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The map label for this gene is intS [H]

Identifier: 170022323

GI number: 170022323

Start: 69706

End: 70896

Strand: Direct

Name: intS [H]

Synonym: YPK_0061

Alternate gene names: 170022323

Gene position: 69706-70896 (Clockwise)

Preceding gene: 170022322

Following gene: 170022324

Centisome position: 1.49

GC content: 44.58

Gene sequence:

>1191_bases
ATGAAGCTAACAGTCAGACAAATCGACACAGCCAAGCCCAAAGAGAAGCCGTACAAGCTATCTGATGGTGGAGGCCTCTA
CCTTGAAGTAACCACCAACGGCTCACGCTATTGGCGTTTAAAGTATCGTTATGCGGGTAAAGAAAAGCGCCTTGCTTTCG
GCGTTTATCCTGAGGTTTCTCTCGCTCAAGCCCGTGAAAAGCGTGAAGCTGCGAAAAAGTTACTATCTGCTGGAAGCGAT
CCCGGAGAGCTAAAGAAAGCAGAGAAAATCGCTCAAAAACTAAACTACGAAAATACCTTCGAAGCTATTGCCCGTGAATG
GCACCAGCTGAGAGCGGATCGTTGGTCATTACGTTATCGCGATGAAATAATTGATACCTTTGAAAAGGATATTTTCCCCT
ATATCGGTAAGCGTCCCATAGCTGAAATTAAGCCATTAGAGCTGTTGGAAACCTTACGTAAAATGGAGAAACGCGGCGCT
TTAGAAAAGATGCGTAAAGTCCGGCAGCGCTGCGGAGAGGTTTACAGATACGCGATCATTACAGGTAGAGCGGAATACAA
TCCCGCCCCCGATCTGGCAACAGCACTAACTCCACCGAAGAAACAACACTTCCCATTCTTAACGGCTGAAGAATTGCCTT
ATTTCCTAAAAAATTTAGCTGGTTACACTGGTAGTGTTATCACCAAAACAGCGACTAAAATTATTTTGCTAACGGCAGTT
CGGACGCAAGAACTCCGCTTTACTCGTTGGCAGGATATTGATTTAGAAAAAGGTATCTGGGAAATACCTGCTGAAGTAAT
GAAGATGAAGCGACCACATGTTGTACCGTTATCTAAACAGGTGATCGAGCTATTTAATTCACTCAAACCACTGTCAGGGC
ATTATGAACTTGTCTTTATAGGTCGTAACGACCATAGAAAGCCAATCAGTAAAGAGAGCGTAAATCAGGTTATTGAGTTG
TTGGGGTATAAAGGTAGGTTAACAGGGCATGGCTTCCGCCACACGATGAGCACGATTTTGCATGAGAAGGGGTATAACTC
TGCATGGATTGAAACTCAGCTCGCGCATATTGATAAAAATGCAATTCGTGGCACGTATAACCATGCCCAGTATATGGATG
GGCGTAGAGAAATGATGCAGTGGTATGCGGATTATTTGGATGCGTTGGAGAGAGTGAAGAGGAGCAGTTAG

Upstream 100 bases:

>100_bases
AATTATGTTCTCCCGAGGTCAACTGTCTCTATCAGAATCAAGTACATTCTGGGGGCATAATGGGAGGCATGATTTATAGC
CCCATTTTGAGATGCCCCCA

Downstream 100 bases:

>100_bases
TACTAAGTATTAGTTATAGCTACTTGTCTTTAAAAGGAAAGAACAACATAGTATTACTGCAGTAATCTTATCAATAACTA
ATTTTTTATTTCTTTTATAT

Product: integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKLTVRQIDTAKPKEKPYKLSDGGGLYLEVTTNGSRYWRLKYRYAGKEKRLAFGVYPEVSLAQAREKREAAKKLLSAGSD
PGELKKAEKIAQKLNYENTFEAIAREWHQLRADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPLELLETLRKMEKRGA
LEKMRKVRQRCGEVYRYAIITGRAEYNPAPDLATALTPPKKQHFPFLTAEELPYFLKNLAGYTGSVITKTATKIILLTAV
RTQELRFTRWQDIDLEKGIWEIPAEVMKMKRPHVVPLSKQVIELFNSLKPLSGHYELVFIGRNDHRKPISKESVNQVIEL
LGYKGRLTGHGFRHTMSTILHEKGYNSAWIETQLAHIDKNAIRGTYNHAQYMDGRREMMQWYADYLDALERVKRSS

Sequences:

>Translated_396_residues
MKLTVRQIDTAKPKEKPYKLSDGGGLYLEVTTNGSRYWRLKYRYAGKEKRLAFGVYPEVSLAQAREKREAAKKLLSAGSD
PGELKKAEKIAQKLNYENTFEAIAREWHQLRADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPLELLETLRKMEKRGA
LEKMRKVRQRCGEVYRYAIITGRAEYNPAPDLATALTPPKKQHFPFLTAEELPYFLKNLAGYTGSVITKTATKIILLTAV
RTQELRFTRWQDIDLEKGIWEIPAEVMKMKRPHVVPLSKQVIELFNSLKPLSGHYELVFIGRNDHRKPISKESVNQVIEL
LGYKGRLTGHGFRHTMSTILHEKGYNSAWIETQLAHIDKNAIRGTYNHAQYMDGRREMMQWYADYLDALERVKRSS
>Mature_396_residues
MKLTVRQIDTAKPKEKPYKLSDGGGLYLEVTTNGSRYWRLKYRYAGKEKRLAFGVYPEVSLAQAREKREAAKKLLSAGSD
PGELKKAEKIAQKLNYENTFEAIAREWHQLRADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPLELLETLRKMEKRGA
LEKMRKVRQRCGEVYRYAIITGRAEYNPAPDLATALTPPKKQHFPFLTAEELPYFLKNLAGYTGSVITKTATKIILLTAV
RTQELRFTRWQDIDLEKGIWEIPAEVMKMKRPHVVPLSKQVIELFNSLKPLSGHYELVFIGRNDHRKPISKESVNQVIEL
LGYKGRLTGHGFRHTMSTILHEKGYNSAWIETQLAHIDKNAIRGTYNHAQYMDGRREMMQWYADYLDALERVKRSS

Specific function: Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788690, Length=389, Percent_Identity=50.1285347043702, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI145693166, Length=390, Percent_Identity=43.3333333333333, Blast_Score=310, Evalue=7e-86,
Organism=Escherichia coli, GI1788974, Length=390, Percent_Identity=36.9230769230769, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 46066; Mature: 46066

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTVRQIDTAKPKEKPYKLSDGGGLYLEVTTNGSRYWRLKYRYAGKEKRLAFGVYPEVS
CCEEEECCCCCCCCCCCEEECCCCEEEEEEEECCCEEEEEEEEECCCCCCEEEECCCCHH
LAQAREKREAAKKLLSAGSDPGELKKAEKIAQKLNYENTFEAIAREWHQLRADRWSLRYR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DEIIDTFEKDIFPYIGKRPIAEIKPLELLETLRKMEKRGALEKMRKVRQRCGEVYRYAII
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEE
TGRAEYNPAPDLATALTPPKKQHFPFLTAEELPYFLKNLAGYTGSVITKTATKIILLTAV
ECCCCCCCCCCHHHHCCCCCHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEHH
RTQELRFTRWQDIDLEKGIWEIPAEVMKMKRPHVVPLSKQVIELFNSLKPLSGHYELVFI
HHHHHHHCCCCCCCHHCCHHHCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEE
GRNDHRKPISKESVNQVIELLGYKGRLTGHGFRHTMSTILHEKGYNSAWIETQLAHIDKN
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AIRGTYNHAQYMDGRREMMQWYADYLDALERVKRSS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLTVRQIDTAKPKEKPYKLSDGGGLYLEVTTNGSRYWRLKYRYAGKEKRLAFGVYPEVS
CCEEEECCCCCCCCCCCEEECCCCEEEEEEEECCCEEEEEEEEECCCCCCEEEECCCCHH
LAQAREKREAAKKLLSAGSDPGELKKAEKIAQKLNYENTFEAIAREWHQLRADRWSLRYR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DEIIDTFEKDIFPYIGKRPIAEIKPLELLETLRKMEKRGALEKMRKVRQRCGEVYRYAII
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEE
TGRAEYNPAPDLATALTPPKKQHFPFLTAEELPYFLKNLAGYTGSVITKTATKIILLTAV
ECCCCCCCCCCHHHHCCCCCHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEHH
RTQELRFTRWQDIDLEKGIWEIPAEVMKMKRPHVVPLSKQVIELFNSLKPLSGHYELVFI
HHHHHHHCCCCCCCHHCCHHHCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEE
GRNDHRKPISKESVNQVIELLGYKGRLTGHGFRHTMSTILHEKGYNSAWIETQLAHIDKN
ECCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AIRGTYNHAQYMDGRREMMQWYADYLDALERVKRSS
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]