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The map label for this gene is nanE [H]

Identifier: 169832454

GI number: 169832454

Start: 769060

End: 769653

Strand: Direct

Name: nanE [H]

Synonym: SPH_0824

Alternate gene names: 169832454

Gene position: 769060-769653 (Clockwise)

Preceding gene: 169832531

Following gene: 169834130

Centisome position: 34.25

GC content: 35.69

Gene sequence:

>594_bases
GTGATGCCGTTATTAGCAAAAGCCGCCTTTCAAGCAGGCGCTAAAGGTATTCGTGCTAATTCTGTCCGCGATATTTCAGA
AATAAAAGAAGAAGTTGACTTACCAATTATAGGTATCATAAAAAGAGATTACGATGGCTTTGAACCTTTTATTTCAGCAA
CTATGAAAGAGATTGATGAATTAGTTAGTGAAGGAGTAGACATTTTAGCTTTGGATTGTACTAATCGTTCAAGACCAGGT
TATGATAATATTACTGACTTTATTCATGATATTAAAGTCAAGTATCCAAACAAATTATTAATGGCTGATATTTCTACTTT
TGAAGAAGGGAAAGTAGCTGTTGAATCGGGAGTAGATTTTGTGGGTACGACATTATCTGGTTATACACCATATAGCCCTA
AAAAAGATAATCCTGATTTTGAGTTAGTTGAAAGATTGGTAAAAGAGCTTGACGTACCAGTTATTGCTGAAGGAAGAATA
AGTACTCCGGAACAAGCTAGAAAAATGTTAGATTTAGGTGCTTACGCAGTGGTTGTTGGAGGAGCTATCACTCGTCCTTT
GGAAATAGCGAAGAAGTTTATTGAGGTAGTTTAG

Upstream 100 bases:

>100_bases
TTCTGAATGGAAACTAAAAAAATTAAAAAATTTAAAAGGTCAAATAATTGTTTCCTGCCAAGCGTTAGAAGGAGAGCCAC
TTTATACTCCAAATGGAGGA

Downstream 100 bases:

>100_bases
ATGAATCAAGAAATATTAAAAAAGAGTAAGATTCAATTGACAGTTGAACATATTCAAAAATATTGGATTTTATATTTAAT
GATGATTCCAGCAACTGTTC

Product: NanE/NanK bifunctional enzyme

Products: NA

Alternate protein names: ManNAc-6-P epimerase [H]

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MMPLLAKAAFQAGAKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILALDCTNRSRPG
YDNITDFIHDIKVKYPNKLLMADISTFEEGKVAVESGVDFVGTTLSGYTPYSPKKDNPDFELVERLVKELDVPVIAEGRI
STPEQARKMLDLGAYAVVVGGAITRPLEIAKKFIEVV

Sequences:

>Translated_197_residues
MMPLLAKAAFQAGAKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILALDCTNRSRPG
YDNITDFIHDIKVKYPNKLLMADISTFEEGKVAVESGVDFVGTTLSGYTPYSPKKDNPDFELVERLVKELDVPVIAEGRI
STPEQARKMLDLGAYAVVVGGAITRPLEIAKKFIEVV
>Mature_197_residues
MMPLLAKAAFQAGAKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILALDCTNRSRPG
YDNITDFIHDIKVKYPNKLLMADISTFEEGKVAVESGVDFVGTTLSGYTPYSPKKDNPDFELVERLVKELDVPVIAEGRI
STPEQARKMLDLGAYAVVVGGAITRPLEIAKKFIEVV

Specific function: Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) (Potential) [H]

COG id: COG3010

COG function: function code G; Putative N-acetylmannosamine-6-phosphate epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nanE family [H]

Homologues:

Organism=Escherichia coli, GI1789617, Length=183, Percent_Identity=39.8907103825137, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR007260
- InterPro:   IPR011060 [H]

Pfam domain/function: PF04131 NanE [H]

EC number: =5.1.3.9 [H]

Molecular weight: Translated: 21679; Mature: 21679

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPLLAKAAFQAGAKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDE
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
LVSEGVDILALDCTNRSRPGYDNITDFIHDIKVKYPNKLLMADISTFEEGKVAVESGVDF
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEHHCCCCH
VGTTLSGYTPYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVG
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHC
GAITRPLEIAKKFIEVV
CHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMPLLAKAAFQAGAKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDE
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
LVSEGVDILALDCTNRSRPGYDNITDFIHDIKVKYPNKLLMADISTFEEGKVAVESGVDF
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHCCCCEEHHCCCCH
VGTTLSGYTPYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVG
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHC
GAITRPLEIAKKFIEVV
CHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12066186; 12663927 [H]