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The map label for this gene is puuE [H]

Identifier: 170023577

GI number: 170023577

Start: 1471646

End: 1472935

Strand: Direct

Name: puuE [H]

Synonym: YPK_1335

Alternate gene names: 170023577

Gene position: 1471646-1472935 (Clockwise)

Preceding gene: 170023565

Following gene: 170023578

Centisome position: 31.38

GC content: 53.33

Gene sequence:

>1290_bases
ATGAGTCACCGTGAATTGGAACAACGCAGAAAAGAGGCGACCCCACGTGGGATTGGTGTGTTATGTGATTTCTATGCGGT
ACGGGCTGAAAATGCCACGCTGTGGGATGAACAGGGTCGGGAATATATCGATTTTACGGCGGGTATTGCGACACTCAATA
TCGGACACCGCCACCCGAAAGTGATGGCCGCTGTGCGCCAACAACTGGACCAGTTTACCCATACTGCCTATCAGGTGGTG
CCTTACGCCAGTTATGTCACATTGGCAGAGAAAATTAATTCGCTAGCCCCTATTTCTGATTCAAATAAGACGGCAGCGGG
CAACAGTAAAACCGCTTTTTTCACCACTGGGGTCGAAGCAATAGAAAATGCCGTGAAAATAGCGCGTGCGGCGACTGGCC
GCCCCGGTGTGATTGCTTTCTCCGGGGCATTTCATGGCCGAACCTTATTGGCGATGGCGCTGACCGGTAGAGCGGTGCCG
TACAAAGTCGGTTTTGGTCCGTTCCCTGCCTCCATTTTTCATGCCCTCTATCCCAATGAGTTGTATGGCGTGAGTGTCGA
AGAGGCCATCAACAGTGTTGAGCGCCTGTTTCGTTGTGATATCAGTCCGACACAAGTGGCGGCCATTCTATTTGAGCCGA
TTCAGGGCGAGGGCGGTTTTAATATCGCCCCGCCGGAATTTGTCAGTGCTTTGCGCACCCTGTGTGATGAGCACGGTATT
TTGCTGATTGCCGATGAAGTACAAACGGGGTTTGCCCGTACAGGTAAGTTATTTGCCATGGAATATTATCCCGACACCAA
AGTTGATGTGATCACCATGGCGAAAAGCCTTGGGGGCGGTATGCCAATTTCCGCGGTCACGGGGCGGGCCGACATTATGG
ATGCCCCGTTGCCGGGGAGTTTGGGCGGTACTTATGCCGGTAACCCACTGGCGGTGGCTGCGTCACTGGCGGTGCTCGAT
ATTATTGCGGAAGAGAAGTTATGCGAGCGCGCCCTGATTTTAGGCGCGAAGTTGGTCGACGTACTGGAAAAAGCCCAAAT
GAGCAATGCGGCCATTGTGGGCATTCGTGCGCGTGGATCCATGGTGGCGGTGGAATTTAACGATCCCGTCAGCGGTAAAC
CGTCACCAGAACTGACGCGCGCCTATCAACGTCAGGCACTGGAAGAGGGGCTGTTACTCCTGAGTTGCGGTGTCCACAGC
AACGTGATCCGCTTCCTCTACCCATTGACCATTCCCGACAAGCAATTTAAACAGGCGATGAATATTCTTACCCGCCTACT
GGCGAGCTAA

Upstream 100 bases:

>100_bases
ATACAAATTAATCGACGAATTAGTCATTACGAACCGAGACAGTCATCCACATACGCCCCCGTGGGGGGATTGACCCATGG
CATACCGGCAGGAGCGAATC

Downstream 100 bases:

>100_bases
AGGTTTTATCTCACTGATCTCGAAACGTTAACCCGTTACCAGGAGCCACAGCATGTTTAAAATAATAACACTGACTACGC
TGCTCGCCGGATGGGTATTT

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSHRELEQRRKEATPRGIGVLCDFYAVRAENATLWDEQGREYIDFTAGIATLNIGHRHPKVMAAVRQQLDQFTHTAYQVV
PYASYVTLAEKINSLAPISDSNKTAAGNSKTAFFTTGVEAIENAVKIARAATGRPGVIAFSGAFHGRTLLAMALTGRAVP
YKVGFGPFPASIFHALYPNELYGVSVEEAINSVERLFRCDISPTQVAAILFEPIQGEGGFNIAPPEFVSALRTLCDEHGI
LLIADEVQTGFARTGKLFAMEYYPDTKVDVITMAKSLGGGMPISAVTGRADIMDAPLPGSLGGTYAGNPLAVAASLAVLD
IIAEEKLCERALILGAKLVDVLEKAQMSNAAIVGIRARGSMVAVEFNDPVSGKPSPELTRAYQRQALEEGLLLLSCGVHS
NVIRFLYPLTIPDKQFKQAMNILTRLLAS

Sequences:

>Translated_429_residues
MSHRELEQRRKEATPRGIGVLCDFYAVRAENATLWDEQGREYIDFTAGIATLNIGHRHPKVMAAVRQQLDQFTHTAYQVV
PYASYVTLAEKINSLAPISDSNKTAAGNSKTAFFTTGVEAIENAVKIARAATGRPGVIAFSGAFHGRTLLAMALTGRAVP
YKVGFGPFPASIFHALYPNELYGVSVEEAINSVERLFRCDISPTQVAAILFEPIQGEGGFNIAPPEFVSALRTLCDEHGI
LLIADEVQTGFARTGKLFAMEYYPDTKVDVITMAKSLGGGMPISAVTGRADIMDAPLPGSLGGTYAGNPLAVAASLAVLD
IIAEEKLCERALILGAKLVDVLEKAQMSNAAIVGIRARGSMVAVEFNDPVSGKPSPELTRAYQRQALEEGLLLLSCGVHS
NVIRFLYPLTIPDKQFKQAMNILTRLLAS
>Mature_428_residues
SHRELEQRRKEATPRGIGVLCDFYAVRAENATLWDEQGREYIDFTAGIATLNIGHRHPKVMAAVRQQLDQFTHTAYQVVP
YASYVTLAEKINSLAPISDSNKTAAGNSKTAFFTTGVEAIENAVKIARAATGRPGVIAFSGAFHGRTLLAMALTGRAVPY
KVGFGPFPASIFHALYPNELYGVSVEEAINSVERLFRCDISPTQVAAILFEPIQGEGGFNIAPPEFVSALRTLCDEHGIL
LIADEVQTGFARTGKLFAMEYYPDTKVDVITMAKSLGGGMPISAVTGRADIMDAPLPGSLGGTYAGNPLAVAASLAVLDI
IAEEKLCERALILGAKLVDVLEKAQMSNAAIVGIRARGSMVAVEFNDPVSGKPSPELTRAYQRQALEEGLLLLSCGVHSN
VIRFLYPLTIPDKQFKQAMNILTRLLAS

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=416, Percent_Identity=31.4903846153846, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI284507298, Length=325, Percent_Identity=32.3076923076923, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI13994255, Length=425, Percent_Identity=28.9411764705882, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI37574042, Length=424, Percent_Identity=28.3018867924528, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI24119277, Length=426, Percent_Identity=26.5258215962441, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI226442705, Length=421, Percent_Identity=27.790973871734, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI226442709, Length=397, Percent_Identity=27.9596977329975, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI188536080, Length=444, Percent_Identity=27.4774774774775, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI38679950, Length=444, Percent_Identity=27.4774774774775, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI38679946, Length=444, Percent_Identity=27.4774774774775, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1787560, Length=429, Percent_Identity=65.5011655011655, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1789016, Length=425, Percent_Identity=50.8235294117647, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1789759, Length=413, Percent_Identity=34.3825665859564, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI1788044, Length=406, Percent_Identity=33.4975369458128, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI145693181, Length=408, Percent_Identity=33.8235294117647, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1786349, Length=353, Percent_Identity=32.5779036827195, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1786991, Length=413, Percent_Identity=27.3607748184019, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25144271, Length=413, Percent_Identity=31.9612590799031, Blast_Score=184, Evalue=9e-47,
Organism=Caenorhabditis elegans, GI32564660, Length=431, Percent_Identity=32.0185614849188, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI71992977, Length=467, Percent_Identity=31.0492505353319, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI25144274, Length=291, Percent_Identity=31.9587628865979, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17541228, Length=434, Percent_Identity=25.1152073732719, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323470, Length=418, Percent_Identity=31.5789473684211, Blast_Score=197, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324432, Length=417, Percent_Identity=31.4148681055156, Blast_Score=188, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6321456, Length=419, Percent_Identity=26.2529832935561, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324386, Length=325, Percent_Identity=25.2307692307692, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21357415, Length=416, Percent_Identity=30.0480769230769, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI28574759, Length=448, Percent_Identity=28.7946428571429, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI161085790, Length=448, Percent_Identity=28.3482142857143, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21356575, Length=406, Percent_Identity=26.8472906403941, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI281366494, Length=373, Percent_Identity=27.8820375335121, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24667139, Length=373, Percent_Identity=27.8820375335121, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24667143, Length=373, Percent_Identity=27.8820375335121, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 46188; Mature: 46056

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00600 AA_TRANSFER_CLASS_3 ; PS00636 DNAJ_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHRELEQRRKEATPRGIGVLCDFYAVRAENATLWDEQGREYIDFTAGIATLNIGHRHPK
CCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHCCCEEEEECCCEEEEECCCCCHH
VMAAVRQQLDQFTHTAYQVVPYASYVTLAEKINSLAPISDSNKTAAGNSKTAFFTTGVEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHH
IENAVKIARAATGRPGVIAFSGAFHGRTLLAMALTGRAVPYKVGFGPFPASIFHALYPNE
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCC
LYGVSVEEAINSVERLFRCDISPTQVAAILFEPIQGEGGFNIAPPEFVSALRTLCDEHGI
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCE
LLIADEVQTGFARTGKLFAMEYYPDTKVDVITMAKSLGGGMPISAVTGRADIMDAPLPGS
EEEECCHHCCHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCC
LGGTYAGNPLAVAASLAVLDIIAEEKLCERALILGAKLVDVLEKAQMSNAAIVGIRARGS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
MVAVEFNDPVSGKPSPELTRAYQRQALEEGLLLLSCGVHSNVIRFLYPLTIPDKQFKQAM
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHH
NILTRLLAS
HHHHHHHCC
>Mature Secondary Structure 
SHRELEQRRKEATPRGIGVLCDFYAVRAENATLWDEQGREYIDFTAGIATLNIGHRHPK
CHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEHHCCCEEEEECCCEEEEECCCCCHH
VMAAVRQQLDQFTHTAYQVVPYASYVTLAEKINSLAPISDSNKTAAGNSKTAFFTTGVEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCHHH
IENAVKIARAATGRPGVIAFSGAFHGRTLLAMALTGRAVPYKVGFGPFPASIFHALYPNE
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHCCCC
LYGVSVEEAINSVERLFRCDISPTQVAAILFEPIQGEGGFNIAPPEFVSALRTLCDEHGI
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCE
LLIADEVQTGFARTGKLFAMEYYPDTKVDVITMAKSLGGGMPISAVTGRADIMDAPLPGS
EEEECCHHCCHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCC
LGGTYAGNPLAVAASLAVLDIIAEEKLCERALILGAKLVDVLEKAQMSNAAIVGIRARGS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
MVAVEFNDPVSGKPSPELTRAYQRQALEEGLLLLSCGVHSNVIRFLYPLTIPDKQFKQAM
EEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHH
NILTRLLAS
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]