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The map label for this gene is pcnB [H]

Identifier: 170078104

GI number: 170078104

Start: 1576982

End: 1578205

Strand: Reverse

Name: pcnB [H]

Synonym: SYNPCC7002_A1495

Alternate gene names: 170078104

Gene position: 1578205-1576982 (Counterclockwise)

Preceding gene: 170078107

Following gene: 170078102

Centisome position: 52.47

GC content: 53.76

Gene sequence:

>1224_bases
TTGGACTCTCCCAAGGAGCGTTTAGCTGAATTTTTACGTCTTTTGCCATTTTCTGTGGCGGAATTGCCAGCGGAGGCTTG
CTTGGTGGGTGGTGCCGTGCGGGATGCCCTCTTGCAGCGCCAGAGTGATTATATTGATTTTGATTTCGTCGTCCCCAAAA
AGGCAGTGGAAACGGCCCGTGCCATTGCAAAAAAATATCAAGCGGGTTTTGTGGTACTCGATGAGATGCGCCAAATTGCC
CGGGTGGTCTTCCGGCAGGGGACGTTGGATTTTGCCCAACAGGAAGGAGACAGCCTAGAGCGGGATTTGCGGCGGCGGGA
TTATTGTGTGAATGCGATCGCCTACCATATCCGTTCTGATTGCTTAGTCGATCCCCTCAATGGCCTCGCTGATTTGGAAA
ATAAGGTGTTGCGGATGGTGACACCGGAAAATTTGGCCGACGACCCCCTGCGGTTGTTGCGGGCCTATCGCCAGGCGGCG
CAGTTGGGCTTTACCTTAGATGCGGCTACCAGGGCACAAATTCGCGAGTTGGCCCCTTTGATTAAAAATATTGCAGCCGA
AAGAATTCAGCATGAGTTGAACTATTTGTTGCACCACCCACAAGGCGATCGCTGGCTAGAGTTGGCCTTTCAGGATGGCT
TGTTGGCCCATTGGTTGCCCGCAGCGGATCAGACGAGCTTAGACCGCATGCAACGGTTAACGGCCATTGGTCAAGAATTC
GCCGCAGTCTACCCCGAATTTGCCACGGAGACAGCTTGGCTCGCCCTAGGGAAATTGGCCTGTTTGGTGGGCAGCGATCC
AGAAAAAGCCCAACAGGCGATCGCCCGCTTGAAATATCCCCGGTACGAACTGCGCACCGTCAGCACCGTCATCGAGATGC
TCCCCCAACTGTTGGCCAGCCAAGCGCAACCCCTGAGCCTGCGAGAACAATATTTTTTCTTCCAAACGGTTAAGGGCACT
TTTCCGATCCTTGCCCTGGTTGCCCGGAGTTTTGGCCTCAGCAGTGCCTCGTTAGCACCATTACTGCAACGGTATTTCAC
CGCCCATGATCCCGTCGCCCATCCGGTTTCGCTTTTATCAGGGAAGGATCTCATCCATGAGTTGCACCTTTCCCCCAGTC
CCCAAATCGGTCATTTACTCACCGAAATTCAAGTGGCCCATGTCGAAGGAAAAATTCACACCAAAGCCGAAGCGATCGAA
TGGGGGCGATCGCAACTGCACTAG

Upstream 100 bases:

>100_bases
CTGTGTTTTTGCGCTTTTGAATTTCCCTGCCCATAATCGGAGGGGGAATGATATTCTCATTAATCGTGCTTATTCTAATC
GGGTTTCATCATTGATGGTT

Downstream 100 bases:

>100_bases
CCACAAAATACAAAAAAATGCAGCCAAGCATCATCCTGGACTGCACTTTATAGATTATGCAATTGAGACTGCAATTAGAA
ATTAGGGGGTAATGTAACCC

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MDSPKERLAEFLRLLPFSVAELPAEACLVGGAVRDALLQRQSDYIDFDFVVPKKAVETARAIAKKYQAGFVVLDEMRQIA
RVVFRQGTLDFAQQEGDSLERDLRRRDYCVNAIAYHIRSDCLVDPLNGLADLENKVLRMVTPENLADDPLRLLRAYRQAA
QLGFTLDAATRAQIRELAPLIKNIAAERIQHELNYLLHHPQGDRWLELAFQDGLLAHWLPAADQTSLDRMQRLTAIGQEF
AAVYPEFATETAWLALGKLACLVGSDPEKAQQAIARLKYPRYELRTVSTVIEMLPQLLASQAQPLSLREQYFFFQTVKGT
FPILALVARSFGLSSASLAPLLQRYFTAHDPVAHPVSLLSGKDLIHELHLSPSPQIGHLLTEIQVAHVEGKIHTKAEAIE
WGRSQLH

Sequences:

>Translated_407_residues
MDSPKERLAEFLRLLPFSVAELPAEACLVGGAVRDALLQRQSDYIDFDFVVPKKAVETARAIAKKYQAGFVVLDEMRQIA
RVVFRQGTLDFAQQEGDSLERDLRRRDYCVNAIAYHIRSDCLVDPLNGLADLENKVLRMVTPENLADDPLRLLRAYRQAA
QLGFTLDAATRAQIRELAPLIKNIAAERIQHELNYLLHHPQGDRWLELAFQDGLLAHWLPAADQTSLDRMQRLTAIGQEF
AAVYPEFATETAWLALGKLACLVGSDPEKAQQAIARLKYPRYELRTVSTVIEMLPQLLASQAQPLSLREQYFFFQTVKGT
FPILALVARSFGLSSASLAPLLQRYFTAHDPVAHPVSLLSGKDLIHELHLSPSPQIGHLLTEIQVAHVEGKIHTKAEAIE
WGRSQLH
>Mature_407_residues
MDSPKERLAEFLRLLPFSVAELPAEACLVGGAVRDALLQRQSDYIDFDFVVPKKAVETARAIAKKYQAGFVVLDEMRQIA
RVVFRQGTLDFAQQEGDSLERDLRRRDYCVNAIAYHIRSDCLVDPLNGLADLENKVLRMVTPENLADDPLRLLRAYRQAA
QLGFTLDAATRAQIRELAPLIKNIAAERIQHELNYLLHHPQGDRWLELAFQDGLLAHWLPAADQTSLDRMQRLTAIGQEF
AAVYPEFATETAWLALGKLACLVGSDPEKAQQAIARLKYPRYELRTVSTVIEMLPQLLASQAQPLSLREQYFFFQTVKGT
FPILALVARSFGLSSASLAPLLQRYFTAHDPVAHPVSLLSGKDLIHELHLSPSPQIGHLLTEIQVAHVEGKIHTKAEAIE
WGRSQLH

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081691, Length=203, Percent_Identity=30.5418719211823, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789436, Length=182, Percent_Identity=35.1648351648352, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45709; Mature: 45709

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSPKERLAEFLRLLPFSVAELPAEACLVGGAVRDALLQRQSDYIDFDFVVPKKAVETAR
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
AIAKKYQAGFVVLDEMRQIARVVFRQGTLDFAQQEGDSLERDLRRRDYCVNAIAYHIRSD
HHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
CLVDPLNGLADLENKVLRMVTPENLADDPLRLLRAYRQAAQLGFTLDAATRAQIRELAPL
CHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IKNIAAERIQHELNYLLHHPQGDRWLELAFQDGLLAHWLPAADQTSLDRMQRLTAIGQEF
HHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHH
AAVYPEFATETAWLALGKLACLVGSDPEKAQQAIARLKYPRYELRTVSTVIEMLPQLLAS
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
QAQPLSLREQYFFFQTVKGTFPILALVARSFGLSSASLAPLLQRYFTAHDPVAHPVSLLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
GKDLIHELHLSPSPQIGHLLTEIQVAHVEGKIHTKAEAIEWGRSQLH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDSPKERLAEFLRLLPFSVAELPAEACLVGGAVRDALLQRQSDYIDFDFVVPKKAVETAR
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHH
AIAKKYQAGFVVLDEMRQIARVVFRQGTLDFAQQEGDSLERDLRRRDYCVNAIAYHIRSD
HHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHC
CLVDPLNGLADLENKVLRMVTPENLADDPLRLLRAYRQAAQLGFTLDAATRAQIRELAPL
CHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
IKNIAAERIQHELNYLLHHPQGDRWLELAFQDGLLAHWLPAADQTSLDRMQRLTAIGQEF
HHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHH
AAVYPEFATETAWLALGKLACLVGSDPEKAQQAIARLKYPRYELRTVSTVIEMLPQLLAS
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
QAQPLSLREQYFFFQTVKGTFPILALVARSFGLSSASLAPLLQRYFTAHDPVAHPVSLLS
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
GKDLIHELHLSPSPQIGHLLTEIQVAHVEGKIHTKAEAIEWGRSQLH
HHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11442348 [H]