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The map label for this gene is proB

Identifier: 170078388

GI number: 170078388

Start: 1867289

End: 1868413

Strand: Reverse

Name: proB

Synonym: SYNPCC7002_A1782

Alternate gene names: 170078388

Gene position: 1868413-1867289 (Counterclockwise)

Preceding gene: 170078391

Following gene: 170078387

Centisome position: 62.11

GC content: 54.4

Gene sequence:

>1125_bases
ATGCCCCAAACCATCGTCGTTAAAATTGGCACCTCTAGCCTCACAGATCCCAAGACAGGCATGTTGGCTTTGGCAACTAT
CGGTACCCTCGTGGAAACCTTGACCCGCCTCAAACAAAAGGGCCACCGGGTGATCCTCGTCTCCTCTGGGGCCGTGGGCG
TGGGCTGTGGGGTGTTGAAACGTTCAGAACGCCCGAAAAAAATTGCCGAAAAACAGGCGATCGCCGCCGTGGGTCAGGGG
CGCTTAATCCGGCTTTATGATGATCTATTTACCAGTCTGCAACAGGCGATCGCCCAGGTATTACTCACCCGTCGAGACCT
CGTGGATCGCACTTCCTACCTGACCGTCCAGGAAGCCCTCAACGCGATGTTGGATCTCAGCGTGATCCCGATTGTGAACG
AAAACGATACCGTTGCCGTCGATGAACTGAAATTCGGCGATAACGATACCCTCTCTGCCCTGGTCGCCAGCTTGGTGAAT
GCCGACTGGTTGGTGCTACTCACCGATGTGGATCGCCTCTATTCCGCTGACCCCAGACAAAATCCTGACGCCCGCCCTAT
TCCCCTGGTCAGTTCCAGCGAACTGTTTAACCTCCAAGTCGATGCTGGATCTAGCGGCTCCCAATGGGGGACAGGGGGCA
TGCAAACAAAGCTCACTGCTGCTCGCATTGCCACCAGCGCTGGCGTACGCACGGTGATTACCCAGGGCAAAACCCCCCAG
AATCTCCTGAAAATTGTTGCTGGGGAAGATATCGGCACCCATTTTGAAGCCCAACCCCAGGCCGATAATGCCCGTAAACG
TTGGATTGCCTATGGCCTTGTACCCCAGGGAATTCTCACCCTTGACGATGGCGCAACCCAGGCTCTCCTGAAAAATGGTC
GTTCTCTCCTTGCGGCAGGTATTGTTGCCGTGGAAGGAGAATTTACCATTAACGACCCCGTAGAACTGAGAACCCAAACA
GGTCAGGCGATCGCCAGAGGGTTAGTAAACTACAACCACCAAGATATTGAAAAAATTCAAGGGCAACATTCGACCCAAAT
TCGCCAAATTCTCGGCTACGGCAGCGCCGACACCGTGATTCACCGAGATAACTTGGCCCTGCTGATCGCCGCTGAAGGGA
TATGA

Upstream 100 bases:

>100_bases
CGGGAGACGGAAAGGATGCTGTGGACGTGGGGTAAGATTTTCGGCTCAAGGGAATTTTTGCTGAAATAATAGAAAAATTC
ACCTAGCCTCACAGTCTTCT

Downstream 100 bases:

>100_bases
GAAAATAATCTATGGCCTAAATTTCCTTAGACCTGCGCTGACCACTGCTCACCTTTCCATGAATCTAAAAACGAACCTCC
TTGAAACCATTGCCGGAAAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MPQTIVVKIGTSSLTDPKTGMLALATIGTLVETLTRLKQKGHRVILVSSGAVGVGCGVLKRSERPKKIAEKQAIAAVGQG
RLIRLYDDLFTSLQQAIAQVLLTRRDLVDRTSYLTVQEALNAMLDLSVIPIVNENDTVAVDELKFGDNDTLSALVASLVN
ADWLVLLTDVDRLYSADPRQNPDARPIPLVSSSELFNLQVDAGSSGSQWGTGGMQTKLTAARIATSAGVRTVITQGKTPQ
NLLKIVAGEDIGTHFEAQPQADNARKRWIAYGLVPQGILTLDDGATQALLKNGRSLLAAGIVAVEGEFTINDPVELRTQT
GQAIARGLVNYNHQDIEKIQGQHSTQIRQILGYGSADTVIHRDNLALLIAAEGI

Sequences:

>Translated_374_residues
MPQTIVVKIGTSSLTDPKTGMLALATIGTLVETLTRLKQKGHRVILVSSGAVGVGCGVLKRSERPKKIAEKQAIAAVGQG
RLIRLYDDLFTSLQQAIAQVLLTRRDLVDRTSYLTVQEALNAMLDLSVIPIVNENDTVAVDELKFGDNDTLSALVASLVN
ADWLVLLTDVDRLYSADPRQNPDARPIPLVSSSELFNLQVDAGSSGSQWGTGGMQTKLTAARIATSAGVRTVITQGKTPQ
NLLKIVAGEDIGTHFEAQPQADNARKRWIAYGLVPQGILTLDDGATQALLKNGRSLLAAGIVAVEGEFTINDPVELRTQT
GQAIARGLVNYNHQDIEKIQGQHSTQIRQILGYGSADTVIHRDNLALLIAAEGI
>Mature_373_residues
PQTIVVKIGTSSLTDPKTGMLALATIGTLVETLTRLKQKGHRVILVSSGAVGVGCGVLKRSERPKKIAEKQAIAAVGQGR
LIRLYDDLFTSLQQAIAQVLLTRRDLVDRTSYLTVQEALNAMLDLSVIPIVNENDTVAVDELKFGDNDTLSALVASLVNA
DWLVLLTDVDRLYSADPRQNPDARPIPLVSSSELFNLQVDAGSSGSQWGTGGMQTKLTAARIATSAGVRTVITQGKTPQN
LLKIVAGEDIGTHFEAQPQADNARKRWIAYGLVPQGILTLDDGATQALLKNGRSLLAAGIVAVEGEFTINDPVELRTQTG
QAIARGLVNYNHQDIEKIQGQHSTQIRQILGYGSADTVIHRDNLALLIAAEGI

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=292, Percent_Identity=30.4794520547945, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI62912457, Length=290, Percent_Identity=30.3448275862069, Blast_Score=112, Evalue=7e-25,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=44.6280991735537, Blast_Score=280, Evalue=1e-76,
Organism=Caenorhabditis elegans, GI17569969, Length=274, Percent_Identity=35.4014598540146, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17569967, Length=274, Percent_Identity=35.4014598540146, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=42.5480769230769, Blast_Score=305, Evalue=7e-84,
Organism=Saccharomyces cerevisiae, GI6321822, Length=419, Percent_Identity=38.6634844868735, Blast_Score=266, Evalue=3e-72,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=31.2714776632302, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_SYNP2 (B1XPQ6)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001735026.1
- ProteinModelPortal:   B1XPQ6
- SMR:   B1XPQ6
- GeneID:   6055817
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1782
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40066; Mature: 39935

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTIVVKIGTSSLTDPKTGMLALATIGTLVETLTRLKQKGHRVILVSSGAVGVGCGVLK
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
RSERPKKIAEKQAIAAVGQGRLIRLYDDLFTSLQQAIAQVLLTRRDLVDRTSYLTVQEAL
CCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHH
NAMLDLSVIPIVNENDTVAVDELKFGDNDTLSALVASLVNADWLVLLTDVDRLYSADPRQ
HHHHCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCC
NPDARPIPLVSSSELFNLQVDAGSSGSQWGTGGMQTKLTAARIATSAGVRTVITQGKTPQ
CCCCCCCCEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHH
NLLKIVAGEDIGTHFEAQPQADNARKRWIAYGLVPQGILTLDDGATQALLKNGRSLLAAG
HHHHHHCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCEEEECCCHHHHHHHCCHHEEECC
IVAVEGEFTINDPVELRTQTGQAIARGLVNYNHQDIEKIQGQHSTQIRQILGYGSADTVI
EEEECCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEE
HRDNLALLIAAEGI
EECCEEEEEEECCC
>Mature Secondary Structure 
PQTIVVKIGTSSLTDPKTGMLALATIGTLVETLTRLKQKGHRVILVSSGAVGVGCGVLK
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
RSERPKKIAEKQAIAAVGQGRLIRLYDDLFTSLQQAIAQVLLTRRDLVDRTSYLTVQEAL
CCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHH
NAMLDLSVIPIVNENDTVAVDELKFGDNDTLSALVASLVNADWLVLLTDVDRLYSADPRQ
HHHHCCEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCC
NPDARPIPLVSSSELFNLQVDAGSSGSQWGTGGMQTKLTAARIATSAGVRTVITQGKTPQ
CCCCCCCCEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHH
NLLKIVAGEDIGTHFEAQPQADNARKRWIAYGLVPQGILTLDDGATQALLKNGRSLLAAG
HHHHHHCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCEEEECCCHHHHHHHCCHHEEECC
IVAVEGEFTINDPVELRTQTGQAIARGLVNYNHQDIEKIQGQHSTQIRQILGYGSADTVI
EEEECCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCEE
HRDNLALLIAAEGI
EECCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA