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The map label for this gene is pnp [H]

Identifier: 169833588

GI number: 169833588

Start: 632497

End: 634710

Strand: Direct

Name: pnp [H]

Synonym: SPH_0686

Alternate gene names: 169833588

Gene position: 632497-634710 (Clockwise)

Preceding gene: 169834245

Following gene: 169833198

Centisome position: 28.17

GC content: 44.63

Gene sequence:

>2214_bases
ATGGCAAAACAAGTGTTTCAAACGACTTTTGCGGGTCGTGAGTTAATTGTAGAGACTGGTCAGGTTGCTAAGCAAGCAAA
TGGCTCTGTTGTCGTACGTTACGGTGAGTCAACTGTCTTGACTGCTGCCGTTATGTCTAAGAAAATGGCAACTGGGGATT
TCTTCCCCCTCCAAGTCAACTACGAAGAAAAAATGTATGCGGCTGGGAAGTTTCCTGGTGGCTTTATGAAACGTGAAGGA
CGTCCTTCAACAGATGCGACCTTGACAGCGCGTTTGATTGACCGTCCGATTCGTCCTATGTTTGCGGAAGGTTTTCGTAA
TGAAGTCCAAGTCATCAATACAGTGCTTTCTTATGATGAAAATGCATCTGCACCAATGGCTGCTATGTTTGGTTCATCTT
TGGCACTGTCTATTTCAGATATTCCATTTGACGGACCAATTGCTGGGGTACAAGTAGGATATGTAGATGGCCAAATCATC
ATCAACCCAAGTCAAGAACAAGCAGAGCAATCTCTTCTTGAATTGACAGTAGCTGGTACCAAACACGCTATCAACATGGT
TGAGTCTGGTGCCAAAGAATTGTCAGAAGAAATCATGTTGGAAGCCCTTCTCAAAGGGCATGAAGCAGTCAAAGAATTGA
TTGCCTTCCAAGAAGAAATCGTTGCGGCAGTCGGTAAAGAAAAAGCAGAAGTGGAATTGCTTCATGTGGATGCGGACTTG
CAGGCTGAAATCATCGCAGCTTACAACAGCGACCTCCAAAAAGCGGTTCAAGTAGAAGAAAAATTAGCTCGTGAAGCTGC
AACTCAAACAGTTAAAGACCAAGTTATTGCTGTTTACGAAGAAAAATATGCAGACCACGAAGAATTTGACCGTATCATGC
GTGATGTGGCTGAAATCTTGGAACAAATGGAACACGCTGAAGTGCGCCGTTTGATTACAGAAGACAAGGTGCGTCCTGAC
GGTCGTAAGGTTGATGAAATCCGTCCTTTGGATGCGGTTGTTGACTTCCTTCCTCGTGTGCACGGTTCAGGTCTCTTCAC
TCGTGGACAAACTCAAGCTCTTTCAGTCTTGACCTTGGCTCCGATGGGTGAGACTCAAATTATCGATGGCTTGGATCCTG
AGTACAAGAAACGCTTTATGCACCACTATAACTTCCCTCAATATTCTGTAGGGGAAACTGGTCGTTACGGTGCGCCAGGT
CGTCGTGAAATCGGTCACGGTGCTCTTGGTGAGCGTGCTCTTGCTCAAGTCTTGCCAAGTTTGGAAGAATTTCCATATGC
TATCCGCTTGGTAGCTGAGGTCTTGGAATCAAATGGTTCTTCATCTCAAGCTTCTATCTGTGCGGGAACGCTTGCCCTTA
TGGCTGGTGGTGTGCCAATCAAGGCGCCAGTAGCAGGGATTGCCATGGGTCTCATCTCAGATGGAAATAACTACACAGTC
TTGACAGATATCCAAGGTTTGGAAGATCACTTTGGAGATATGGACTTTAAGGTTGCTGGTACTCGTGACGGGATTACAGC
CCTTCAAATGGATATCAAGATTCAAGGAATCACTGCAGAAATCTTGACGGAGGCTCTTGCTCAAGCCAAGAAAGCGCGTT
TTGAAATCCTTGATGTGATTGAAGCAACGATTCCAGAAGTTCGTCCAGAATTGGCTCCAACTGCTCCGAAAATTGATACC
ATCAAGATTGATGTGGACAAGATTAAGATTGTCATCGGTAAGGGTGGAGAAACCATCGACAAGATTATCGCTGAAACAGG
CGTTAAGATTGATATCGACGAAGAAGGGAATGTGTCTATCTACTCTAGCGACCAAGATGCCATTAACCGTGCTAAAGAAA
TTATTGCTGGTTTGGTCCGTGAAGCCAAAGTGGATGAAGTTTACCGTGCTAAAGTCGTTCGTATCGAGAAATTTGGTGCT
TTTGTCAACCTCTTTGATAAGACAGATGCCCTTGTTCATATCTCTGAAATGGCTTGGACGCGTACCAATAATGTCGAAGA
CTTGGTAGCAATCGGGGATGAAGTTGATGTTAAGGTTATCAAGATTGATGAAAAAGGACGTGTGGATGCTTCTATGAAAG
CCCTTCTTCCTCGTCCTCCAAAACCTGAACGTGATGAAAAAGGTGAAAAATCAGAGCGTCCTTACCGCCCACGTCATCAC
AAGGATCACAAACCTAAGAAAGAAATTACAGAAACACCAAAAGATTCAGAATAA

Upstream 100 bases:

>100_bases
GACTTCGTCAGTTTCCTATTTTTACTTTGCTTTTGACGTCCTTGGTATCTTGATCTTTGTAGGCAAGGCGTATAATTTCA
TCAATCCAAAGGGGATTAAA

Downstream 100 bases:

>100_bases
GAAAAGGAGAAATGTATGGGATGGTGGCGCGAAACCATTGATATCGTAAAAGAAAATGATCCAGCGGCCCGCACCACTTT
GGAGGTTTTGCTGACTTATC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 737; Mature: 736

Protein sequence:

>737_residues
MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREG
RPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDADL
QAEIIAAYNSDLQKAVQVEEKLAREAATQTVKDQVIAVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPD
GRKVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGETGRYGAPG
RREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTV
LTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDT
IKIDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKVDEVYRAKVVRIEKFGA
FVNLFDKTDALVHISEMAWTRTNNVEDLVAIGDEVDVKVIKIDEKGRVDASMKALLPRPPKPERDEKGEKSERPYRPRHH
KDHKPKKEITETPKDSE

Sequences:

>Translated_737_residues
MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREG
RPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDADL
QAEIIAAYNSDLQKAVQVEEKLAREAATQTVKDQVIAVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPD
GRKVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGETGRYGAPG
RREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTV
LTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDT
IKIDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKVDEVYRAKVVRIEKFGA
FVNLFDKTDALVHISEMAWTRTNNVEDLVAIGDEVDVKVIKIDEKGRVDASMKALLPRPPKPERDEKGEKSERPYRPRHH
KDHKPKKEITETPKDSE
>Mature_736_residues
AKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAVMSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGR
PSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQIII
NPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDADLQ
AEIIAAYNSDLQKAVQVEEKLAREAATQTVKDQVIAVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDG
RKVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGETGRYGAPGR
REIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTVL
TDIQGLEDHFGDMDFKVAGTRDGITALQMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTI
KIDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKVDEVYRAKVVRIEKFGAF
VNLFDKTDALVHISEMAWTRTNNVEDLVAIGDEVDVKVIKIDEKGRVDASMKALLPRPPKPERDEKGEKSERPYRPRHHK
DHKPKKEITETPKDSE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=714, Percent_Identity=34.1736694677871, Blast_Score=403, Evalue=1e-112,
Organism=Escherichia coli, GI145693187, Length=689, Percent_Identity=48.6211901306241, Blast_Score=614, Evalue=1e-177,
Organism=Caenorhabditis elegans, GI115534063, Length=715, Percent_Identity=34.2657342657343, Blast_Score=351, Evalue=7e-97,
Organism=Drosophila melanogaster, GI281362905, Length=697, Percent_Identity=36.441893830703, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651641, Length=697, Percent_Identity=36.441893830703, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24651643, Length=697, Percent_Identity=36.441893830703, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI161079377, Length=661, Percent_Identity=35.7034795763994, Blast_Score=398, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 80927; Mature: 80796

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAVMSKKMATGDFFPLQVN
CCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEC
YEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDE
CHHHHEECCCCCCCHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
NASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQIIINPSQEQAEQSLLELTVAGT
CCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHCCH
KHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
QAEIIAAYNSDLQKAVQVEEKLAREAATQTVKDQVIAVYEEKYADHEEFDRIMRDVAEIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLA
HHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEECCCHHHEEEEEEC
PMGETQIIDGLDPEYKKRFMHHYNFPQYSVGETGRYGAPGRREIGHGALGERALAQVLPS
CCCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTV
HHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEE
LTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKIQGITAEILTEALAQAKKARFEILDVI
EEECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
EATIPEVRPELAPTAPKIDTIKIDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSI
HHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHCCCEEEECCCCCEEE
YSSDQDAINRAKEIIAGLVREAKVDEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHC
RTNNVEDLVAIGDEVDVKVIKIDEKGRVDASMKALLPRPPKPERDEKGEKSERPYRPRHH
CCCCCHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
KDHKPKKEITETPKDSE
CCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
AKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAVMSKKMATGDFFPLQVN
CHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEEC
YEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFAEGFRNEVQVINTVLSYDE
CHHHHEECCCCCCCHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
NASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQIIINPSQEQAEQSLLELTVAGT
CCCCCHHHHCCCCEEEEEECCCCCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHHCCH
KHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQEEIVAAVGKEKAEVELLHVDADL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
QAEIIAAYNSDLQKAVQVEEKLAREAATQTVKDQVIAVYEEKYADHEEFDRIMRDVAEIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
EQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVVDFLPRVHGSGLFTRGQTQALSVLTLA
HHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCCEECCCHHHEEEEEEC
PMGETQIIDGLDPEYKKRFMHHYNFPQYSVGETGRYGAPGRREIGHGALGERALAQVLPS
CCCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
LEEFPYAIRLVAEVLESNGSSSQASICAGTLALMAGGVPIKAPVAGIAMGLISDGNNYTV
HHHCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCCCEEE
LTDIQGLEDHFGDMDFKVAGTRDGITALQMDIKIQGITAEILTEALAQAKKARFEILDVI
EEECCCHHHHCCCCCEEEECCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
EATIPEVRPELAPTAPKIDTIKIDVDKIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSI
HHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHCCCEEEECCCCCEEE
YSSDQDAINRAKEIIAGLVREAKVDEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWT
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHC
RTNNVEDLVAIGDEVDVKVIKIDEKGRVDASMKALLPRPPKPERDEKGEKSERPYRPRHH
CCCCCHHHHHCCCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
KDHKPKKEITETPKDSE
CCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA