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The map label for this gene is sphS [H]

Identifier: 170078701

GI number: 170078701

Start: 2175838

End: 2177133

Strand: Direct

Name: sphS [H]

Synonym: SYNPCC7002_A2100

Alternate gene names: 170078701

Gene position: 2175838-2177133 (Clockwise)

Preceding gene: 170078697

Following gene: 170078702

Centisome position: 72.33

GC content: 49.92

Gene sequence:

>1296_bases
ATGATTTGGATTCCGATAATCATTGGCTGTGGCACAGCAGGCATTATTTGTTTCACTTGGCAACAGCGCCAAAAGCAACA
AGTCCGACAAATGCTTTACTCCTACGGTGAACATCCCAGTGACGAGCTTCTCTTTGCGCCTCTTTCTTCTCTCCGCCGGG
AACTAAAAAGCCTCTACGAAGAAAAACAAGAACTCCAGGCAGAACTAGACCAATGGTATCGGGTGATCGAAAATGCCCCC
TTTGGGTATCTGCGGGTTGACATGGATAATCAACTCATTTTGTGCAACCCAAAGGCCCGACAGCTCCTGTGCTTGAATCG
TTGGCAGCCAGGGCAAATTCGTCTCTTGCTGGAATTAGTCCGCTCCTATGAGTTAGACCAGCTCATCGAAACCACCCGCA
GCGCTCGCACCGCCCAAAGCCAGGAATGGGAATTTTTTCCGAGCCATGACGCCCAAACCCGTGCTGAAGGCCCCAACCAA
CCGATACACCTGCGGGGAAGCACTATCCCTTTAAGTCGTGGCGATGTGGCGGTTTTTTTAGAAAACCAAGAGCCGATTCA
CATCCTGAAACGTTCCCAAGAAAAATTACTGTCTGATCTGACCCACGAATTACGGACGCCCCTAACGGCGATGCGGCTCC
TCGCAGAAACCCTAGAACAGCGTCTAACAGGCTTGGAATTGAAATGGGCTGGTCAAATTCTTAAGGAAATTAATCGTTTG
TTTTACCTTGTCCAGGACTGGCTAGAACTGAGCCGACTAGAGTCCCACCCCCACCAGGTGCTGAGCTACCAAACCTTTGA
TCTAGTGCAGTTGATTAACGATGCTTGGGAAACCCTCACCCCCCTCGCCCAACAAAAACAAGTCACACGACAATATCAGG
GGCCAGACGCATTAAAAATGACCGCCGATGGCGATCGCCTCACCCAGGTTTTTCTCAATCTTTTTGACAATGGCATTAAA
TACAATCCCGTTGGGGAGGCCATTTTTGTTACTGTATCTCCCCCTGAAGTCGATTCAGACTTCGTTACCGTAGAAGTGAT
CGATCAAGGCGTTGGCTTTGATCCAGAAGACTTGCCATTTGTGTTTGAGCGCTTGTACCGGGGCGACCAAGCAAGAACAA
GACCGGGAGATAATACCGATTTGCGCCAGGGGAGTGGGCTAGGCTTAGCGATCGTGCAGCAAATTATTACTGCCCATGGC
GGCACAATTACCGCCCGCAATCACCCCAAATATCGAGGCGCTTGGCTAACCTTCACCCTACCGCTGCAACTGCCTAATGA
GGATCTTGATATTTAA

Upstream 100 bases:

>100_bases
TAATACCACCAATGGGAAAGTCCTGCCCCATTCTCAACGCCATCATCCTGAGTAACCCCTAGCAAATAACGGCGATCGCC
AAAAGCACCCGTATCAAAGC

Downstream 100 bases:

>100_bases
TTTTCTCCCACAGGATCGTGAATAGATAAACAACGATCAAGAAATAAATTTGCACTGGCATATGATCTTCCCCCACATAT
CTGAACTAGATGGTGTCTCT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIWIPIIIGCGTAGIICFTWQQRQKQQVRQMLYSYGEHPSDELLFAPLSSLRRELKSLYEEKQELQAELDQWYRVIENAP
FGYLRVDMDNQLILCNPKARQLLCLNRWQPGQIRLLLELVRSYELDQLIETTRSARTAQSQEWEFFPSHDAQTRAEGPNQ
PIHLRGSTIPLSRGDVAVFLENQEPIHILKRSQEKLLSDLTHELRTPLTAMRLLAETLEQRLTGLELKWAGQILKEINRL
FYLVQDWLELSRLESHPHQVLSYQTFDLVQLINDAWETLTPLAQQKQVTRQYQGPDALKMTADGDRLTQVFLNLFDNGIK
YNPVGEAIFVTVSPPEVDSDFVTVEVIDQGVGFDPEDLPFVFERLYRGDQARTRPGDNTDLRQGSGLGLAIVQQIITAHG
GTITARNHPKYRGAWLTFTLPLQLPNEDLDI

Sequences:

>Translated_431_residues
MIWIPIIIGCGTAGIICFTWQQRQKQQVRQMLYSYGEHPSDELLFAPLSSLRRELKSLYEEKQELQAELDQWYRVIENAP
FGYLRVDMDNQLILCNPKARQLLCLNRWQPGQIRLLLELVRSYELDQLIETTRSARTAQSQEWEFFPSHDAQTRAEGPNQ
PIHLRGSTIPLSRGDVAVFLENQEPIHILKRSQEKLLSDLTHELRTPLTAMRLLAETLEQRLTGLELKWAGQILKEINRL
FYLVQDWLELSRLESHPHQVLSYQTFDLVQLINDAWETLTPLAQQKQVTRQYQGPDALKMTADGDRLTQVFLNLFDNGIK
YNPVGEAIFVTVSPPEVDSDFVTVEVIDQGVGFDPEDLPFVFERLYRGDQARTRPGDNTDLRQGSGLGLAIVQQIITAHG
GTITARNHPKYRGAWLTFTLPLQLPNEDLDI
>Mature_431_residues
MIWIPIIIGCGTAGIICFTWQQRQKQQVRQMLYSYGEHPSDELLFAPLSSLRRELKSLYEEKQELQAELDQWYRVIENAP
FGYLRVDMDNQLILCNPKARQLLCLNRWQPGQIRLLLELVRSYELDQLIETTRSARTAQSQEWEFFPSHDAQTRAEGPNQ
PIHLRGSTIPLSRGDVAVFLENQEPIHILKRSQEKLLSDLTHELRTPLTAMRLLAETLEQRLTGLELKWAGQILKEINRL
FYLVQDWLELSRLESHPHQVLSYQTFDLVQLINDAWETLTPLAQQKQVTRQYQGPDALKMTADGDRLTQVFLNLFDNGIK
YNPVGEAIFVTVSPPEVDSDFVTVEVIDQGVGFDPEDLPFVFERLYRGDQARTRPGDNTDLRQGSGLGLAIVQQIITAHG
GTITARNHPKYRGAWLTFTLPLQLPNEDLDI

Specific function: Member of the two-component regulatory system sphR/sphS. Sensory kinase. Is involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to phos

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=373, Percent_Identity=26.0053619302949, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=30.9322033898305, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788393, Length=230, Percent_Identity=30.8695652173913, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786783, Length=231, Percent_Identity=31.6017316017316, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790346, Length=234, Percent_Identity=33.7606837606838, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790861, Length=215, Percent_Identity=32.5581395348837, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=238, Percent_Identity=29.8319327731092, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI48994928, Length=249, Percent_Identity=28.5140562248996, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1788713, Length=245, Percent_Identity=25.3061224489796, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI145693157, Length=240, Percent_Identity=27.5, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789149, Length=249, Percent_Identity=28.1124497991968, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788549, Length=362, Percent_Identity=25.6906077348066, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789403, Length=227, Percent_Identity=32.5991189427313, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1787894, Length=278, Percent_Identity=28.7769784172662, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49583; Mature: 49583

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWIPIIIGCGTAGIICFTWQQRQKQQVRQMLYSYGEHPSDELLFAPLSSLRRELKSLYE
CEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EKQELQAELDQWYRVIENAPFGYLRVDMDNQLILCNPKARQLLCLNRWQPGQIRLLLELV
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCEEEECCCCCHHHHHHHHHH
RSYELDQLIETTRSARTAQSQEWEFFPSHDAQTRAEGPNQPIHLRGSTIPLSRGDVAVFL
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEE
ENQEPIHILKRSQEKLLSDLTHELRTPLTAMRLLAETLEQRLTGLELKWAGQILKEINRL
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FYLVQDWLELSRLESHPHQVLSYQTFDLVQLINDAWETLTPLAQQKQVTRQYQGPDALKM
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
TADGDRLTQVFLNLFDNGIKYNPVGEAIFVTVSPPEVDSDFVTVEVIDQGVGFDPEDLPF
ECCHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCHHH
VFERLYRGDQARTRPGDNTDLRQGSGLGLAIVQQIITAHGGTITARNHPKYRGAWLTFTL
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEE
PLQLPNEDLDI
EEECCCCCCCC
>Mature Secondary Structure
MIWIPIIIGCGTAGIICFTWQQRQKQQVRQMLYSYGEHPSDELLFAPLSSLRRELKSLYE
CEEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
EKQELQAELDQWYRVIENAPFGYLRVDMDNQLILCNPKARQLLCLNRWQPGQIRLLLELV
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEECCCCCCEEEECCCCCHHHHHHHHHH
RSYELDQLIETTRSARTAQSQEWEFFPSHDAQTRAEGPNQPIHLRGSTIPLSRGDVAVFL
HHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEE
ENQEPIHILKRSQEKLLSDLTHELRTPLTAMRLLAETLEQRLTGLELKWAGQILKEINRL
CCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FYLVQDWLELSRLESHPHQVLSYQTFDLVQLINDAWETLTPLAQQKQVTRQYQGPDALKM
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
TADGDRLTQVFLNLFDNGIKYNPVGEAIFVTVSPPEVDSDFVTVEVIDQGVGFDPEDLPF
ECCHHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCCHHH
VFERLYRGDQARTRPGDNTDLRQGSGLGLAIVQQIITAHGGTITARNHPKYRGAWLTFTL
HHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEE
PLQLPNEDLDI
EEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497200 [H]