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The map label for this gene is prfA [H]

Identifier: 169833220

GI number: 169833220

Start: 1033629

End: 1034708

Strand: Direct

Name: prfA [H]

Synonym: SPH_1123

Alternate gene names: 169833220

Gene position: 1033629-1034708 (Clockwise)

Preceding gene: 169832750

Following gene: 169833726

Centisome position: 46.03

GC content: 44.91

Gene sequence:

>1080_bases
ATGAACATCTATGATCAACTACAAGCTGTAGAAGACCGTTATGAAGAACTAGGAGAATTGCTGAGTGACCCTGATGTCGT
TTCAGACACCAGGCGTTTTATGGAGCTTTCAAAAGAAGAAGCTTCCAATCGTGACACCGTAATAGCCTACCGTGAGTATA
AACAAGTCCTTCAAAATATCGTCGATGCCGAAGAGATGATTAAGGAATCAGGCGGAGATGCGGACTTGGAAGAAATGGCC
AAGCAAGAACTCAAAGATGCCAAGGCTGAAAAAGAAGAATATGAAGAAAAACTGAAAATTTTGCTCCTTCCAAAGGATCC
AAACGATGACAAGAATATCATCCTTGAAATCCGTGGAGCAGCTGGTGGAGACGAAGCGGCACTTTTCGCTGGAGATTTGC
TAACTATGTACCAAAAGTATGCGGAAGCCCAAGGTTGGCGCTTTGAAGTCATGGAAGCCTCTATGAATGGTGTCGGTGGT
TTTAAAGAAGTGGTTGCTATGGTTTCAGGTCAGTCTGTATACTCTAAGCTTAAGTATGAATCAGGTGCCCACCGTGTGCA
ACGTGTTCCTGTGACAGAAAGCCAAGGCCGTGTTCATACTTCGACAGCGACAGTTCTTGTTATGCCAGAAGTTGAAGAGG
TTGAATACGACATTGATCCAAAAGACCTTCGTGTCGACATCTATCACGCCTCTGGTGCTGGTGGACAGAACGTCAATAAG
GTTGCGACTGCCGTTCGTATTGTTCACTTGCCAACCAATATCAAGGTTGAGATGCAGGAAGAACGTACCCAGCAGAAGAA
CCGTGAGAAGGCTATGAAGATTATCCGTGCACGCGTCGCTGACCACTTTGCTCAGATTGCTCAGGATGAACAAGACGCTG
AGCGTAAGTCGACAATCGGTACTGGTGACCGTTCAGAACGGATCCGAACTTATAACTTCCCACAAAACCGTGTCACAGAC
CACCGTATCGGCTTGACCCTCCAAAAACTAGATACGATTTTGTCTGGTAAATTGGACGAAGTTATGGATGCCTTGGTGCT
TTATGACCAAACACAAAAACTAGAAGAATTAAACAAATAA

Upstream 100 bases:

>100_bases
ATTTTGTTACCTCAGTTATGGATTTAAAGAAACTGGGGAAATATCCGAGAATGAAGTAGTAGCAATCTATGATTTAACAA
TTGAGAAATAAGGAGAAAAA

Downstream 100 bases:

>100_bases
TGAAATTAGCTCAATTATTTTCAAATTTTGAAGAAGAGTTGATAAGACAAGGAGAGGAAGCGGAAAGCCTCTCTTTTGTC
TATCGTAGTCTGAAAAATCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MNIYDQLQAVEDRYEELGELLSDPDVVSDTRRFMELSKEEASNRDTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMA
KQELKDAKAEKEEYEEKLKILLLPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
VATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIRARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTD
HRIGLTLQKLDTILSGKLDEVMDALVLYDQTQKLEELNK

Sequences:

>Translated_359_residues
MNIYDQLQAVEDRYEELGELLSDPDVVSDTRRFMELSKEEASNRDTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMA
KQELKDAKAEKEEYEEKLKILLLPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
VATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIRARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTD
HRIGLTLQKLDTILSGKLDEVMDALVLYDQTQKLEELNK
>Mature_359_residues
MNIYDQLQAVEDRYEELGELLSDPDVVSDTRRFMELSKEEASNRDTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMA
KQELKDAKAEKEEYEEKLKILLLPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
VATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIRARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTD
HRIGLTLQKLDTILSGKLDEVMDALVLYDQTQKLEELNK

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=45.4861111111111, Blast_Score=258, Evalue=4e-69,
Organism=Homo sapiens, GI34577120, Length=347, Percent_Identity=37.463976945245, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI166795305, Length=181, Percent_Identity=45.3038674033149, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1787462, Length=361, Percent_Identity=49.3074792243767, Blast_Score=350, Evalue=7e-98,
Organism=Escherichia coli, GI2367172, Length=347, Percent_Identity=35.7348703170029, Blast_Score=191, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI17542784, Length=284, Percent_Identity=38.0281690140845, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=323, Percent_Identity=41.4860681114551, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI19921226, Length=318, Percent_Identity=40.5660377358491, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40690; Mature: 40690

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIYDQLQAVEDRYEELGELLSDPDVVSDTRRFMELSKEEASNRDTVIAYREYKQVLQNI
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
VDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILLLPKDPNDDKNIILEIRGA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEECC
AGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGGFKEVVAMVSGQSVYSKLKYE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCHHHHHHHHCCHHHHHHHHHC
SGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
CCCHHHEECCCCCCCCEEEECCEEEEEECCHHHCCCCCCCCCEEEEEEEECCCCCCCHHH
VATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIRARVADHFAQIAQDEQDAERKSTIG
HHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
TGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTILSGKLDEVMDALVLYDQTQKLEELNK
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIYDQLQAVEDRYEELGELLSDPDVVSDTRRFMELSKEEASNRDTVIAYREYKQVLQNI
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHH
VDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILLLPKDPNDDKNIILEIRGA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCEEEEEEECC
AGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGGFKEVVAMVSGQSVYSKLKYE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHCCCCCCHHHHHHHHCCHHHHHHHHHC
SGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDIDPKDLRVDIYHASGAGGQNVNK
CCCHHHEECCCCCCCCEEEECCEEEEEECCHHHCCCCCCCCCEEEEEEEECCCCCCCHHH
VATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIRARVADHFAQIAQDEQDAERKSTIG
HHHHHEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCC
TGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTILSGKLDEVMDALVLYDQTQKLEELNK
CCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA