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The map label for this gene is cusS [H]

Identifier: 170024424

GI number: 170024424

Start: 2430680

End: 2432065

Strand: Direct

Name: cusS [H]

Synonym: YPK_2193

Alternate gene names: 170024424

Gene position: 2430680-2432065 (Clockwise)

Preceding gene: 170024423

Following gene: 170024425

Centisome position: 51.83

GC content: 51.59

Gene sequence:

>1386_bases
ATGATTAAGCGTTCTATCTCTGGGCGGCTGGCGCTAATGTTTGCCTTGGCCTCCATACTGATAGTTTCGGCTCTCGGTAT
TGTACTGCGAAGCTCCTTGCATAATTCATTGCAAAAGCAGATGCATAACGAATTGCAATTCAGGGAGTCGCTGATGGCAC
CGTGGATCCTCTCCCGTACCACGCTGGACGGCTGGCAAACGTTGGCGACCAAATTCACCGATTTGGCCGCTGCTGAGGGC
GGCCGCGTACAATACTGGGTCCTCAGTGATGACCCCCAGCTTCAGGTAGGCGGCCCGGCACCCCGTGGTGTAACCTGGGC
TTCGTTAAGCAAAGGATTCAGCAAAGTACCCGGTGCGACAGACGAGTCATGCTCCCTATTTCTACTGATGTCGGAAATTC
CTGCCCAAGGTGAGCGCCCCGCTCTGCGCTATGTGGTCGCTATCGATTCCACGCCGTATATGGGTACTCTGAATGAATTT
ACCCGCACGCTGATCGTCATTACTGCGATTGGCGTCTTATTCGTGGCACTGCTGGGGTTCGTGATTGCCAGAATAGGTAT
GCGCCCTGTTAATAAACTCAGCGAACAGGCCCAGCACCTTGCGCCAGGAAATCACGGGCAGCGTCTGGATACCTTAACTC
TGCCGGATGAACTGCAAAAACTGGCCGTCGCATTCAACGGCGTGCTCGAACGGCAGGAAATCGCCTGGCGGCAACTTGAA
AGTTTTAATGCTGATGTCGCACATGAACTGAGAACCCCGTTGACCAATCTGATGGGGCAAACGCAATTAGGTCTATCTCG
TAAACGGGAAACGCATGAGCTCGAAGATTTACTGGAGTCCAATCTGGAAGAACTCGAGAGAATGACCTCTATTGTTAACG
ATATGCTGTTCCTCTCCCATGCTCAAGCTGGGGAGCACGCAACCCAGTTAACCGACGTGTCTCTTCGGGAAGAGACCTAT
AAGACCACGGAGTATGTAGAACCTTCATTTGCCGAAAAAAATTTGTCTATTCATGTTGAGGGAGATATCACTGCACGCAT
TGATCGGCGGCTTTTCCACCGCTCACTGGCGAATCTGCTAGAAAACAGCGCCCGCCATGCTGTTACTGGTACTACCGTGA
AAGTGACGATCTCCAGAGAAAATAATCTGGCATCCATCGCGGTAGCCAACATTGGAGCTCCCATTGCGGCGGAACATCTA
AACCGTTTGTTTGAGCGTTTTTATCGTGTTGATTCTTCCCGTGCACGCAGTGACACACACCATGGACTCGGGCTCTCCAT
CGTCAGGGCGGTGGCGATAATGCATCGTGGAGATGTGTTTGCCAGTAGCCAGAATGGGATTAATACCTTTGGTCTGACCC
TTGCAATAACTGACCCTTATATATAG

Upstream 100 bases:

>100_bases
TAGATACGCCCTACCCGGTCAAACTGCTGCATACAGTGCGAGGCATGGGGTATGTCCTTGAAACTCGCCCCGATCGCCAG
ACTAAATAGAGAGAGTGATC

Downstream 100 bases:

>100_bases
ACAGTGAGTCCGTTGAAAAAGAAATGAGTCGTTATGTGAAGTCTGGCCTGGCTGAGCGAGGGTAACGTCTATGACAGTGT
ATTTTTGCATACAGGTTTAA

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRTTLDGWQTLATKFTDLAAAEG
GRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEF
TRTLIVITAIGVLFVALLGFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSHAQAGEHATQLTDVSLREETY
KTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLANLLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHL
NRLFERFYRVDSSRARSDTHHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI

Sequences:

>Translated_461_residues
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRTTLDGWQTLATKFTDLAAAEG
GRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEF
TRTLIVITAIGVLFVALLGFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSHAQAGEHATQLTDVSLREETY
KTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLANLLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHL
NRLFERFYRVDSSRARSDTHHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI
>Mature_461_residues
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRTTLDGWQTLATKFTDLAAAEG
GRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGATDESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEF
TRTLIVITAIGVLFVALLGFVIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSHAQAGEHATQLTDVSLREETY
KTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLANLLENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHL
NRLFERFYRVDSSRARSDTHHGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=322, Percent_Identity=35.0931677018634, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI1788279, Length=316, Percent_Identity=30.379746835443, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788393, Length=259, Percent_Identity=31.2741312741313, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786600, Length=250, Percent_Identity=28.8, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787894, Length=305, Percent_Identity=27.8688524590164, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790346, Length=262, Percent_Identity=27.8625954198473, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1789403, Length=289, Percent_Identity=26.643598615917, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI145693157, Length=212, Percent_Identity=27.3584905660377, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790551, Length=313, Percent_Identity=28.4345047923323, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786912, Length=254, Percent_Identity=26.7716535433071, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790861, Length=233, Percent_Identity=25.3218884120172, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50981; Mature: 50981

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
TLDGWQTLATKFTDLAAAEGGRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGAT
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCHHHHHCCHHHCCCCC
DESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEFTRTLIVITAIGVLFVALLGF
CCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
HHHHHCCCHHHHHHHHHHHCCCCCCCCEECEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSH
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAGEHATQLTDVSLREETYKTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLANLL
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
ENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHLNRLFERFYRVDSSRARSDTH
HCCCCCEEECCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCCCC
HGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI
CCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEECCCCC
>Mature Secondary Structure
MIKRSISGRLALMFALASILIVSALGIVLRSSLHNSLQKQMHNELQFRESLMAPWILSRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHC
TLDGWQTLATKFTDLAAAEGGRVQYWVLSDDPQLQVGGPAPRGVTWASLSKGFSKVPGAT
CHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCCCCHHHHHCCHHHCCCCC
DESCSLFLLMSEIPAQGERPALRYVVAIDSTPYMGTLNEFTRTLIVITAIGVLFVALLGF
CCCCEEEEEEECCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VIARIGMRPVNKLSEQAQHLAPGNHGQRLDTLTLPDELQKLAVAFNGVLERQEIAWRQLE
HHHHHCCCHHHHHHHHHHHCCCCCCCCEECEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
SFNADVAHELRTPLTNLMGQTQLGLSRKRETHELEDLLESNLEELERMTSIVNDMLFLSH
HCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQAGEHATQLTDVSLREETYKTTEYVEPSFAEKNLSIHVEGDITARIDRRLFHRSLANLL
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
ENSARHAVTGTTVKVTISRENNLASIAVANIGAPIAAEHLNRLFERFYRVDSSRARSDTH
HCCCCCEEECCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHCCCCC
HGLGLSIVRAVAIMHRGDVFASSQNGINTFGLTLAITDPYI
CCCHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]