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The map label for this gene is hpaG [H]

Identifier: 170024686

GI number: 170024686

Start: 2694105

End: 2694737

Strand: Reverse

Name: hpaG [H]

Synonym: YPK_2461

Alternate gene names: 170024686

Gene position: 2694737-2694105 (Counterclockwise)

Preceding gene: 170024688

Following gene: 170024685

Centisome position: 57.46

GC content: 51.34

Gene sequence:

>633_bases
ATGAAAGGTACCGTATTTGCCGTTGCATTGAACCATCAAAGTCAGATAGAGGCTTGGGATAGCACCTTTCATCAGGCACC
TTATAACCGCCCCCCGAAAACGCCGGTGTGGTTTATCAAACCGCGTAATACCGTGATACGCCATGGTGATGGCATCCCGT
ACCCAGAGGGATTTACCGTGATGAGTGGTGCCACCTTGGCACTGGTTGTTGGTCAGGTTGCCCGTCATGTTGCCTTAAAG
GACGCGGCGTACTATATCGCCGGTTTTGCGGTAGCAAATGAAGTCAGCTTGCCGGAAAGCCATTTTTACCGTCCCGCCAT
TCAAGCAAAATGCCGTGATGGATTTTGTCCTTTGGGCGATCTGGGGCCGTTTATTGATACCTCTGCGCTTGAGATTATTA
CCTTGATTAATGGGCAAGAGGCTGATCGCTGGTCAAGCTCGGGGTTGGTCAGATCAGGCACTCAACTTCTTAGTGCACTG
AGTGAATTCATCACGTTACAACCGGGGGATGTTCTCTTGTTGGGGACGCCACTGCATCGGGTTGAACTCGGCCTTGGAGA
CCAGGTGCAAATACAGGTTGCAGGGCTGCCTGTGCTGGAAAATATCGTGATCCAGCAAGGAAGTAGGCCATGA

Upstream 100 bases:

>100_bases
AAATATAGAATTGGTTAATTATTGACCTGTTGATTCAAATGATTGTTAATATGTTAACTATGTGTTGGGCGGATTCTGTC
TAAGCCAAAAGGAACATTGC

Downstream 100 bases:

>100_bases
AACATGCTCGCATCCAATACCAAGGCAGTACCTTTCATGTCACTGTCGATCCACAAGGCAACATTTGTTTGCCAGATGGT
CGCAATGTTAGCAGTGAGCA

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase subunit HpaG1

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKGTVFAVALNHQSQIEAWDSTFHQAPYNRPPKTPVWFIKPRNTVIRHGDGIPYPEGFTVMSGATLALVVGQVARHVALK
DAAYYIAGFAVANEVSLPESHFYRPAIQAKCRDGFCPLGDLGPFIDTSALEIITLINGQEADRWSSSGLVRSGTQLLSAL
SEFITLQPGDVLLLGTPLHRVELGLGDQVQIQVAGLPVLENIVIQQGSRP

Sequences:

>Translated_210_residues
MKGTVFAVALNHQSQIEAWDSTFHQAPYNRPPKTPVWFIKPRNTVIRHGDGIPYPEGFTVMSGATLALVVGQVARHVALK
DAAYYIAGFAVANEVSLPESHFYRPAIQAKCRDGFCPLGDLGPFIDTSALEIITLINGQEADRWSSSGLVRSGTQLLSAL
SEFITLQPGDVLLLGTPLHRVELGLGDQVQIQVAGLPVLENIVIQQGSRP
>Mature_210_residues
MKGTVFAVALNHQSQIEAWDSTFHQAPYNRPPKTPVWFIKPRNTVIRHGDGIPYPEGFTVMSGATLALVVGQVARHVALK
DAAYYIAGFAVANEVSLPESHFYRPAIQAKCRDGFCPLGDLGPFIDTSALEIITLINGQEADRWSSSGLVRSGTQLLSAL
SEFITLQPGDVLLLGTPLHRVELGLGDQVQIQVAGLPVLENIVIQQGSRP

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=197, Percent_Identity=32.48730964467, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI40786394, Length=197, Percent_Identity=32.48730964467, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1787428, Length=174, Percent_Identity=28.1609195402299, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557057, Length=172, Percent_Identity=29.6511627906977, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28572127, Length=220, Percent_Identity=30, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571789, Length=205, Percent_Identity=26.8292682926829, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 22753; Mature: 22753

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGTVFAVALNHQSQIEAWDSTFHQAPYNRPPKTPVWFIKPRNTVIRHGDGIPYPEGFTV
CCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCEEE
MSGATLALVVGQVARHVALKDAAYYIAGFAVANEVSLPESHFYRPAIQAKCRDGFCPLGD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHCCCCCCCCCC
LGPFIDTSALEIITLINGQEADRWSSSGLVRSGTQLLSALSEFITLQPGDVLLLGTPLHR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHE
VELGLGDQVQIQVAGLPVLENIVIQQGSRP
EEECCCCEEEEEEECCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKGTVFAVALNHQSQIEAWDSTFHQAPYNRPPKTPVWFIKPRNTVIRHGDGIPYPEGFTV
CCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCEEE
MSGATLALVVGQVARHVALKDAAYYIAGFAVANEVSLPESHFYRPAIQAKCRDGFCPLGD
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHCCCCCCCCCC
LGPFIDTSALEIITLINGQEADRWSSSGLVRSGTQLLSALSEFITLQPGDVLLLGTPLHR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCHHE
VELGLGDQVQIQVAGLPVLENIVIQQGSRP
EEECCCCEEEEEEECCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA