAACGATCAGGTGATTAAACCGCGC
TTTGAATGTAAGACCATCTATGAAATGACCAGCGAACTGGCAAAACGTCTTGGCGTTGAGCAACAATTTACTGAGGGCCG
TACCCAGGAAGAGTGGATGCGGCATCTGTATGCCCAGTCGCGGGAAGCGATTCCTGAACTGCCAACGTTTGAAGAGTTCC
GTAAGCAGGGGATCTTTAAAAAGCGCGACCCACAAGGGCATCACGTTGCTTATAAAGCTTTCCGTGAAGATCCGCAGGCA
AATCCACTGACCACGCCATCGGGGAAAATTGAGATTTATTCGCAGGCGCTGGCTGACATTGCCGCGACCTGGGAATTGCC
AGAAGGTGATGTGATCGATCCACTGCCGATCTACACGCCGGGCTTTGAAAGTTATCAGGATCCGCTGAACAAACAGTATC
CGCTGCAGCTTACGGGTTTCCACTATAAATCTCGCGTTCACTCAACTTACGGCAACGTTGATGTGCTGAAAGCAGCTTGC
CGTCAGGAAATGTGGATCAACCCGCTTGATGCGCAAAAACGCGGTATCAACAACGGCGATAAAGTCAGGATCTTTAACGA
TCGTGGTGAGGTTCATATTGAGGCGAAAGTGACGCCACGAATGATGCCGGGTGTGGTCGCACTGGGTGAAGGTGCCTGGT
ATGACCCGGATACAAAACGTGTCGATAAGGGCGGTTGTATCAACGTACTGACCACTCAACGTCCGTCTCCTCTCGCTAAG
GGGAATCCGTCACATACAAACCTTGTTCAGGTTGAAAAGGTGTAA

Upstream 100 bases:

>100_bases
ATTCGATATATACAAGCCTATATAGCGACCTGCTATAGAAATAATTACACAATACGGTTTGTTACTGGAATCAATCGTGA
GCAAGCTTGAGTGAGCCATT

Downstream 100 bases:

>100_bases
GGAGTAACCGATGACAACCCAGTATGGATTTTTTATTGATTCCAGCCGTTGCACCGGTTGCAAAACCTGCGAGCTGGCCT
GTAAAGACTACAAAGATTTG

Product: anaerobic dimethyl sulfoxide reductase, A subunit

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: DMSO reductase [H]

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAHAVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDG
EIKYVETDNTGDDNYDGLHQVRACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYGGWADGNSPSDIENSKLVVLFGN
NPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTDTGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCV
GYDEKTLPASAPKNGHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHANGEIATRA
ISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTDAIERGPEMTALRDGVRGKDKLDVPIKMIWN
YAGNCLINQHSEINRTHEILQDDKKCELIVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPR
FECKTIYEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDPQGHHVAYKAFREDPQA
NPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGFESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAAC
RQEMWINPLDAQKRGINNGDKVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPLAK
GNPSHTNLVQVEKV

Sequences:

>Translated_814_residues
MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAHAVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDG
EIKYVETDNTGDDNYDGLHQVRACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYGGWADGNSPSDIENSKLVVLFGN
NPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTDTGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCV
GYDEKTLPASAPKNGHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHANGEIATRA
ISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTDAIERGPEMTALRDGVRGKDKLDVPIKMIWN
YAGNCLINQHSEINRTHEILQDDKKCELIVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPR
FECKTIYEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDPQGHHVAYKAFREDPQA
NPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGFESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAAC
RQEMWINPLDAQKRGINNGDKVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPLAK
GNPSHTNLVQVEKV
>Mature_814_residues
MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAHAVDSALPAKSDEKVIWSACTVNCGSRCPLRMHVVDG
EIKYVETDNTGDDNYDGLHQVRACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYGGWADGNSPSDIENSKLVVLFGN
NPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTDTGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCV
GYDEKTLPASAPKNGHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHANGEIATRA
ISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTDAIERGPEMTALRDGVRGKDKLDVPIKMIWN
YAGNCLINQHSEINRTHEILQDDKKCELIVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPR
FECKTIYEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDPQGHHVAYKAFREDPQA
NPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGFESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAAC
RQEMWINPLDAQKRGINNGDKVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPLAK
GNPSHTNLVQVEKV

Specific function: Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds. Allows E.coli to grow anaerobically on Me(2)SO as respiratory oxidant [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=814, Percent_Identity=99.5085995085995, Blast_Score=1694, Evalue=0.0,
Organism=Escherichia coli, GI1787870, Length=805, Percent_Identity=67.2049689440994, Blast_Score=1149, Evalue=0.0,
Organism=Escherichia coli, GI171474008, Length=818, Percent_Identity=65.5256723716381, Blast_Score=1135, Evalue=0.0,
Organism=Escherichia coli, GI145693196, Length=757, Percent_Identity=32.1003963011889, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI1787231, Length=761, Percent_Identity=32.0630749014455, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI87081994, Length=860, Percent_Identity=29.8837209302326, Blast_Score=278, Evalue=1e-75,
Organism=Escherichia coli, GI3868721, Length=783, Percent_Identity=25.0319284802043, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI3868720, Length=468, Percent_Identity=27.3504273504274, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1788534, Length=838, Percent_Identity=23.2696897374702, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI3868719, Length=476, Percent_Identity=25.6302521008403, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787477, Length=350, Percent_Identity=24.8571428571429, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1787741, Length=309, Percent_Identity=24.5954692556634, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011888
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 90377; Mature: 90377

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00490 MOLYBDOPTERIN_PROK_2 ; PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAHAVDSALPAKSDEKVIW
CCCCCCHHHHHHHHHHCCCEEHHHHCHHHHHCCHHCCCHHHHHHHHHHHCCCCCCCCEEE
SACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVRACLRGRSMRRRVYNPDRL
EEEECCCCCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCHHH
KYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGNESIYLNYGTGTLGGTMTRSW
CCCHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
PPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYGGWADGNSPSDIENSKLVVLFGN
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECC
NPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTDTGAGREDEWIPIRPGTDAALVNGL
CCCCCEECCCCEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHCCE
AYVMITENLVDQAFLDKYCVGYDEKTLPASAPKNGHYKAYILGEGPDGVAKTPEWASQIT
EEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHC
GVPADKIIKLAREIGSTKPAFISQGWGPQRHANGEIATRAISMLAILTGNVGINGGNSGA
CCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
REGSYSLPFVRMPTLENPIQTSISMFMWTDAIERGPEMTALRDGVRGKDKLDVPIKMIWN
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHH
YAGNCLINQHSEINRTHEILQDDKKCELIVVIDCHMTSSAKYADILLPDCTASEQMDFAL
CCCCEEEECCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCEEE
DASCGNMSYVIFNDQVIKPRFECKTIYEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQS
ECCCCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHH
REAIPELPTFEEFRKQGIFKKRDPQGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADI
HHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHH
AATWELPEGDVIDPLPIYTPGFESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAAC
HHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCHHHHHHHH
RQEMWINPLDAQKRGINNGDKVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKR
HHHHCCCCCCHHHCCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCC
VDKGGCINVLTTQRPSPLAKGNPSHTNLVQVEKV
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAHAVDSALPAKSDEKVIW
CCCCCCHHHHHHHHHHCCCEEHHHHCHHHHHCCHHCCCHHHHHHHHHHHCCCCCCCCEEE
SACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVRACLRGRSMRRRVYNPDRL
EEEECCCCCCCCEEEEEECCEEEEEECCCCCCCCCHHHHHHHHHHHCHHHHHHCCCCHHH
KYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGNESIYLNYGTGTLGGTMTRSW
CCCHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCC
PPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYGGWADGNSPSDIENSKLVVLFGN
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECC
NPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTDTGAGREDEWIPIRPGTDAALVNGL
CCCCCEECCCCEEHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEECCCCCCHHHHCCE
AYVMITENLVDQAFLDKYCVGYDEKTLPASAPKNGHYKAYILGEGPDGVAKTPEWASQIT
EEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHC
GVPADKIIKLAREIGSTKPAFISQGWGPQRHANGEIATRAISMLAILTGNVGINGGNSGA
CCCHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
REGSYSLPFVRMPTLENPIQTSISMFMWTDAIERGPEMTALRDGVRGKDKLDVPIKMIWN
CCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHH
YAGNCLINQHSEINRTHEILQDDKKCELIVVIDCHMTSSAKYADILLPDCTASEQMDFAL
CCCCEEEECCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCEEE
DASCGNMSYVIFNDQVIKPRFECKTIYEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQS
ECCCCCEEEEEECCCEECCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHH
REAIPELPTFEEFRKQGIFKKRDPQGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADI
HHCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHH
AATWELPEGDVIDPLPIYTPGFESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAAC
HHHCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEECCHHHHHHHHCCCCHHHHHHHH
RQEMWINPLDAQKRGINNGDKVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKR
HHHHCCCCCCHHHCCCCCCCEEEEECCCCCEEEEEEECCCCCCCEEEECCCCCCCCCCCC
VDKGGCINVLTTQRPSPLAKGNPSHTNLVQVEKV
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3062312; 8905232; 9278503; 2170332; 8125918; 10801884 [H]