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The map label for this gene is mdeA [H]

Identifier: 169630766

GI number: 169630766

Start: 3742212

End: 3743573

Strand: Direct

Name: mdeA [H]

Synonym: MAB_3687

Alternate gene names: 169630766

Gene position: 3742212-3743573 (Clockwise)

Preceding gene: 169630764

Following gene: 169630767

Centisome position: 73.85

GC content: 65.27

Gene sequence:

>1362_bases
ATGACCGATATCGATCCCACCACAGCAGACAGTCGCTCGGACAAGCCTCGCTCCGCCCAGTGGGCGTTCGAGACCAAGCA
GATCCACGCGGGCCAGTCCGCCGACGCGGCAACCAACGCGCGGGCCCTGCCGATCTACCAGACCACCTCCTACGTCTTCA
ACGACACCGCGCACGCCGCGGCGCTCTTCGGGCTGTCCGAAGAGGGCAACATCTACACCCGCATCGGCAACCCCACCCAG
GATGTCGTCGAGCAGCGCATCGCAGCCCTGGAAGGTGGCGTGGCCGCGCTGCTACTTGCCTCCGGACAGGCGGCGGCGAC
CCTGGCGATCGTGAACATCGCACAGGCCGGCGACAACATCGTCTCCAGCCCGCGCCTGTACGGAGGCACCTACAATCTGC
TGCACTACACGCTGCCACGACTGGGCATCTCGACTACCTTCGTCGAAGACCCCGACGATCTGGAGTCGTGGCGCAACGCC
ATCACCCCGGATACCAAGGCGCTGTTCGCCGAGTCGATCTCCAACCCGCAGAACGACATTCTCGACATCGCCGGCCTGTC
GGAGATCGCGCACGCGGCAGGCATCCCGCTGATCGTCGACAACACCGTCGCCACCCCCTATCTGATCCAGCCGCTGGCAC
ACGGGGCCGACGTCGTGGTGCATTCGGCCACCAAATACCTTGGTGGACACGGCAATGCAATCGGCGGCGTGATCATCGAC
GGAGGTACCTTCGACTGGACCCAGGGTCGCCATCCCGGATTCACCACCCCCGACCCGAGTTACCACGGCGTGGTGTTCGC
CGATCTGGGCGCGCCGGCCTACGCACTTAAGGCGCGTGTGCAGCTGCTGCGCGACCTCGGTCCCGCCATCTCGCCATTCA
ACGCATTCCTCATCGCTCAGGGTTTGGAAACATTGAGCCTGCGCGTGGAGCGGCACGTGGCGAACGCACAGCGGGTGGCC
GAATTCCTTGTCGCACAGCCGGAAGTCACTCGCGTCAACTACGCCGGGCTACCCACATCTCCATGGTACGAGCGCGCGAA
GGCCTTGGCCCCCAAGGGAGCCGGTGCCGTGCTCGCATTCGAACTGGACGGTGGTGTCGAGGCTGCGACCACCTTCATCG
ATTCCCTGACCCTGCACAGCCAGGTCGCCAACATCGGTGATGTGCGTTCGCTTGTCATCCACCCGGCCACCACAACGCAC
GGACAGCTCTCCCCTGCCGAACAGGTGGCCTCCGGGGTCACGCCGGGACTGGTCCGGTTGGCCGTCGGCATCGAGGGCAT
CGACGACATCCTGGCCGACCTGGAGTCCGGATTCACGGCCGTACGCAGGCTCAAACAACCCGCGGCGGTGGCATCATCGT
GA

Upstream 100 bases:

>100_bases
TGTTGTCGGTTCCGGTTGTTCTTCGCGCCAGCGGCTCATCAGCACTGTCGCTGTTGCCCCCAGCCCTGACTGCCCTACCC
GACTACCCCAGGAGAGCGCC

Downstream 100 bases:

>100_bases
CCATCGAACAGCAGCTTCCGGTCCCCCACATCGCCCTACCCCAGGGCGATGAGATCGCCTATGTACCAATAGGTTCGATC
ACTCTGGAAAGCGGTGCTGT

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MTDIDPTTADSRSDKPRSAQWAFETKQIHAGQSADAATNARALPIYQTTSYVFNDTAHAAALFGLSEEGNIYTRIGNPTQ
DVVEQRIAALEGGVAALLLASGQAAATLAIVNIAQAGDNIVSSPRLYGGTYNLLHYTLPRLGISTTFVEDPDDLESWRNA
ITPDTKALFAESISNPQNDILDIAGLSEIAHAAGIPLIVDNTVATPYLIQPLAHGADVVVHSATKYLGGHGNAIGGVIID
GGTFDWTQGRHPGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGPAISPFNAFLIAQGLETLSLRVERHVANAQRVA
EFLVAQPEVTRVNYAGLPTSPWYERAKALAPKGAGAVLAFELDGGVEAATTFIDSLTLHSQVANIGDVRSLVIHPATTTH
GQLSPAEQVASGVTPGLVRLAVGIEGIDDILADLESGFTAVRRLKQPAAVASS

Sequences:

>Translated_453_residues
MTDIDPTTADSRSDKPRSAQWAFETKQIHAGQSADAATNARALPIYQTTSYVFNDTAHAAALFGLSEEGNIYTRIGNPTQ
DVVEQRIAALEGGVAALLLASGQAAATLAIVNIAQAGDNIVSSPRLYGGTYNLLHYTLPRLGISTTFVEDPDDLESWRNA
ITPDTKALFAESISNPQNDILDIAGLSEIAHAAGIPLIVDNTVATPYLIQPLAHGADVVVHSATKYLGGHGNAIGGVIID
GGTFDWTQGRHPGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGPAISPFNAFLIAQGLETLSLRVERHVANAQRVA
EFLVAQPEVTRVNYAGLPTSPWYERAKALAPKGAGAVLAFELDGGVEAATTFIDSLTLHSQVANIGDVRSLVIHPATTTH
GQLSPAEQVASGVTPGLVRLAVGIEGIDDILADLESGFTAVRRLKQPAAVASS
>Mature_452_residues
TDIDPTTADSRSDKPRSAQWAFETKQIHAGQSADAATNARALPIYQTTSYVFNDTAHAAALFGLSEEGNIYTRIGNPTQD
VVEQRIAALEGGVAALLLASGQAAATLAIVNIAQAGDNIVSSPRLYGGTYNLLHYTLPRLGISTTFVEDPDDLESWRNAI
TPDTKALFAESISNPQNDILDIAGLSEIAHAAGIPLIVDNTVATPYLIQPLAHGADVVVHSATKYLGGHGNAIGGVIIDG
GTFDWTQGRHPGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGPAISPFNAFLIAQGLETLSLRVERHVANAQRVAE
FLVAQPEVTRVNYAGLPTSPWYERAKALAPKGAGAVLAFELDGGVEAATTFIDSLTLHSQVANIGDVRSLVIHPATTTHG
QLSPAEQVASGVTPGLVRLAVGIEGIDDILADLESGFTAVRRLKQPAAVASS

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=403, Percent_Identity=32.258064516129, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI299473759, Length=403, Percent_Identity=28.7841191066998, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI25453487, Length=402, Percent_Identity=28.1094527363184, Blast_Score=153, Evalue=3e-37,
Organism=Escherichia coli, GI1790375, Length=420, Percent_Identity=33.5714285714286, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1789383, Length=425, Percent_Identity=25.6470588235294, Blast_Score=135, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71980708, Length=401, Percent_Identity=34.4139650872818, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17538055, Length=407, Percent_Identity=32.4324324324324, Blast_Score=198, Evalue=5e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=408, Percent_Identity=31.8627450980392, Blast_Score=188, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=441, Percent_Identity=47.1655328798186, Blast_Score=350, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=33.806146572104, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=436, Percent_Identity=26.1467889908257, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=224, Percent_Identity=29.0178571428571, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=431, Percent_Identity=25.0580046403712, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922554, Length=419, Percent_Identity=33.1742243436754, Blast_Score=212, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47600; Mature: 47469

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIDPTTADSRSDKPRSAQWAFETKQIHAGQSADAATNARALPIYQTTSYVFNDTAHAA
CCCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCEEEEEECCHHHCCCCHHHH
ALFGLSEEGNIYTRIGNPTQDVVEQRIAALEGGVAALLLASGQAAATLAIVNIAQAGDNI
HHEECCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHEEEEEEHHHCCCCH
VSSPRLYGGTYNLLHYTLPRLGISTTFVEDPDDLESWRNAITPDTKALFAESISNPQNDI
HCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
LDIAGLSEIAHAAGIPLIVDNTVATPYLIQPLAHGADVVVHSATKYLGGHGNAIGGVIID
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCEEEEHHHHHHCCCCCEECEEEEC
GGTFDWTQGRHPGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGPAISPFNAFLIAQ
CCEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GLETLSLRVERHVANAQRVAEFLVAQPEVTRVNYAGLPTSPWYERAKALAPKGAGAVLAF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEE
ELDGGVEAATTFIDSLTLHSQVANIGDVRSLVIHPATTTHGQLSPAEQVASGVTPGLVRL
EECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHH
AVGIEGIDDILADLESGFTAVRRLKQPAAVASS
HHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
TDIDPTTADSRSDKPRSAQWAFETKQIHAGQSADAATNARALPIYQTTSYVFNDTAHAA
CCCCCCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCCCCEEEEEECCHHHCCCCHHHH
ALFGLSEEGNIYTRIGNPTQDVVEQRIAALEGGVAALLLASGQAAATLAIVNIAQAGDNI
HHEECCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHEEEEEEHHHCCCCH
VSSPRLYGGTYNLLHYTLPRLGISTTFVEDPDDLESWRNAITPDTKALFAESISNPQNDI
HCCCCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
LDIAGLSEIAHAAGIPLIVDNTVATPYLIQPLAHGADVVVHSATKYLGGHGNAIGGVIID
HHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHCCCCEEEEHHHHHHCCCCCEECEEEEC
GGTFDWTQGRHPGFTTPDPSYHGVVFADLGAPAYALKARVQLLRDLGPAISPFNAFLIAQ
CCEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
GLETLSLRVERHVANAQRVAEFLVAQPEVTRVNYAGLPTSPWYERAKALAPKGAGAVLAF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEE
ELDGGVEAATTFIDSLTLHSQVANIGDVRSLVIHPATTTHGQLSPAEQVASGVTPGLVRL
EECCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHHH
AVGIEGIDDILADLESGFTAVRRLKQPAAVASS
HHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]