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The map label for this gene is miaB

Identifier: 170078520

GI number: 170078520

Start: 1998437

End: 1999792

Strand: Reverse

Name: miaB

Synonym: SYNPCC7002_A1915

Alternate gene names: 170078520

Gene position: 1999792-1998437 (Counterclockwise)

Preceding gene: 170078530

Following gene: 170078519

Centisome position: 66.48

GC content: 51.11

Gene sequence:

>1356_bases
ATGACTAAGCCTCGTCAATATCACATTACCACTTTCGGTTGTCAGATGAACAAGGCCGACTCCGAACGTATGGCGGGCAT
CCTCGAAGAGATGGGCTATCACTTCACCGAAGATCCCTATGCTGCAGATCTCGTCCTCTACAATACCTGCACGATTCGGG
ATAATGCGGAACAAAAAGTTTACTCTTACCTCGGTCGTCAAGCTAAGCGGAAACAAGAAAAACCCGACCTCACCCTGATC
GTTGCCGGTTGCGTCGCCCAACAGGAAGGAGAAAGTCTCCTGCGCCGTGTCCCAGAACTAGATCTGATCATGGGGCCTCA
ACATGCCAACCGTCTCCAGGATTTGTTAGAGCAAGTCGAAGGGGGAAGTCAGGTGGTGGCAACGGAGCCAATCCATATCG
TTGAAGATATTACGAAACCCCGGCGAGACAGCACGGTCACGGCCTGGGTCAATGTGATCTATGGCTGCAATGAGCACTGT
ACCTATTGTGTTGTTCCTGGCGTGCGGGGCACGGAACAATCGCGCTATCCAGAAGCGATCCGGGCAGAAATGGAAGAACT
AGGGCGACAAGGGTTTAAGGAAGTCACGCTCCTCGGTCAAAACATCGATGCCTATGGCCGGGATTTACCGGGGACAACAC
CGGAGGGCAGAAATAAATACACCCTCACGGATCTTTTGTATTACGTTCATGATGTGCCGGGCATTGAGCGGATTCGCTTT
GCCACCAGTCACCCCCGCTACTTTACGGAACGCTTGATTAAGGCTTGCCAGGAGTTACCCAAGGTTTGTGAGCATTTTCA
TATTCCTTTTCAGTCTGGGGACAATGAGGTGCTTAAGGCGATGCGACGCGGTTATACCCACGAAAAATATCGCCGCATTA
TCAATACCATTCGGAGTTATATGCCCGATGCTTCCATTAGTGCGGATGCGATTGTGGCGTTCCCCGGAGAAACGGAAGAG
CAGTTTGAAAACACCCTCAAATTGGTGGATGAGATTGGTTTTGATCAATTAAATACGGCGGCCTATTCTCCCCGACCCGG
CACCCCCGCCGCCACTTGGGATAATCAATTGTCAGAGGAAGTAAAAGGCGATCGCCTCCAACGTTTGAATCATTTGGTCG
CCCAAAAAGCAGCAGAACGCTCCCAACGCTATGCCGGACGGATCGAAGAAGTGCTCGTTGAAGATCAAAATCCGAAAAAT
CCAAGTCAGGTTATGGGACGGACCCGGGGCAACCGCCTCACCTTTTTTGAGGGAGAGATTAACGAACTCAAAGGAAAAAT
TGTTGCGGTCAAAATCACCGAAGCACGGGCCTTTAGCCTAACCGGAGAAGCCGTTGAAGCCCTCGTAACTGCTTAG

Upstream 100 bases:

>100_bases
AGGTTTTGGCGGATTCACATCCCCATCTCCCACAAGCGAAACCTAAATTGGTAGAATAAAAAGCTGCTAATTTGCCTAGC
CCATTATTTTTGAAACGACC

Downstream 100 bases:

>100_bases
ATACCATGACCACTCAACCTTGGCTCACCCCAGCGGCGATCGCCCAAGTGCAGCTTATTTTGGATAGTTATCATCATTGG
TTTGGGGCGGATTTGATTGA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTKPRQYHITTFGCQMNKADSERMAGILEEMGYHFTEDPYAADLVLYNTCTIRDNAEQKVYSYLGRQAKRKQEKPDLTLI
VAGCVAQQEGESLLRRVPELDLIMGPQHANRLQDLLEQVEGGSQVVATEPIHIVEDITKPRRDSTVTAWVNVIYGCNEHC
TYCVVPGVRGTEQSRYPEAIRAEMEELGRQGFKEVTLLGQNIDAYGRDLPGTTPEGRNKYTLTDLLYYVHDVPGIERIRF
ATSHPRYFTERLIKACQELPKVCEHFHIPFQSGDNEVLKAMRRGYTHEKYRRIINTIRSYMPDASISADAIVAFPGETEE
QFENTLKLVDEIGFDQLNTAAYSPRPGTPAATWDNQLSEEVKGDRLQRLNHLVAQKAAERSQRYAGRIEEVLVEDQNPKN
PSQVMGRTRGNRLTFFEGEINELKGKIVAVKITEARAFSLTGEAVEALVTA

Sequences:

>Translated_451_residues
MTKPRQYHITTFGCQMNKADSERMAGILEEMGYHFTEDPYAADLVLYNTCTIRDNAEQKVYSYLGRQAKRKQEKPDLTLI
VAGCVAQQEGESLLRRVPELDLIMGPQHANRLQDLLEQVEGGSQVVATEPIHIVEDITKPRRDSTVTAWVNVIYGCNEHC
TYCVVPGVRGTEQSRYPEAIRAEMEELGRQGFKEVTLLGQNIDAYGRDLPGTTPEGRNKYTLTDLLYYVHDVPGIERIRF
ATSHPRYFTERLIKACQELPKVCEHFHIPFQSGDNEVLKAMRRGYTHEKYRRIINTIRSYMPDASISADAIVAFPGETEE
QFENTLKLVDEIGFDQLNTAAYSPRPGTPAATWDNQLSEEVKGDRLQRLNHLVAQKAAERSQRYAGRIEEVLVEDQNPKN
PSQVMGRTRGNRLTFFEGEINELKGKIVAVKITEARAFSLTGEAVEALVTA
>Mature_450_residues
TKPRQYHITTFGCQMNKADSERMAGILEEMGYHFTEDPYAADLVLYNTCTIRDNAEQKVYSYLGRQAKRKQEKPDLTLIV
AGCVAQQEGESLLRRVPELDLIMGPQHANRLQDLLEQVEGGSQVVATEPIHIVEDITKPRRDSTVTAWVNVIYGCNEHCT
YCVVPGVRGTEQSRYPEAIRAEMEELGRQGFKEVTLLGQNIDAYGRDLPGTTPEGRNKYTLTDLLYYVHDVPGIERIRFA
TSHPRYFTERLIKACQELPKVCEHFHIPFQSGDNEVLKAMRRGYTHEKYRRIINTIRSYMPDASISADAIVAFPGETEEQ
FENTLKLVDEIGFDQLNTAAYSPRPGTPAATWDNQLSEEVKGDRLQRLNHLVAQKAAERSQRYAGRIEEVLVEDQNPKNP
SQVMGRTRGNRLTFFEGEINELKGKIVAVKITEARAFSLTGEAVEALVTA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=32.6403326403326, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI28872782, Length=481, Percent_Identity=32.4324324324324, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI93277076, Length=452, Percent_Identity=30.0884955752212, Blast_Score=170, Evalue=3e-42,
Organism=Escherichia coli, GI1786882, Length=450, Percent_Identity=41.5555555555556, Blast_Score=333, Evalue=1e-92,
Organism=Escherichia coli, GI1787057, Length=450, Percent_Identity=27.5555555555556, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=32.9718004338395, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=279, Percent_Identity=30.8243727598566, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=32.5678496868476, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=28.9760348583878, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_SYNP2 (B1XQK7)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001735158.1
- ProteinModelPortal:   B1XQK7
- SMR:   B1XQK7
- GeneID:   6055855
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1915
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14333
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 51060; Mature: 50929

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPRQYHITTFGCQMNKADSERMAGILEEMGYHFTEDPYAADLVLYNTCTIRDNAEQKV
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCHHHHH
YSYLGRQAKRKQEKPDLTLIVAGCVAQQEGESLLRRVPELDLIMGPQHANRLQDLLEQVE
HHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHC
GGSQVVATEPIHIVEDITKPRRDSTVTAWVNVIYGCNEHCTYCVVPGVRGTEQSRYPEAI
CCCEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHH
RAEMEELGRQGFKEVTLLGQNIDAYGRDLPGTTPEGRNKYTLTDLLYYVHDVPGIERIRF
HHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHEE
ATSHPRYFTERLIKACQELPKVCEHFHIPFQSGDNEVLKAMRRGYTHEKYRRIINTIRSY
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
MPDASISADAIVAFPGETEEQFENTLKLVDEIGFDQLNTAAYSPRPGTPAATWDNQLSEE
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHHH
VKGDRLQRLNHLVAQKAAERSQRYAGRIEEVLVEDQNPKNPSQVMGRTRGNRLTFFEGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCH
NELKGKIVAVKITEARAFSLTGEAVEALVTA
HHCCCEEEEEEEECCCEEECCHHHHHHHHCC
>Mature Secondary Structure 
TKPRQYHITTFGCQMNKADSERMAGILEEMGYHFTEDPYAADLVLYNTCTIRDNAEQKV
CCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCHHHHH
YSYLGRQAKRKQEKPDLTLIVAGCVAQQEGESLLRRVPELDLIMGPQHANRLQDLLEQVE
HHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHC
GGSQVVATEPIHIVEDITKPRRDSTVTAWVNVIYGCNEHCTYCVVPGVRGTEQSRYPEAI
CCCEEEEECCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCHHHH
RAEMEELGRQGFKEVTLLGQNIDAYGRDLPGTTPEGRNKYTLTDLLYYVHDVPGIERIRF
HHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHEE
ATSHPRYFTERLIKACQELPKVCEHFHIPFQSGDNEVLKAMRRGYTHEKYRRIINTIRSY
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
MPDASISADAIVAFPGETEEQFENTLKLVDEIGFDQLNTAAYSPRPGTPAATWDNQLSEE
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHHHHH
VKGDRLQRLNHLVAQKAAERSQRYAGRIEEVLVEDQNPKNPSQVMGRTRGNRLTFFEGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCH
NELKGKIVAVKITEARAFSLTGEAVEALVTA
HHCCCEEEEEEEECCCEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA