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The map label for this gene is trmH [C]

Identifier: 170077725

GI number: 170077725

Start: 1135208

End: 1135996

Strand: Direct

Name: trmH [C]

Synonym: SYNPCC7002_A1107

Alternate gene names: 170077725

Gene position: 1135208-1135996 (Clockwise)

Preceding gene: 170077723

Following gene: 170077732

Centisome position: 37.74

GC content: 55.01

Gene sequence:

>789_bases
ATGCTCACAAGTTTACAAAATCCACTGGTTAAGGAGATTCGCAAGCTGCACCAAGGGCGGGAACGGCAAAAACAAGGTCG
TTTGTTACTGGAGGGAACCAATCTAATCGAGGCGGCGATCGCCAAGGGATTTTCTTTCCAGACGGTACTGTTTACGGCAG
CTTGGCAGACGAAATATCCGGCACTGGCTAGTCAACTCAACGGCGATCGCCAAACTCTGGTTTCAGAAGAAGTGCTGCAA
AAGGTGGCAACCACGGTGAATCCTGATGGTATTGTGGCAACCCTCGACCAGGCACTCATTTCCCAGACGCCGCCCCAAAC
CCTCCAATTGGGGGTAGCCGTGGAACGACTCCAGGATCCAGGCAACCTGGGGACAATCATTCGTACAGCTACGGCCACGA
CGGTCGATGGTCTGTTGCTGAGTCATGACAGCGTCAATCCCAGTAACCCGAAGGTGCTGCGGGCCTCCGTGGGGGCTTGG
TTCCAAACGACGCCCGTAGTTATCCCCACCTTTGCTGAACAATTGCAACGGTACCGCGCTCAGGGGGTACAGATTGTGGC
CACCCTGCCCCAGGCAACCAAAACCTACTGGGATGTGGATTGGCGACAACCAAGTTTAATTCTATTGGGTAACGAAGGAG
CTGGTTTATCCCCAGCGATCGCCAGTCTCGCCAACGAAACGGTGCAGATTCCCATGGGGCCGGGGGTCGAATCTCTCAAT
GTGGCGATCGCCTGTGCCTTGATGCTCTACGAGGCCCGTCGCCAGCGGGATCACCAAACTTTTTCCTAA

Upstream 100 bases:

>100_bases
CCTTGCGAGTTCAAGTCTCGCGTTCCGCATCTATCCGGCAAAAGTTGTTTGCCTTGCCTGAAGCCTTTTGACGACAACGG
TAAGGTCGATGGGGGCAAAG

Downstream 100 bases:

>100_bases
TTTCCCGACAGTTAGGTTTGGAGCGTCTCCAGTACCGTTTGAGCATGGCCTTTAACCTTCACCTTTGACCAAATTGCCGC
CACTTGACCCTGGGGATCGA

Product: rRNA methylase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLTSLQNPLVKEIRKLHQGRERQKQGRLLLEGTNLIEAAIAKGFSFQTVLFTAAWQTKYPALASQLNGDRQTLVSEEVLQ
KVATTVNPDGIVATLDQALISQTPPQTLQLGVAVERLQDPGNLGTIIRTATATTVDGLLLSHDSVNPSNPKVLRASVGAW
FQTTPVVIPTFAEQLQRYRAQGVQIVATLPQATKTYWDVDWRQPSLILLGNEGAGLSPAIASLANETVQIPMGPGVESLN
VAIACALMLYEARRQRDHQTFS

Sequences:

>Translated_262_residues
MLTSLQNPLVKEIRKLHQGRERQKQGRLLLEGTNLIEAAIAKGFSFQTVLFTAAWQTKYPALASQLNGDRQTLVSEEVLQ
KVATTVNPDGIVATLDQALISQTPPQTLQLGVAVERLQDPGNLGTIIRTATATTVDGLLLSHDSVNPSNPKVLRASVGAW
FQTTPVVIPTFAEQLQRYRAQGVQIVATLPQATKTYWDVDWRQPSLILLGNEGAGLSPAIASLANETVQIPMGPGVESLN
VAIACALMLYEARRQRDHQTFS
>Mature_262_residues
MLTSLQNPLVKEIRKLHQGRERQKQGRLLLEGTNLIEAAIAKGFSFQTVLFTAAWQTKYPALASQLNGDRQTLVSEEVLQ
KVATTVNPDGIVATLDQALISQTPPQTLQLGVAVERLQDPGNLGTIIRTATATTVDGLLLSHDSVNPSNPKVLRASVGAW
FQTTPVVIPTFAEQLQRYRAQGVQIVATLPQATKTYWDVDWRQPSLILLGNEGAGLSPAIASLANETVQIPMGPGVESLN
VAIACALMLYEARRQRDHQTFS

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=291, Percent_Identity=28.8659793814433, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI40068479, Length=163, Percent_Identity=29.4478527607362, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=35.5263157894737, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790623, Length=169, Percent_Identity=26.6272189349112, Blast_Score=69, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=248, Percent_Identity=26.6129032258064, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24666840, Length=293, Percent_Identity=26.6211604095563, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 28499; Mature: 28499

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSLQNPLVKEIRKLHQGRERQKQGRLLLEGTNLIEAAIAKGFSFQTVLFTAAWQTKYP
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHCCCCHHHHEEEHHHCCCCH
ALASQLNGDRQTLVSEEVLQKVATTVNPDGIVATLDQALISQTPPQTLQLGVAVERLQDP
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
GNLGTIIRTATATTVDGLLLSHDSVNPSNPKVLRASVGAWFQTTPVVIPTFAEQLQRYRA
CCCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHH
QGVQIVATLPQATKTYWDVDWRQPSLILLGNEGAGLSPAIASLANETVQIPMGPGVESLN
CCEEEEEECCHHHHHEEECCCCCCCEEEEECCCCCCCHHHHHHCCCEEEECCCCCHHHHH
VAIACALMLYEARRQRDHQTFS
HHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLTSLQNPLVKEIRKLHQGRERQKQGRLLLEGTNLIEAAIAKGFSFQTVLFTAAWQTKYP
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHCCCCHHHHEEEHHHCCCCH
ALASQLNGDRQTLVSEEVLQKVATTVNPDGIVATLDQALISQTPPQTLQLGVAVERLQDP
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
GNLGTIIRTATATTVDGLLLSHDSVNPSNPKVLRASVGAWFQTTPVVIPTFAEQLQRYRA
CCCCHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHH
QGVQIVATLPQATKTYWDVDWRQPSLILLGNEGAGLSPAIASLANETVQIPMGPGVESLN
CCEEEEEECCHHHHHEEECCCCCCCEEEEECCCCCCCHHHHHHCCCEEEECCCCCHHHHH
VAIACALMLYEARRQRDHQTFS
HHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]