| Definition | Synechococcus sp. PCC 7002 chromosome, complete genome. |
|---|---|
| Accession | NC_010475 |
| Length | 3,008,047 |
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The map label for this gene is anfA [H]
Identifier: 170078084
GI number: 170078084
Start: 1552030
End: 1554552
Strand: Direct
Name: anfA [H]
Synonym: SYNPCC7002_A1475
Alternate gene names: 170078084
Gene position: 1552030-1554552 (Clockwise)
Preceding gene: 170078074
Following gene: 170078085
Centisome position: 51.6
GC content: 52.95
Gene sequence:
>2523_bases ATGACCATCCAGGAACGTTTGATTTGGTTGCAGGAGCGCACCAGCCTCGGTTGTCTGTCGCTGGAAACCCTAGGGGCGAT CGCCACGGCACTCCAAAGGCAAACCCTCCCTCCCCAACAGACCCTCAGCGAGATCGACCAACATCCCCAAGGTTTATACA TTTTGATTTCCGGGGAGGTGATCAGCCACAACAGCGCGGCTGCACCAGAGACCAACCCCCTAACCAGAGGCAGTACGATC AATCTGCGGCCTTTACTCCTTAATGAAGCTTCCCAAGAGGCGATCGCCACCGTCACCGCCACAGAAATGTGGTTTATGCC GCGCCAACAGTGGCAAGACCTGATCCAAAACTATCCTGAAATTACCCAGGCCTTTTCCCAGGATCTCGCCCAGGAGCTGG CAGAAGTCGCCCAAGCCCTCCAACAGGAACAGCAACAACAGGAAATTTTGCGCCCCTACCTCGTCACCAAAGCGAAACGG GGGGTTGTGGGGAAAAGTCGCTACGCTAAACGCCTGCGGCAACAAATTCGTGAGGCGGGTACTCATGATCGATCCATTTA CATTTTTGGGGAACCGGGTTTAGAAAAAGATAATTTAGCCGCCCTGATCCACTACGGTTCGCCCCGACGCCGACAGCCGA TTATTAAAATCGATTGCAGTATTCTCCAAAAAAGTGGTGCTGAACTCTTTGGCCGCACCGATGGCAAAAAAGGTCTCTTG GCATGGCTGGGAAAAGGCACCCTAATTCTCAACAAAATTGAAGAATTGCCCCCGGAACTGATCGATAAAGTCGCCACGTT AATCGAAACCCAGACCTATACCCCCATCAGTCGCCCCGGTGATCCAGAACCTGTCCCCCAACAAACGGCGGCAAAATTGG TGGCGATCGCCGAACGACAACTTCCTGCTGTGGCCCGCCATTTTTCCGAAACGATCAAGGTGCCACCGCTGCGGGTTCGC AAAGCAGATCTAGCGGCCCAGGTGGAATATTACACCAGCTTATTTTGCCGGGCGAAGGGCCTCGATAAACCGACCCTCAC CCCCGAAGCCCTCCGTCGTCTCCAGTCCTACGACTTTCCGAACAATATCCGCGAGTTGCAAAACTTGGTAGAACGGGCCA TTGTCCAATCGGATCAGGGGTCGATGCTCACCGAAGAAATTTTTTGGTCAGCCCAGGGGAAACAAAAACGATTTCGCCTC AATTTACTCAATGCCTACCCTGCCTTCCGGCGTTTTCTCCGGAGCCCTTGGTGGCCCGACCGCATTAACTATGGTTTTAC GGCGATCGCCTTTCCATTAATTATTGCTGTTCTATTTCTCGCCCCCCAAACCCGCGACCAAAACTTTGCGTTGAATTTCT TTTGGGCTTGGTGGTGGCCCCTGGTGCTTCTCGCTTTTCCCTTTGTCGGTCGTCTTTGGTGTGCCGTTTGTCCGTTTATG ATCTACGGGGAAATTACCCAGAAAATCACCACCGAACTCCTAAAGCGCGACCTCAAACCCTGGCCGCGTCAGCAGGCAGA AAAATGGGGCGGTTGGTTTCTCTTTGGCTTATTTGCCCTGATTTTCCTCTGGGAAGAACTGTGGGATTTACAAAATACGG CTTATCTTTCGGCTTGGTTGCTGATTATTATTACTGCCGGGGCGATGATCTGCTCGGCGATCTTTGAGCGGCGGTTTTGG TGTCGCTATCTTTGTCCCATCGGCGGCATGAATGGCCTCTTTGCGAAACTCTCGATGACGGAGTTGCGGGCGCAACAGGG CACTTGTTCGGCGGAATGTAGCACCTATCAATGCTATAAAGGCGGCCCCCAAAAGGGTGAAGGCTTAGAAACAGCAGGCT GTCCCCTCTATTCCCACCCGGCCCAACTGGAGGACAACCGCGATTGTGTACTGTGCATGACCTGTCTTAAGGCTTGTCCC CATCGATCAGTAGCGGTAAATCTACGCCCCCCAGCCATTGAACTGTGGACAACCCACCGCCCCCGCACCTATGAAGTCGC TCTTTTACTATTACTCCTGGGGGGAATCTTTCTCCATCGGCTGCCAGAAATTAACCAAATCCTGGGTTTAAATTTAGACC TCAGTCAATTTTGGCCCCACCTGGGATTGGCGATCGCCGCCTTGGGAGTACCCGCTGGAGTAGTCCTATTAACAGGGGCT GTGGTGCAATTTTTAAACTCTCCCCCCAAGGCAAAACCCTTTTTACAACTGGCCTATGGTTATCTACCCTTGGTTTTGGG GGGGAGCCTCGCCCATTATCTCCGTCTCGGTTTAGGGGAAGCCGGTCAAATTTTACCCGTAACCATGGCCACCTTCGGTC TCCAGGGCAGCACCTTGCCGATTGTTGTCGCCCATCCCGCCGTGATTGCCTTTCTCCAGGGTTCAACCCTCGTCTTCGGG ACGCTCTGTACTTTTGTGCTCACGAAAAAAATGAGTAAACAATCCTGGCGATCGCTGTTGCCCCAGTACCTAACAAGTCT CGGTCTCGCCTGGGCGATGGCTCAGATTATTGTCGGTTTGTAA
Upstream 100 bases:
>100_bases TGGCGGGTCATTGGTTCCACTGTAGCTTGTTCCGCTGGGGAATTAATCTACTCCGTCAGATTCGATAAGCTGGGGGAGCA AGGGTTGGAGGAAAATAAAC
Downstream 100 bases:
>100_bases CAGATTTTTGTTACAAAACCCCAGGGCTTAACACCATTTTTTCAACCGTTATTGCTCTACCCCGATCCCCTGAAAGGATT GGGGTTACTACTGTTTGGGG
Product: nitrogen assimilation regulatory protein
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 840; Mature: 839
Protein sequence:
>840_residues MTIQERLIWLQERTSLGCLSLETLGAIATALQRQTLPPQQTLSEIDQHPQGLYILISGEVISHNSAAAPETNPLTRGSTI NLRPLLLNEASQEAIATVTATEMWFMPRQQWQDLIQNYPEITQAFSQDLAQELAEVAQALQQEQQQQEILRPYLVTKAKR GVVGKSRYAKRLRQQIREAGTHDRSIYIFGEPGLEKDNLAALIHYGSPRRRQPIIKIDCSILQKSGAELFGRTDGKKGLL AWLGKGTLILNKIEELPPELIDKVATLIETQTYTPISRPGDPEPVPQQTAAKLVAIAERQLPAVARHFSETIKVPPLRVR KADLAAQVEYYTSLFCRAKGLDKPTLTPEALRRLQSYDFPNNIRELQNLVERAIVQSDQGSMLTEEIFWSAQGKQKRFRL NLLNAYPAFRRFLRSPWWPDRINYGFTAIAFPLIIAVLFLAPQTRDQNFALNFFWAWWWPLVLLAFPFVGRLWCAVCPFM IYGEITQKITTELLKRDLKPWPRQQAEKWGGWFLFGLFALIFLWEELWDLQNTAYLSAWLLIIITAGAMICSAIFERRFW CRYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQCYKGGPQKGEGLETAGCPLYSHPAQLEDNRDCVLCMTCLKACP HRSVAVNLRPPAIELWTTHRPRTYEVALLLLLLGGIFLHRLPEINQILGLNLDLSQFWPHLGLAIAALGVPAGVVLLTGA VVQFLNSPPKAKPFLQLAYGYLPLVLGGSLAHYLRLGLGEAGQILPVTMATFGLQGSTLPIVVAHPAVIAFLQGSTLVFG TLCTFVLTKKMSKQSWRSLLPQYLTSLGLAWAMAQIIVGL
Sequences:
>Translated_840_residues MTIQERLIWLQERTSLGCLSLETLGAIATALQRQTLPPQQTLSEIDQHPQGLYILISGEVISHNSAAAPETNPLTRGSTI NLRPLLLNEASQEAIATVTATEMWFMPRQQWQDLIQNYPEITQAFSQDLAQELAEVAQALQQEQQQQEILRPYLVTKAKR GVVGKSRYAKRLRQQIREAGTHDRSIYIFGEPGLEKDNLAALIHYGSPRRRQPIIKIDCSILQKSGAELFGRTDGKKGLL AWLGKGTLILNKIEELPPELIDKVATLIETQTYTPISRPGDPEPVPQQTAAKLVAIAERQLPAVARHFSETIKVPPLRVR KADLAAQVEYYTSLFCRAKGLDKPTLTPEALRRLQSYDFPNNIRELQNLVERAIVQSDQGSMLTEEIFWSAQGKQKRFRL NLLNAYPAFRRFLRSPWWPDRINYGFTAIAFPLIIAVLFLAPQTRDQNFALNFFWAWWWPLVLLAFPFVGRLWCAVCPFM IYGEITQKITTELLKRDLKPWPRQQAEKWGGWFLFGLFALIFLWEELWDLQNTAYLSAWLLIIITAGAMICSAIFERRFW CRYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQCYKGGPQKGEGLETAGCPLYSHPAQLEDNRDCVLCMTCLKACP HRSVAVNLRPPAIELWTTHRPRTYEVALLLLLLGGIFLHRLPEINQILGLNLDLSQFWPHLGLAIAALGVPAGVVLLTGA VVQFLNSPPKAKPFLQLAYGYLPLVLGGSLAHYLRLGLGEAGQILPVTMATFGLQGSTLPIVVAHPAVIAFLQGSTLVFG TLCTFVLTKKMSKQSWRSLLPQYLTSLGLAWAMAQIIVGL >Mature_839_residues TIQERLIWLQERTSLGCLSLETLGAIATALQRQTLPPQQTLSEIDQHPQGLYILISGEVISHNSAAAPETNPLTRGSTIN LRPLLLNEASQEAIATVTATEMWFMPRQQWQDLIQNYPEITQAFSQDLAQELAEVAQALQQEQQQQEILRPYLVTKAKRG VVGKSRYAKRLRQQIREAGTHDRSIYIFGEPGLEKDNLAALIHYGSPRRRQPIIKIDCSILQKSGAELFGRTDGKKGLLA WLGKGTLILNKIEELPPELIDKVATLIETQTYTPISRPGDPEPVPQQTAAKLVAIAERQLPAVARHFSETIKVPPLRVRK ADLAAQVEYYTSLFCRAKGLDKPTLTPEALRRLQSYDFPNNIRELQNLVERAIVQSDQGSMLTEEIFWSAQGKQKRFRLN LLNAYPAFRRFLRSPWWPDRINYGFTAIAFPLIIAVLFLAPQTRDQNFALNFFWAWWWPLVLLAFPFVGRLWCAVCPFMI YGEITQKITTELLKRDLKPWPRQQAEKWGGWFLFGLFALIFLWEELWDLQNTAYLSAWLLIIITAGAMICSAIFERRFWC RYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQCYKGGPQKGEGLETAGCPLYSHPAQLEDNRDCVLCMTCLKACPH RSVAVNLRPPAIELWTTHRPRTYEVALLLLLLGGIFLHRLPEINQILGLNLDLSQFWPHLGLAIAALGVPAGVVLLTGAV VQFLNSPPKAKPFLQLAYGYLPLVLGGSLAHYLRLGLGEAGQILPVTMATFGLQGSTLPIVVAHPAVIAFLQGSTLVFGT LCTFVLTKKMSKQSWRSLLPQYLTSLGLAWAMAQIIVGL
Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]
COG id: COG0348
COG function: function code C; Polyferredoxin
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 sigma-54 factor interaction domain [H]
Homologues:
Organism=Escherichia coli, GI87082117, Length=274, Percent_Identity=29.1970802919708, Blast_Score=106, Evalue=6e-24, Organism=Escherichia coli, GI1788550, Length=224, Percent_Identity=29.0178571428571, Blast_Score=94, Evalue=5e-20, Organism=Escherichia coli, GI1789233, Length=230, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=1e-19, Organism=Escherichia coli, GI87081872, Length=242, Percent_Identity=28.099173553719, Blast_Score=92, Evalue=2e-19, Organism=Escherichia coli, GI1790437, Length=242, Percent_Identity=28.9256198347107, Blast_Score=90, Evalue=7e-19, Organism=Escherichia coli, GI87082152, Length=253, Percent_Identity=30.0395256916996, Blast_Score=89, Evalue=1e-18, Organism=Escherichia coli, GI1789087, Length=235, Percent_Identity=26.3829787234043, Blast_Score=86, Evalue=1e-17, Organism=Escherichia coli, GI1787583, Length=241, Percent_Identity=24.896265560166, Blast_Score=79, Evalue=2e-15, Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=27.9166666666667, Blast_Score=78, Evalue=2e-15, Organism=Escherichia coli, GI1790299, Length=266, Percent_Identity=25.5639097744361, Blast_Score=68, Evalue=3e-12, Organism=Escherichia coli, GI87081858, Length=244, Percent_Identity=30.327868852459, Blast_Score=64, Evalue=4e-11,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003593 - InterPro: IPR003018 - InterPro: IPR020441 - InterPro: IPR009057 - InterPro: IPR002197 - InterPro: IPR002078 [H]
Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]
EC number: NA
Molecular weight: Translated: 94292; Mature: 94161
Theoretical pI: Translated: 8.94; Mature: 8.94
Prosite motif: PS50042 CNMP_BINDING_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00198 4FE4S_FERREDOXIN
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.0 %Cys (Translated Protein) 1.4 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 2.0 %Cys (Mature Protein) 1.3 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTIQERLIWLQERTSLGCLSLETLGAIATALQRQTLPPQQTLSEIDQHPQGLYILISGEV CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCE ISHNSAAAPETNPLTRGSTINLRPLLLNEASQEAIATVTATEMWFMPRQQWQDLIQNYPE EECCCCCCCCCCCCCCCCCEEECHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCHH ITQAFSQDLAQELAEVAQALQQEQQQQEILRPYLVTKAKRGVVGKSRYAKRLRQQIREAG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC THDRSIYIFGEPGLEKDNLAALIHYGSPRRRQPIIKIDCSILQKSGAELFGRTDGKKGLL CCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEHHHHHHHCCHHHHCCCCCCCCHH AWLGKGTLILNKIEELPPELIDKVATLIETQTYTPISRPGDPEPVPQQTAAKLVAIAERQ HHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH LPAVARHFSETIKVPPLRVRKADLAAQVEYYTSLFCRAKGLDKPTLTPEALRRLQSYDFP HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCC NNIRELQNLVERAIVQSDQGSMLTEEIFWSAQGKQKRFRLNLLNAYPAFRRFLRSPWWPD HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC RINYGFTAIAFPLIIAVLFLAPQTRDQNFALNFFWAWWWPLVLLAFPFVGRLWCAVCPFM CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH IYGEITQKITTELLKRDLKPWPRQQAEKWGGWFLFGLFALIFLWEELWDLQNTAYLSAWL HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH LIIITAGAMICSAIFERRFWCRYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQCYK HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCC GGPQKGEGLETAGCPLYSHPAQLEDNRDCVLCMTCLKACPHRSVAVNLRPPAIELWTTHR CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCEEEEECCCC PRTYEVALLLLLLGGIFLHRLPEINQILGLNLDLSQFWPHLGLAIAALGVPAGVVLLTGA CCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH VVQFLNSPPKAKPFLQLAYGYLPLVLGGSLAHYLRLGLGEAGQILPVTMATFGLQGSTLP HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCCCCCE IVVAHPAVIAFLQGSTLVFGTLCTFVLTKKMSKQSWRSLLPQYLTSLGLAWAMAQIIVGL EEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure TIQERLIWLQERTSLGCLSLETLGAIATALQRQTLPPQQTLSEIDQHPQGLYILISGEV CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCE ISHNSAAAPETNPLTRGSTINLRPLLLNEASQEAIATVTATEMWFMPRQQWQDLIQNYPE EECCCCCCCCCCCCCCCCCEEECHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCHH ITQAFSQDLAQELAEVAQALQQEQQQQEILRPYLVTKAKRGVVGKSRYAKRLRQQIREAG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC THDRSIYIFGEPGLEKDNLAALIHYGSPRRRQPIIKIDCSILQKSGAELFGRTDGKKGLL CCCCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEHHHHHHHCCHHHHCCCCCCCCHH AWLGKGTLILNKIEELPPELIDKVATLIETQTYTPISRPGDPEPVPQQTAAKLVAIAERQ HHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH LPAVARHFSETIKVPPLRVRKADLAAQVEYYTSLFCRAKGLDKPTLTPEALRRLQSYDFP HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCC NNIRELQNLVERAIVQSDQGSMLTEEIFWSAQGKQKRFRLNLLNAYPAFRRFLRSPWWPD HHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC RINYGFTAIAFPLIIAVLFLAPQTRDQNFALNFFWAWWWPLVLLAFPFVGRLWCAVCPFM CCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH IYGEITQKITTELLKRDLKPWPRQQAEKWGGWFLFGLFALIFLWEELWDLQNTAYLSAWL HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH LIIITAGAMICSAIFERRFWCRYLCPIGGMNGLFAKLSMTELRAQQGTCSAECSTYQCYK HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEECCC GGPQKGEGLETAGCPLYSHPAQLEDNRDCVLCMTCLKACPHRSVAVNLRPPAIELWTTHR CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCEEEEECCCC PRTYEVALLLLLLGGIFLHRLPEINQILGLNLDLSQFWPHLGLAIAALGVPAGVVLLTGA CCHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH VVQFLNSPPKAKPFLQLAYGYLPLVLGGSLAHYLRLGLGEAGQILPVTMATFGLQGSTLP HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHCCCCCCCE IVVAHPAVIAFLQGSTLVFGTLCTFVLTKKMSKQSWRSLLPQYLTSLGLAWAMAQIIVGL EEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 2722750 [H]