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The map label for this gene is hemK [H]

Identifier: 169833726

GI number: 169833726

Start: 1034708

End: 1035547

Strand: Direct

Name: hemK [H]

Synonym: SPH_1124

Alternate gene names: 169833726

Gene position: 1034708-1035547 (Clockwise)

Preceding gene: 169833220

Following gene: 169833958

Centisome position: 46.08

GC content: 38.33

Gene sequence:

>840_bases
ATGAAATTAGCTCAATTATTTTCAAATTTTGAAGAAGAGTTGATAAGACAAGGAGAGGAAGCGGAAAGCCTCTCTTTTGT
CTATCGTAGTCTGAAAAATCTATCTTTTACAGACTTTATTTTTGCCCTCCAGCAGGAGGTGACAACAGAGGAAGAAAAAC
AATTTGTAGAAGATATTTACCAGCAACTAGCAGCTCACAAGCCAGCTCAGTACATCATTGGACAGGCAGATTTTTATGGA
ATGCATTTAAAGGTGGATGAACGGGTTTTGATTCCTCGTCCAGAAACAGAGGAGTTAGTGGAGCTTATTCTAACTGAAAA
TCTTGAGACGAATCTCTCAGTTCTGGATATTGGAACAGGTAGTGGAGCTATTGCTCTCGCTCTAGCAAAAAATAGACCAG
ATTGGTCAGTGACAGCAGCAGATGTTTCTCAAGAGGCCTTAGAGCTTGCATCAGAGAATGCTAGCAATCAAAATCTTAAT
ATATTTTTTAAAAAATCTGATTGTTTTGCAGAAATTTCTGAAAAATATGATATAATTGTATCCAATCCACCCTATATCTC
TCGTGAAGATGAGTCAGAGGTAGGCTTGAATGTTTTGCATTCGGAGCCTCATCTAGCTCTCTTTGCAGACGAGGATGGCC
TAGCTATTTACTGTAGAATTGCGGAAGATGCAAAAGACTATCTCAAGGATGGTGGTAAGATTTACCTTGAAATTGGATAC
AAGCAAGGTCAAAGTGTTCCCGAACTTTTTAGAAAACATCTTCCTGAAAAAAAAGTTCGAACACTCAAAGACCAATTTGG
TCAAAATAGGATGGTTGTGATTGATGATGGACAGGATTAG

Upstream 100 bases:

>100_bases
TCCAAAAACTAGATACGATTTTGTCTGGTAAATTGGACGAAGTTATGGATGCCTTGGTGCTTTATGACCAAACACAAAAA
CTAGAAGAATTAAACAAATA

Downstream 100 bases:

>100_bases
ACAAGAGTTGGAAAATGGTGGAGCTGTCGTTCTACCTACAGAGACTGTTTATGGTCTTTTTGCCAAGGCCTTAGATGAAA
AAGCAGTTGACCATGTTTAC

Product: protein-(glutamine-N5) methyltransferase, release factor-specific

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYG
MHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYCRIAEDAKDYLKDGGKIYLEIGY
KQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVIDDGQD

Sequences:

>Translated_279_residues
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYG
MHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYCRIAEDAKDYLKDGGKIYLEIGY
KQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVIDDGQD
>Mature_279_residues
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYG
MHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLN
IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYCRIAEDAKDYLKDGGKIYLEIGY
KQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVIDDGQD

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=264, Percent_Identity=32.9545454545455, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1787463, Length=214, Percent_Identity=36.9158878504673, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI87082085, Length=197, Percent_Identity=37.0558375634518, Blast_Score=119, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324266, Length=165, Percent_Identity=34.5454545454545, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582226, Length=186, Percent_Identity=34.9462365591398, Blast_Score=103, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31619; Mature: 31619

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
QQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTG
HHHHCCCCHHHEECCCCEEEEEEEECCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCC
SGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLNIFFKKSDCFAEISEKYDIIV
CCEEEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCCEEEE
SNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYCRIAEDAKDYLKDGGKIYLEIGY
CCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCEEEEEEHHHHHHHHHCCCEEEEEEEC
KQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVIDDGQD
CCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCC
>Mature Secondary Structure
MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQQEVTTEEEKQFVEDIY
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHH
QQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVELILTENLETNLSVLDIGTG
HHHHCCCCHHHEECCCCEEEEEEEECCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCC
SGAIALALAKNRPDWSVTAADVSQEALELASENASNQNLNIFFKKSDCFAEISEKYDIIV
CCEEEEEEECCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHCCCCEEEE
SNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYCRIAEDAKDYLKDGGKIYLEIGY
CCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCEEEEEEHHHHHHHHHCCCEEEEEEEC
KQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVIDDGQD
CCCCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]