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The map label for this gene is dhaK2 [H]

Identifier: 169832984

GI number: 169832984

Start: 1200325

End: 1201314

Strand: Direct

Name: dhaK2 [H]

Synonym: SPH_1294

Alternate gene names: 169832984

Gene position: 1200325-1201314 (Clockwise)

Preceding gene: 169833473

Following gene: 169834064

Centisome position: 53.45

GC content: 38.38

Gene sequence:

>990_bases
ATGACATTTATTTCAAATCATAAACAGAAAATTATTTCAAGTTACATTTCGGCAACTGTCAGCAGTCATCCTGAATTAGA
AAAACACCCAACCTTACCTTTAGTCTATCAAAAAAATCGCAATCCTCATCAGGTTCCAATATTGTCGGGTGGAGGTTCAG
GTCACGAACCTGCTCATATTGGATATGTGGGAGAAGGAATGCTTACTGCTGCTATCTATGGCCAATTATTTACTCCTCCC
ACAAGAACTGAAATCTTAGAGTCCATTCGTTTTTTAAACAATGGTCACGGTGTCTTCATCATTGTAAAAAATTTCGAAGC
AGACATCAAAGAATTTAGCTCGGCCATTAACACTGCTAGAAAAGAAGGAATTAAAGTTGGCTATAGTCTAGCACACGACG
ATATTTCAATTGAACCTCACAATAGATTTCAAATTAGAGGAAGAGGGCTTGCAGGGACTATTTTACTTCATAAAGTCCTA
GGATATGCAGCTCAGAATGGTGCCGACATCGAGCAACTAACTGATTTAGGTCATCAGCTTGCTCCCGAAATCGCAACAAT
TGGCTTTGCAACGAAATCTGCTAGTCTCCCCCAAGCCGCCCTTCCACTATTTAACTTGGAAGAAGGAAATATTTCTTATG
GTATTGGGATACATGGCGAAGAAGGTTATCGTATCGTCCCATTCCAATCATCGGAAATCCTTGCAAATGAAATTATAAGT
AAATTAAGATTGCACTATCATTGGAAAAAGGGTGATCAATTTATATTGCTTGTAAATAATCTAGGCACTACCAGCAACCT
AGAAATGGGGATATTTATCAATGATCTTCTTCAACTCTTAGAAATTGAAGGAGTCACTATTACCTTTATAAAATCAGGAA
CATTTATGACCAGTTTGGATATGGCAGGTATATCCGTAACTCTTTGCCCTGTAAAAAATAAGCAGTGGCTAGAAGCGCTC
AATGCTCCAACGACAGCTTTTGCATGGTAA

Upstream 100 bases:

>100_bases
CACATGCTCTAGCAGAAATCGTGAAGGCTTATGTCAATAAAAAAAGTTCAGTTCCACAACCAGACTTTCTCATCATGACA
ATTATTGGAAAGAGACCACA

Downstream 100 bases:

>100_bases
TTTGCTTGTATAACTCAAAAGGGCTACATCACTTGATAGCCCTTTGCTTTTATCTTACGTTTAAAAGAACTGATTATTAC
CATTTCAAATAGGCCATCTA

Product: DhaKLM operon coactivator DhaQ

Products: NA

Alternate protein names: DhaS coactivator dhaQ [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MTFISNHKQKIISSYISATVSSHPELEKHPTLPLVYQKNRNPHQVPILSGGGSGHEPAHIGYVGEGMLTAAIYGQLFTPP
TRTEILESIRFLNNGHGVFIIVKNFEADIKEFSSAINTARKEGIKVGYSLAHDDISIEPHNRFQIRGRGLAGTILLHKVL
GYAAQNGADIEQLTDLGHQLAPEIATIGFATKSASLPQAALPLFNLEEGNISYGIGIHGEEGYRIVPFQSSEILANEIIS
KLRLHYHWKKGDQFILLVNNLGTTSNLEMGIFINDLLQLLEIEGVTITFIKSGTFMTSLDMAGISVTLCPVKNKQWLEAL
NAPTTAFAW

Sequences:

>Translated_329_residues
MTFISNHKQKIISSYISATVSSHPELEKHPTLPLVYQKNRNPHQVPILSGGGSGHEPAHIGYVGEGMLTAAIYGQLFTPP
TRTEILESIRFLNNGHGVFIIVKNFEADIKEFSSAINTARKEGIKVGYSLAHDDISIEPHNRFQIRGRGLAGTILLHKVL
GYAAQNGADIEQLTDLGHQLAPEIATIGFATKSASLPQAALPLFNLEEGNISYGIGIHGEEGYRIVPFQSSEILANEIIS
KLRLHYHWKKGDQFILLVNNLGTTSNLEMGIFINDLLQLLEIEGVTITFIKSGTFMTSLDMAGISVTLCPVKNKQWLEAL
NAPTTAFAW
>Mature_328_residues
TFISNHKQKIISSYISATVSSHPELEKHPTLPLVYQKNRNPHQVPILSGGGSGHEPAHIGYVGEGMLTAAIYGQLFTPPT
RTEILESIRFLNNGHGVFIIVKNFEADIKEFSSAINTARKEGIKVGYSLAHDDISIEPHNRFQIRGRGLAGTILLHKVLG
YAAQNGADIEQLTDLGHQLAPEIATIGFATKSASLPQAALPLFNLEEGNISYGIGIHGEEGYRIVPFQSSEILANEIISK
LRLHYHWKKGDQFILLVNNLGTTSNLEMGIFINDLLQLLEIEGVTITFIKSGTFMTSLDMAGISVTLCPVKNKQWLEALN
APTTAFAW

Specific function: Coactivator for the transcription factor dhaS. The heterotetramer formed by dhaQ and dhaS functions as transcriptional regulator. Activated by covalent binding of dihydroxyacetone to dhaQ. The complex activates the dhaKLM operon [H]

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=301, Percent_Identity=38.8704318936877, Blast_Score=181, Evalue=6e-46,
Organism=Escherichia coli, GI87081857, Length=357, Percent_Identity=33.3333333333333, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17565018, Length=326, Percent_Identity=31.9018404907975, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321055, Length=345, Percent_Identity=30.4347826086957, Blast_Score=152, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6323570, Length=351, Percent_Identity=30.1994301994302, Blast_Score=152, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012735 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: NA

Molecular weight: Translated: 36080; Mature: 35949

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFISNHKQKIISSYISATVSSHPELEKHPTLPLVYQKNRNPHQVPILSGGGSGHEPAHI
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCEE
GYVGEGMLTAAIYGQLFTPPTRTEILESIRFLNNGHGVFIIVKNFEADIKEFSSAINTAR
EEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHH
KEGIKVGYSLAHDDISIEPHNRFQIRGRGLAGTILLHKVLGYAAQNGADIEQLTDLGHQL
HCCCEECEEEECCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
APEIATIGFATKSASLPQAALPLFNLEEGNISYGIGIHGEEGYRIVPFQSSEILANEIIS
CCHHHHEEEECCCCCCCHHHCCEEECCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHH
KLRLHYHWKKGDQFILLVNNLGTTSNLEMGIFINDLLQLLEIEGVTITFIKSGTFMTSLD
HHHHEEEEECCCEEEEEEECCCCCCCEEEEEEHHHHHHHHHCCCEEEEEEECCCEEEEEE
MAGISVTLCPVKNKQWLEALNAPTTAFAW
CCCCEEEEEECCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TFISNHKQKIISSYISATVSSHPELEKHPTLPLVYQKNRNPHQVPILSGGGSGHEPAHI
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEEEECCCCCCCCCEE
GYVGEGMLTAAIYGQLFTPPTRTEILESIRFLNNGHGVFIIVKNFEADIKEFSSAINTAR
EEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHH
KEGIKVGYSLAHDDISIEPHNRFQIRGRGLAGTILLHKVLGYAAQNGADIEQLTDLGHQL
HCCCEECEEEECCCEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
APEIATIGFATKSASLPQAALPLFNLEEGNISYGIGIHGEEGYRIVPFQSSEILANEIIS
CCHHHHEEEECCCCCCCHHHCCEEECCCCCEEEEEEEECCCCCEEEECCCCHHHHHHHHH
KLRLHYHWKKGDQFILLVNNLGTTSNLEMGIFINDLLQLLEIEGVTITFIKSGTFMTSLD
HHHHEEEEECCCEEEEEEECCCCCCCEEEEEEHHHHHHHHHCCCEEEEEEECCCEEEEEE
MAGISVTLCPVKNKQWLEALNAPTTAFAW
CCCCEEEEEECCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]