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The map label for this gene is menD [H]

Identifier: 170078140

GI number: 170078140

Start: 1614102

End: 1615841

Strand: Direct

Name: menD [H]

Synonym: SYNPCC7002_A1531

Alternate gene names: 170078140

Gene position: 1614102-1615841 (Clockwise)

Preceding gene: 170078138

Following gene: 170078146

Centisome position: 53.66

GC content: 48.74

Gene sequence:

>1740_bases
ATGGGTCTAGATTTTAGTAATCTTAATAGTCTCTGGGCGGGTGTGATGGTAGAAACCCTCGCGCGCCTAGGTTTGCAATC
GGCAGTGATTTGTCCTGGATCCCGCTCTACGCCCCTCACCGTTGCCCTGGCCCGTCACCCCCAGATTAAAACGATCCCGA
TTTTAGATGAACGCTCGGCGGCGTTTTTTGCCCTGGGTTTAGCGAAAAGTTCTGGTTTACCGACGGTGCTGGTCTGTACG
TCGGGGACAGCGGGGGCCAATTTCTATCCAGCGGTGATCGAAGCCTACCAAAGTGCGGTGCCTCTGATTATCTTCACAGG
CGATCGCCCCCCGGAATTGCGGGATTGTCATTCGGGGCAAACCATTGACCAGATCAAGTTATTTGGAAATTTTGCCCAGT
GGTTTAGTGAAATGGCGACGCCCCTGGGCACCGTTGAAATGTTGCGCTATGCCCGACAAACCATGGTGCAAGCTTGGCGG
CGATCGCAGTTTCCCCAGAAAGGAGTCGTGCATCTCAATTGTCCGTTTCGGGATCCCCTTGCCCCCCTTGCCGATGGCTC
AGTGACGCACCTCAAAGATGTGCTCAAGGCGGATTTTTTTGCCGGGATTCAGCCCCTGGAAAAGGCAGAATTTTCTGCGG
ATATTTTGCCCACGGAAGCTTGGAAAAATTATGCCCGGGGGCTAATTATTGCTGGGGTGAATCAGCCCGATGATCCCGAT
GTATATTGTCAGGCGATCGCCCAGTTGTCGGGTTATTTGAGTTTTCCGGTTTTAACCGAAGCCCTCTCCCCCGTGCGGAA
TTATGCCCATTATTTTCCCCAGGGTTTAGTCATCACCTACGATTTTATTTTGCGGGATCAAAACCTAGCAGAAACCCTCA
AACCAGAAATTGTGATTCAAATTGGCGCTTTACCCACCAGTAAAATTTTGCGAGCCTGGCTCGAAGCACAGCAGATTCAA
ACCTATATCTTGAGCGAGCGGGCCGATAATTTTGACCCCCTCCATCGTCCCCACCAATATCTCGCCGTTAATCTTCGCAC
CATGAAGTATTTGGGGATCTTTCCCGACAACGAAAAAGGTTTGCCCTACTTGGATCTATGGCAGACCCAAGATGCAAAAA
TTCGCGCCAAATTAGCCGCAGTTTTCCAGGCAGAACAAAATCTCAACGAAGCGGCGATCGCCTACTGTTTAGCCAGAGCG
TTACCAGAGGAAACAGCTATTTTCTTTGCCAATAGCATGGTGGTGCGCTATGCCGAATTTTTCTGGCAGTTGAATGATCA
ACAAATTTTGCCCTACTTCAATCGGGGGGCCAATGGCATTGATGGTACCCTATCAACGGCTTTGGGCATTGCAGAGAATC
ATTCGCCTGCTGTGTTACTGACGGGGGATCTCGCTCTCCTCCACGATATCAATGGCTTTTTGACATTATCAAAATTTACT
GGCTCCCTGACGATTATTGTGATCAATAACCAAGGCGGCGGCATCTTTGAAATGTTACCCATCGCCGAAGAAAAAGATAT
TTTTGAGGATTATTTTGCGACGCCCCAAACCGTTGAATTTTCTAAAGTATGCGATTTATATGGCATTGAATATCAGGCGA
TCCAAACCAAAGCTGATCTGATGCAAACCTGTGCTATCTTGCCACCCAGGGGAGTACGGCTATTGGAAGTCAAAACAAAC
CGTAACCAAGCGATGACTTGGCTGAAAGATTTATTCCAAGGTTTTCAAAGTCATTTTTAA

Upstream 100 bases:

>100_bases
GGGGAAAAACTAGAGGGTAAAGCAATCATGCCAAAAGACTGAAAATCAGTGCTTTAATTGTACCCTGGTTGATTTGGGAG
CATAGTTTAGCACGGAAGGT

Downstream 100 bases:

>100_bases
ATGGCGTGTTTTTTCACTAAATAGGTGATTTTTTCGGCTTCATCGGTCGTTAATTCCGGAGCTTCTTTAATGCTTTGGCG
GTACATCTTGAGATATTCCC

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 579; Mature: 578

Protein sequence:

>579_residues
MGLDFSNLNSLWAGVMVETLARLGLQSAVICPGSRSTPLTVALARHPQIKTIPILDERSAAFFALGLAKSSGLPTVLVCT
SGTAGANFYPAVIEAYQSAVPLIIFTGDRPPELRDCHSGQTIDQIKLFGNFAQWFSEMATPLGTVEMLRYARQTMVQAWR
RSQFPQKGVVHLNCPFRDPLAPLADGSVTHLKDVLKADFFAGIQPLEKAEFSADILPTEAWKNYARGLIIAGVNQPDDPD
VYCQAIAQLSGYLSFPVLTEALSPVRNYAHYFPQGLVITYDFILRDQNLAETLKPEIVIQIGALPTSKILRAWLEAQQIQ
TYILSERADNFDPLHRPHQYLAVNLRTMKYLGIFPDNEKGLPYLDLWQTQDAKIRAKLAAVFQAEQNLNEAAIAYCLARA
LPEETAIFFANSMVVRYAEFFWQLNDQQILPYFNRGANGIDGTLSTALGIAENHSPAVLLTGDLALLHDINGFLTLSKFT
GSLTIIVINNQGGGIFEMLPIAEEKDIFEDYFATPQTVEFSKVCDLYGIEYQAIQTKADLMQTCAILPPRGVRLLEVKTN
RNQAMTWLKDLFQGFQSHF

Sequences:

>Translated_579_residues
MGLDFSNLNSLWAGVMVETLARLGLQSAVICPGSRSTPLTVALARHPQIKTIPILDERSAAFFALGLAKSSGLPTVLVCT
SGTAGANFYPAVIEAYQSAVPLIIFTGDRPPELRDCHSGQTIDQIKLFGNFAQWFSEMATPLGTVEMLRYARQTMVQAWR
RSQFPQKGVVHLNCPFRDPLAPLADGSVTHLKDVLKADFFAGIQPLEKAEFSADILPTEAWKNYARGLIIAGVNQPDDPD
VYCQAIAQLSGYLSFPVLTEALSPVRNYAHYFPQGLVITYDFILRDQNLAETLKPEIVIQIGALPTSKILRAWLEAQQIQ
TYILSERADNFDPLHRPHQYLAVNLRTMKYLGIFPDNEKGLPYLDLWQTQDAKIRAKLAAVFQAEQNLNEAAIAYCLARA
LPEETAIFFANSMVVRYAEFFWQLNDQQILPYFNRGANGIDGTLSTALGIAENHSPAVLLTGDLALLHDINGFLTLSKFT
GSLTIIVINNQGGGIFEMLPIAEEKDIFEDYFATPQTVEFSKVCDLYGIEYQAIQTKADLMQTCAILPPRGVRLLEVKTN
RNQAMTWLKDLFQGFQSHF
>Mature_578_residues
GLDFSNLNSLWAGVMVETLARLGLQSAVICPGSRSTPLTVALARHPQIKTIPILDERSAAFFALGLAKSSGLPTVLVCTS
GTAGANFYPAVIEAYQSAVPLIIFTGDRPPELRDCHSGQTIDQIKLFGNFAQWFSEMATPLGTVEMLRYARQTMVQAWRR
SQFPQKGVVHLNCPFRDPLAPLADGSVTHLKDVLKADFFAGIQPLEKAEFSADILPTEAWKNYARGLIIAGVNQPDDPDV
YCQAIAQLSGYLSFPVLTEALSPVRNYAHYFPQGLVITYDFILRDQNLAETLKPEIVIQIGALPTSKILRAWLEAQQIQT
YILSERADNFDPLHRPHQYLAVNLRTMKYLGIFPDNEKGLPYLDLWQTQDAKIRAKLAAVFQAEQNLNEAAIAYCLARAL
PEETAIFFANSMVVRYAEFFWQLNDQQILPYFNRGANGIDGTLSTALGIAENHSPAVLLTGDLALLHDINGFLTLSKFTG
SLTIIVINNQGGGIFEMLPIAEEKDIFEDYFATPQTVEFSKVCDLYGIEYQAIQTKADLMQTCAILPPRGVRLLEVKTNR
NQAMTWLKDLFQGFQSHF

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=546, Percent_Identity=31.1355311355311, Blast_Score=224, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 64365; Mature: 64233

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLDFSNLNSLWAGVMVETLARLGLQSAVICPGSRSTPLTVALARHPQIKTIPILDERSA
CCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEECCCCC
AFFALGLAKSSGLPTVLVCTSGTAGANFYPAVIEAYQSAVPLIIFTGDRPPELRDCHSGQ
EEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHCCCCC
TIDQIKLFGNFAQWFSEMATPLGTVEMLRYARQTMVQAWRRSQFPQKGVVHLNCPFRDPL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
APLADGSVTHLKDVLKADFFAGIQPLEKAEFSADILPTEAWKNYARGLIIAGVNQPDDPD
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHCEEEEEECCCCCCHH
VYCQAIAQLSGYLSFPVLTEALSPVRNYAHYFPQGLVITYDFILRDQNLAETLKPEIVIQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHCCCEEEEE
IGALPTSKILRAWLEAQQIQTYILSERADNFDPLHRPHQYLAVNLRTMKYLGIFPDNEKG
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCC
LPYLDLWQTQDAKIRAKLAAVFQAEQNLNEAAIAYCLARALPEETAIFFANSMVVRYAEF
CCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEEEHHHHHHHHHHH
FWQLNDQQILPYFNRGANGIDGTLSTALGIAENHSPAVLLTGDLALLHDINGFLTLSKFT
HHHCCCCEECCHHCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCEECCCCCEEEEEECC
GSLTIIVINNQGGGIFEMLPIAEEKDIFEDYFATPQTVEFSKVCDLYGIEYQAIQTKADL
CEEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
MQTCAILPPRGVRLLEVKTNRNQAMTWLKDLFQGFQSHF
HHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GLDFSNLNSLWAGVMVETLARLGLQSAVICPGSRSTPLTVALARHPQIKTIPILDERSA
CCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEECCCCC
AFFALGLAKSSGLPTVLVCTSGTAGANFYPAVIEAYQSAVPLIIFTGDRPPELRDCHSGQ
EEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHCCCCC
TIDQIKLFGNFAQWFSEMATPLGTVEMLRYARQTMVQAWRRSQFPQKGVVHLNCPFRDPL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
APLADGSVTHLKDVLKADFFAGIQPLEKAEFSADILPTEAWKNYARGLIIAGVNQPDDPD
CCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHCEEEEEECCCCCCHH
VYCQAIAQLSGYLSFPVLTEALSPVRNYAHYFPQGLVITYDFILRDQNLAETLKPEIVIQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHCCCEEEEE
IGALPTSKILRAWLEAQQIQTYILSERADNFDPLHRPHQYLAVNLRTMKYLGIFPDNEKG
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEECCCCCCC
LPYLDLWQTQDAKIRAKLAAVFQAEQNLNEAAIAYCLARALPEETAIFFANSMVVRYAEF
CCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEEEHHHHHHHHHHH
FWQLNDQQILPYFNRGANGIDGTLSTALGIAENHSPAVLLTGDLALLHDINGFLTLSKFT
HHHCCCCEECCHHCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCEECCCCCEEEEEECC
GSLTIIVINNQGGGIFEMLPIAEEKDIFEDYFATPQTVEFSKVCDLYGIEYQAIQTKADL
CEEEEEEEECCCCCEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHH
MQTCAILPPRGVRLLEVKTNRNQAMTWLKDLFQGFQSHF
HHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA