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The map label for this gene is xthA [H]

Identifier: 170024319

GI number: 170024319

Start: 2314548

End: 2315354

Strand: Direct

Name: xthA [H]

Synonym: YPK_2085

Alternate gene names: 170024319

Gene position: 2314548-2315354 (Clockwise)

Preceding gene: 170024315

Following gene: 170024320

Centisome position: 49.36

GC content: 47.58

Gene sequence:

>807_bases
ATGAAGTTCGTTTCTTTTAATATCAATGGCCTACGTGCTCGTCCTCATCAATTAGCCGCAATAATTGAGCAGCATCAACC
GGATGTGATCGGCCTACAAGAAACTAAGGTACATGATGATATGTTTCCTCTTGAAGACGTCAGCCAGCATGGTTACCACG
TTTTTTACCATGGGCAAAAAGGTCATTACGGTGTTGCACTATTGACGAAAGAACAGCCACTGGCTGTGCGCCGAGGTTTC
CCAAGCGATGAAGAAGATGCACAGCGCCGTATTATCATGGCCGACTTAGCCACCCCGCAAGGCACCTTAACGGTAATCAA
TGGTTATTTTCCTCAGGGAGAAAGTCGTGACCATCCAATAAAGTTCCCCGCTAAAGCGCGCTTTTATGCTGACCTGCAAC
GCTATCTTGAGGAGCAACTCTCCCCTGAGGCCCAGGTACTTATCATGGGGGACTTGAATATCAGTCCAACCGATTTAGAT
ATCGGTATTGGTGAAGATAACCGTAAACGCTGGCTGCGCACAGGCAAATGCTCGTTCTTACCAGAAGAACGAGAGTGGCT
GGAACGTCTGCAAAACTGGGGGTTGGTTGATACTTTCCGCGCGGCTAATCCTGATTGTAATGACCATTTTTCATGGTTTG
ATTACCGTTCCCGTGGTTTTGATGAAAACAGAGGCCTTCGCATCGATTTATTGCTGGCCAGTCAACCACTGGCGGCTCGT
TGTATTGCTACAGGTATTGATTATGCAATCCGTGGGATGGAAAAACCATCAGACCACGCGCCGGTTTGGTCCGAATTCTC
ACTTTAA

Upstream 100 bases:

>100_bases
GAAATACATCAGATTATTTTATACTCAGCCAGAACATTACATTCTTCTTCTTATTCTGCTAATATCCACTCACATTTTTT
AGCCAAAAACGGTGCTGACC

Downstream 100 bases:

>100_bases
TGTCTGCGCCCTGTTTAAATACAGGGCCACCTCTCAAGCGTATTAAGCACATTTATCCGTCATTAGGTAAATTTATCCAT
AATCAGGTAAACACGAAATG

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKFVSFNINGLRARPHQLAAIIEQHQPDVIGLQETKVHDDMFPLEDVSQHGYHVFYHGQKGHYGVALLTKEQPLAVRRGF
PSDEEDAQRRIIMADLATPQGTLTVINGYFPQGESRDHPIKFPAKARFYADLQRYLEEQLSPEAQVLIMGDLNISPTDLD
IGIGEDNRKRWLRTGKCSFLPEEREWLERLQNWGLVDTFRAANPDCNDHFSWFDYRSRGFDENRGLRIDLLLASQPLAAR
CIATGIDYAIRGMEKPSDHAPVWSEFSL

Sequences:

>Translated_268_residues
MKFVSFNINGLRARPHQLAAIIEQHQPDVIGLQETKVHDDMFPLEDVSQHGYHVFYHGQKGHYGVALLTKEQPLAVRRGF
PSDEEDAQRRIIMADLATPQGTLTVINGYFPQGESRDHPIKFPAKARFYADLQRYLEEQLSPEAQVLIMGDLNISPTDLD
IGIGEDNRKRWLRTGKCSFLPEEREWLERLQNWGLVDTFRAANPDCNDHFSWFDYRSRGFDENRGLRIDLLLASQPLAAR
CIATGIDYAIRGMEKPSDHAPVWSEFSL
>Mature_268_residues
MKFVSFNINGLRARPHQLAAIIEQHQPDVIGLQETKVHDDMFPLEDVSQHGYHVFYHGQKGHYGVALLTKEQPLAVRRGF
PSDEEDAQRRIIMADLATPQGTLTVINGYFPQGESRDHPIKFPAKARFYADLQRYLEEQLSPEAQVLIMGDLNISPTDLD
IGIGEDNRKRWLRTGKCSFLPEEREWLERLQNWGLVDTFRAANPDCNDHFSWFDYRSRGFDENRGLRIDLLLASQPLAAR
CIATGIDYAIRGMEKPSDHAPVWSEFSL

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=268, Percent_Identity=26.4925373134328, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI18375503, Length=268, Percent_Identity=26.4925373134328, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI18375501, Length=268, Percent_Identity=26.4925373134328, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=80.0751879699248, Blast_Score=457, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI71989536, Length=266, Percent_Identity=26.6917293233083, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI221330655, Length=271, Percent_Identity=30.2583025830258, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17136678, Length=271, Percent_Identity=30.2583025830258, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30720; Mature: 30720

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVSFNINGLRARPHQLAAIIEQHQPDVIGLQETKVHDDMFPLEDVSQHGYHVFYHGQK
CEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHCCCEEEEEECCC
GHYGVALLTKEQPLAVRRGFPSDEEDAQRRIIMADLATPQGTLTVINGYFPQGESRDHPI
CCEEEEEEECCCCHHHHCCCCCCHHHHHHEEEEEECCCCCCEEEEEEEECCCCCCCCCCE
KFPAKARFYADLQRYLEEQLSPEAQVLIMGDLNISPTDLDIGIGEDNRKRWLRTGKCSFL
ECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCCHHHHHHCCCCCCC
PEEREWLERLQNWGLVDTFRAANPDCNDHFSWFDYRSRGFDENRGLRIDLLLASQPLAAR
CHHHHHHHHHHCCCCEEEHHCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHHH
CIATGIDYAIRGMEKPSDHAPVWSEFSL
HHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKFVSFNINGLRARPHQLAAIIEQHQPDVIGLQETKVHDDMFPLEDVSQHGYHVFYHGQK
CEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHHHCCCEEEEEECCC
GHYGVALLTKEQPLAVRRGFPSDEEDAQRRIIMADLATPQGTLTVINGYFPQGESRDHPI
CCEEEEEEECCCCHHHHCCCCCCHHHHHHEEEEEECCCCCCEEEEEEEECCCCCCCCCCE
KFPAKARFYADLQRYLEEQLSPEAQVLIMGDLNISPTDLDIGIGEDNRKRWLRTGKCSFL
ECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCCHHHHHHCCCCCCC
PEEREWLERLQNWGLVDTFRAANPDCNDHFSWFDYRSRGFDENRGLRIDLLLASQPLAAR
CHHHHHHHHHHCCCCEEEHHCCCCCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCHHHH
CIATGIDYAIRGMEKPSDHAPVWSEFSL
HHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]