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The map label for this gene is birA [H]

Identifier: 170076718

GI number: 170076718

Start: 86991

End: 87764

Strand: Direct

Name: birA [H]

Synonym: SYNPCC7002_A0084

Alternate gene names: 170076718

Gene position: 86991-87764 (Clockwise)

Preceding gene: 170076712

Following gene: 170076719

Centisome position: 2.89

GC content: 49.61

Gene sequence:

>774_bases
GTGTTCCATTCTGACGCGATTCAAGGGGCCTTGAGTGATCTTCTTGCCTGTGAAACGACACTTCCCATCGAAGTTTTCCC
GGAGCTTGACTCGACCAACGCGCTCCTGTGGCAACGGTATCAAGCACAATGCACAATGCCTAGGGTGGCGATCGCCTCAA
CCCAGACAGCGGGGCGGGGCCAGTGGGGACGCACCTGGATTTCTGCTCCGGGGGGACTCTACTTATCAGTTTTGTTGCCC
ATTGAATTAGAGCCTCAATTAGCCTATGGCTTAACCCTAGCCAGCGTTTGGGGCATTGTTGAAATGTTCCAACAGCAAGG
GCTTCCCGTACAAATTAAATGGCCCAATGATCTTCTCTTGCAAAACAAAAAACTGGGGGGCATTAAAACCGAAACGAAAG
TCTCCCAGGGGAAAATTAGTGCGGCTGTGGTCGGGGTCGGCATTAACTACCGAAACCCAGTTCCTCCAACGGGCATTAAT
CTCGAACAATTCTGGCAAGGGGAGTTGAAAATTAAAGGCGATCGCCTTGCGGCGTTGGTTATTGCCGGAATTCTGCAGGG
GACGCAAAAACTAGCTCGAAATTCAATTGAAAATATATTGCCGGGCTATTTAAAGCACCTCAAAAACCTAGGCGAAATAA
TCACCTATGAAGGCCATCAAGGGATCATTACCGGGGTCAATCAAAAGGGCGAACTACTGGTTAAACTTAGCGCTCCCGGT
GCCAGTAGCATTGTAAAAATTCCCCCTGGTCAAATTTCCCTCGGCTATCTGTAG

Upstream 100 bases:

>100_bases
TCCGAAAAAGAACGCTGGCGTTTCACTTTAGCAAATTCCTGGGGTGAGGAACGCTGCAAGGTACGCTATTTGAGAAAAGC
GATAAAAATATTTACTGACT

Downstream 100 bases:

>100_bases
AGAATCTCGTTTTAAATTTGACTTTCCCAATAGAGCTTGCCACACTGCAAATCGTTGTCTCAGAACAAATATTCAAGAAT
ATGTCAACCTATCTCGTTAC

Product: biotin-acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MFHSDAIQGALSDLLACETTLPIEVFPELDSTNALLWQRYQAQCTMPRVAIASTQTAGRGQWGRTWISAPGGLYLSVLLP
IELEPQLAYGLTLASVWGIVEMFQQQGLPVQIKWPNDLLLQNKKLGGIKTETKVSQGKISAAVVGVGINYRNPVPPTGIN
LEQFWQGELKIKGDRLAALVIAGILQGTQKLARNSIENILPGYLKHLKNLGEIITYEGHQGIITGVNQKGELLVKLSAPG
ASSIVKIPPGQISLGYL

Sequences:

>Translated_257_residues
MFHSDAIQGALSDLLACETTLPIEVFPELDSTNALLWQRYQAQCTMPRVAIASTQTAGRGQWGRTWISAPGGLYLSVLLP
IELEPQLAYGLTLASVWGIVEMFQQQGLPVQIKWPNDLLLQNKKLGGIKTETKVSQGKISAAVVGVGINYRNPVPPTGIN
LEQFWQGELKIKGDRLAALVIAGILQGTQKLARNSIENILPGYLKHLKNLGEIITYEGHQGIITGVNQKGELLVKLSAPG
ASSIVKIPPGQISLGYL
>Mature_257_residues
MFHSDAIQGALSDLLACETTLPIEVFPELDSTNALLWQRYQAQCTMPRVAIASTQTAGRGQWGRTWISAPGGLYLSVLLP
IELEPQLAYGLTLASVWGIVEMFQQQGLPVQIKWPNDLLLQNKKLGGIKTETKVSQGKISAAVVGVGINYRNPVPPTGIN
LEQFWQGELKIKGDRLAALVIAGILQGTQKLARNSIENILPGYLKHLKNLGEIITYEGHQGIITGVNQKGELLVKLSAPG
ASSIVKIPPGQISLGYL

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=229, Percent_Identity=25.3275109170306, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1790408, Length=228, Percent_Identity=34.6491228070175, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR013196
- InterPro:   IPR008988
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB; PF08279 HTH_11 [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27813; Mature: 27813

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHSDAIQGALSDLLACETTLPIEVFPELDSTNALLWQRYQAQCTMPRVAIASTQTAGRG
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
QWGRTWISAPGGLYLSVLLPIELEPQLAYGLTLASVWGIVEMFQQQGLPVQIKWPNDLLL
CCCCEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHEE
QNKKLGGIKTETKVSQGKISAAVVGVGINYRNPVPPTGINLEQFWQGELKIKGDRLAALV
ECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCCCEEECCCHHHHHH
IAGILQGTQKLARNSIENILPGYLKHLKNLGEIITYEGHQGIITGVNQKGELLVKLSAPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCEEEEEECCC
ASSIVKIPPGQISLGYL
CCCEEECCCCCEEECCC
>Mature Secondary Structure
MFHSDAIQGALSDLLACETTLPIEVFPELDSTNALLWQRYQAQCTMPRVAIASTQTAGRG
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCC
QWGRTWISAPGGLYLSVLLPIELEPQLAYGLTLASVWGIVEMFQQQGLPVQIKWPNDLLL
CCCCEEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHEE
QNKKLGGIKTETKVSQGKISAAVVGVGINYRNPVPPTGINLEQFWQGELKIKGDRLAALV
ECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHCCCCEEECCCHHHHHH
IAGILQGTQKLARNSIENILPGYLKHLKNLGEIITYEGHQGIITGVNQKGELLVKLSAPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCEEEEEECCC
ASSIVKIPPGQISLGYL
CCCEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3899863; 3536662; 8265357; 9278503; 1311302; 1328157; 1409631; 11353844 [H]