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The map label for this gene is truD [H]

Identifier: 170025644

GI number: 170025644

Start: 3762759

End: 3763808

Strand: Reverse

Name: truD [H]

Synonym: YPK_3429

Alternate gene names: 170025644

Gene position: 3763808-3762759 (Counterclockwise)

Preceding gene: 170025645

Following gene: 170025643

Centisome position: 80.26

GC content: 49.81

Gene sequence:

>1050_bases
ATGGATATGGAAAACCTGACCTGGCTACACGGTAAACCTACGGCGAGCGGTATTTTAAAAGCGAATCCGGAAGATTTTGT
TGTCGTAGAGGATTTGGGATTTGAACCGGATGGGGAAGGTGAGCATCTGCTGGTTCGTATTCGTAAAAATGGCTGCAATA
CCCAATTCGTTGCTGATTATTTGGCGCGCTTTGCCAAACTTCATCCGCGGTTGGTGAGTTATGCGGGGTTAAAAGACCGC
CATGCGGTCACCGAACAGTGGTTTTGTTTACACTTGCCAGGAAAAGAAGCGCCAGATCTGGCCACCTTTGAGCTGGAAGG
GTGTGAAGTATTGGAAGCGGTGCGGCATAAAAGAAAATTACGCATTGGTTCACTGAAAGGCAATGCGTTTACCTTAGTAC
TGCGCCATATTACGGATCGCCAGGATGTGGAACAACGTTTACAGCAAATAGCCGCCCAGGGCGTACCCAACTATTTTGGT
AGCCAGCGGTTTGGCCGTGGTGGTAATAATCTGGTGCAGGCCCGTTTATGGGCGAATAATGAAATTCGGGTAAAAGAACG
CAGCAAACGTAGCTTTTATCTGTCGGCCAGTCGCAGCGCGATGTTCAATCTGATTAGCAGTCACCGCTTGGCGCAACAAC
TGTCGACGACGGTACTGGAGGGTGATGCATTACAATTATCCGGGCGTGGGAGTTGGTTTGTTGCTCAAGCGGATGAATTA
GCCACGTTGCAACAGCGCGTTACTGCGGGTGAACTGAATATCACCGCGCCTTTACCGGGGGACAGTGAACTGGGTACCCA
CGGTGAGGCATTAGCCTTTGAACAGGCGTGTTTGGCAGAGCAAACTGAATTACTGTCATTGATTAAGCGTGAGCGCGTTG
AAGGTAGCCGCCGGGCAGTACTGCTAAAACCGCAGAATATGATAAGCAACTGGTGGGATGATGTCACTCTTGAGCTGAGT
TTCTGGCTCCCTGCGGGGAGCTTTGCGACCAGTGTAGTGAGGGAAATAATGAACCAGGATCGGGCTGATGATACGGATAT
TATTGAGTAA

Upstream 100 bases:

>100_bases
ACCACCACGGAACAGCTTGGTTTTACCGGGCGTGGTGAAGGTATTGCGTGTGAAGCGGTGGTTTTATTAGTTAACGTAGA
ACAGGGTTAAGGCATAACGA

Downstream 100 bases:

>100_bases
CGATGATGGCATCTCTGCGCCAGGGATCCAGACGCTGGCCAGTGCATTGCGGGAATTTGCTCAGGTGCAAATTGTAGCAC
CCGATCGTAACCGCAGTGGC

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
MDMENLTWLHGKPTASGILKANPEDFVVVEDLGFEPDGEGEHLLVRIRKNGCNTQFVADYLARFAKLHPRLVSYAGLKDR
HAVTEQWFCLHLPGKEAPDLATFELEGCEVLEAVRHKRKLRIGSLKGNAFTLVLRHITDRQDVEQRLQQIAAQGVPNYFG
SQRFGRGGNNLVQARLWANNEIRVKERSKRSFYLSASRSAMFNLISSHRLAQQLSTTVLEGDALQLSGRGSWFVAQADEL
ATLQQRVTAGELNITAPLPGDSELGTHGEALAFEQACLAEQTELLSLIKRERVEGSRRAVLLKPQNMISNWWDDVTLELS
FWLPAGSFATSVVREIMNQDRADDTDIIE

Sequences:

>Translated_349_residues
MDMENLTWLHGKPTASGILKANPEDFVVVEDLGFEPDGEGEHLLVRIRKNGCNTQFVADYLARFAKLHPRLVSYAGLKDR
HAVTEQWFCLHLPGKEAPDLATFELEGCEVLEAVRHKRKLRIGSLKGNAFTLVLRHITDRQDVEQRLQQIAAQGVPNYFG
SQRFGRGGNNLVQARLWANNEIRVKERSKRSFYLSASRSAMFNLISSHRLAQQLSTTVLEGDALQLSGRGSWFVAQADEL
ATLQQRVTAGELNITAPLPGDSELGTHGEALAFEQACLAEQTELLSLIKRERVEGSRRAVLLKPQNMISNWWDDVTLELS
FWLPAGSFATSVVREIMNQDRADDTDIIE
>Mature_349_residues
MDMENLTWLHGKPTASGILKANPEDFVVVEDLGFEPDGEGEHLLVRIRKNGCNTQFVADYLARFAKLHPRLVSYAGLKDR
HAVTEQWFCLHLPGKEAPDLATFELEGCEVLEAVRHKRKLRIGSLKGNAFTLVLRHITDRQDVEQRLQQIAAQGVPNYFG
SQRFGRGGNNLVQARLWANNEIRVKERSKRSFYLSASRSAMFNLISSHRLAQQLSTTVLEGDALQLSGRGSWFVAQADEL
ATLQQRVTAGELNITAPLPGDSELGTHGEALAFEQACLAEQTELLSLIKRERVEGSRRAVLLKPQNMISNWWDDVTLELS
FWLPAGSFATSVVREIMNQDRADDTDIIE

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Escherichia coli, GI1789102, Length=338, Percent_Identity=66.5680473372781, Blast_Score=474, Evalue=1e-135,
Organism=Drosophila melanogaster, GI21355799, Length=132, Percent_Identity=31.8181818181818, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 39149; Mature: 39149

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMENLTWLHGKPTASGILKANPEDFVVVEDLGFEPDGEGEHLLVRIRKNGCNTQFVADY
CCCCCCEEECCCCCCCCCEECCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHH
LARFAKLHPRLVSYAGLKDRHAVTEQWFCLHLPGKEAPDLATFELEGCEVLEAVRHKRKL
HHHHHHHCHHHHHHCCCCHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHE
RIGSLKGNAFTLVLRHITDRQDVEQRLQQIAAQGVPNYFGSQRFGRGGNNLVQARLWANN
EEECCCCCHHEEHHHHHCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEEECCC
EIRVKERSKRSFYLSASRSAMFNLISSHRLAQQLSTTVLEGDALQLSGRGSWFVAQADEL
EEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECHHHH
ATLQQRVTAGELNITAPLPGDSELGTHGEALAFEQACLAEQTELLSLIKRERVEGSRRAV
HHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LLKPQNMISNWWDDVTLELSFWLPAGSFATSVVREIMNQDRADDTDIIE
EECCHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MDMENLTWLHGKPTASGILKANPEDFVVVEDLGFEPDGEGEHLLVRIRKNGCNTQFVADY
CCCCCCEEECCCCCCCCCEECCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHHH
LARFAKLHPRLVSYAGLKDRHAVTEQWFCLHLPGKEAPDLATFELEGCEVLEAVRHKRKL
HHHHHHHCHHHHHHCCCCHHHHHCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHE
RIGSLKGNAFTLVLRHITDRQDVEQRLQQIAAQGVPNYFGSQRFGRGGNNLVQARLWANN
EEECCCCCHHEEHHHHHCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEEEECCC
EIRVKERSKRSFYLSASRSAMFNLISSHRLAQQLSTTVLEGDALQLSGRGSWFVAQADEL
EEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECHHHH
ATLQQRVTAGELNITAPLPGDSELGTHGEALAFEQACLAEQTELLSLIKRERVEGSRRAV
HHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
LLKPQNMISNWWDDVTLELSFWLPAGSFATSVVREIMNQDRADDTDIIE
EECCHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA