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The map label for this gene is pilT [H]

Identifier: 170077828

GI number: 170077828

Start: 1254430

End: 1255776

Strand: Reverse

Name: pilT [H]

Synonym: SYNPCC7002_A1212

Alternate gene names: 170077828

Gene position: 1255776-1254430 (Counterclockwise)

Preceding gene: 170077831

Following gene: 170077827

Centisome position: 41.75

GC content: 51.6

Gene sequence:

>1347_bases
ATGCCTCCGAACCGTTCTGCGGGCTCCCGTCCTCCCGTTCCCCCTGCCAGCGAGGCAACCCAAATTATCTCTGCACGGCC
CCAACCTGCTGTTTCGGCGGATGAAACACAAATTGTGATGCCCCAACAGCGACGTCCCCAGCCCACGGCCCAACCCCAAC
AACGTCGCCCGGCCTCTCCACCTCCGCCACCGCCGCTGCAACGGATGCCGCCCCCACCGCCTTTACCTACGCCACCTCGA
CAATATTTAGGGGCTAATGCCGAACGAATTCCCCAACGGGGGCCAGGGCAACCGAGTTTAAAGGAACTGGTCACCAGGGC
TCACAAAGAAGGATTTTCCGATGTGCACCTTGGGGTCGGGGAAGTGCCTCGGTATCGTGATCGGGGGGAAATGGTGATCA
CTGAATACCCTGTGACTGACCAAGATACGTTTTATGCTTGGCTGCATGAAGTGCTTAAACCACAGGAAATCCAACGCTTT
AAGGACAGTCTCGATTTTGATGGCGCAACCCAATACGAATTCGCCCGGGTGCGGATCAATATTTTTGATTCTCTAAATGG
CCCCTCGTTGGTCTTGCGTTTGATTCCGCTCAAAATCTTGACCATGGAGCAGTTGCGCCTACCACCCATCTTTAAGAATA
TTTCTGATGCAGAAAAAGGCCTCGTTCTGGTTACAGGGCCAACGGGTTCGGGTAAATCCACAACCATGGCGGCAATGGTA
GATTACATCAACAAAACCCACCCAAAGAATATCATCACCATTGAAGATCCGATTGAATTTGTTCACCAAAGTCGGAAATC
TTTGATCAAGCACCGGGAAGTGGGGATGCACACCCTAAAGTTTGACAACGCGCTCAAAGCGGCCCTCCGGGAAGACCCAG
ACATTATCCTGGTTGGGGAAATGCGGGATAAAGAAACGGTTAATACGGCCCTCAAAGCAGCGCAAACGGGGCACCTTGTC
ATGGGAACGCTCCATACCAACAGTGCGATTAAAACTATTGAACGGATTCTTAATCTCTACAGTGCAGATGAGCAAAATGC
GATGAAGGTGGCGATCGCCGAATCCTTGGTTTCTGTCATCGCCCAAGGGCTATGCCGTACCACCGATGGAAAGCGGGCTG
CTTACCATGACATTTTAGTGAATACCGAAACCATGAAAGATTACATCGTCAGCGGTCAGTACGACAAAATGCTTGAACTG
ATGCAAAATGGTGAATTTGATGGTATGGTCACCATGAATCAGTCGTTGTTTAACCTCTACCAAGAAGGCCGGATTACAGA
GGAAATCGCCCTAGAAAAATCGCCGACGCCCAACGAAATGGCGATGATGCTCCGGGGACGGATCTAG

Upstream 100 bases:

>100_bases
ATAAATTTAGGCAAAATTCCCCGGTTAAACCCACACAACACCAGGGTGTCCCCTCATTTTCAGTCCCCTCCATTGCGGAA
AGATACCTATGAGTGATTCC

Downstream 100 bases:

>100_bases
GCAGAGACCCGTTTCTTGGTAAGCTTGTAACGAATTGTAACGATCAAGAAGTTATGTCCGATTCTCATTCTCTATTTTTG
GCAGTTGCTGGCCAACCCTT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MPPNRSAGSRPPVPPASEATQIISARPQPAVSADETQIVMPQQRRPQPTAQPQQRRPASPPPPPPLQRMPPPPPLPTPPR
QYLGANAERIPQRGPGQPSLKELVTRAHKEGFSDVHLGVGEVPRYRDRGEMVITEYPVTDQDTFYAWLHEVLKPQEIQRF
KDSLDFDGATQYEFARVRINIFDSLNGPSLVLRLIPLKILTMEQLRLPPIFKNISDAEKGLVLVTGPTGSGKSTTMAAMV
DYINKTHPKNIITIEDPIEFVHQSRKSLIKHREVGMHTLKFDNALKAALREDPDIILVGEMRDKETVNTALKAAQTGHLV
MGTLHTNSAIKTIERILNLYSADEQNAMKVAIAESLVSVIAQGLCRTTDGKRAAYHDILVNTETMKDYIVSGQYDKMLEL
MQNGEFDGMVTMNQSLFNLYQEGRITEEIALEKSPTPNEMAMMLRGRI

Sequences:

>Translated_448_residues
MPPNRSAGSRPPVPPASEATQIISARPQPAVSADETQIVMPQQRRPQPTAQPQQRRPASPPPPPPLQRMPPPPPLPTPPR
QYLGANAERIPQRGPGQPSLKELVTRAHKEGFSDVHLGVGEVPRYRDRGEMVITEYPVTDQDTFYAWLHEVLKPQEIQRF
KDSLDFDGATQYEFARVRINIFDSLNGPSLVLRLIPLKILTMEQLRLPPIFKNISDAEKGLVLVTGPTGSGKSTTMAAMV
DYINKTHPKNIITIEDPIEFVHQSRKSLIKHREVGMHTLKFDNALKAALREDPDIILVGEMRDKETVNTALKAAQTGHLV
MGTLHTNSAIKTIERILNLYSADEQNAMKVAIAESLVSVIAQGLCRTTDGKRAAYHDILVNTETMKDYIVSGQYDKMLEL
MQNGEFDGMVTMNQSLFNLYQEGRITEEIALEKSPTPNEMAMMLRGRI
>Mature_447_residues
PPNRSAGSRPPVPPASEATQIISARPQPAVSADETQIVMPQQRRPQPTAQPQQRRPASPPPPPPLQRMPPPPPLPTPPRQ
YLGANAERIPQRGPGQPSLKELVTRAHKEGFSDVHLGVGEVPRYRDRGEMVITEYPVTDQDTFYAWLHEVLKPQEIQRFK
DSLDFDGATQYEFARVRINIFDSLNGPSLVLRLIPLKILTMEQLRLPPIFKNISDAEKGLVLVTGPTGSGKSTTMAAMVD
YINKTHPKNIITIEDPIEFVHQSRKSLIKHREVGMHTLKFDNALKAALREDPDIILVGEMRDKETVNTALKAAQTGHLVM
GTLHTNSAIKTIERILNLYSADEQNAMKVAIAESLVSVIAQGLCRTTDGKRAAYHDILVNTETMKDYIVSGQYDKMLELM
QNGEFDGMVTMNQSLFNLYQEGRITEEIALEKSPTPNEMAMMLRGRI

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=328, Percent_Identity=38.719512195122, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1786296, Length=309, Percent_Identity=33.6569579288026, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1789723, Length=211, Percent_Identity=36.9668246445498, Blast_Score=121, Evalue=7e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 50024; Mature: 49893

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPNRSAGSRPPVPPASEATQIISARPQPAVSADETQIVMPQQRRPQPTAQPQQRRPASP
CCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCC
PPPPPLQRMPPPPPLPTPPRQYLGANAERIPQRGPGQPSLKELVTRAHKEGFSDVHLGVG
CCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCCC
EVPRYRDRGEMVITEYPVTDQDTFYAWLHEVLKPQEIQRFKDSLDFDGATQYEFARVRIN
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEEEEEE
IFDSLNGPSLVLRLIPLKILTMEQLRLPPIFKNISDAEKGLVLVTGPTGSGKSTTMAAMV
EECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCEEEEECCCCCCCHHHHHHHH
DYINKTHPKNIITIEDPIEFVHQSRKSLIKHREVGMHTLKFDNALKAALREDPDIILVGE
HHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCEEEEEC
MRDKETVNTALKAAQTGHLVMGTLHTNSAIKTIERILNLYSADEQNAMKVAIAESLVSVI
CCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AQGLCRTTDGKRAAYHDILVNTETMKDYIVSGQYDKMLELMQNGEFDGMVTMNQSLFNLY
HHHHHCCCCCCHHHHHHHEECHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECHHHHHHH
QEGRITEEIALEKSPTPNEMAMMLRGRI
HHCCCCHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
PPNRSAGSRPPVPPASEATQIISARPQPAVSADETQIVMPQQRRPQPTAQPQQRRPASP
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCHHHCCCCCC
PPPPPLQRMPPPPPLPTPPRQYLGANAERIPQRGPGQPSLKELVTRAHKEGFSDVHLGVG
CCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCCC
EVPRYRDRGEMVITEYPVTDQDTFYAWLHEVLKPQEIQRFKDSLDFDGATQYEFARVRIN
CCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEEEEEEE
IFDSLNGPSLVLRLIPLKILTMEQLRLPPIFKNISDAEKGLVLVTGPTGSGKSTTMAAMV
EECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCEEEEECCCCCCCHHHHHHHH
DYINKTHPKNIITIEDPIEFVHQSRKSLIKHREVGMHTLKFDNALKAALREDPDIILVGE
HHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCCEEEEEC
MRDKETVNTALKAAQTGHLVMGTLHTNSAIKTIERILNLYSADEQNAMKVAIAESLVSVI
CCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
AQGLCRTTDGKRAAYHDILVNTETMKDYIVSGQYDKMLELMQNGEFDGMVTMNQSLFNLY
HHHHHCCCCCCHHHHHHHEECHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECHHHHHHH
QEGRITEEIALEKSPTPNEMAMMLRGRI
HHCCCCHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]