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The map label for this gene is spsK [H]

Identifier: 169823956

GI number: 169823956

Start: 307020

End: 307865

Strand: Direct

Name: spsK [H]

Synonym: FMG_0259

Alternate gene names: 169823956

Gene position: 307020-307865 (Clockwise)

Preceding gene: 169823955

Following gene: 169823957

Centisome position: 17.08

GC content: 34.52

Gene sequence:

>846_bases
ATGAGTAGAAATAGAATTTGGATAAGTGGAGCAGATGGAAAAATCGGAAAAGTATTGCAGAAATATTTGGATCCATTTGA
AGATGAGGTATTGGCAACAGACAAATACATCGTAGATATTGTAAATTCCGATGAAACTTCTATATTTGCAAGAAGAAACA
GACCGAATATAATCATCAATTGTTCTGCATTAACAGATCCTGTATTGTGTCAAGAAAATCCAGACGAGGCTTTCAGAGTA
AATGCTTTGGGTGCGAGAAACATGGCAGTTGCAGCGAACACTGTGGATGCAAAAATAATTCACATGTCTGCAGACGATGT
TTTCAGTGGACAATCCAGAAGTGCATACAGAGAATACGACACTCCACATCCTACGACAATGTATGGTAAAAGCAAATTGA
TGGGAGAAAATTTTGTAAGAGAATTCAGCGTGAAACATTTTATTTTGAGAACTAGCTGGTTATTTTCACCAGTAAATCAC
AGAGTAGAAGACATTATAAAAGAAGCAATTCAAACAGGAAAAGTTTACGTTCCTAAAGCACAATATTCTTCTCCAACAAG
TGCATATCAATTAGCTGAGTTTATCGTAAAATTGATGGCTACTTACGATTACGGTATTTATCATGCATCATGTGAAGGAG
TTGCTAGTAGGAAAGAATTTGCAGAAGAAATCTTGAAAATAAAAGGAATTGATGCAGAAGTAATCGAAGATGAAAATTTG
AGAATAGAAGGTCACAGACCAGATTTTACAGCTTTGGAAAACTTCTTGTTGAACACTTCACAAATTTACAAATTCACTGA
TTGGCAAACTTCTTTGCAAAAATATATCGAAAAAAACAATTTGTAA

Upstream 100 bases:

>100_bases
TGATTTGAAGGACAATCTTGTTGATGCGAAAATTCACCAAAGCATAATGGGATCAGACCATTGTCCAGTTGAATTGGAAA
TAAATTTTTAAGGAGAGATT

Downstream 100 bases:

>100_bases
TGCAAAATGAATTTTAAGAAAAAGGAAAGATATTTATGAAACAAAAGAAAAAAATGGGACTTACAACTCAAATTTTCATA
GGATTATTTTTAGGGTTGTT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSRNRIWISGADGKIGKVLQKYLDPFEDEVLATDKYIVDIVNSDETSIFARRNRPNIIINCSALTDPVLCQENPDEAFRV
NALGARNMAVAANTVDAKIIHMSADDVFSGQSRSAYREYDTPHPTTMYGKSKLMGENFVREFSVKHFILRTSWLFSPVNH
RVEDIIKEAIQTGKVYVPKAQYSSPTSAYQLAEFIVKLMATYDYGIYHASCEGVASRKEFAEEILKIKGIDAEVIEDENL
RIEGHRPDFTALENFLLNTSQIYKFTDWQTSLQKYIEKNNL

Sequences:

>Translated_281_residues
MSRNRIWISGADGKIGKVLQKYLDPFEDEVLATDKYIVDIVNSDETSIFARRNRPNIIINCSALTDPVLCQENPDEAFRV
NALGARNMAVAANTVDAKIIHMSADDVFSGQSRSAYREYDTPHPTTMYGKSKLMGENFVREFSVKHFILRTSWLFSPVNH
RVEDIIKEAIQTGKVYVPKAQYSSPTSAYQLAEFIVKLMATYDYGIYHASCEGVASRKEFAEEILKIKGIDAEVIEDENL
RIEGHRPDFTALENFLLNTSQIYKFTDWQTSLQKYIEKNNL
>Mature_280_residues
SRNRIWISGADGKIGKVLQKYLDPFEDEVLATDKYIVDIVNSDETSIFARRNRPNIIINCSALTDPVLCQENPDEAFRVN
ALGARNMAVAANTVDAKIIHMSADDVFSGQSRSAYREYDTPHPTTMYGKSKLMGENFVREFSVKHFILRTSWLFSPVNHR
VEDIIKEAIQTGKVYVPKAQYSSPTSAYQLAEFIVKLMATYDYGIYHASCEGVASRKEFAEEILKIKGIDAEVIEDENLR
IEGHRPDFTALENFLLNTSQIYKFTDWQTSLQKYIEKNNL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=161, Percent_Identity=30.4347826086957, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI33519455, Length=161, Percent_Identity=30.4347826086957, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1788352, Length=231, Percent_Identity=31.1688311688312, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 32029; Mature: 31897

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRNRIWISGADGKIGKVLQKYLDPFEDEVLATDKYIVDIVNSDETSIFARRNRPNIIIN
CCCCEEEEECCCCHHHHHHHHHHCCHHHHHEECCCEEEEEECCCCCEEEEECCCCCEEEE
CSALTDPVLCQENPDEAFRVNALGARNMAVAANTVDAKIIHMSADDVFSGQSRSAYREYD
ECCCCCCEECCCCCCCEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHCC
TPHPTTMYGKSKLMGENFVREFSVKHFILRTSWLFSPVNHRVEDIIKEAIQTGKVYVPKA
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECC
QYSSPTSAYQLAEFIVKLMATYDYGIYHASCEGVASRKEFAEEILKIKGIDAEVIEDENL
CCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCCE
RIEGHRPDFTALENFLLNTSQIYKFTDWQTSLQKYIEKNNL
EEECCCCCHHHHHHHHCCHHHHEEECCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRNRIWISGADGKIGKVLQKYLDPFEDEVLATDKYIVDIVNSDETSIFARRNRPNIIIN
CCCEEEEECCCCHHHHHHHHHHCCHHHHHEECCCEEEEEECCCCCEEEEECCCCCEEEE
CSALTDPVLCQENPDEAFRVNALGARNMAVAANTVDAKIIHMSADDVFSGQSRSAYREYD
ECCCCCCEECCCCCCCEEEEECCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHCC
TPHPTTMYGKSKLMGENFVREFSVKHFILRTSWLFSPVNHRVEDIIKEAIQTGKVYVPKA
CCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCEEEECC
QYSSPTSAYQLAEFIVKLMATYDYGIYHASCEGVASRKEFAEEILKIKGIDAEVIEDENL
CCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEECCCCE
RIEGHRPDFTALENFLLNTSQIYKFTDWQTSLQKYIEKNNL
EEECCCCCHHHHHHHHCCHHHHEEECCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]