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The map label for this gene is dnaN [H]

Identifier: 169823699

GI number: 169823699

Start: 2110

End: 3213

Strand: Direct

Name: dnaN [H]

Synonym: FMG_0002

Alternate gene names: 169823699

Gene position: 2110-3213 (Clockwise)

Preceding gene: 169823698

Following gene: 169823700

Centisome position: 0.12

GC content: 34.96

Gene sequence:

>1104_bases
ATGAAATTAAAAATATCTCAAAAAGAATTACTAAAACATATCAACATTGCCCAAAAAGCGGTGTCATCTAGGACAACTAT
TCAGATATTAGAGGGAATTTTGTTCATTGCAGAAGAAAACTGTCTACGCCTTGTGGCGACTGATTTGGAACTTATCGTGG
ATACGAGAGCAAATTGTGAAATCTACGAAAAAGGACAAATTGTAATCAACTCGTCAATGATAGGTAATATAATAAGAAAA
CTTCCAGACGCCGATATATATATAGAAGTTGACGATAATAACATCAATATCAAATGTTTGTCTACGGAATTTAACATTCA
AGGTCAAGATTCCAACGAATTTCCATCTCTTCCTTATATAGATAAGGATGTTAAATTGACTTTGGAACAATCTGAACTTA
GAGATTCTATCAGAAAAACATCATTTGCTACGAGTATGGACCAAACAAGGCCGGATTTGACTGGTGTTTTGTTCAATATT
ATGAATGATAGAATCGAGTTTGTGGCATTGGATGGTTACAGAGTTTCACTTGATTGTTTGAGTAATGTGAGTGAATTCGA
AATGAAGGCCATTGTTCCTGCGAGAGCTTTGAATGAAGTCTACAAGATTTTGGAAGAAGGGGAAATCACTCTTAACATTA
TTACTGGAAATATTATTTTCAAATTGGAAAACACTGATGTTTATTCTAGACTAATCGATGGTCAATACATCGACTACAAT
CAAGTTATGAAACAAGATTTCTCAACTGTTGTTACTATAGAAAAGAGAGCTTTGCAAATGGCATTGGAAAGAGCGTCTTT
GATGGCAAAAGAAGACAAGGCAAACTTGGTTAAGCTTGATTTCTCGGGAAATGAATTGCACATTTCATCTAATACTGATA
TTGGTAAGGTGGATGAGTATGTGAAGTGCGAAATCGGTGGAGAGGATTTGAGGATAGCTTTTAATGCGAAGTATTTGTTG
GATGGGTTGAAGGTAATAGATGATGACGATATTACATTGAATATGAGTGGAAGTTTGAGACCGATGGTTTTCAAGCCACT
TAACGATACGAATTACACTTATCTTGTCCTTCCAGTAAAACTTGCAGGAGGTAATAATGATTAG

Upstream 100 bases:

>100_bases
ACCACAAATTACAAACAAACTTGAGTTATCCACAATACAAACAGCCCTACTACTACTATTACTAACTATTATTATTATTT
GTATAAACGAAAGGTAAAAT

Downstream 100 bases:

>100_bases
TGTCAAAATAGAATCTGAGTTTATTAAATTGGAACAGTTTCTAAAATTCGCATCCATCGCACAAACTGGTGGCATGGCGA
AAGAACTCATAAAAGAATCG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKLKISQKELLKHINIAQKAVSSRTTIQILEGILFIAEENCLRLVATDLELIVDTRANCEIYEKGQIVINSSMIGNIIRK
LPDADIYIEVDDNNINIKCLSTEFNIQGQDSNEFPSLPYIDKDVKLTLEQSELRDSIRKTSFATSMDQTRPDLTGVLFNI
MNDRIEFVALDGYRVSLDCLSNVSEFEMKAIVPARALNEVYKILEEGEITLNIITGNIIFKLENTDVYSRLIDGQYIDYN
QVMKQDFSTVVTIEKRALQMALERASLMAKEDKANLVKLDFSGNELHISSNTDIGKVDEYVKCEIGGEDLRIAFNAKYLL
DGLKVIDDDDITLNMSGSLRPMVFKPLNDTNYTYLVLPVKLAGGNND

Sequences:

>Translated_367_residues
MKLKISQKELLKHINIAQKAVSSRTTIQILEGILFIAEENCLRLVATDLELIVDTRANCEIYEKGQIVINSSMIGNIIRK
LPDADIYIEVDDNNINIKCLSTEFNIQGQDSNEFPSLPYIDKDVKLTLEQSELRDSIRKTSFATSMDQTRPDLTGVLFNI
MNDRIEFVALDGYRVSLDCLSNVSEFEMKAIVPARALNEVYKILEEGEITLNIITGNIIFKLENTDVYSRLIDGQYIDYN
QVMKQDFSTVVTIEKRALQMALERASLMAKEDKANLVKLDFSGNELHISSNTDIGKVDEYVKCEIGGEDLRIAFNAKYLL
DGLKVIDDDDITLNMSGSLRPMVFKPLNDTNYTYLVLPVKLAGGNND
>Mature_367_residues
MKLKISQKELLKHINIAQKAVSSRTTIQILEGILFIAEENCLRLVATDLELIVDTRANCEIYEKGQIVINSSMIGNIIRK
LPDADIYIEVDDNNINIKCLSTEFNIQGQDSNEFPSLPYIDKDVKLTLEQSELRDSIRKTSFATSMDQTRPDLTGVLFNI
MNDRIEFVALDGYRVSLDCLSNVSEFEMKAIVPARALNEVYKILEEGEITLNIITGNIIFKLENTDVYSRLIDGQYIDYN
QVMKQDFSTVVTIEKRALQMALERASLMAKEDKANLVKLDFSGNELHISSNTDIGKVDEYVKCEIGGEDLRIAFNAKYLL
DGLKVIDDDDITLNMSGSLRPMVFKPLNDTNYTYLVLPVKLAGGNND

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=25.0681198910082, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41418; Mature: 41418

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKISQKELLKHINIAQKAVSSRTTIQILEGILFIAEENCLRLVATDLELIVDTRANCE
CCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCEEEEECCEEEEEECCCCCE
IYEKGQIVINSSMIGNIIRKLPDADIYIEVDDNNINIKCLSTEFNIQGQDSNEFPSLPYI
EEECCEEEEECHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEEEEEECCCCCCCCCCCCC
DKDVKLTLEQSELRDSIRKTSFATSMDQTRPDLTGVLFNIMNDRIEFVALDGYRVSLDCL
CCCEEEEEEHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHCCCEEEEEECCEEEEHHHH
SNVSEFEMKAIVPARALNEVYKILEEGEITLNIITGNIIFKLENTDVYSRLIDGQYIDYN
CCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCHHHHHHHCCCCCCHH
QVMKQDFSTVVTIEKRALQMALERASLMAKEDKANLVKLDFSGNELHISSNTDIGKVDEY
HHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCCCE
VKCEIGGEDLRIAFNAKYLLDGLKVIDDDDITLNMSGSLRPMVFKPLNDTNYTYLVLPVK
EEEEECCCEEEEEEECHHHHCCCEEECCCCEEEEECCCCCCEEEECCCCCCEEEEEEEEE
LAGGNND
ECCCCCC
>Mature Secondary Structure
MKLKISQKELLKHINIAQKAVSSRTTIQILEGILFIAEENCLRLVATDLELIVDTRANCE
CCEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCEEEEECCEEEEEECCCCCE
IYEKGQIVINSSMIGNIIRKLPDADIYIEVDDNNINIKCLSTEFNIQGQDSNEFPSLPYI
EEECCEEEEECHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEEEEEECCCCCCCCCCCCC
DKDVKLTLEQSELRDSIRKTSFATSMDQTRPDLTGVLFNIMNDRIEFVALDGYRVSLDCL
CCCEEEEEEHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHCCCEEEEEECCEEEEHHHH
SNVSEFEMKAIVPARALNEVYKILEEGEITLNIITGNIIFKLENTDVYSRLIDGQYIDYN
CCCHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEEEEEEEEECCHHHHHHHCCCCCCHH
QVMKQDFSTVVTIEKRALQMALERASLMAKEDKANLVKLDFSGNELHISSNTDIGKVDEY
HHHHHCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCCCCCCCCE
VKCEIGGEDLRIAFNAKYLLDGLKVIDDDDITLNMSGSLRPMVFKPLNDTNYTYLVLPVK
EEEEECCCEEEEEEECHHHHCCCEEECCCCEEEEECCCCCCEEEECCCCCCEEEEEEEEE
LAGGNND
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10427704; 11058132 [H]