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The map label for this gene is relA [H]

Identifier: 170025664

GI number: 170025664

Start: 3783755

End: 3785989

Strand: Reverse

Name: relA [H]

Synonym: YPK_3449

Alternate gene names: 170025664

Gene position: 3785989-3783755 (Counterclockwise)

Preceding gene: 170025665

Following gene: 170025663

Centisome position: 80.73

GC content: 50.65

Gene sequence:

>2235_bases
ATGGTTGCGGTACGAAGTGCGCATTTAAACCCAGCGGGTGAGTTTGCTCTTGACGATTGGATTGCCAGTTTAGGGCTTGC
CAATCCGCAGTCATCTGAGCGATTAGCCGAAACCTGGCGTTACTGTGAACAACAGACGCAAAACCATCCCGATGCCTCGT
TATTACTGTGGCGCGGTCTTGAAATGGTTGAAATTCTTTCCACTCTGAGTATGGATAACGACAGTATGCGCGCGGCGCTG
CTGTTCCCGCTGGTAGACGCCAATATCGTGGACGAAACTACCCTGACCGAGCAATTCGGTAAGGGGATTACGTATCTGGT
ACACGGTGTGCGTGATATGGATGCTATCCGCCAGTTAAAAGCCACTCACAACGATTCCATGAGCTCGGAGCAAGTGGATA
ACGTGCGCCGTATGTTATTGGCGATGGTGGAGGATTTCCGCTGCGTGGTGATCAAGCTGGCAGAGCGGATCGCTCATCTG
CGTGAAGTCAAAGATGCACCGGAAGATGAACGTGTTCTGGCGGCTAAAGAGTGTTCCAATATTTACGCACCACTGGCGAA
CCGTTTGGGGATTGGTCAGATTAAATGGGAGTTGGAGGATTTCTGCTTCCGTTATCTGCATCCAGATGAATATAAGAAAA
TCGCCAAATTACTCCACGAGAGGCGTATTGATCGCGAACAATTTATTGATGACTTCGTTGCTTCATTACATAAGGCGATG
GCTGACGAAGGCATTAAAGCCGACATTTATGGCCGACCGAAGCATATCTACAGCATTTGGCGCAAAATGCAGAAAAAGTC
GCTGGCCTTCGATGAGCTATTCGACGTCCGCGCGGTGCGGGTGGTGGTGGAACGCTTGCAGGATTGTTATGCCGCGTTAG
GGATTGTTCACACGCACTTCCGGCATTTACCTGATGAATTTGATGATTATGTGGCCAACCCTAAGCCAAATGGTTACCAA
TCGATCCATACCGTGGTGCTTGGGCCACGAGGTAAAACGTTAGAGATTCAGATCCGTACCCGCCAGATGCATGAAGATGC
CGAGTTGGGGGTTGCTGCGCACTGGAAATATAAAGAAGGGGCCGTGGCAGCGGGCCGTTCGAGTTATGAAGGGCGAATTG
CCTGGTTACGTAAATTGATCGCCTGGCAGGAAGAGATGGCTGATTCTGGCGAAATGCTGGATGAAGTGCGCAGCCAGGTA
TTTGATGATCGGGTGTACGTGTTTACACCGAAAGGCGATGTGATTGACCTACCTGCCGGCTCGACGCCGCTGGATTTTGC
CTACCATATTCATAGCGATGTGGGCCACCGTTGTATCGGTGCCAAAATCAGTGGCCGCATTGTGCCGTTTACTTATCAGT
TGCAGATGGGTGATCAAATTGAAATTATCACTCAAAAACAGCCAAACCCAAGCCGTGACTGGCTGAATCCTAACCTCGGT
TACATCACGACCAGTCGCGGACGTTCCAAAATTCATAACTGGTTCCGCAAACAAGATAGAGATAAGAATATTCTGGCTGG
CCGGCAAATGCTGGATGATGAGCTGGAGCATCTGGATATCAGTCTGAAAGAGGCTGAGAAGCTGCTTGTTCCGCGTTACA
ACATGAATTCGATGGATGAAGTGTTAGCGGCGATTGGGGGGGGTGATATCCGTCTGAATCAGATGGTGAACTTCCTCCAG
GGCAAACTGAATAAACCAACGGCAGAAGAGGCTGATTTAGAAGCACTCCGTCATCTGAATAACAAGCATCAGCCCGCGCC
ACGTAACGCCAGCAAAGACAGTGGGCGTATTGTGGTCGAAGGTGTCGGTAACCTGATGCACCATATTGCCCGTTGTTGCC
AGCCGATCCCCGGTGATCAAATTATCGGTTTTATCACGCAAGGGCGGGGGATATCGATTCACCGTGCAGATTGTGAGCAG
TTGGCTGAATTGGAATCCCATGCACCGGAACGGATTGTCGATGCAGTCTGGGGCGAAAGCTACTCCAGCGGTTATTCGCT
GGTGGTGCGGGTTACGGCTAATGACCGCAGTGGGTTATTGCGCGATATCACTACGATCCTGGCAAATGAGAAAGTTAACG
TACTGGGCGTTGCCAGCCGCAGCGATACCAAAAAGATGGTGGCGACTATCGATATGGATATCGAGATCTATAACTTGCAG
GTGCTGGGCCGGGTATTGGCCAAACTGAATCAGTTGCCGGATGTCATAGACGCCCGTCGCCTCCATGGCAATTAG

Upstream 100 bases:

>100_bases
TGCTCAGGTGCGTACGTTGGATATGTTCCCGCACACCGGGCATCTTGAATCTATGGCGCTGTTTATTCAGGCGTCAGAGG
TCGCAAAGTAGGGAGAAGTT

Downstream 100 bases:

>100_bases
GCTTTTTAATCTGGACGGCGATTACGTTTTGCTCTATTGATCTTGCGCTTTGCTCTATTGGTTACGCTTTGCTCTATTGA
TCTTGCGCGGTGCTCGCCCT

Product: GDP/GTP pyrophosphokinase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MVAVRSAHLNPAGEFALDDWIASLGLANPQSSERLAETWRYCEQQTQNHPDASLLLWRGLEMVEILSTLSMDNDSMRAAL
LFPLVDANIVDETTLTEQFGKGITYLVHGVRDMDAIRQLKATHNDSMSSEQVDNVRRMLLAMVEDFRCVVIKLAERIAHL
REVKDAPEDERVLAAKECSNIYAPLANRLGIGQIKWELEDFCFRYLHPDEYKKIAKLLHERRIDREQFIDDFVASLHKAM
ADEGIKADIYGRPKHIYSIWRKMQKKSLAFDELFDVRAVRVVVERLQDCYAALGIVHTHFRHLPDEFDDYVANPKPNGYQ
SIHTVVLGPRGKTLEIQIRTRQMHEDAELGVAAHWKYKEGAVAAGRSSYEGRIAWLRKLIAWQEEMADSGEMLDEVRSQV
FDDRVYVFTPKGDVIDLPAGSTPLDFAYHIHSDVGHRCIGAKISGRIVPFTYQLQMGDQIEIITQKQPNPSRDWLNPNLG
YITTSRGRSKIHNWFRKQDRDKNILAGRQMLDDELEHLDISLKEAEKLLVPRYNMNSMDEVLAAIGGGDIRLNQMVNFLQ
GKLNKPTAEEADLEALRHLNNKHQPAPRNASKDSGRIVVEGVGNLMHHIARCCQPIPGDQIIGFITQGRGISIHRADCEQ
LAELESHAPERIVDAVWGESYSSGYSLVVRVTANDRSGLLRDITTILANEKVNVLGVASRSDTKKMVATIDMDIEIYNLQ
VLGRVLAKLNQLPDVIDARRLHGN

Sequences:

>Translated_744_residues
MVAVRSAHLNPAGEFALDDWIASLGLANPQSSERLAETWRYCEQQTQNHPDASLLLWRGLEMVEILSTLSMDNDSMRAAL
LFPLVDANIVDETTLTEQFGKGITYLVHGVRDMDAIRQLKATHNDSMSSEQVDNVRRMLLAMVEDFRCVVIKLAERIAHL
REVKDAPEDERVLAAKECSNIYAPLANRLGIGQIKWELEDFCFRYLHPDEYKKIAKLLHERRIDREQFIDDFVASLHKAM
ADEGIKADIYGRPKHIYSIWRKMQKKSLAFDELFDVRAVRVVVERLQDCYAALGIVHTHFRHLPDEFDDYVANPKPNGYQ
SIHTVVLGPRGKTLEIQIRTRQMHEDAELGVAAHWKYKEGAVAAGRSSYEGRIAWLRKLIAWQEEMADSGEMLDEVRSQV
FDDRVYVFTPKGDVIDLPAGSTPLDFAYHIHSDVGHRCIGAKISGRIVPFTYQLQMGDQIEIITQKQPNPSRDWLNPNLG
YITTSRGRSKIHNWFRKQDRDKNILAGRQMLDDELEHLDISLKEAEKLLVPRYNMNSMDEVLAAIGGGDIRLNQMVNFLQ
GKLNKPTAEEADLEALRHLNNKHQPAPRNASKDSGRIVVEGVGNLMHHIARCCQPIPGDQIIGFITQGRGISIHRADCEQ
LAELESHAPERIVDAVWGESYSSGYSLVVRVTANDRSGLLRDITTILANEKVNVLGVASRSDTKKMVATIDMDIEIYNLQ
VLGRVLAKLNQLPDVIDARRLHGN
>Mature_744_residues
MVAVRSAHLNPAGEFALDDWIASLGLANPQSSERLAETWRYCEQQTQNHPDASLLLWRGLEMVEILSTLSMDNDSMRAAL
LFPLVDANIVDETTLTEQFGKGITYLVHGVRDMDAIRQLKATHNDSMSSEQVDNVRRMLLAMVEDFRCVVIKLAERIAHL
REVKDAPEDERVLAAKECSNIYAPLANRLGIGQIKWELEDFCFRYLHPDEYKKIAKLLHERRIDREQFIDDFVASLHKAM
ADEGIKADIYGRPKHIYSIWRKMQKKSLAFDELFDVRAVRVVVERLQDCYAALGIVHTHFRHLPDEFDDYVANPKPNGYQ
SIHTVVLGPRGKTLEIQIRTRQMHEDAELGVAAHWKYKEGAVAAGRSSYEGRIAWLRKLIAWQEEMADSGEMLDEVRSQV
FDDRVYVFTPKGDVIDLPAGSTPLDFAYHIHSDVGHRCIGAKISGRIVPFTYQLQMGDQIEIITQKQPNPSRDWLNPNLG
YITTSRGRSKIHNWFRKQDRDKNILAGRQMLDDELEHLDISLKEAEKLLVPRYNMNSMDEVLAAIGGGDIRLNQMVNFLQ
GKLNKPTAEEADLEALRHLNNKHQPAPRNASKDSGRIVVEGVGNLMHHIARCCQPIPGDQIIGFITQGRGISIHRADCEQ
LAELESHAPERIVDAVWGESYSSGYSLVVRVTANDRSGLLRDITTILANEKVNVLGVASRSDTKKMVATIDMDIEIYNLQ
VLGRVLAKLNQLPDVIDARRLHGN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=745, Percent_Identity=84.2953020134228, Blast_Score=1314, Evalue=0.0,
Organism=Escherichia coli, GI1790082, Length=683, Percent_Identity=31.3323572474378, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 84319; Mature: 84319

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAVRSAHLNPAGEFALDDWIASLGLANPQSSERLAETWRYCEQQTQNHPDASLLLWRGL
CCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCH
EMVEILSTLSMDNDSMRAALLFPLVDANIVDETTLTEQFGKGITYLVHGVRDMDAIRQLK
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ATHNDSMSSEQVDNVRRMLLAMVEDFRCVVIKLAERIAHLREVKDAPEDERVLAAKECSN
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IYAPLANRLGIGQIKWELEDFCFRYLHPDEYKKIAKLLHERRIDREQFIDDFVASLHKAM
HHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ADEGIKADIYGRPKHIYSIWRKMQKKSLAFDELFDVRAVRVVVERLQDCYAALGIVHTHF
HCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLPDEFDDYVANPKPNGYQSIHTVVLGPRGKTLEIQIRTRQMHEDAELGVAAHWKYKEG
HHCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEHHHHCCHHCCCEEEEEEECCC
AVAAGRSSYEGRIAWLRKLIAWQEEMADSGEMLDEVRSQVFDDRVYVFTPKGDVIDLPAG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCC
STPLDFAYHIHSDVGHRCIGAKISGRIVPFTYQLQMGDQIEIITQKQPNPSRDWLNPNLG
CCCCHHHEEHHHCCCCEEECCEECCEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCC
YITTSRGRSKIHNWFRKQDRDKNILAGRQMLDDELEHLDISLKEAEKLLVPRYNMNSMDE
EEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHH
VLAAIGGGDIRLNQMVNFLQGKLNKPTAEEADLEALRHLNNKHQPAPRNASKDSGRIVVE
HHHHHCCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEH
GVGNLMHHIARCCQPIPGDQIIGFITQGRGISIHRADCEQLAELESHAPERIVDAVWGES
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHCCC
YSSGYSLVVRVTANDRSGLLRDITTILANEKVNVLGVASRSDTKKMVATIDMDIEIYNLQ
CCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHEEEEEECCEEEEHHH
VLGRVLAKLNQLPDVIDARRLHGN
HHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MVAVRSAHLNPAGEFALDDWIASLGLANPQSSERLAETWRYCEQQTQNHPDASLLLWRGL
CCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHCCH
EMVEILSTLSMDNDSMRAALLFPLVDANIVDETTLTEQFGKGITYLVHGVRDMDAIRQLK
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ATHNDSMSSEQVDNVRRMLLAMVEDFRCVVIKLAERIAHLREVKDAPEDERVLAAKECSN
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
IYAPLANRLGIGQIKWELEDFCFRYLHPDEYKKIAKLLHERRIDREQFIDDFVASLHKAM
HHHHHHHCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
ADEGIKADIYGRPKHIYSIWRKMQKKSLAFDELFDVRAVRVVVERLQDCYAALGIVHTHF
HCCCCEEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHLPDEFDDYVANPKPNGYQSIHTVVLGPRGKTLEIQIRTRQMHEDAELGVAAHWKYKEG
HHCCHHHHHHCCCCCCCCCCEEEEEEECCCCCEEEEEEEHHHHCCHHCCCEEEEEEECCC
AVAAGRSSYEGRIAWLRKLIAWQEEMADSGEMLDEVRSQVFDDRVYVFTPKGDVIDLPAG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCCCEEECCCC
STPLDFAYHIHSDVGHRCIGAKISGRIVPFTYQLQMGDQIEIITQKQPNPSRDWLNPNLG
CCCCHHHEEHHHCCCCEEECCEECCEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCC
YITTSRGRSKIHNWFRKQDRDKNILAGRQMLDDELEHLDISLKEAEKLLVPRYNMNSMDE
EEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHH
VLAAIGGGDIRLNQMVNFLQGKLNKPTAEEADLEALRHLNNKHQPAPRNASKDSGRIVVE
HHHHHCCCCEEHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEH
GVGNLMHHIARCCQPIPGDQIIGFITQGRGISIHRADCEQLAELESHAPERIVDAVWGES
HHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHCCC
YSSGYSLVVRVTANDRSGLLRDITTILANEKVNVLGVASRSDTKKMVATIDMDIEIYNLQ
CCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHEEEEEECCEEEEHHH
VLGRVLAKLNQLPDVIDARRLHGN
HHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]