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The map label for this gene is hpaG [H]

Identifier: 170024685

GI number: 170024685

Start: 2693344

End: 2694108

Strand: Reverse

Name: hpaG [H]

Synonym: YPK_2460

Alternate gene names: 170024685

Gene position: 2694108-2693344 (Counterclockwise)

Preceding gene: 170024686

Following gene: 170024684

Centisome position: 57.45

GC content: 48.1

Gene sequence:

>765_bases
ATGAAACATGCTCGCATCCAATACCAAGGCAGTACCTTTCATGTCACTGTCGATCCACAAGGCAACATTTGTTTGCCAGA
TGGTCGCAATGTTAGCAGTGAGCACGTTATATGGTTGCCTCCTGCGACAGGAACGTTATTTGCACTCGGATTGAATTATG
CTGATCACGCGGCAGAGTTGGATTTCAGCCCCCCGAAAGAGCCACTGATTTTTATTAAAGCGCCCCATAGCTTCACCGGA
CATCGTCAGGTTTCCGTCCGCCCCGATAACATTGAATATATGCATTATGAAGCCGAGCTCGTGGTCGTGGTTGGCAAACC
AGCGCGTTACCTCAACCCAGCTCACGCCATGGATTATGTTGCCGGTTATACCTTGTGTAATGACTATGCCGTTCGTGACT
ATTTAGAGAACTACTATCGTCCCAATCTACGGGTAAAAAGCCGCGATACCTTGACGCCTATCGGCCCATGGATAGTCGAT
AAAGCGGATATTCCCAATCCTCATAATTTGGTTATTCGCACTTGGGTCAACGGTGAATTATGCCAGCGAGGTAACACCGC
CGATCTGATTTTCGATATTCCTTTTCTGATGGCGTATCTGAGTGAGTTTATAACCTTACAACCCGGTGACATGATCGCCA
CGGGAACGCCCAAAGGCCTATCGAATGTGGTTCCGGGGGATGAAGTCGTGGTTGAGGTAGATGGTATTGGTTGCCTGCTT
AACCGTGTTATCAGCCAAACAGACTATGAGGATGGATTGCTATGA

Upstream 100 bases:

>100_bases
CACTGCATCGGGTTGAACTCGGCCTTGGAGACCAGGTGCAAATACAGGTTGCAGGGCTGCCTGTGCTGGAAAATATCGTG
ATCCAGCAAGGAAGTAGGCC

Downstream 100 bases:

>100_bases
AGATTGTTAACCATTGGATTGATGGGAAAAATATTACCAGCAATGACTATTTCACAACAATCAATCCGGCCACTGGCGAG
GTGCTGGCTGACGTGGCAAG

Product: 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase subunit HpaG2

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAELDFSPPKEPLIFIKAPHSFTG
HRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYVAGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVD
KADIPNPHNLVIRTWVNGELCQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDGIGCLL
NRVISQTDYEDGLL

Sequences:

>Translated_254_residues
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAELDFSPPKEPLIFIKAPHSFTG
HRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYVAGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVD
KADIPNPHNLVIRTWVNGELCQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDGIGCLL
NRVISQTDYEDGLL
>Mature_254_residues
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAELDFSPPKEPLIFIKAPHSFTG
HRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYVAGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVD
KADIPNPHNLVIRTWVNGELCQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDGIGCLL
NRVISQTDYEDGLL

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=243, Percent_Identity=35.3909465020576, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI40786394, Length=221, Percent_Identity=37.10407239819, Blast_Score=147, Evalue=6e-36,
Organism=Homo sapiens, GI66348062, Length=194, Percent_Identity=38.659793814433, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI13654274, Length=201, Percent_Identity=37.8109452736318, Blast_Score=135, Evalue=3e-32,
Organism=Homo sapiens, GI215422413, Length=194, Percent_Identity=38.659793814433, Blast_Score=135, Evalue=4e-32,
Organism=Escherichia coli, GI1787428, Length=199, Percent_Identity=35.1758793969849, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557057, Length=187, Percent_Identity=38.5026737967914, Blast_Score=137, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6324161, Length=226, Percent_Identity=27.8761061946903, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572127, Length=223, Percent_Identity=40.3587443946188, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24663695, Length=216, Percent_Identity=34.7222222222222, Blast_Score=127, Evalue=6e-30,
Organism=Drosophila melanogaster, GI28571789, Length=188, Percent_Identity=38.8297872340425, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 28336; Mature: 28336

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAEL
CCCCEEEECCCEEEEEECCCCCEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEC
DFSPPKEPLIFIKAPHSFTGHRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYV
CCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEEEEEEEEECCCHHHCCHHHHHHHH
AGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVDKADIPNPHNLVIRTWVNGEL
HHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEECCCCCCCCCEEEEEECCCHH
CQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDGIGCLL
HHCCCCCEEEEEHHHHHHHHHHHEEECCCCEEECCCCCCCCCCCCCCEEEEEECCHHHHH
NRVISQTDYEDGLL
HHHHHHCCCCCCCC
>Mature Secondary Structure
MKHARIQYQGSTFHVTVDPQGNICLPDGRNVSSEHVIWLPPATGTLFALGLNYADHAAEL
CCCCEEEECCCEEEEEECCCCCEECCCCCCCCCCEEEEECCCCCEEEEEECCCCCCCEEC
DFSPPKEPLIFIKAPHSFTGHRQVSVRPDNIEYMHYEAELVVVVGKPARYLNPAHAMDYV
CCCCCCCCEEEEECCCCCCCCEEEEECCCCEEEEEEEEEEEEEECCCHHHCCHHHHHHHH
AGYTLCNDYAVRDYLENYYRPNLRVKSRDTLTPIGPWIVDKADIPNPHNLVIRTWVNGEL
HHHHHHCCHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEECCCCCCCCCEEEEEECCCHH
CQRGNTADLIFDIPFLMAYLSEFITLQPGDMIATGTPKGLSNVVPGDEVVVEVDGIGCLL
HHCCCCCEEEEEHHHHHHHHHHHEEECCCCEEECCCCCCCCCCCCCCEEEEEECCHHHHH
NRVISQTDYEDGLL
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA