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The map label for this gene is trmE

Identifier: 170077787

GI number: 170077787

Start: 1200936

End: 1202318

Strand: Direct

Name: trmE

Synonym: SYNPCC7002_A1170

Alternate gene names: 170077787

Gene position: 1200936-1202318 (Clockwise)

Preceding gene: 170077786

Following gene: 170077794

Centisome position: 39.92

GC content: 55.39

Gene sequence:

>1383_bases
GTGAACCTAGGCGATACCATTGTTGCGATCGCCAGTGCCGTCGTCCCAAACCAAGGCAGCATTGGCATTGTCCGTCTATC
TGGCGCAGCGGCTCTCCCCATTGCCCGTCAACTCTTTCAAGACCCTGGCCAGCAGACCTGGGAGAGTCACCGCATTCTGT
ACGGCTATGTCCGTCATCCCCAAACCCAAACGATTGTCGATGAAGCGCTGCTCCTGCTGATGTTGGCCCCCCGTTCCTTT
ACCGCTGAAGACGTGGTGGAGTTCCACTGCCACGGGGGCATTATTCCCGTGCAACAGGTGCTACAGCTTTGTTTAGGGGC
AGGGGCGCGGTTGGCGACGCCAGGAGAATTTACCCTGCGCGCCTTTTTGAATGGTCGCATCGATCTCACCCAGGCCGAAA
GTGTCGCGGAATTGGTGGGGGCAAAATCCCCCCAATCGGCCCAGGTGGCCCTAGCAGGCATCCAGGGGAAACTGGCCCAA
CCGATCCAACAACTCCGGGGTCAATGTTTAGATATTTTGGCGGAAGTGGAAGCCCGCATTGATTTTGAAGACGATTTACC
TCCCCTCGATGAGCCGCAAATTCAGCGGGATCTGACCCAGGTGCTCGAAAAAGTGGCACAAATTCTCCAGACCTCTGACC
GGGGCGAACTATTGCGCACGGGGTTAAAGGTAGCGATTGTGGGCCGTCCCAATGTGGGAAAATCGAGTCTGTTAAATGCC
TGGAGTCGCAGCGATCGCGCCATCGTCACGGATTTGCCAGGGACAACGCGGGATGTGGTGGAGTCCCAGCTTGTGGTGCA
GGGTATCCCGGTACAGGTATTGGATACGGCTGGCATCCGGGATACCGAAGATGCCGTGGAAAAAATTGGTGTTGCCCGTT
CCCTTGCCGCTTCTCAGCAGGCGGATTTGATTTTGTTTACCATCGATGCGGCGGTGGGATGGACAGCGGCAGATCAAGAA
ATTTTTCAGCGAATTGTAGCCACAAAAGCCAATCAACCCTTAATTCTGATCCTTAATAAAATTGACATCGGCCAACCGGA
GGCCATTGAAATTCCGCCCCAAGTGCAAGGGGTCGTCAAAACAGCGGCAGCCCAGCACCAGGGTGTGGATGAATTGGAAA
CGGCGATCGCCAATTTGGTGCAAGCGGGGAAAGTGGGGGCTGCGGATCTCGATTTTGCGATCAACCAACGGCAAGCCGCT
GCCTTGGCCCAGGCGCAACAGGCCCTCATCCAAGTACGAGAAACTATTGCCCAAGGGTTGCCCCTGGACTTTTGGACAAT
CGATCTACGCAGTGCGATCCAGGCCCTAGGGGAAATTACCGGCGAAGGGGTGACTGAATCGGTTTTGGATCGGATCTTTA
GCCGTTTTTGTATCGGGAAATAG

Upstream 100 bases:

>100_bases
CAGTAACGTGGTGCCCATCAACCCCATGGCTCCTGCTCCTAGTCCTGCCCCGGCTCCCAGTGAACCGGAACCGAACCTCG
ATGACATCCCGTTTTAGACT

Downstream 100 bases:

>100_bases
GGTCAGGTGCGGATTTTAAACTGGTTAAGATAACTGGCCACTTGGTTCGGTAAAAAAGCCCGTTGGTCAATGAAGTGAAT
CTCTGACTTAAAAGACTCAG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MNLGDTIVAIASAVVPNQGSIGIVRLSGAAALPIARQLFQDPGQQTWESHRILYGYVRHPQTQTIVDEALLLLMLAPRSF
TAEDVVEFHCHGGIIPVQQVLQLCLGAGARLATPGEFTLRAFLNGRIDLTQAESVAELVGAKSPQSAQVALAGIQGKLAQ
PIQQLRGQCLDILAEVEARIDFEDDLPPLDEPQIQRDLTQVLEKVAQILQTSDRGELLRTGLKVAIVGRPNVGKSSLLNA
WSRSDRAIVTDLPGTTRDVVESQLVVQGIPVQVLDTAGIRDTEDAVEKIGVARSLAASQQADLILFTIDAAVGWTAADQE
IFQRIVATKANQPLILILNKIDIGQPEAIEIPPQVQGVVKTAAAQHQGVDELETAIANLVQAGKVGAADLDFAINQRQAA
ALAQAQQALIQVRETIAQGLPLDFWTIDLRSAIQALGEITGEGVTESVLDRIFSRFCIGK

Sequences:

>Translated_460_residues
MNLGDTIVAIASAVVPNQGSIGIVRLSGAAALPIARQLFQDPGQQTWESHRILYGYVRHPQTQTIVDEALLLLMLAPRSF
TAEDVVEFHCHGGIIPVQQVLQLCLGAGARLATPGEFTLRAFLNGRIDLTQAESVAELVGAKSPQSAQVALAGIQGKLAQ
PIQQLRGQCLDILAEVEARIDFEDDLPPLDEPQIQRDLTQVLEKVAQILQTSDRGELLRTGLKVAIVGRPNVGKSSLLNA
WSRSDRAIVTDLPGTTRDVVESQLVVQGIPVQVLDTAGIRDTEDAVEKIGVARSLAASQQADLILFTIDAAVGWTAADQE
IFQRIVATKANQPLILILNKIDIGQPEAIEIPPQVQGVVKTAAAQHQGVDELETAIANLVQAGKVGAADLDFAINQRQAA
ALAQAQQALIQVRETIAQGLPLDFWTIDLRSAIQALGEITGEGVTESVLDRIFSRFCIGK
>Mature_460_residues
MNLGDTIVAIASAVVPNQGSIGIVRLSGAAALPIARQLFQDPGQQTWESHRILYGYVRHPQTQTIVDEALLLLMLAPRSF
TAEDVVEFHCHGGIIPVQQVLQLCLGAGARLATPGEFTLRAFLNGRIDLTQAESVAELVGAKSPQSAQVALAGIQGKLAQ
PIQQLRGQCLDILAEVEARIDFEDDLPPLDEPQIQRDLTQVLEKVAQILQTSDRGELLRTGLKVAIVGRPNVGKSSLLNA
WSRSDRAIVTDLPGTTRDVVESQLVVQGIPVQVLDTAGIRDTEDAVEKIGVARSLAASQQADLILFTIDAAVGWTAADQE
IFQRIVATKANQPLILILNKIDIGQPEAIEIPPQVQGVVKTAAAQHQGVDELETAIANLVQAGKVGAADLDFAINQRQAA
ALAQAQQALIQVRETIAQGLPLDFWTIDLRSAIQALGEITGEGVTESVLDRIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=472, Percent_Identity=35.3813559322034, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI193082988, Length=505, Percent_Identity=33.4653465346535, Blast_Score=180, Evalue=2e-45,
Organism=Homo sapiens, GI193082993, Length=465, Percent_Identity=34.8387096774194, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI2367268, Length=466, Percent_Identity=35.6223175965665, Blast_Score=248, Evalue=7e-67,
Organism=Escherichia coli, GI87082120, Length=124, Percent_Identity=41.1290322580645, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17507259, Length=468, Percent_Identity=29.7008547008547, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323665, Length=502, Percent_Identity=28.6852589641434, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45550813, Length=489, Percent_Identity=30.4703476482618, Blast_Score=175, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SYNP2 (B1XKC3)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734425.1
- ProteinModelPortal:   B1XKC3
- SMR:   B1XKC3
- GeneID:   6056272
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1170
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49295; Mature: 49295

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: BINDING 25-25 BINDING 87-87 BINDING 126-126 BINDING 460-460

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLGDTIVAIASAVVPNQGSIGIVRLSGAAALPIARQLFQDPGQQTWESHRILYGYVRHP
CCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHCCCEEEEECCCC
QTQTIVDEALLLLMLAPRSFTAEDVVEFHCHGGIIPVQQVLQLCLGAGARLATPGEFTLR
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
AFLNGRIDLTQAESVAELVGAKSPQSAQVALAGIQGKLAQPIQQLRGQCLDILAEVEARI
HHHCCCEEEHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
DFEDDLPPLDEPQIQRDLTQVLEKVAQILQTSDRGELLRTGLKVAIVGRPNVGKSSLLNA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHHHHHH
WSRSDRAIVTDLPGTTRDVVESQLVVQGIPVQVLDTAGIRDTEDAVEKIGVARSLAASQQ
HHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
ADLILFTIDAAVGWTAADQEIFQRIVATKANQPLILILNKIDIGQPEAIEIPPQVQGVVK
CCEEEEEECCHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEECCHHHHHHHH
TAAAQHQGVDELETAIANLVQAGKVGAADLDFAINQRQAAALAQAQQALIQVRETIAQGL
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLDFWTIDLRSAIQALGEITGEGVTESVLDRIFSRFCIGK
CCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLGDTIVAIASAVVPNQGSIGIVRLSGAAALPIARQLFQDPGQQTWESHRILYGYVRHP
CCCCHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHCCCEEEEECCCC
QTQTIVDEALLLLMLAPRSFTAEDVVEFHCHGGIIPVQQVLQLCLGAGARLATPGEFTLR
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHH
AFLNGRIDLTQAESVAELVGAKSPQSAQVALAGIQGKLAQPIQQLRGQCLDILAEVEARI
HHHCCCEEEHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHC
DFEDDLPPLDEPQIQRDLTQVLEKVAQILQTSDRGELLRTGLKVAIVGRPNVGKSSLLNA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEECCCCCHHHHHHH
WSRSDRAIVTDLPGTTRDVVESQLVVQGIPVQVLDTAGIRDTEDAVEKIGVARSLAASQQ
HHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
ADLILFTIDAAVGWTAADQEIFQRIVATKANQPLILILNKIDIGQPEAIEIPPQVQGVVK
CCEEEEEECCHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEECCHHHHHHHH
TAAAQHQGVDELETAIANLVQAGKVGAADLDFAINQRQAAALAQAQQALIQVRETIAQGL
HHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLDFWTIDLRSAIQALGEITGEGVTESVLDRIFSRFCIGK
CCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA