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The map label for this gene is leuA2 [H]

Identifier: 169630634

GI number: 169630634

Start: 3598294

End: 3599553

Strand: Direct

Name: leuA2 [H]

Synonym: MAB_3553

Alternate gene names: 169630634

Gene position: 3598294-3599553 (Clockwise)

Preceding gene: 169630633

Following gene: 169630635

Centisome position: 71.01

GC content: 57.62

Gene sequence:

>1260_bases
ATGCTCACTTTTCCCCAAGGACATACCCCCCGTTATCGCGACTTCACCATCGACGCAATCTCTGACGAGACGATGCGCGA
GGGTGGTGAACGCGCCCCCTTCGGCGCTACGGACGATCAGAAGATCCAGCTGATTCGGGCCATTACCGACGCGGGTATCA
CCGACATCGACGTGGGCTCAGGGATCACCGAGGCATCATTTGTCCGCGCCTTGTTGGACGCCCAGCAGCTCGAAAGCACC
ATCGCCCCCGACGCGACGTTCTCCTTCAATCTCACCCTCAAGACCTGGGAACCCCTGTGCGAACAGCTCGAACGAGAGCT
GCCCAGCAGTTACCTGTCCGATATCTATGTGAGCATCGGAATGATCGAAATCGATTCAGAAAACCGGCTTTTTGAGCGCG
TGGCCGAGAGACTGCGGTCAATTGGGGTCGGAAAGCTGCGGTCGAGTCTCCTCAATGCGTTCAGCATCACGGTCGACGAG
CAGAAGTACGAGCACCTCAACACACAAATCGAGCGATGCCGCCAACAGGGCATCTCGCTTATCCGCATCAACGACTCGGT
CGGCACCCTGGTGCCCGATTCCACAGCAGTGCTGGCCGCCAATCTTGTCCGGGATAACCCGGATATCACCTTCTATCTGC
ACGGACACAACGACCGGGGACTAGGCACCGCGAATTCTTTGACGTCGGTGTTCCACGGTTTCCAGATGATTGAGGGCGGC
GTCGCCGGGGTCGGCAATCGTTCCGGACTGGCCGAAATCGAGTGTATTGCAGCAATATTCGCAGAAAACAACATTCGCGT
CGCGGCAGGCACGCCGGATCTTGACGGGCTGTGCCGCGCGGCACGGTTGTCCGAGGATGTTTTCCTCACCGCCCCCGATC
CCTTCAGAGCGGTGAGCGGCTTTCTAACGGAGAACGAGAATGCCGGGATCGTGAATGTTCCTGATTACCTTGGCGTTTCC
CGTTCGGTCAAATACTTCCTGAACAGGATCGGCCTCTTTCCGACGTATGTCCGCCAGATACTGTCAGAAAGCGAATTTGA
TTCCAGCACCGTCGATGACGACTCGTTCATCGACCAGGTATATGAACGACTGGCCGCACATATGGATGCGTTGCACCTGC
GTAAACGAACCGAATTCGCCCACCTGTCACGGGCCATCCAAGATTTCTACACCGAGATGGTCCGTCTCGATGACGTCCGA
GACTGCGCATTGGAGGTGTTGCGAGATCGTGGGAGCGCGCACGCGAAGGTCGCGTCGTGA

Upstream 100 bases:

>100_bases
TATAACCACCCCACTTAGCGTTCGCCGTAGCGAACGGTCGTATTCATTGCCGTCAAAGTTGACGGGCAGTATCCCCAACA
CGTCAGTGCCGAGGAATGCC

Downstream 100 bases:

>100_bases
GAGTGCTGGTATTCGGCGCGGGGGGCGTCGGTCTGTATTTCTCGGCCGTTCTCGCGCAAGCCGGTCACCACGTGACCATT
GTTGGCCGGCCGGCCACCGT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MLTFPQGHTPRYRDFTIDAISDETMREGGERAPFGATDDQKIQLIRAITDAGITDIDVGSGITEASFVRALLDAQQLEST
IAPDATFSFNLTLKTWEPLCEQLERELPSSYLSDIYVSIGMIEIDSENRLFERVAERLRSIGVGKLRSSLLNAFSITVDE
QKYEHLNTQIERCRQQGISLIRINDSVGTLVPDSTAVLAANLVRDNPDITFYLHGHNDRGLGTANSLTSVFHGFQMIEGG
VAGVGNRSGLAEIECIAAIFAENNIRVAAGTPDLDGLCRAARLSEDVFLTAPDPFRAVSGFLTENENAGIVNVPDYLGVS
RSVKYFLNRIGLFPTYVRQILSESEFDSSTVDDDSFIDQVYERLAAHMDALHLRKRTEFAHLSRAIQDFYTEMVRLDDVR
DCALEVLRDRGSAHAKVAS

Sequences:

>Translated_419_residues
MLTFPQGHTPRYRDFTIDAISDETMREGGERAPFGATDDQKIQLIRAITDAGITDIDVGSGITEASFVRALLDAQQLEST
IAPDATFSFNLTLKTWEPLCEQLERELPSSYLSDIYVSIGMIEIDSENRLFERVAERLRSIGVGKLRSSLLNAFSITVDE
QKYEHLNTQIERCRQQGISLIRINDSVGTLVPDSTAVLAANLVRDNPDITFYLHGHNDRGLGTANSLTSVFHGFQMIEGG
VAGVGNRSGLAEIECIAAIFAENNIRVAAGTPDLDGLCRAARLSEDVFLTAPDPFRAVSGFLTENENAGIVNVPDYLGVS
RSVKYFLNRIGLFPTYVRQILSESEFDSSTVDDDSFIDQVYERLAAHMDALHLRKRTEFAHLSRAIQDFYTEMVRLDDVR
DCALEVLRDRGSAHAKVAS
>Mature_419_residues
MLTFPQGHTPRYRDFTIDAISDETMREGGERAPFGATDDQKIQLIRAITDAGITDIDVGSGITEASFVRALLDAQQLEST
IAPDATFSFNLTLKTWEPLCEQLERELPSSYLSDIYVSIGMIEIDSENRLFERVAERLRSIGVGKLRSSLLNAFSITVDE
QKYEHLNTQIERCRQQGISLIRINDSVGTLVPDSTAVLAANLVRDNPDITFYLHGHNDRGLGTANSLTSVFHGFQMIEGG
VAGVGNRSGLAEIECIAAIFAENNIRVAAGTPDLDGLCRAARLSEDVFLTAPDPFRAVSGFLTENENAGIVNVPDYLGVS
RSVKYFLNRIGLFPTYVRQILSESEFDSSTVDDDSFIDQVYERLAAHMDALHLRKRTEFAHLSRAIQDFYTEMVRLDDVR
DCALEVLRDRGSAHAKVAS

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011008
- InterPro:   IPR011872
- InterPro:   IPR000891
- InterPro:   IPR019887 [H]

Pfam domain/function: PF01037 AsnC_trans_reg; PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 46409; Mature: 46409

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFPQGHTPRYRDFTIDAISDETMREGGERAPFGATDDQKIQLIRAITDAGITDIDVGS
CCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCC
GITEASFVRALLDAQQLESTIAPDATFSFNLTLKTWEPLCEQLERELPSSYLSDIYVSIG
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHEE
MIEIDSENRLFERVAERLRSIGVGKLRSSLLNAFSITVDEQKYEHLNTQIERCRQQGISL
EEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHCCCEE
IRINDSVGTLVPDSTAVLAANLVRDNPDITFYLHGHNDRGLGTANSLTSVFHGFQMIEGG
EEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
VAGVGNRSGLAEIECIAAIFAENNIRVAAGTPDLDGLCRAARLSEDVFLTAPDPFRAVSG
CCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHHH
FLTENENAGIVNVPDYLGVSRSVKYFLNRIGLFPTYVRQILSESEFDSSTVDDDSFIDQV
HHCCCCCCCEEECCHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHH
YERLAAHMDALHLRKRTEFAHLSRAIQDFYTEMVRLDDVRDCALEVLRDRGSAHAKVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLTFPQGHTPRYRDFTIDAISDETMREGGERAPFGATDDQKIQLIRAITDAGITDIDVGS
CCCCCCCCCCCCCCEEEECCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEECCC
GITEASFVRALLDAQQLESTIAPDATFSFNLTLKTWEPLCEQLERELPSSYLSDIYVSIG
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHEE
MIEIDSENRLFERVAERLRSIGVGKLRSSLLNAFSITVDEQKYEHLNTQIERCRQQGISL
EEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHCCCEE
IRINDSVGTLVPDSTAVLAANLVRDNPDITFYLHGHNDRGLGTANSLTSVFHGFQMIEGG
EEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
VAGVGNRSGLAEIECIAAIFAENNIRVAAGTPDLDGLCRAARLSEDVFLTAPDPFRAVSG
CCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCEEEECCCHHHHHHH
FLTENENAGIVNVPDYLGVSRSVKYFLNRIGLFPTYVRQILSESEFDSSTVDDDSFIDQV
HHCCCCCCCEEECCHHHCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHHHHHH
YERLAAHMDALHLRKRTEFAHLSRAIQDFYTEMVRLDDVRDCALEVLRDRGSAHAKVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479 [H]