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The map label for this gene is prfA

Identifier: 170077656

GI number: 170077656

Start: 1072963

End: 1074054

Strand: Reverse

Name: prfA

Synonym: SYNPCC7002_A1036

Alternate gene names: 170077656

Gene position: 1074054-1072963 (Counterclockwise)

Preceding gene: 170077657

Following gene: 170077654

Centisome position: 35.71

GC content: 50.92

Gene sequence:

>1092_bases
ATGGCTGAAGCATACTTACTCGATAAACTAAAGTCCGTCGAACAAACCTTCCAGGAAATGACCCGACGCCTGGCCGATCC
CGACATCGCGACGAACCCCGATGAGCTACAACGGGTGGCAAAAATTCGCTCCTCCCTCGAAGAAACAGTAAATACCTTCA
ATCAGTGGAAAGAAGCACAAGAAGAACTCCGGGGGGCCAAAGAAATTCTCAAAGAATCCGCTGGAGATCCCGAAATGAAG
GAAATGGCGGCTTTAGAAGTCGAGGAACTCGAAAATACCATTGATACCCTCGAAAATCGTCTGAAGATTCTGCTGTTACC
CAAAGATCCCAACGACGAAAAGAACATCATGTTAGAAATTCGCGCCGGAACAGGGGGCGATGAAGCGAGCATTTGGGCTG
GGGATCTGCTCCGCATGTATTCTCGCTATGCCGAAGGTCAAAACTGGCGGGTGAAACTCCTGAGTGAATCGGCCGCCGAC
ATGGGGGGCTTCAAGGCCGTCATCCTAGAAATTCAAGGGGAACAGGTTTACAGCAAATTAAAATATGAAGCTGGCGTCCA
CCGAGTCCAGCGGGTACCTGTTACCGAAGCTGGGGGGCGGGTTCACACCTCCACAGCAACCATTGCTGTGATGCCAGAAG
TTGATGATGTCGAAGTAGAAATTGATCCTAAGGACATTGAGATGACCACCGCCCGTTCTGGTGGGGCCGGGGGACAAAAC
GTCAACAAAGTAGAAACAGCGGTTGACCTGTTCCACAAGCCGACGGGAATCCGCGTTTTTTGTACAGAAGAGCGATCGCA
ATTACAGAACCGAGAGCGGGCAATGCAAATTCTCCGGGCAAAGCTCTATGAGATGGAACTCCAGCGGCAGCAAGAGGAAG
TCAGTTCCATGCGCAAATCCCAGGTGGGTACCGGCGCCCGTTCTGAAAAAATTCGCACCTACAATTACAAAGATAATCGC
GTCACAGATCACCGCCTAGGCCAAAACTTTTCCTTAGCCCCGGCCTTAGAAGGAGATATTGAGGATATGCTCCAGGCTTG
TATTTCCCAAGACCAACAGGCTCAACTCGAACAACTCGCCGCCGAAAGCTAA

Upstream 100 bases:

>100_bases
GTATTTCCTTGAGTTTTCCCAACCCGGCAGCCCCCTGCTGGGTTCCTTTGTTTTTTAGGGATCTCTTTCCTTCCCCTAGC
GTAAAAACGTTCTGTGCATT

Downstream 100 bases:

>100_bases
TAAATCCTTCCCTGGGGAAGGTGGCCGCAGGCCGGAAGGGGTTCTTCCCTGGGAAAGGCGATCGCCGCTCGGAAGAAGTT
CCTTCCCTATGGTTGAACCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAEAYLLDKLKSVEQTFQEMTRRLADPDIATNPDELQRVAKIRSSLEETVNTFNQWKEAQEELRGAKEILKESAGDPEMK
EMAALEVEELENTIDTLENRLKILLLPKDPNDEKNIMLEIRAGTGGDEASIWAGDLLRMYSRYAEGQNWRVKLLSESAAD
MGGFKAVILEIQGEQVYSKLKYEAGVHRVQRVPVTEAGGRVHTSTATIAVMPEVDDVEVEIDPKDIEMTTARSGGAGGQN
VNKVETAVDLFHKPTGIRVFCTEERSQLQNRERAMQILRAKLYEMELQRQQEEVSSMRKSQVGTGARSEKIRTYNYKDNR
VTDHRLGQNFSLAPALEGDIEDMLQACISQDQQAQLEQLAAES

Sequences:

>Translated_363_residues
MAEAYLLDKLKSVEQTFQEMTRRLADPDIATNPDELQRVAKIRSSLEETVNTFNQWKEAQEELRGAKEILKESAGDPEMK
EMAALEVEELENTIDTLENRLKILLLPKDPNDEKNIMLEIRAGTGGDEASIWAGDLLRMYSRYAEGQNWRVKLLSESAAD
MGGFKAVILEIQGEQVYSKLKYEAGVHRVQRVPVTEAGGRVHTSTATIAVMPEVDDVEVEIDPKDIEMTTARSGGAGGQN
VNKVETAVDLFHKPTGIRVFCTEERSQLQNRERAMQILRAKLYEMELQRQQEEVSSMRKSQVGTGARSEKIRTYNYKDNR
VTDHRLGQNFSLAPALEGDIEDMLQACISQDQQAQLEQLAAES
>Mature_362_residues
AEAYLLDKLKSVEQTFQEMTRRLADPDIATNPDELQRVAKIRSSLEETVNTFNQWKEAQEELRGAKEILKESAGDPEMKE
MAALEVEELENTIDTLENRLKILLLPKDPNDEKNIMLEIRAGTGGDEASIWAGDLLRMYSRYAEGQNWRVKLLSESAADM
GGFKAVILEIQGEQVYSKLKYEAGVHRVQRVPVTEAGGRVHTSTATIAVMPEVDDVEVEIDPKDIEMTTARSGGAGGQNV
NKVETAVDLFHKPTGIRVFCTEERSQLQNRERAMQILRAKLYEMELQRQQEEVSSMRKSQVGTGARSEKIRTYNYKDNRV
TDHRLGQNFSLAPALEGDIEDMLQACISQDQQAQLEQLAAES

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=292, Percent_Identity=40.4109589041096, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI34577120, Length=298, Percent_Identity=39.9328859060403, Blast_Score=228, Evalue=7e-60,
Organism=Homo sapiens, GI166795305, Length=198, Percent_Identity=37.8787878787879, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=43.8888888888889, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=37.5, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=302, Percent_Identity=38.0794701986755, Blast_Score=199, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=38.4848484848485, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI19921226, Length=331, Percent_Identity=38.0664652567976, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_SYNP2 (B1XJH8)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734294.1
- ProteinModelPortal:   B1XJH8
- SMR:   B1XJH8
- GeneID:   6057719
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1036
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40893; Mature: 40761

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAYLLDKLKSVEQTFQEMTRRLADPDIATNPDELQRVAKIRSSLEETVNTFNQWKEAQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRGAKEILKESAGDPEMKEMAALEVEELENTIDTLENRLKILLLPKDPNDEKNIMLEI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEE
RAGTGGDEASIWAGDLLRMYSRYAEGQNWRVKLLSESAADMGGFKAVILEIQGEQVYSKL
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCEEEEEEECCHHHHHHH
KYEAGVHRVQRVPVTEAGGRVHTSTATIAVMPEVDDVEVEIDPKDIEMTTARSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCC
VNKVETAVDLFHKPTGIRVFCTEERSQLQNRERAMQILRAKLYEMELQRQQEEVSSMRKS
HHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGARSEKIRTYNYKDNRVTDHRLGQNFSLAPALEGDIEDMLQACISQDQQAQLEQLA
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
AES
CCC
>Mature Secondary Structure 
AEAYLLDKLKSVEQTFQEMTRRLADPDIATNPDELQRVAKIRSSLEETVNTFNQWKEAQ
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRGAKEILKESAGDPEMKEMAALEVEELENTIDTLENRLKILLLPKDPNDEKNIMLEI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEE
RAGTGGDEASIWAGDLLRMYSRYAEGQNWRVKLLSESAADMGGFKAVILEIQGEQVYSKL
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHCCCCCCEEEEEEECCHHHHHHH
KYEAGVHRVQRVPVTEAGGRVHTSTATIAVMPEVDDVEVEIDPKDIEMTTARSGGAGGQN
HHHHHHHHHHCCCCCCCCCEEEECCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCC
VNKVETAVDLFHKPTGIRVFCTEERSQLQNRERAMQILRAKLYEMELQRQQEEVSSMRKS
HHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVGTGARSEKIRTYNYKDNRVTDHRLGQNFSLAPALEGDIEDMLQACISQDQQAQLEQLA
HCCCCCCCCCEEEECCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
AES
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA