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The map label for this gene is exoA [H]

Identifier: 169823955

GI number: 169823955

Start: 306249

End: 307010

Strand: Direct

Name: exoA [H]

Synonym: FMG_0258

Alternate gene names: 169823955

Gene position: 306249-307010 (Clockwise)

Preceding gene: 169823953

Following gene: 169823956

Centisome position: 17.04

GC content: 33.6

Gene sequence:

>762_bases
ATGAAATTAATATCATGGAATGTTAACGGATACAGAGCCGTTATGAAAAAAGGATTTATGGATGCTTTCAATAGTTTGAA
TGCGGATATTTTTTGTTTACAAGAAATCAAAATGAAAGAAAATCAAATGACTGACGAAATGAACATTGAAGGTTATCACA
GATATTCTTATTCAGCTAACAAAGCCGGTTATTCTGGAACTTTGGTTTACACAAAAGTTGAGCCTATTGGAGTAAGTTAT
GGGATTGGAATTGATGAACACGACACTGAAGGACGTGTGATTACTTGTGAATACGAAGATTTTTTCTTGGTGAATGTCTA
CACTCCAAACTCTAAACAAAAACTAGAACGCTTGGATTATAGAATGGTGTGGGAAGATGAATTCCACAATTATTTGAATA
TTTTAAGAGATAGAAAACCTGTAATTGTGTGTGGAGATTTGAATGTCGCTCACAATGAAATTGATTTGAAAAATCCACAA
TCAAACAGACGCTCAGCAGGATTCACCGATGAAGAAAGAAACAAAATGTCACTTTTATTAAATGATGGATACATCGATAC
ATTCAGATATTTTTATCCAGATAAAACTGACGAATATTCGTATTGGTCGTATTTTGCAAAGGCGAGAGAAAGAAACGCTG
GTTGGAGAATAGATTATTTCTTGGTTAGCGATGATTTGAAGGACAATCTTGTTGATGCGAAAATTCACCAAAGCATAATG
GGATCAGACCATTGTCCAGTTGAATTGGAAATAAATTTTTAA

Upstream 100 bases:

>100_bases
ATTTTCGTTTATTTGGGTATAAATAATTGTTAGAAAAATTTACAAATATAAGTCACAAAACTGTATATGATATAATAAAT
AACAAATAAGAGGTGAGTAT

Downstream 100 bases:

>100_bases
GGAGAGATTATGAGTAGAAATAGAATTTGGATAAGTGGAGCAGATGGAAAAATCGGAAAAGTATTGCAGAAATATTTGGA
TCCATTTGAAGATGAGGTAT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKLISWNVNGYRAVMKKGFMDAFNSLNADIFCLQEIKMKENQMTDEMNIEGYHRYSYSANKAGYSGTLVYTKVEPIGVSY
GIGIDEHDTEGRVITCEYEDFFLVNVYTPNSKQKLERLDYRMVWEDEFHNYLNILRDRKPVIVCGDLNVAHNEIDLKNPQ
SNRRSAGFTDEERNKMSLLLNDGYIDTFRYFYPDKTDEYSYWSYFAKARERNAGWRIDYFLVSDDLKDNLVDAKIHQSIM
GSDHCPVELEINF

Sequences:

>Translated_253_residues
MKLISWNVNGYRAVMKKGFMDAFNSLNADIFCLQEIKMKENQMTDEMNIEGYHRYSYSANKAGYSGTLVYTKVEPIGVSY
GIGIDEHDTEGRVITCEYEDFFLVNVYTPNSKQKLERLDYRMVWEDEFHNYLNILRDRKPVIVCGDLNVAHNEIDLKNPQ
SNRRSAGFTDEERNKMSLLLNDGYIDTFRYFYPDKTDEYSYWSYFAKARERNAGWRIDYFLVSDDLKDNLVDAKIHQSIM
GSDHCPVELEINF
>Mature_253_residues
MKLISWNVNGYRAVMKKGFMDAFNSLNADIFCLQEIKMKENQMTDEMNIEGYHRYSYSANKAGYSGTLVYTKVEPIGVSY
GIGIDEHDTEGRVITCEYEDFFLVNVYTPNSKQKLERLDYRMVWEDEFHNYLNILRDRKPVIVCGDLNVAHNEIDLKNPQ
SNRRSAGFTDEERNKMSLLLNDGYIDTFRYFYPDKTDEYSYWSYFAKARERNAGWRIDYFLVSDDLKDNLVDAKIHQSIM
GSDHCPVELEINF

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=253, Percent_Identity=46.6403162055336, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI18375503, Length=253, Percent_Identity=46.6403162055336, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI18375501, Length=253, Percent_Identity=46.6403162055336, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI18375507, Length=312, Percent_Identity=29.8076923076923, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71989536, Length=255, Percent_Identity=41.9607843137255, Blast_Score=207, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI71989539, Length=143, Percent_Identity=33.5664335664336, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI221330655, Length=254, Percent_Identity=48.0314960629921, Blast_Score=265, Evalue=2e-71,
Organism=Drosophila melanogaster, GI17136678, Length=250, Percent_Identity=48.4, Blast_Score=265, Evalue=3e-71,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29742; Mature: 29742

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLISWNVNGYRAVMKKGFMDAFNSLNADIFCLQEIKMKENQMTDEMNIEGYHRYSYSAN
CEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCEEEEEECCCC
KAGYSGTLVYTKVEPIGVSYGIGIDEHDTEGRVITCEYEDFFLVNVYTPNSKQKLERLDY
CCCCCEEEEEEEECCCCEEEECCCCCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHCCE
RMVWEDEFHNYLNILRDRKPVIVCGDLNVAHNEIDLKNPQSNRRSAGFTDEERNKMSLLL
EEEEHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEE
NDGYIDTFRYFYPDKTDEYSYWSYFAKARERNAGWRIDYFLVSDDLKDNLVDAKIHQSIM
ECCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
GSDHCPVELEINF
CCCCCCEEEEECC
>Mature Secondary Structure
MKLISWNVNGYRAVMKKGFMDAFNSLNADIFCLQEIKMKENQMTDEMNIEGYHRYSYSAN
CEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCEEEEEECCCC
KAGYSGTLVYTKVEPIGVSYGIGIDEHDTEGRVITCEYEDFFLVNVYTPNSKQKLERLDY
CCCCCEEEEEEEECCCCEEEECCCCCCCCCCCEEEEECCCEEEEEEECCCHHHHHHHCCE
RMVWEDEFHNYLNILRDRKPVIVCGDLNVAHNEIDLKNPQSNRRSAGFTDEERNKMSLLL
EEEEHHHHHHHHHHHHCCCCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEE
NDGYIDTFRYFYPDKTDEYSYWSYFAKARERNAGWRIDYFLVSDDLKDNLVDAKIHQSIM
ECCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
GSDHCPVELEINF
CCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]