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The map label for this gene is purT [H]

Identifier: 170078634

GI number: 170078634

Start: 2111125

End: 2112312

Strand: Reverse

Name: purT [H]

Synonym: SYNPCC7002_A2030

Alternate gene names: 170078634

Gene position: 2112312-2111125 (Counterclockwise)

Preceding gene: 170078635

Following gene: 170078625

Centisome position: 70.22

GC content: 53.03

Gene sequence:

>1188_bases
ATGGCGATCGCATTACCGAAAAAAATCATGTTGTTGGGCTCTGGGGAACTGGGTAAAGAAGTGGCGATCGCCGCCCAACG
TTTAGGCAATACCGTGATCGCTGTGGATCGCTATGACAACGCCCCGGCAATGCAGGTGGCCGACCAACGGGAAGTCATCT
CTATGCTTGATGGGGTAGCCCTCGAAGCGGTGATCAAAAAACATCAACCGGATCTGATCGTGCCGGAAGTAGAAGCCATC
CGCACCGAAAAACTCATCGAATTAGAACAGCAAGGCTATACCGTTATCCCCACCGCCGCCGCCACCAACTTCACCATGAA
CCGCGATCGCATCCGGGATCTTGCCAGCGAAGAATTAGGCCTGCGTACTGCCAAATATGCCTATGCCACCAACCTCGCAG
AACTCAAAACCGTCGCCCAGGACATCGGCTTTCCCAACGTGATCAAGCCCGTAATGTCCTCCTCTGGTAAAGGGCAATCC
GTGGTACAAACTGCCGACGAATTAGAAGCCGCCTGGAATTACGCCATTGAGGGGGCCAGGGGTGACACCGCCAAAATTAT
CATCGAAGAATTTATCGATTTCGAGGTAGAAATCACCCTCCTCACCATCCGCCAATGGGCAGGAGAAACCCTCTTTTGTC
CGGCGATCGCCCACCGCCAAGAGCGGGGTGACTACCAAGAATCCTGGCAGCCCGTTGGTTTAACCGCAGCCCAAGTAAAA
GATGCCCAGGACATTGCCCGGAAAGTTACCGATAAACTCGGGGGCGCTGGCATTTTTGGGGTCGAGTTTTTTATCACCAA
AGATGAAGTAATTTTTTCGGAATTGTCTCCCCGGCCCCACGATACGGGCATGGTGACGCTCATTTCCCAAAACCTGAATG
AATTTGAACTCCATCTCCGGGCGATCTTGGGTCTACCGATTCCTACCATTGAATTGCTGAGTCCGGCGGCCAGTGCAGTG
ATTCTCGCTGAGAAAAATAGCGATCAAATTATGTTTACTGGTGTTGCAGCAGCCTTGGCTATCCCAGACACCGACCTCCG
TTTATTTGGTAAGCCCGATTCTCGTCCCTACCGTCGCATGGGGGTCGCCCTCGCCAAGGGGAAAGATGCTACCGAAGCTC
GTCAAAAGGCAACGACGGCAGCAGCCACAATCAAAATTACTGCAACAGCTCCTCACCCGAGCCATTGA

Upstream 100 bases:

>100_bases
TTTCCTCTCAGCCCGACAAAAACCCAAGGCCTGGCGGGGGCGCGTGTACAGCTAAAAACGGTATGATATCTCTGTTTTGT
TCTTGGCAATAGGTGAAAAA

Downstream 100 bases:

>100_bases
GGATTAAAAAAGATCAACAGGACCAAAGGAATCGACTTCTTCTAGTCCCTCGTTGAACGATAAATAAACGTAAGGAAAAA
GACGTGCTGGTGGGCAATGT

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAIALPKKIMLLGSGELGKEVAIAAQRLGNTVIAVDRYDNAPAMQVADQREVISMLDGVALEAVIKKHQPDLIVPEVEAI
RTEKLIELEQQGYTVIPTAAATNFTMNRDRIRDLASEELGLRTAKYAYATNLAELKTVAQDIGFPNVIKPVMSSSGKGQS
VVQTADELEAAWNYAIEGARGDTAKIIIEEFIDFEVEITLLTIRQWAGETLFCPAIAHRQERGDYQESWQPVGLTAAQVK
DAQDIARKVTDKLGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAILGLPIPTIELLSPAASAV
ILAEKNSDQIMFTGVAAALAIPDTDLRLFGKPDSRPYRRMGVALAKGKDATEARQKATTAAATIKITATAPHPSH

Sequences:

>Translated_395_residues
MAIALPKKIMLLGSGELGKEVAIAAQRLGNTVIAVDRYDNAPAMQVADQREVISMLDGVALEAVIKKHQPDLIVPEVEAI
RTEKLIELEQQGYTVIPTAAATNFTMNRDRIRDLASEELGLRTAKYAYATNLAELKTVAQDIGFPNVIKPVMSSSGKGQS
VVQTADELEAAWNYAIEGARGDTAKIIIEEFIDFEVEITLLTIRQWAGETLFCPAIAHRQERGDYQESWQPVGLTAAQVK
DAQDIARKVTDKLGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAILGLPIPTIELLSPAASAV
ILAEKNSDQIMFTGVAAALAIPDTDLRLFGKPDSRPYRRMGVALAKGKDATEARQKATTAAATIKITATAPHPSH
>Mature_394_residues
AIALPKKIMLLGSGELGKEVAIAAQRLGNTVIAVDRYDNAPAMQVADQREVISMLDGVALEAVIKKHQPDLIVPEVEAIR
TEKLIELEQQGYTVIPTAAATNFTMNRDRIRDLASEELGLRTAKYAYATNLAELKTVAQDIGFPNVIKPVMSSSGKGQSV
VQTADELEAAWNYAIEGARGDTAKIIIEEFIDFEVEITLLTIRQWAGETLFCPAIAHRQERGDYQESWQPVGLTAAQVKD
AQDIARKVTDKLGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLRAILGLPIPTIELLSPAASAVI
LAEKNSDQIMFTGVAAALAIPDTDLRLFGKPDSRPYRRMGVALAKGKDATEARQKATTAAATIKITATAPHPSH

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=379, Percent_Identity=53.2981530343008, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1786733, Length=360, Percent_Identity=26.1111111111111, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324702, Length=367, Percent_Identity=23.7057220708447, Blast_Score=106, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42881; Mature: 42749

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIALPKKIMLLGSGELGKEVAIAAQRLGNTVIAVDRYDNAPAMQVADQREVISMLDGVA
CCCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LEAVIKKHQPDLIVPEVEAIRTEKLIELEQQGYTVIPTAAATNFTMNRDRIRDLASEELG
HHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCEECCHHHHHHHHHHHHC
LRTAKYAYATNLAELKTVAQDIGFPNVIKPVMSSSGKGQSVVQTADELEAAWNYAIEGAR
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCC
GDTAKIIIEEFIDFEVEITLLTIRQWAGETLFCPAIAHRQERGDYQESWQPVGLTAAQVK
CCHHHHHHHHHCCCEEEEEEEEEEHHCCCEEECHHHHHHHHCCCHHHCCCCCCCCHHHHH
DAQDIARKVTDKLGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLR
HHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHH
AILGLPIPTIELLSPAASAVILAEKNSDQIMFTGVAAALAIPDTDLRLFGKPDSRPYRRM
HHHCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEEEHEEECCCCCEEEECCCCCCHHHHH
GVALAKGKDATEARQKATTAAATIKITATAPHPSH
CCHHCCCCCHHHHHHHHHHEEEEEEEEEECCCCCC
>Mature Secondary Structure 
AIALPKKIMLLGSGELGKEVAIAAQRLGNTVIAVDRYDNAPAMQVADQREVISMLDGVA
CCCCCCEEEEEECCCCCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LEAVIKKHQPDLIVPEVEAIRTEKLIELEQQGYTVIPTAAATNFTMNRDRIRDLASEELG
HHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCEECCHHHHHHHHHHHHC
LRTAKYAYATNLAELKTVAQDIGFPNVIKPVMSSSGKGQSVVQTADELEAAWNYAIEGAR
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHEEECCC
GDTAKIIIEEFIDFEVEITLLTIRQWAGETLFCPAIAHRQERGDYQESWQPVGLTAAQVK
CCHHHHHHHHHCCCEEEEEEEEEEHHCCCEEECHHHHHHHHCCCHHHCCCCCCCCHHHHH
DAQDIARKVTDKLGGAGIFGVEFFITKDEVIFSELSPRPHDTGMVTLISQNLNEFELHLR
HHHHHHHHHHHHHCCCEEEEEEEEEECCHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHH
AILGLPIPTIELLSPAASAVILAEKNSDQIMFTGVAAALAIPDTDLRLFGKPDSRPYRRM
HHHCCCCCHHHHHCCCCCEEEEEECCCCEEEEEEEEHEEECCCCCEEEECCCCCCHHHHH
GVALAKGKDATEARQKATTAAATIKITATAPHPSH
CCHHCCCCCHHHHHHHHHHEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA