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The map label for this gene is potD-A [H]

Identifier: 170023578

GI number: 170023578

Start: 1472988

End: 1474016

Strand: Direct

Name: potD-A [H]

Synonym: YPK_1336

Alternate gene names: 170023578

Gene position: 1472988-1474016 (Clockwise)

Preceding gene: 170023577

Following gene: 170023579

Centisome position: 31.41

GC content: 48.2

Gene sequence:

>1029_bases
ATGTTTAAAATAATAACACTGACTACGCTGCTCGCCGGATGGGTATTTCAAACGCACGCTGAAACCCTGACGGTGGTGTC
ATTCGGTGGTTTAAACAAAGACGCACAGGTTAAGGCGTTCTATAAACCTTTCATGGCCGCCGGGAAAGGAACAATTGAAG
CCGGGGAGTATAACGGTGAAATGGCCCGTATCCGTGCGATGGTGGAAACGGGTCAAATAGGGTGGGATATCGTCGAAGTT
GAAGGCCCAGAACTGCTACGGGGATGCAATGAAGGGCTATTTGAAACCCTGGATTGGAGCAAGTTAGGCGATGAACGTCA
GTTCATTAAAGGCACGGTTAGCCAATGTGGTTCGGGTCTTTTTTTGTGGTCAATGCTATTAATCTACGACGCCAGCAAAT
TTAAAACCCCGGTGACCAGTTGGGCTGACTTCTGGAATGTCACCACTTTTCCCGGCAAACGTTCCCTGCGCAAAACTGCC
AAATTTACCATGGAAATTGCGTTGCTGGCGGATGGCGTGAAACGTGAGGATGTGTATCGTGTGCTGGCGACGCCGGAAGG
TGTTGAACGTGTTTTCAACAAACTGGATCAACTTAAACCGAATATCCAGTGGTGGGAATCTGGGGCGCAACCGCTGCAAT
GGCTGGTGTCCGGTGATGTTGTGATGGGGTCAGTCTATAATGGCCGTGTGGGGTCCGCGCTAAAAGAAGGCCATGATTTT
AGAATGGTTTGGACTGACAGTATCTACGATATGGACAGTTGGACTATCGTCAAAGGCTCCAAAAAAAAAGTATTGGCAGA
ACAATTTATCGCCTTTGCCAACTTAGCGGAAAATCAGAAAGTCTTTGCAGAAAATATCGCTTATGGCCCGACCAATATCA
ACGCCACCTTGATGATTGATCCCGAAATCGCCGCTAATTTACCCACTGCGCCAGCCAACCTGGCTGAGGCTTTTCCCATG
AACAATCAATTCTGGGTCGAGCATAGCGAAGATCTTGAGCAACGCTTCAACTCTTGGGCAGCTAAATAA

Upstream 100 bases:

>100_bases
CAATTTAAACAGGCGATGAATATTCTTACCCGCCTACTGGCGAGCTAAAGGTTTTATCTCACTGATCTCGAAACGTTAAC
CCGTTACCAGGAGCCACAGC

Downstream 100 bases:

>100_bases
CGGCGATAGCTGCTGGCGTAGACCCACGGTTTCCGGAGGGAACGCAATGAGCCAAACCGAGATGGTCACCAAACCTGTAG
CGGATGAGCGGCAGCAATCT

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MFKIITLTTLLAGWVFQTHAETLTVVSFGGLNKDAQVKAFYKPFMAAGKGTIEAGEYNGEMARIRAMVETGQIGWDIVEV
EGPELLRGCNEGLFETLDWSKLGDERQFIKGTVSQCGSGLFLWSMLLIYDASKFKTPVTSWADFWNVTTFPGKRSLRKTA
KFTMEIALLADGVKREDVYRVLATPEGVERVFNKLDQLKPNIQWWESGAQPLQWLVSGDVVMGSVYNGRVGSALKEGHDF
RMVWTDSIYDMDSWTIVKGSKKKVLAEQFIAFANLAENQKVFAENIAYGPTNINATLMIDPEIAANLPTAPANLAEAFPM
NNQFWVEHSEDLEQRFNSWAAK

Sequences:

>Translated_342_residues
MFKIITLTTLLAGWVFQTHAETLTVVSFGGLNKDAQVKAFYKPFMAAGKGTIEAGEYNGEMARIRAMVETGQIGWDIVEV
EGPELLRGCNEGLFETLDWSKLGDERQFIKGTVSQCGSGLFLWSMLLIYDASKFKTPVTSWADFWNVTTFPGKRSLRKTA
KFTMEIALLADGVKREDVYRVLATPEGVERVFNKLDQLKPNIQWWESGAQPLQWLVSGDVVMGSVYNGRVGSALKEGHDF
RMVWTDSIYDMDSWTIVKGSKKKVLAEQFIAFANLAENQKVFAENIAYGPTNINATLMIDPEIAANLPTAPANLAEAFPM
NNQFWVEHSEDLEQRFNSWAAK
>Mature_342_residues
MFKIITLTTLLAGWVFQTHAETLTVVSFGGLNKDAQVKAFYKPFMAAGKGTIEAGEYNGEMARIRAMVETGQIGWDIVEV
EGPELLRGCNEGLFETLDWSKLGDERQFIKGTVSQCGSGLFLWSMLLIYDASKFKTPVTSWADFWNVTTFPGKRSLRKTA
KFTMEIALLADGVKREDVYRVLATPEGVERVFNKLDQLKPNIQWWESGAQPLQWLVSGDVVMGSVYNGRVGSALKEGHDF
RMVWTDSIYDMDSWTIVKGSKKKVLAEQFIAFANLAENQKVFAENIAYGPTNINATLMIDPEIAANLPTAPANLAEAFPM
NNQFWVEHSEDLEQRFNSWAAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=224, Percent_Identity=26.7857142857143, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38323; Mature: 38323

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKIITLTTLLAGWVFQTHAETLTVVSFGGLNKDAQVKAFYKPFMAAGKGTIEAGEYNGE
CEEEEEHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEECCCCCCH
MARIRAMVETGQIGWDIVEVEGPELLRGCNEGLFETLDWSKLGDERQFIKGTVSQCGSGL
HHHHHHHHHCCCCCEEEEEECCHHHHHHCCHHHHHHCCHHHCCCHHHHHHHHHHHCCCHH
FLWSMLLIYDASKFKTPVTSWADFWNVTTFPGKRSLRKTAKFTMEIALLADGVKREDVYR
HHHHHHHHHCCHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEHHCCCCHHHHHH
VLATPEGVERVFNKLDQLKPNIQWWESGAQPLQWLVSGDVVMGSVYNGRVGSALKEGHDF
HHCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHCCCEEEEECCCCCHHHHHHCCCCE
RMVWTDSIYDMDSWTIVKGSKKKVLAEQFIAFANLAENQKVFAENIAYGPTNINATLMID
EEEEECCEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEEC
PEIAANLPTAPANLAEAFPMNNQFWVEHSEDLEQRFNSWAAK
CHHHCCCCCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKIITLTTLLAGWVFQTHAETLTVVSFGGLNKDAQVKAFYKPFMAAGKGTIEAGEYNGE
CEEEEEHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEECCCCCCH
MARIRAMVETGQIGWDIVEVEGPELLRGCNEGLFETLDWSKLGDERQFIKGTVSQCGSGL
HHHHHHHHHCCCCCEEEEEECCHHHHHHCCHHHHHHCCHHHCCCHHHHHHHHHHHCCCHH
FLWSMLLIYDASKFKTPVTSWADFWNVTTFPGKRSLRKTAKFTMEIALLADGVKREDVYR
HHHHHHHHHCCHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHEEEEHHCCCCHHHHHH
VLATPEGVERVFNKLDQLKPNIQWWESGAQPLQWLVSGDVVMGSVYNGRVGSALKEGHDF
HHCCCHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHCCCEEEEECCCCCHHHHHHCCCCE
RMVWTDSIYDMDSWTIVKGSKKKVLAEQFIAFANLAENQKVFAENIAYGPTNINATLMID
EEEEECCEECCCCEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEEC
PEIAANLPTAPANLAEAFPMNNQFWVEHSEDLEQRFNSWAAK
CHHHCCCCCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]