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The map label for this gene is lipA [H]

Identifier: 170078186

GI number: 170078186

Start: 1660469

End: 1661371

Strand: Direct

Name: lipA [H]

Synonym: SYNPCC7002_A1577

Alternate gene names: 170078186

Gene position: 1660469-1661371 (Clockwise)

Preceding gene: 170078182

Following gene: 170078187

Centisome position: 55.2

GC content: 53.05

Gene sequence:

>903_bases
ATGGCTTCTTCTGCGTCTCACTCCTCCAGCGCCCCGATTTTGCCCATGCCTAGTTGGGTCAAAGCTCCCCTTGGTAAAGC
GAGTGAGATCTCGACAGTCCAGCGCATCATTAAACAACGGGGCATTCATACCATCTGTGAGGAAGGGAAATGTCCGAATC
GAGGTGAGTGCTACGCCAATAGAACTGCAACCTTTTTATTGATGGGGCAAGTTTGCACCAGGGCCTGTGCCTTCTGTCAG
GTTGATAAAGGCCAAATTCCCCTCCCTCTTGACCCGGAGGAACCCCAAAAAGTGGCGGAGTCAGTGCGACTGTTGGGACT
GAGTTATGTGGTGTTGACATCGGTGGCGCGGGACGATCTTGCCGATGGGGGAGCAGGCTGGTTTGTGGCTGTGATGGAAG
CGATCCGGGCGGAAAATCCAGGGACACAAATTGAAGTCCTGACTCCGGATTTTTGGAGTGGGAAAGGGGCAGAGGTGGCC
CAGGAGGAACGGGTAAAAACAGTTGTGGCAGCGCAACCAGCTTGTTATAACCACAACATTGAAACAGTAGAAAGGCTCCA
GGGTCCTGTGCGTCGGGGGGCAAAGTATGAGCGATCGCTCCGGGTTTTGGAATTGGTAAAAGCGTACAATCCTGAGATAC
CGACGAAATCAGGCTTAATGCTCGGCCATGGAGAAACGAAAACAGAAATTATCCAGGCGTTACAAGATTTGCTGGCCGTG
GGCTGCGATCGCCTCACCCTAGGCCAATATATGCGCCCCTCCCTCGCCCATTTACCCGTCCAGAAATATTGGACACCAGC
GGAATTTGAACACCTAGGGGCGATCGCCAAAGAAATGGGCTTTTCCCATGTGCGTTCAGGGCCTCTGGTGCGTAGTTCCT
ACCATGCTGGCAAATCGATCTAA

Upstream 100 bases:

>100_bases
TCGATTAGCCTCGTGCCTAGGGATGTTGGGTTAACTTGATTTTCAGTAAACTAATTCTAAAAATCCCAGAACTTCCCTCA
ACAGCGTTTTTATAAAAATT

Downstream 100 bases:

>100_bases
GAGCGCCCTTGAGTATTGCGATTTATGAAAGAGTATTACTAGATTATGTCGTTTTGGGGCAATGCTACCGATGGAGTTCC
CTAAGCTTTTAAGCTTAAAA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MASSASHSSSAPILPMPSWVKAPLGKASEISTVQRIIKQRGIHTICEEGKCPNRGECYANRTATFLLMGQVCTRACAFCQ
VDKGQIPLPLDPEEPQKVAESVRLLGLSYVVLTSVARDDLADGGAGWFVAVMEAIRAENPGTQIEVLTPDFWSGKGAEVA
QEERVKTVVAAQPACYNHNIETVERLQGPVRRGAKYERSLRVLELVKAYNPEIPTKSGLMLGHGETKTEIIQALQDLLAV
GCDRLTLGQYMRPSLAHLPVQKYWTPAEFEHLGAIAKEMGFSHVRSGPLVRSSYHAGKSI

Sequences:

>Translated_300_residues
MASSASHSSSAPILPMPSWVKAPLGKASEISTVQRIIKQRGIHTICEEGKCPNRGECYANRTATFLLMGQVCTRACAFCQ
VDKGQIPLPLDPEEPQKVAESVRLLGLSYVVLTSVARDDLADGGAGWFVAVMEAIRAENPGTQIEVLTPDFWSGKGAEVA
QEERVKTVVAAQPACYNHNIETVERLQGPVRRGAKYERSLRVLELVKAYNPEIPTKSGLMLGHGETKTEIIQALQDLLAV
GCDRLTLGQYMRPSLAHLPVQKYWTPAEFEHLGAIAKEMGFSHVRSGPLVRSSYHAGKSI
>Mature_299_residues
ASSASHSSSAPILPMPSWVKAPLGKASEISTVQRIIKQRGIHTICEEGKCPNRGECYANRTATFLLMGQVCTRACAFCQV
DKGQIPLPLDPEEPQKVAESVRLLGLSYVVLTSVARDDLADGGAGWFVAVMEAIRAENPGTQIEVLTPDFWSGKGAEVAQ
EERVKTVVAAQPACYNHNIETVERLQGPVRRGAKYERSLRVLELVKAYNPEIPTKSGLMLGHGETKTEIIQALQDLLAVG
CDRLTLGQYMRPSLAHLPVQKYWTPAEFEHLGAIAKEMGFSHVRSGPLVRSSYHAGKSI

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=291, Percent_Identity=40.5498281786942, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI37577164, Length=254, Percent_Identity=39.3700787401575, Blast_Score=190, Evalue=1e-48,
Organism=Escherichia coli, GI1786846, Length=285, Percent_Identity=46.3157894736842, Blast_Score=264, Evalue=5e-72,
Organism=Caenorhabditis elegans, GI32564533, Length=294, Percent_Identity=42.8571428571429, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=42.6116838487972, Blast_Score=239, Evalue=3e-64,
Organism=Drosophila melanogaster, GI221513272, Length=291, Percent_Identity=43.298969072165, Blast_Score=234, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 32719; Mature: 32588

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSASHSSSAPILPMPSWVKAPLGKASEISTVQRIIKQRGIHTICEEGKCPNRGECYAN
CCCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHC
RTATFLLMGQVCTRACAFCQVDKGQIPLPLDPEEPQKVAESVRLLGLSYVVLTSVARDDL
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ADGGAGWFVAVMEAIRAENPGTQIEVLTPDFWSGKGAEVAQEERVKTVVAAQPACYNHNI
CCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCH
ETVERLQGPVRRGAKYERSLRVLELVKAYNPEIPTKSGLMLGHGETKTEIIQALQDLLAV
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
GCDRLTLGQYMRPSLAHLPVQKYWTPAEFEHLGAIAKEMGFSHVRSGPLVRSSYHAGKSI
CCCHHHHHHHHCCHHHHCCHHHCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSASHSSSAPILPMPSWVKAPLGKASEISTVQRIIKQRGIHTICEEGKCPNRGECYAN
CCCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHC
RTATFLLMGQVCTRACAFCQVDKGQIPLPLDPEEPQKVAESVRLLGLSYVVLTSVARDDL
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ADGGAGWFVAVMEAIRAENPGTQIEVLTPDFWSGKGAEVAQEERVKTVVAAQPACYNHNI
CCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCH
ETVERLQGPVRRGAKYERSLRVLELVKAYNPEIPTKSGLMLGHGETKTEIIQALQDLLAV
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHH
GCDRLTLGQYMRPSLAHLPVQKYWTPAEFEHLGAIAKEMGFSHVRSGPLVRSSYHAGKSI
CCCHHHHHHHHCCHHHHCCHHHCCCCHHHHHHHHHHHHHCHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]