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The map label for this gene is hda [H]

Identifier: 170023596

GI number: 170023596

Start: 1495395

End: 1496102

Strand: Direct

Name: hda [H]

Synonym: YPK_1354

Alternate gene names: 170023596

Gene position: 1495395-1496102 (Clockwise)

Preceding gene: 170023595

Following gene: 170023599

Centisome position: 31.89

GC content: 48.02

Gene sequence:

>708_bases
GTGCTTCTGAATACGCCGGCACAGCTTTCACTGCCACTCTATCTTCCCGATGATGAAACTTTTGCCAGTTTTTATCCGGG
GGAGAACCCATCCCTGTTAGCAGCGATCCAATCCGCCGTTCATCAGCCTCATGGCAGTTATATCTATTTCTGGTCGCGAG
AAGGCGGTGGACGTAGTCATTTGCTACATGCCGCTTGTGCTGAGTTATCGCAGCAAGGTGAGGCGGTGGGGTATGTTCCG
CTGGATAAACGCGCTTACTTCATACCTGAAGTATTAGAGGGGATGGAGCAACTGGCGCTGGTCTGCATTGATAATATTGA
GTGTATTGCAGGTGATGAACAGTGGGAAATGGCGATGTTTAATCTCTATAACCGCATTGTGGAAACTGGCCGTACCCGTT
TATTGATCACGGGTGATCGCCCGCCGCGTCAACTCAACCTTGGTTTACCGGATCTGGCCTCGCGCCTGGATTGGGGGCAG
ATTTATAAATTGCAGCCGTTGTCAGATGATGAAAAATTGCAGGCTTTGCAGTTACGCGCCAAATTACGTGGTTTTGAATT
GCCAGAAGATGTTGGCCGCTTTTTACTCAAGCGTTTAGACCGAGAAATGCGCACTTTGTTTATGACGTTGGATCAACTGG
ATCGGGCCTCAATTACGGCACAACGTAAGCTTACTATCCCATTTGTTAAAGAAATTCTTAGTCTTTAA

Upstream 100 bases:

>100_bases
TTGCCAGTTCAGCCCATTTTTCACCAAATTCAGGTTGTTATGTTGTTATAAATTTTTGTGGTAAACTCGTCACGTTTTCC
CGCCCGTTTTGTTGGTTGAG

Downstream 100 bases:

>100_bases
CTATGCCCTTCGTCATGGCGGCTACTGCCCTGTTGATACTCGTCATACTTCAAGTTGTGCGTGCGTTAGCCACGCTCGTT
ACTCGGCCCATCCATGGGAC

Product: DNA replication initiation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MLLNTPAQLSLPLYLPDDETFASFYPGENPSLLAAIQSAVHQPHGSYIYFWSREGGGRSHLLHAACAELSQQGEAVGYVP
LDKRAYFIPEVLEGMEQLALVCIDNIECIAGDEQWEMAMFNLYNRIVETGRTRLLITGDRPPRQLNLGLPDLASRLDWGQ
IYKLQPLSDDEKLQALQLRAKLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILSL

Sequences:

>Translated_235_residues
MLLNTPAQLSLPLYLPDDETFASFYPGENPSLLAAIQSAVHQPHGSYIYFWSREGGGRSHLLHAACAELSQQGEAVGYVP
LDKRAYFIPEVLEGMEQLALVCIDNIECIAGDEQWEMAMFNLYNRIVETGRTRLLITGDRPPRQLNLGLPDLASRLDWGQ
IYKLQPLSDDEKLQALQLRAKLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILSL
>Mature_235_residues
MLLNTPAQLSLPLYLPDDETFASFYPGENPSLLAAIQSAVHQPHGSYIYFWSREGGGRSHLLHAACAELSQQGEAVGYVP
LDKRAYFIPEVLEGMEQLALVCIDNIECIAGDEQWEMAMFNLYNRIVETGRTRLLITGDRPPRQLNLGLPDLASRLDWGQ
IYKLQPLSDDEKLQALQLRAKLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILSL

Specific function: Mediates the interactions of dnaA with the beta subunit sliding clamp, promoting hydrolysis of dnaA-bound ATP. It is essential for the control of initiation of DNA replication by inhibiting reinitiation of replication [H]

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family. HdA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510964, Length=233, Percent_Identity=85.8369098712446, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI2367267, Length=244, Percent_Identity=25.8196721311475, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020591
- InterPro:   IPR017788
- InterPro:   IPR013317
- InterPro:   IPR022864 [H]

Pfam domain/function: PF00308 Bac_DnaA [H]

EC number: NA

Molecular weight: Translated: 26703; Mature: 26703

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNTPAQLSLPLYLPDDETFASFYPGENPSLLAAIQSAVHQPHGSYIYFWSREGGGRSH
CCCCCCCCEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
LLHAACAELSQQGEAVGYVPLDKRAYFIPEVLEGMEQLALVCIDNIECIAGDEQWEMAMF
HHHHHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
NLYNRIVETGRTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPLSDDEKLQALQLRA
HHHHHHHHCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILSL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHCC
>Mature Secondary Structure
MLLNTPAQLSLPLYLPDDETFASFYPGENPSLLAAIQSAVHQPHGSYIYFWSREGGGRSH
CCCCCCCCEEEEEECCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
LLHAACAELSQQGEAVGYVPLDKRAYFIPEVLEGMEQLALVCIDNIECIAGDEQWEMAMF
HHHHHHHHHHHCCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHH
NLYNRIVETGRTRLLITGDRPPRQLNLGLPDLASRLDWGQIYKLQPLSDDEKLQALQLRA
HHHHHHHHCCCCEEEEECCCCCCEECCCCHHHHHHCCCCCEEEECCCCCHHHHHHHHHHH
KLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITAQRKLTIPFVKEILSL
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA