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The map label for this gene is proB [H]

Identifier: 169832926

GI number: 169832926

Start: 961118

End: 962227

Strand: Direct

Name: proB [H]

Synonym: SPH_1040

Alternate gene names: 169832926

Gene position: 961118-962227 (Clockwise)

Preceding gene: 169832880

Following gene: 169832615

Centisome position: 42.8

GC content: 44.23

Gene sequence:

>1110_bases
ATGAAATACAAACGGATTGTCTTTAAGGTGGGTACTTCTTCTCTGACAAATGAGGATGGAAGTTTATCACGTAGTAAGGT
AAAGGATATTACCCAGCAGTTGGCTATGCTGCACGAGGCTGGTCATGAGTTGATTTTGGTGTCTTCAGGTGCCATTGCGG
CTGGTTTTGGAGCCTTAGGATTTAAAAAGCGTCCGACTAAGATTGCTGATAAACAGGCTTCAGCAGCGGTAGGGCAAGGG
CTTTTGTTGGAAGAATATACAACCAATCTTCTCTTGCGTCAAATCGTTTCTGCACAAATCTTGCTGACCCAAGATGATTT
TGTGGATAAGCGTCGTTATAAAAATGCCCATCAGGCTTTGTCGGTTTTACTCAACCGTGGGGCAATTCCTATCATCAATG
AGAATGATAGTGTCGTTATTGATGAGGTCAAGGTTGGGGACAATGACACTCTAAGTGCCCAAGTAGCGGCGATGGTCCAA
GCAGACCTTTTGGTTCTCTTGACAGATGTGGACGGTCTCTATACTGGAAATCCTAATTCAGATCCAAGAGCCAAACGCTT
GGAGAGAATAGAGACCATCAATCGTGAGATTATTGATATGGCTGGTGGAGCTGGTTCGTCAAACGGAACTGGGGGTATGT
TAACCAAAATCAAGGCTGCAACTATCGCGACGGAATCAGGAGTTCCTGTTTATATCTGCTCATCCTTGAAATCAGATTCC
ATGATTGAGGCGGCAGAGGAGACCGAGGATGGTTCTTACTTTGTTGCTCAAGAGAAGGGGCTTCGTACCCAGAAACAATG
GCTTGCCTTCTATGCTCAGAGTCAAGGTTCTATTTGGGTTGATAAAGGGGCTGCGGAAGCTCTCTCTCAACATGGAAAGA
GTCTTCTCTTATCTGGTATTGTTGAAGCAGAAGGAGCCTTTTCTTACGGTGATATCGTGACAGTATTTGACAAGGAAAGT
GGAAAATCACTTGGAAAAGGACGCGTGCAATTTGGAGCATCTGCTTTGGAGGATATATTGCGTTCTCAAAAAGCCAAGGG
TGTCTTGATTTACCGTGACGACTGGATTTCCATTACTCCTGAAATCCAACTACTTTTTACAGAATTTTAG

Upstream 100 bases:

>100_bases
TAGAAAAGAAGGTTTTAGGGCCTTCTTTTTCCTATCAACTCTTTTCTATTTCCTGTTATTCATGTTATAATGGATAAATA
TGAATAATCGGAGTGAGACT

Downstream 100 bases:

>100_bases
AGGTAAACTATGGTGAGTAGACAAGAACAATTTGAACAGGTACAGGCTGTTAAAAAATCGATTAACACAGCTAGTGAAGA
AGTGAAAAATCAAGCCTTGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQG
LLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSAQVAAMVQ
ADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKES
GKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF

Sequences:

>Translated_369_residues
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQG
LLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSAQVAAMVQ
ADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKES
GKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF
>Mature_369_residues
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQG
LLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSAQVAAMVQ
ADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKES
GKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITPEIQLLFTEF

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=260, Percent_Identity=32.3076923076923, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI62912457, Length=258, Percent_Identity=32.5581395348837, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1786437, Length=362, Percent_Identity=38.6740331491713, Blast_Score=234, Evalue=6e-63,
Organism=Caenorhabditis elegans, GI17569967, Length=270, Percent_Identity=34.0740740740741, Blast_Score=131, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17569969, Length=270, Percent_Identity=34.0740740740741, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320506, Length=410, Percent_Identity=34.8780487804878, Blast_Score=199, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6321822, Length=268, Percent_Identity=39.1791044776119, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=33.7979094076655, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39808; Mature: 39808

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALG
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCC
FKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQAL
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHH
SVLLNRGAIPIINENDSVVIDEVKVGDNDTLSAQVAAMVQADLLVLLTDVDGLYTGNPNS
HHHHHCCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCEECCCCCC
DPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEEECCCCCEEEECCCCCCC
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGI
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHCCHHHHHHHH
VEAEGAFSYGDIVTVFDKESGKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITP
HHCCCCCCCCCEEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHCCCEEEEEECCCEEECC
EIQLLFTEF
CEEEEEECC
>Mature Secondary Structure
MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALG
CCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCC
FKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQAL
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHH
SVLLNRGAIPIINENDSVVIDEVKVGDNDTLSAQVAAMVQADLLVLLTDVDGLYTGNPNS
HHHHHCCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHCEEEEEECCCCEECCCCCC
DPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDS
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEEEEEEEEECCCCCEEEECCCCCCC
MIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGI
HHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHCCHHHHHHHH
VEAEGAFSYGDIVTVFDKESGKSLGKGRVQFGASALEDILRSQKAKGVLIYRDDWISITP
HHCCCCCCCCCEEEEEECCCCCCCCCCHHHCCHHHHHHHHHHHCCCEEEEEECCCEEECC
EIQLLFTEF
CEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA