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The map label for this gene is phrA [H]

Identifier: 170079261

GI number: 170079261

Start: 2796741

End: 2798285

Strand: Reverse

Name: phrA [H]

Synonym: SYNPCC7002_A2668

Alternate gene names: 170079261

Gene position: 2798285-2796741 (Counterclockwise)

Preceding gene: 170079263

Following gene: 170079259

Centisome position: 93.03

GC content: 52.17

Gene sequence:

>1545_bases
ATGTCTTCTGTCAATATTCTCCTGTGGTATCGCCGTGATCTCCGGCTCCATGACCACCAGCCACTCCAGGAAGCCCTAGC
ACAACAGGCCCAGATTTTTCCTTTCTACTGTTTTGACCCCCGGGGGTTTGGTTCAATTGGGCTAGATTTCCCGAAAACAG
GTGCCTTTCGGGGTCAGTTTCTCCGGGAAGCCATCCAGGATCTCCGGCAAAATCTCCGCGCCAAGGGCAGTGATTTGATT
GTGCGTCAGGATATCCCGGAAAAGTTAATCCCTGATTTATGTCAGCAATGGCAGATTGATGTTGTGTATTGTCACAGGGA
GGTAACGGCAGAGGAAACCCGCGTTGAACGAAAACTGCGCCAAGCTCTCCAAGAACAGGGTGTTCGAGTAGAAACGTTTT
GGGGAACGACCCTCCACGACCCAGGAGATTTGCCTTTTGGGATTAACGAAGTGCCGGAGGTTTTTACGCAATTTCGTAAG
GATGTGGAAAAGTATGCGGCGATCGCCTCCCCAGTACCCACACCAACCCAATTGCCTCCCCTCCCCGAAACCGTTGCTCC
AGGTGAGATTCCCAGTCTCCAGGATTTGGGTTTGCCGGAACCAACCATTGATCCCCGTGGTGTGCTCCCGCTCAAAGGCG
GTGAAACGGCTGGTATGAAACGGCTCCAGGCTTATTTTTGGGAAGGCGATCGCCTCAAGGTTTACAAAGAAACCCGCAAT
GGTTTGTTGGGAGCCGATTACTCCTCAAAATTTTCACCCTGGCTGGCCCTTGGTTGCCTGTCGGCCCGCTACATTGCTGC
CCAAGTCGCTGAATACGAACAAAAACGGATCGCTAACAATTCCACCTACTGGCTGATTTTTGAGCTGCTCTGGCGGGATT
ATTTCCGCTGGATCTGTGCCAAACACGGCAACCAAATTTTTCAACCTGAAGGTTTACAGGGGGTAAAAATTCCTTGGAAA
CAAGACCACGAAGCCTTTGAACTGTGGCGGACGGGCCAAACGGGGATTCCCTTCGTCGATGCCAATATGCGCGAACTCCT
GTATACGGGCTTTATGTCCAACCGGGGCCGGCAAAATGTCGGTAGTTTCCTGACGAAAAATCTGGGGATTGACTGGCGGT
GGGGGGCGGCTTGGTTTGAGTCACAACTCATTGACTACGATGTGTGCAGCAACTGGGGGAACTGGAATTACACCGCTGGC
GTAGGGAATGATGCGCGCGGCTTTCGCTATTTCAACATTCCCAAACAGGCCAAGGATTACGATCCCCAGGGGGACTATGT
GCGCCACTGGCTTCCGGAGTTGGCAGCCCTTCCCGGAGAGCAGATCCATCAACCCTGGCGTTTATCGGTAAAAGAGTTGG
GCGATCGCCAAATTATTTTGGGGGAAACCTACCCGAAACCGATGGTTGATCTGCAAACCTCTGTCCAAGAAAACGAACGC
ATTTACCAAAAAGCTCTGGGTCTGGCCCCTAAATCCCAATCCCGAAAGCCTCGTAAAAATTTCAAACGTAATCAAGACAC
TCCCAAGGCCCGTTGGCGGCGTTAG

Upstream 100 bases:

>100_bases
TTTTAAAACTCATAAAAATCATGCGTCCCCGAATCAATTGAATCTCCATGGCAATTAACGTTACCTTATGAGCCTCTACT
TTATCGTTCGCTAGATCACA

Downstream 100 bases:

>100_bases
TCTCCTGAAACGGGGCCGTTCAAAAAAACTTTTGCCTCTCCCCTTGCGTTTTAAAAAATCCCCTGTTATAGTTGTTAAGG
CTTAAGGGCGTTTAGCTCAG

Product: DNA photolyase

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSSVNILLWYRRDLRLHDHQPLQEALAQQAQIFPFYCFDPRGFGSIGLDFPKTGAFRGQFLREAIQDLRQNLRAKGSDLI
VRQDIPEKLIPDLCQQWQIDVVYCHREVTAEETRVERKLRQALQEQGVRVETFWGTTLHDPGDLPFGINEVPEVFTQFRK
DVEKYAAIASPVPTPTQLPPLPETVAPGEIPSLQDLGLPEPTIDPRGVLPLKGGETAGMKRLQAYFWEGDRLKVYKETRN
GLLGADYSSKFSPWLALGCLSARYIAAQVAEYEQKRIANNSTYWLIFELLWRDYFRWICAKHGNQIFQPEGLQGVKIPWK
QDHEAFELWRTGQTGIPFVDANMRELLYTGFMSNRGRQNVGSFLTKNLGIDWRWGAAWFESQLIDYDVCSNWGNWNYTAG
VGNDARGFRYFNIPKQAKDYDPQGDYVRHWLPELAALPGEQIHQPWRLSVKELGDRQIILGETYPKPMVDLQTSVQENER
IYQKALGLAPKSQSRKPRKNFKRNQDTPKARWRR

Sequences:

>Translated_514_residues
MSSVNILLWYRRDLRLHDHQPLQEALAQQAQIFPFYCFDPRGFGSIGLDFPKTGAFRGQFLREAIQDLRQNLRAKGSDLI
VRQDIPEKLIPDLCQQWQIDVVYCHREVTAEETRVERKLRQALQEQGVRVETFWGTTLHDPGDLPFGINEVPEVFTQFRK
DVEKYAAIASPVPTPTQLPPLPETVAPGEIPSLQDLGLPEPTIDPRGVLPLKGGETAGMKRLQAYFWEGDRLKVYKETRN
GLLGADYSSKFSPWLALGCLSARYIAAQVAEYEQKRIANNSTYWLIFELLWRDYFRWICAKHGNQIFQPEGLQGVKIPWK
QDHEAFELWRTGQTGIPFVDANMRELLYTGFMSNRGRQNVGSFLTKNLGIDWRWGAAWFESQLIDYDVCSNWGNWNYTAG
VGNDARGFRYFNIPKQAKDYDPQGDYVRHWLPELAALPGEQIHQPWRLSVKELGDRQIILGETYPKPMVDLQTSVQENER
IYQKALGLAPKSQSRKPRKNFKRNQDTPKARWRR
>Mature_513_residues
SSVNILLWYRRDLRLHDHQPLQEALAQQAQIFPFYCFDPRGFGSIGLDFPKTGAFRGQFLREAIQDLRQNLRAKGSDLIV
RQDIPEKLIPDLCQQWQIDVVYCHREVTAEETRVERKLRQALQEQGVRVETFWGTTLHDPGDLPFGINEVPEVFTQFRKD
VEKYAAIASPVPTPTQLPPLPETVAPGEIPSLQDLGLPEPTIDPRGVLPLKGGETAGMKRLQAYFWEGDRLKVYKETRNG
LLGADYSSKFSPWLALGCLSARYIAAQVAEYEQKRIANNSTYWLIFELLWRDYFRWICAKHGNQIFQPEGLQGVKIPWKQ
DHEAFELWRTGQTGIPFVDANMRELLYTGFMSNRGRQNVGSFLTKNLGIDWRWGAAWFESQLIDYDVCSNWGNWNYTAGV
GNDARGFRYFNIPKQAKDYDPQGDYVRHWLPELAALPGEQIHQPWRLSVKELGDRQIILGETYPKPMVDLQTSVQENERI
YQKALGLAPKSQSRKPRKNFKRNQDTPKARWRR

Specific function: May have a photoreceptor function. Binds DNA; probably functions as a transcriptional repressor [H]

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI4758072, Length=499, Percent_Identity=28.4569138276553, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI188536100, Length=499, Percent_Identity=27.6553106212425, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI188536103, Length=441, Percent_Identity=28.1179138321995, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1786926, Length=498, Percent_Identity=30.1204819277108, Blast_Score=179, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6324962, Length=522, Percent_Identity=28.1609195402299, Blast_Score=173, Evalue=6e-44,
Organism=Drosophila melanogaster, GI17137248, Length=510, Percent_Identity=27.6470588235294, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24585455, Length=510, Percent_Identity=27.6470588235294, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24648152, Length=526, Percent_Identity=25.8555133079848, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR014133
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 59459; Mature: 59328

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVNILLWYRRDLRLHDHQPLQEALAQQAQIFPFYCFDPRGFGSIGLDFPKTGAFRGQF
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHH
LREAIQDLRQNLRAKGSDLIVRQDIPEKLIPDLCQQWQIDVVYCHREVTAEETRVERKLR
HHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHH
QALQEQGVRVETFWGTTLHDPGDLPFGINEVPEVFTQFRKDVEKYAAIASPVPTPTQLPP
HHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LPETVAPGEIPSLQDLGLPEPTIDPRGVLPLKGGETAGMKRLQAYFWEGDRLKVYKETRN
CCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHC
GLLGADYSSKFSPWLALGCLSARYIAAQVAEYEQKRIANNSTYWLIFELLWRDYFRWICA
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KHGNQIFQPEGLQGVKIPWKQDHEAFELWRTGQTGIPFVDANMRELLYTGFMSNRGRQNV
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHCCCCHHHH
GSFLTKNLGIDWRWGAAWFESQLIDYDVCSNWGNWNYTAGVGNDARGFRYFNIPKQAKDY
HHHHHHHCCCCEECCHHHHHHHCCCHHHHCCCCCCCEECCCCCCCCCCEEECCCCHHCCC
DPQGDYVRHWLPELAALPGEQIHQPWRLSVKELGDRQIILGETYPKPMVDLQTSVQENER
CCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHH
IYQKALGLAPKSQSRKPRKNFKRNQDTPKARWRR
HHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHCCC
>Mature Secondary Structure 
SSVNILLWYRRDLRLHDHQPLQEALAQQAQIFPFYCFDPRGFGSIGLDFPKTGAFRGQF
CCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHH
LREAIQDLRQNLRAKGSDLIVRQDIPEKLIPDLCQQWQIDVVYCHREVTAEETRVERKLR
HHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHH
QALQEQGVRVETFWGTTLHDPGDLPFGINEVPEVFTQFRKDVEKYAAIASPVPTPTQLPP
HHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
LPETVAPGEIPSLQDLGLPEPTIDPRGVLPLKGGETAGMKRLQAYFWEGDRLKVYKETRN
CCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHC
GLLGADYSSKFSPWLALGCLSARYIAAQVAEYEQKRIANNSTYWLIFELLWRDYFRWICA
CCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KHGNQIFQPEGLQGVKIPWKQDHEAFELWRTGQTGIPFVDANMRELLYTGFMSNRGRQNV
HCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCHHHHHHHHHHCCCCHHHH
GSFLTKNLGIDWRWGAAWFESQLIDYDVCSNWGNWNYTAGVGNDARGFRYFNIPKQAKDY
HHHHHHHCCCCEECCHHHHHHHCCCHHHHCCCCCCCEECCCCCCCCCCEEECCCCHHCCC
DPQGDYVRHWLPELAALPGEQIHQPWRLSVKELGDRQIILGETYPKPMVDLQTSVQENER
CCCCHHHHHHHHHHHHCCCHHHCCCCCCHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHH
IYQKALGLAPKSQSRKPRKNFKRNQDTPKARWRR
HHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]