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The map label for this gene is potF [H]

Identifier: 170076941

GI number: 170076941

Start: 326934

End: 328067

Strand: Direct

Name: potF [H]

Synonym: SYNPCC7002_A0313

Alternate gene names: 170076941

Gene position: 326934-328067 (Clockwise)

Preceding gene: 170076939

Following gene: 170076942

Centisome position: 10.87

GC content: 52.73

Gene sequence:

>1134_bases
ATGCCCAATCGCCGCCATTTTTTGACTATTTCCGCACTCTATGCCCTCGCTGGGTGTTTGGGAGGGTGTCAACGAACCTC
GGAGACAACTTTACAAATCAATACATTACGGGGGTCAATTTCGCCACAACTGTTGACATTGTTTCGGCAAAACCAAGGGC
AAACGGTCAGCTTAGATCTCAAACCCCTAGAACAGCTCCAAGAATTATTTGTAGTGCTGCAGGATGCGGCCATGAGGATA
CCGGAGACATCTGAAAATTTCCTCAATCGCTCGGCAGCGGTGCCGGATTTGCTGACCCTGGGCCATGGTTGGTATCAACA
GGCGATCGCCTTGGGATTTGTTCAACCGTTATCTCTGGAGGAACTAACGCGGTGGGAAGAGTTGGGGGAACCTTGGCAAC
GGTTTTTGCGCCGCAATGTCCAGGGCGAGATCGACCCCCAAGGGCAACTGTGGGGACAGCCCTACCGCTGGGGCACGACG
ATGCTGGTCTACCGCAAGGATAAGTTGAAAAACCTGGGGTGGACACCGACGGATTGGGCAGATCTATGGCGGCCAGAATT
ACAGGGAAAAATTTCTCTGCTGAATCAATCGCGGGAAGTGTTGGGGCTTTTGCTGAAAAAACAGGGGAAATCCTACAACG
AAACCGATCCAGAGGCGATCGCCAATTTAGAGAGCGATTTCCAAACCCTCCACCGCCAGGTCAAATTCTATAGTTCCACC
CACTATCTCCAGCCCCTCAACATGGGCGACACCTGGATTGCCCAGGGGTGGTCCCAGGATATTTTGCCGCTCCTGCAACG
CTACCGCAATTTAGGGGCAGTGATTCCCGCCAGTGGCACGGCCCTCTGGTGTGATCTGTGGGTGCAGCCCCAACGCAGCC
AAACTTCTTTTGAGACCCTAAAAACTTGGTTAGATTTTTGTTGGGAACCGGCGGCGATTCAGCAGATTTCCCGTAGTAGT
CACGGGGCCTCTCCACTGATTTATGCGGCGACCAATTTAGACCCTGCCATCACTGATAATCCGTTAATTTACCTTGATCA
AAACACCTTCCGGGCCAGTGAAATCATTGAGATGTTAGACCCCGACGCGACGGCCCAGTATCAACAGGTTTGGCAAGCAG
TGCGCCAGGGCTAG

Upstream 100 bases:

>100_bases
GATAAAGTTGCAATTGTCCTGGCCAAGGTTGAACTTGATTAAAGGCTAAATTTGCTATTTTTTGAAAAGATATTTCCCAG
GGGAAACGACATTTAGATTT

Downstream 100 bases:

>100_bases
GCCAGAAAAATTTTGATTTTGCCCACCGCTGTTCTGGATAATGCGGAGGAAGATCATCGATTGAGGAGTTCCCATGACCC
ACGACACCATTGGGGTGGCG

Product: periplasmic binding protein of ABC transporter

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MPNRRHFLTISALYALAGCLGGCQRTSETTLQINTLRGSISPQLLTLFRQNQGQTVSLDLKPLEQLQELFVVLQDAAMRI
PETSENFLNRSAAVPDLLTLGHGWYQQAIALGFVQPLSLEELTRWEELGEPWQRFLRRNVQGEIDPQGQLWGQPYRWGTT
MLVYRKDKLKNLGWTPTDWADLWRPELQGKISLLNQSREVLGLLLKKQGKSYNETDPEAIANLESDFQTLHRQVKFYSST
HYLQPLNMGDTWIAQGWSQDILPLLQRYRNLGAVIPASGTALWCDLWVQPQRSQTSFETLKTWLDFCWEPAAIQQISRSS
HGASPLIYAATNLDPAITDNPLIYLDQNTFRASEIIEMLDPDATAQYQQVWQAVRQG

Sequences:

>Translated_377_residues
MPNRRHFLTISALYALAGCLGGCQRTSETTLQINTLRGSISPQLLTLFRQNQGQTVSLDLKPLEQLQELFVVLQDAAMRI
PETSENFLNRSAAVPDLLTLGHGWYQQAIALGFVQPLSLEELTRWEELGEPWQRFLRRNVQGEIDPQGQLWGQPYRWGTT
MLVYRKDKLKNLGWTPTDWADLWRPELQGKISLLNQSREVLGLLLKKQGKSYNETDPEAIANLESDFQTLHRQVKFYSST
HYLQPLNMGDTWIAQGWSQDILPLLQRYRNLGAVIPASGTALWCDLWVQPQRSQTSFETLKTWLDFCWEPAAIQQISRSS
HGASPLIYAATNLDPAITDNPLIYLDQNTFRASEIIEMLDPDATAQYQQVWQAVRQG
>Mature_376_residues
PNRRHFLTISALYALAGCLGGCQRTSETTLQINTLRGSISPQLLTLFRQNQGQTVSLDLKPLEQLQELFVVLQDAAMRIP
ETSENFLNRSAAVPDLLTLGHGWYQQAIALGFVQPLSLEELTRWEELGEPWQRFLRRNVQGEIDPQGQLWGQPYRWGTTM
LVYRKDKLKNLGWTPTDWADLWRPELQGKISLLNQSREVLGLLLKKQGKSYNETDPEAIANLESDFQTLHRQVKFYSSTH
YLQPLNMGDTWIAQGWSQDILPLLQRYRNLGAVIPASGTALWCDLWVQPQRSQTSFETLKTWLDFCWEPAAIQQISRSSH
GASPLIYAATNLDPAITDNPLIYLDQNTFRASEIIEMLDPDATAQYQQVWQAVRQG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=384, Percent_Identity=22.9166666666667, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1787367, Length=266, Percent_Identity=24.4360902255639, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 43071; Mature: 42940

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNRRHFLTISALYALAGCLGGCQRTSETTLQINTLRGSISPQLLTLFRQNQGQTVSLDL
CCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEC
KPLEQLQELFVVLQDAAMRIPETSENFLNRSAAVPDLLTLGHGWYQQAIALGFVQPLSLE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCCHH
ELTRWEELGEPWQRFLRRNVQGEIDPQGQLWGQPYRWGTTMLVYRKDKLKNLGWTPTDWA
HHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCHH
DLWRPELQGKISLLNQSREVLGLLLKKQGKSYNETDPEAIANLESDFQTLHRQVKFYSST
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
HYLQPLNMGDTWIAQGWSQDILPLLQRYRNLGAVIPASGTALWCDLWVQPQRSQTSFETL
CEECCCCCCCHHHHCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEEEECCCCCCHHHHHH
KTWLDFCWEPAAIQQISRSSHGASPLIYAATNLDPAITDNPLIYLDQNTFRASEIIEMLD
HHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHC
PDATAQYQQVWQAVRQG
CCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNRRHFLTISALYALAGCLGGCQRTSETTLQINTLRGSISPQLLTLFRQNQGQTVSLDL
CCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEC
KPLEQLQELFVVLQDAAMRIPETSENFLNRSAAVPDLLTLGHGWYQQAIALGFVQPLSLE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCCHH
ELTRWEELGEPWQRFLRRNVQGEIDPQGQLWGQPYRWGTTMLVYRKDKLKNLGWTPTDWA
HHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCHH
DLWRPELQGKISLLNQSREVLGLLLKKQGKSYNETDPEAIANLESDFQTLHRQVKFYSST
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
HYLQPLNMGDTWIAQGWSQDILPLLQRYRNLGAVIPASGTALWCDLWVQPQRSQTSFETL
CEECCCCCCCHHHHCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEEEECCCCCCHHHHHH
KTWLDFCWEPAAIQQISRSSHGASPLIYAATNLDPAITDNPLIYLDQNTFRASEIIEMLD
HHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHC
PDATAQYQQVWQAVRQG
CCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]