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The map label for this gene is mdtL [H]

Identifier: 170024340

GI number: 170024340

Start: 2335324

End: 2336517

Strand: Direct

Name: mdtL [H]

Synonym: YPK_2106

Alternate gene names: 170024340

Gene position: 2335324-2336517 (Clockwise)

Preceding gene: 170024339

Following gene: 170024342

Centisome position: 49.8

GC content: 50.25

Gene sequence:

>1194_bases
ATGCAAAAATTCCTTTTCCTGCTATTAAGCTTGATTTTACTTGGCCCTTTGGGGATCGATCTCTATCTACCGACCATTCC
AGCCATTGCAAAAGGGCTGAATAGTAGTGAGTCTCTGATTCAATCAACTATTGCGCTGTTTATTCTCGTGCTGGGTATCG
GGCAACTGATTGCTGGGCCATTGGTTGATAAATATGGCCGTAAGCCAATCGCTATTATCGGTATTGTTCTTTACATGCTG
GGTGCCGCGATGGCCGCTCTGGCCGTGGATCCAGTCATGTTCGTCAGCTCCCGCTTGTTACAAGGTGTCTCGGTATGCTG
CACAGCCGTGGTGGCCTTCAGTGGCGTCCGTGACCGCCTTAGCGGCAATGAAGCCGCACGTGCTTTTGGCTTCCTTAATG
GCACGTTGAATATTGTCCCCGCATTGGCCCCGCTATTAGGTGGTTTACTGGCCGAAGCCTTTGGCTGGCGGGCACCGTTC
TGGTTCCTGGCATTATACGGATTACTGGTTTTAGTCTTAATCATTATGTTCTTACCCGAAACCCGCCCGGCGAATACTCA
ACCAATAAATGGCCTGCCAATAAAAAACTACCTGCGTATTTTGCGTGATGGCCGCTTTTTGGTATTTGCTCTGGTCAATG
CAGGGGCCATGGGCATGGCATTAACCTATGTATCATTGGCACCGAATGTATTGATGGTTGATGCAGGCCTCAGCCCGTTA
CAATTTTCGCTGGCATTTGGGGCTAATGGCTTCTGGATTATGGCAGTCAGTTTTTTTGCTAACCGGATTATTCAAAAAGT
GGGCCGCCCTACCTGCCTGATGATCGGTAGCTTATTGATGGCCTTAGGCTGTTTTGGCTTGCTATTTGGTCTGACACAAC
TGTCCGTTGATGTGCAGCATCACTGGCTGGTTTATATGTTACCGGTTGCCAGCGCCTGTGCCGGTTTAGCCTTTATGATG
GGGCCTGCCACCAGTTATGCTCTAGAACCCTATGCGAATGAAGCGGGTGTTGCCTCAGCGTTAGTGGGATTTGTTCAAAT
GGCTGGGGGCGCTGCATTAGGCCTGTTGGCGATGGCACTGCCGATTGAACCTAAGTTGTCACTGGCTATCGTGATGCTGG
CTGGCTGTTTACTGGCGTGGCAAGCGCGCCGAGCCAGTAAACATGTCCGCGGCAAGTTGGAAGAAATACACTAA

Upstream 100 bases:

>100_bases
GACAGTGTCACAAAATCACGCTTAGCACTTATGGGTTTCCTATTTACGGGTAAAGCCCTTATTCTTGTCGCCTTTGTAAT
TTGGATGCGATTTCTTTTTC

Downstream 100 bases:

>100_bases
CAACGGCAAATAATCGCGTTACACGATATTAACGGATGGGGTGAATATCGTGTAACGGGGCAATATTCAGTGGCTGAAAA
CCTGTGCGGTAACGCGCTAC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGPLVDKYGRKPIAIIGIVLYML
GAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRLSGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPF
WFLALYGLLVLVLIIMFLPETRPANTQPINGLPIKNYLRILRDGRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQHHWLVYMLPVASACAGLAFMM
GPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMALPIEPKLSLAIVMLAGCLLAWQARRASKHVRGKLEEIH

Sequences:

>Translated_397_residues
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGPLVDKYGRKPIAIIGIVLYML
GAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRLSGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPF
WFLALYGLLVLVLIIMFLPETRPANTQPINGLPIKNYLRILRDGRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQHHWLVYMLPVASACAGLAFMM
GPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMALPIEPKLSLAIVMLAGCLLAWQARRASKHVRGKLEEIH
>Mature_397_residues
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGPLVDKYGRKPIAIIGIVLYML
GAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRLSGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPF
WFLALYGLLVLVLIIMFLPETRPANTQPINGLPIKNYLRILRDGRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQHHWLVYMLPVASACAGLAFMM
GPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMALPIEPKLSLAIVMLAGCLLAWQARRASKHVRGKLEEIH

Specific function: Confers resistance to chloramphenicol [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=404, Percent_Identity=32.4257425742574, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788509, Length=358, Percent_Identity=27.6536312849162, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI48994889, Length=216, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082312, Length=355, Percent_Identity=27.887323943662, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790794, Length=252, Percent_Identity=27.3809523809524, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1787065, Length=233, Percent_Identity=21.4592274678112, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081738, Length=192, Percent_Identity=26.5625, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324264, Length=166, Percent_Identity=26.5060240963855, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322071, Length=164, Percent_Identity=30.4878048780488, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319517, Length=182, Percent_Identity=25.2747252747253, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42118; Mature: 42118

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGP
CHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVDKYGRKPIAIIGIVLYMLGAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPFWFLALYGLLVLVLIIMFLPE
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
TRPANTQPINGLPIKNYLRILRDGRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHHCCCEEEEECCCCCC
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQH
EEEHEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWLVYMLPVASACAGLAFMMGPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMAL
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHC
PIEPKLSLAIVMLAGCLLAWQARRASKHVRGKLEEIH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKFLFLLLSLILLGPLGIDLYLPTIPAIAKGLNSSESLIQSTIALFILVLGIGQLIAGP
CHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LVDKYGRKPIAIIGIVLYMLGAAMAALAVDPVMFVSSRLLQGVSVCCTAVVAFSGVRDRL
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGNEAARAFGFLNGTLNIVPALAPLLGGLLAEAFGWRAPFWFLALYGLLVLVLIIMFLPE
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
TRPANTQPINGLPIKNYLRILRDGRFLVFALVNAGAMGMALTYVSLAPNVLMVDAGLSPL
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHHCCCEEEEECCCCCC
QFSLAFGANGFWIMAVSFFANRIIQKVGRPTCLMIGSLLMALGCFGLLFGLTQLSVDVQH
EEEHEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HWLVYMLPVASACAGLAFMMGPATSYALEPYANEAGVASALVGFVQMAGGAALGLLAMAL
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHC
PIEPKLSLAIVMLAGCLLAWQARRASKHVRGKLEEIH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA