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The map label for this gene is pyrF [H]

Identifier: 169833557

GI number: 169833557

Start: 737908

End: 738609

Strand: Direct

Name: pyrF [H]

Synonym: SPH_0785

Alternate gene names: 169833557

Gene position: 737908-738609 (Clockwise)

Preceding gene: 169834178

Following gene: 169833203

Centisome position: 32.86

GC content: 45.58

Gene sequence:

>702_bases
ATGCGAGAACATCGTCCAATCATTGCTCTTGATTTTCCTAGTTTTGAGGTGGTCAAGGAATTTTTAGCTCTTTTCCCAGC
AGAAGAAAGCCTTTATCTCAAGGTAGGGATGGAGCTTTATTACGCAGCGGGGCCTGAGATTGTGTCCTACTTAAAAGGTT
TGGGTCATAGTGTCTTTTTGGATCTCAAACTTCATGACATTCCCAATACAGTCAAGTCAGCAATGAAGATCCTATCTCAG
CTTGGTGTGGATATGACCAATGTTCATGCGGCTGGTGGTGTAGAGATGATGAAGGCTGCGCGTGAAGGTCTTGGAAGTCA
AGCTAAATTGATCGCTGTAACTCAGCTCACATCAACGTCAGAAGCTCAGATGCAGGAGTTTCAAAATATCCAAACCAGTC
TGCAAGAGTCTGTGATTCACTATGCCAAGAAGACAGCTGAAGCTGGCTTGGATGGTGTTGTTTGCTCGGCTCAGGAAGTA
CAAGTCATCAAGCAGGCTACCAATCCAGATTTTATCTGTCTGACACCAGGGATTCGTCCAGCTGGTGTTGCAGTTGGAGA
TCAAAAACGAGTCATGACACCTGCTGATGCCTATCAAATCGGCAGTGACTATATCGTAGTGGGACGTCCCATTACCCAAG
CTGAGGATCCTGTTGCAGCTTATCATGCCATCAAGGATGAATGGACACAGGACTGGAATTAA

Upstream 100 bases:

>100_bases
GACTACCCATTTTTCGTGGAACCTTGCTGTTGGCAGGTTCCTTTTTTCGTGGCTTCTGTTGGCCAGACTCTCTCACTAGT
AAAGGTAAAAGGAGAAACCT

Downstream 100 bases:

>100_bases
AGAACTAGATTAGAAAAATAAAAGGAGAATACCATGACACTTGCTAAAGATATCGCTAGCCACCTCTTGAAAATCCAAGC
CGTTTACCTCAAACCAGAGG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MREHRPIIALDFPSFEVVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQ
LGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPVAAYHAIKDEWTQDWN

Sequences:

>Translated_233_residues
MREHRPIIALDFPSFEVVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQ
LGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPVAAYHAIKDEWTQDWN
>Mature_233_residues
MREHRPIIALDFPSFEVVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQ
LGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPVAAYHAIKDEWTQDWN

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=223, Percent_Identity=43.9461883408072, Blast_Score=166, Evalue=9e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25451; Mature: 25451

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREHRPIIALDFPSFEVVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFL
CCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEECEEEEECCCHHHHHHHHHCCCEEEE
DLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTS
EEEECCCCHHHHHHHHHHHHHCCCHHHEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
EAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEVQVIKQATNPDFICLTPGIRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCEEEECCCCCC
AGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPVAAYHAIKDEWTQDWN
CCEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MREHRPIIALDFPSFEVVKEFLALFPAEESLYLKVGMELYYAAGPEIVSYLKGLGHSVFL
CCCCCCEEEEECCCHHHHHHHHHHCCCCCCEEEEECEEEEECCCHHHHHHHHHCCCEEEE
DLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAAREGLGSQAKLIAVTQLTSTS
EEEECCCCHHHHHHHHHHHHHCCCHHHEECCCCHHHHHHHHHCCCCCCEEEEEEECCCCC
EAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEVQVIKQATNPDFICLTPGIRP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHCCCCCEEEECCCCCC
AGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPVAAYHAIKDEWTQDWN
CCEEECCCCCCCCCHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA