Click here to switch to the map view.

The map label for this gene is rnjA [H]

Identifier: 169824328

GI number: 169824328

Start: 696228

End: 697895

Strand: Direct

Name: rnjA [H]

Synonym: FMG_0631

Alternate gene names: 169824328

Gene position: 696228-697895 (Clockwise)

Preceding gene: 169824327

Following gene: 169824329

Centisome position: 38.73

GC content: 32.31

Gene sequence:

>1668_bases
ATGGCAAAAAAGAATATTGATAAATTAAAAGTTATTCCACTAGGAGGACTTAATGAAATAGGGAAAAATATGACAGTAGT
AGAATACAAAGATGATATTATTGTCGTTGATTGTGGAATGACATTTCCTGATGAAGAAATGTTGGGGGTAGATATAGTAA
TCCCTGATATTTCTTATCTAGAAAAAAATAAGGAAAAGATCAGAGGAATAGTTATAACTCACGGTCACGAAGACCATATT
GGAGCAATTCCTTATGTATTAAAAAAATTAGATGCCCCTGTTTATGGAACAAAACTTACAATGGGACTGTTAGAAAATAA
AATATTGGAGCACAAGTTAAGTTTGAAGTCACTTCATACAGTTGATTACAGAAAACCAATTAAACTTGGTGCTTTTAGCG
TAGAGTTTGTAAAAGTATGTCACTCAATTCCAGATTCTGCAAGTTTAGCTATAACAACTCCTGTAGGAGTTTTGTTCTTT
ACTGGTGATTTTAAAATAGACTACACTCCAATTGATAACAATAGAATGGATTTTGGAAGAATAGCTTCCATAGGTAATAA
GGGAGTTCTAGCACTATTCGCTGATAGTACAAATGTTGAAAGACAAGGTTATACTTTAAGTGAAAAAAGTGTTGGTAAAA
CTTTTATAAATTTGTTTGACGATGCACCTGCTAGAATAATTGTAGCGACCTTTGCATCTAATGTTCACAGAATTCAACAA
GTTATTTCAGCTGCTGAGCATTTCAATAAAAAAGTAGCTCTTTCAGGAAGGTCTATGATTAACACTGTTAACGTTGCGCG
TGAGTTAGGATACATTAAAGTTGGCGACAAAACTATTATTGATATAAATGATATCAATAAGCATAAACCAAACGAAATAG
TTCTTTTAACAACTGGTTCTCAAGGTGAACCAATGAGTGCAATGACGAGAATGGCTAATGGTACACACAGAAAAGTTCAT
TTAGATCCTACTGATACAGTTATTTTGTCTGCTACACCAATTCCAGGAAATGATAATCAAGTTAGTTCAGTTATCAATAA
ATTGATGGAAATGGGAGTTAAAGTTATTTATTCTTCCTTGGCTGATGTACACGTAAGTGGACACGCTTGTCAAGAAGAAC
TGAAATTGATGCATTCATTAGTTAGACCTAAATTTTTTGTTCCACTTCATGGAGAAATGAGACACTTAAAGTGTCATGCA
AATTTAGCACTTGATATGGGAATGAAAGAAAAAGATATTGTGATTGCAGACAATGGTAATACAATTGAATTTACAAGAAA
AAGCATTAATCTTGTCGACACTAAATTTGCAAGTAACATTTTAGTAGATGGACTTGGAGTAGGTGATGTTGGAAATGTTG
TATTAAGAGACAGAAGACATTTATCAGAAGACGGATTAATTGTTGTTGTAATTACTTTGGATGCTAGAAACAAAGAAGTT
ATAGCTGGTCCTGACATCATTTCAAGAGGATTTGTATATGTTAAAGAAAATAATGATTTAATGGAAGAGTGCAAATCTGT
TGTTCAAAATGTTTTAGACGAATGTTACACGAAAAATATCTACGATTGGTCAACTCTTAAACACAATATTAGAGAAACAT
TAAAGAAATTCTTGTACCAAGAAATCAAGAGAAATCCAATGATATTGCCAGTAATAATGGAGGTATAA

Upstream 100 bases:

>100_bases
AATTAGAAAAGATTACAACTTCGATATACAAAATCACGATGTAAAGTTTTATGGGATATGTTCTGATTGCCAAGAGAAAC
TTAAAAATGAGGAGAAATAA

Downstream 100 bases:

>100_bases
TATGGAATATAAGAAAAAAGCTGAAGAATTAGCTCAAATGTTGAATAATTCATCAGAGTTTCAAGAACTTAAAAAACTTC
ACAAAGATGTTTATAAAAAG

Product: putative Zn-dependent hydrolase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MAKKNIDKLKVIPLGGLNEIGKNMTVVEYKDDIIVVDCGMTFPDEEMLGVDIVIPDISYLEKNKEKIRGIVITHGHEDHI
GAIPYVLKKLDAPVYGTKLTMGLLENKILEHKLSLKSLHTVDYRKPIKLGAFSVEFVKVCHSIPDSASLAITTPVGVLFF
TGDFKIDYTPIDNNRMDFGRIASIGNKGVLALFADSTNVERQGYTLSEKSVGKTFINLFDDAPARIIVATFASNVHRIQQ
VISAAEHFNKKVALSGRSMINTVNVARELGYIKVGDKTIIDINDINKHKPNEIVLLTTGSQGEPMSAMTRMANGTHRKVH
LDPTDTVILSATPIPGNDNQVSSVINKLMEMGVKVIYSSLADVHVSGHACQEELKLMHSLVRPKFFVPLHGEMRHLKCHA
NLALDMGMKEKDIVIADNGNTIEFTRKSINLVDTKFASNILVDGLGVGDVGNVVLRDRRHLSEDGLIVVVITLDARNKEV
IAGPDIISRGFVYVKENNDLMEECKSVVQNVLDECYTKNIYDWSTLKHNIRETLKKFLYQEIKRNPMILPVIMEV

Sequences:

>Translated_555_residues
MAKKNIDKLKVIPLGGLNEIGKNMTVVEYKDDIIVVDCGMTFPDEEMLGVDIVIPDISYLEKNKEKIRGIVITHGHEDHI
GAIPYVLKKLDAPVYGTKLTMGLLENKILEHKLSLKSLHTVDYRKPIKLGAFSVEFVKVCHSIPDSASLAITTPVGVLFF
TGDFKIDYTPIDNNRMDFGRIASIGNKGVLALFADSTNVERQGYTLSEKSVGKTFINLFDDAPARIIVATFASNVHRIQQ
VISAAEHFNKKVALSGRSMINTVNVARELGYIKVGDKTIIDINDINKHKPNEIVLLTTGSQGEPMSAMTRMANGTHRKVH
LDPTDTVILSATPIPGNDNQVSSVINKLMEMGVKVIYSSLADVHVSGHACQEELKLMHSLVRPKFFVPLHGEMRHLKCHA
NLALDMGMKEKDIVIADNGNTIEFTRKSINLVDTKFASNILVDGLGVGDVGNVVLRDRRHLSEDGLIVVVITLDARNKEV
IAGPDIISRGFVYVKENNDLMEECKSVVQNVLDECYTKNIYDWSTLKHNIRETLKKFLYQEIKRNPMILPVIMEV
>Mature_554_residues
AKKNIDKLKVIPLGGLNEIGKNMTVVEYKDDIIVVDCGMTFPDEEMLGVDIVIPDISYLEKNKEKIRGIVITHGHEDHIG
AIPYVLKKLDAPVYGTKLTMGLLENKILEHKLSLKSLHTVDYRKPIKLGAFSVEFVKVCHSIPDSASLAITTPVGVLFFT
GDFKIDYTPIDNNRMDFGRIASIGNKGVLALFADSTNVERQGYTLSEKSVGKTFINLFDDAPARIIVATFASNVHRIQQV
ISAAEHFNKKVALSGRSMINTVNVARELGYIKVGDKTIIDINDINKHKPNEIVLLTTGSQGEPMSAMTRMANGTHRKVHL
DPTDTVILSATPIPGNDNQVSSVINKLMEMGVKVIYSSLADVHVSGHACQEELKLMHSLVRPKFFVPLHGEMRHLKCHAN
LALDMGMKEKDIVIADNGNTIEFTRKSINLVDTKFASNILVDGLGVGDVGNVVLRDRRHLSEDGLIVVVITLDARNKEVI
AGPDIISRGFVYVKENNDLMEECKSVVQNVLDECYTKNIYDWSTLKHNIRETLKKFLYQEIKRNPMILPVIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61720; Mature: 61588

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNIDKLKVIPLGGLNEIGKNMTVVEYKDDIIVVDCGMTFPDEEMLGVDIVIPDISYL
CCCCCCCCEEEEECCCHHHHCCCCEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHH
EKNKEKIRGIVITHGHEDHIGAIPYVLKKLDAPVYGTKLTMGLLENKILEHKLSLKSLHT
HCCHHHEEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VDYRKPIKLGAFSVEFVKVCHSIPDSASLAITTPVGVLFFTGDFKIDYTPIDNNRMDFGR
CCCCCCCEECCCHHHHHHHHHCCCCCCCEEEECCCEEEEEECCEEEEEEECCCCCCCCHH
IASIGNKGVLALFADSTNVERQGYTLSEKSVGKTFINLFDDAPARIIVATFASNVHRIQQ
HHCCCCCCEEEEEECCCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
VISAAEHFNKKVALSGRSMINTVNVARELGYIKVGDKTIIDINDINKHKPNEIVLLTTGS
HHHHHHHCCCEEEECCCHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCCEEEEEEECC
QGEPMSAMTRMANGTHRKVHLDPTDTVILSATPIPGNDNQVSSVINKLMEMGVKVIYSSL
CCCCHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ADVHVSGHACQEELKLMHSLVRPKFFVPLHGEMRHLKCHANLALDMGMKEKDIVIADNGN
HHEEECCHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCEEEECCCCCCCEEEECCCC
TIEFTRKSINLVDTKFASNILVDGLGVGDVGNVVLRDRRHLSEDGLIVVVITLDARNKEV
EEEEECCCCCCHHHHHHHHHEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEE
IAGPDIISRGFVYVKENNDLMEECKSVVQNVLDECYTKNIYDWSTLKHNIRETLKKFLYQ
EECCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EIKRNPMILPVIMEV
HHHCCCEEEEEEECC
>Mature Secondary Structure 
AKKNIDKLKVIPLGGLNEIGKNMTVVEYKDDIIVVDCGMTFPDEEMLGVDIVIPDISYL
CCCCCCCEEEEECCCHHHHCCCCEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHH
EKNKEKIRGIVITHGHEDHIGAIPYVLKKLDAPVYGTKLTMGLLENKILEHKLSLKSLHT
HCCHHHEEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VDYRKPIKLGAFSVEFVKVCHSIPDSASLAITTPVGVLFFTGDFKIDYTPIDNNRMDFGR
CCCCCCCEECCCHHHHHHHHHCCCCCCCEEEECCCEEEEEECCEEEEEEECCCCCCCCHH
IASIGNKGVLALFADSTNVERQGYTLSEKSVGKTFINLFDDAPARIIVATFASNVHRIQQ
HHCCCCCCEEEEEECCCCCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
VISAAEHFNKKVALSGRSMINTVNVARELGYIKVGDKTIIDINDINKHKPNEIVLLTTGS
HHHHHHHCCCEEEECCCHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCCEEEEEEECC
QGEPMSAMTRMANGTHRKVHLDPTDTVILSATPIPGNDNQVSSVINKLMEMGVKVIYSSL
CCCCHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ADVHVSGHACQEELKLMHSLVRPKFFVPLHGEMRHLKCHANLALDMGMKEKDIVIADNGN
HHEEECCHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCEEEECCCCCCCEEEECCCC
TIEFTRKSINLVDTKFASNILVDGLGVGDVGNVVLRDRRHLSEDGLIVVVITLDARNKEV
EEEEECCCCCCHHHHHHHHHEECCCCCCCCCHHHHHHHHCCCCCCEEEEEEEECCCCCEE
IAGPDIISRGFVYVKENNDLMEECKSVVQNVLDECYTKNIYDWSTLKHNIRETLKKFLYQ
EECCCHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EIKRNPMILPVIMEV
HHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]