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The map label for this gene is paaF [C]

Identifier: 169630431

GI number: 169630431

Start: 3398099

End: 3399019

Strand: Direct

Name: paaF [C]

Synonym: MAB_3350

Alternate gene names: 169630431

Gene position: 3398099-3399019 (Clockwise)

Preceding gene: 169630430

Following gene: 169630435

Centisome position: 67.06

GC content: 65.8

Gene sequence:

>921_bases
ATGGCTGATTCTCTTCACGCAAGCGGCTCATCGGTGTCTGATTCTCTTCGCGCAAGCGGCTCATCGGTGTCTGATTCTCT
TCACGCAAGCGGCTCATCGGCCGACGGCTGGAAAGCCTTCACCGTCGAGACCACCGGGCATATCGCACAGGTCACCCTCA
TCGGCCCCGGCAAGGGCAATGCGATGGGCCCCGACTTTTGGCGAGAGCTACCGCTGATCTTCACCGAGCTCGACGCCGAC
CCCGAGGTACGGGCGGTGGTGCTCGCGGCCGCCGGTCGGCACTTCAGCTACGGATTGGACCTGCCGGCGATGGCGGGCAC
CTTCATGCCGCTTATGGCCGAGAAGGCACTGGCCAAACCGCGCACAGACTTCCTCGACGAGATCCGCCGGCTACAAGCCT
CAGTGACAGCCGTCGCCGACTGCCGCAAGCCGGTGATCGCGGCAATTCAAGGCTGGTGCATCGGTGGCGGGGTGGATCTC
ATCGCCGCCGCCGATATCCGGTACGCCAGCTCCGAGGCAAAGTTCAGCGTGCGCGAGGCCAAGGTGGCGATTGTGGCCGA
TATCGGTTCGCTGCACCGGCTTCCGCCGATCATCGGTGACGGCCACCTGCGCGAACTGGCGTTCACCGGCAAGGACATCG
ACGCTGCCCGGGCCGAGAAGATCGGTCTCGTCAACGACGTGTTCGACACCCCCGAAGCGACGCTGGCCGCCGCGCATGCC
ACCGCGGCCGAGATCGCGGCGAATCCCCCGCTGGTGGTGCAGGGCGTCAAGGACGTCCTGGGGCGCGAGCGCGAGAACGT
CGTGGCCGACGGCCTCAAGTACGTCTCCACCTGGAATGCCGCGTTCCTGCCGTCGGAGGATCTACAGGAGGCCATCGGCG
CGGTCTTCGAGAAGCGGGAACCCGACTTCAAGGGGCGCTAG

Upstream 100 bases:

>100_bases
CGATGTCCAGTCGTGGGCGATGTCGTGGCCTTCGGTCATCAAACAGCTTCGCGAAGACCGCAAGGGCCTGCTGCCCGCGT
GAACCCGTAGGCTCACGCCC

Downstream 100 bases:

>100_bases
GCGCGGTAGCGGCCCCAGTGCACGGCATCCTCCAGGGGGCGCCCTGTGCGCCAGCCAAACCGCTCCAGGTCGGGCCGTTC
GGCGAATTCACGTACCGGAC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MADSLHASGSSVSDSLRASGSSVSDSLHASGSSADGWKAFTVETTGHIAQVTLIGPGKGNAMGPDFWRELPLIFTELDAD
PEVRAVVLAAAGRHFSYGLDLPAMAGTFMPLMAEKALAKPRTDFLDEIRRLQASVTAVADCRKPVIAAIQGWCIGGGVDL
IAAADIRYASSEAKFSVREAKVAIVADIGSLHRLPPIIGDGHLRELAFTGKDIDAARAEKIGLVNDVFDTPEATLAAAHA
TAAEIAANPPLVVQGVKDVLGRERENVVADGLKYVSTWNAAFLPSEDLQEAIGAVFEKREPDFKGR

Sequences:

>Translated_306_residues
MADSLHASGSSVSDSLRASGSSVSDSLHASGSSADGWKAFTVETTGHIAQVTLIGPGKGNAMGPDFWRELPLIFTELDAD
PEVRAVVLAAAGRHFSYGLDLPAMAGTFMPLMAEKALAKPRTDFLDEIRRLQASVTAVADCRKPVIAAIQGWCIGGGVDL
IAAADIRYASSEAKFSVREAKVAIVADIGSLHRLPPIIGDGHLRELAFTGKDIDAARAEKIGLVNDVFDTPEATLAAAHA
TAAEIAANPPLVVQGVKDVLGRERENVVADGLKYVSTWNAAFLPSEDLQEAIGAVFEKREPDFKGR
>Mature_305_residues
ADSLHASGSSVSDSLRASGSSVSDSLHASGSSADGWKAFTVETTGHIAQVTLIGPGKGNAMGPDFWRELPLIFTELDADP
EVRAVVLAAAGRHFSYGLDLPAMAGTFMPLMAEKALAKPRTDFLDEIRRLQASVTAVADCRKPVIAAIQGWCIGGGVDLI
AAADIRYASSEAKFSVREAKVAIVADIGSLHRLPPIIGDGHLRELAFTGKDIDAARAEKIGLVNDVFDTPEATLAAAHAT
AAEIAANPPLVVQGVKDVLGRERENVVADGLKYVSTWNAAFLPSEDLQEAIGAVFEKREPDFKGR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=294, Percent_Identity=40.1360544217687, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=28.90625, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=26.5217391304348, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=26.5217391304348, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4502327, Length=250, Percent_Identity=29.2, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787659, Length=271, Percent_Identity=30.6273062730627, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI221142681, Length=267, Percent_Identity=31.8352059925094, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87082183, Length=279, Percent_Identity=27.9569892473118, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788682, Length=223, Percent_Identity=31.390134529148, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788597, Length=266, Percent_Identity=27.4436090225564, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=26.1538461538462, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790281, Length=181, Percent_Identity=27.6243093922652, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=268, Percent_Identity=34.3283582089552, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17534483, Length=270, Percent_Identity=33.7037037037037, Blast_Score=170, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17554946, Length=290, Percent_Identity=27.2413793103448, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=268, Percent_Identity=26.4925373134328, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=208, Percent_Identity=31.7307692307692, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=206, Percent_Identity=30.5825242718447, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558304, Length=191, Percent_Identity=27.2251308900524, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19920382, Length=252, Percent_Identity=37.6984126984127, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI20129971, Length=280, Percent_Identity=28.9285714285714, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24653477, Length=280, Percent_Identity=28.9285714285714, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24653139, Length=289, Percent_Identity=29.0657439446367, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24654903, Length=205, Percent_Identity=24.8780487804878, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24650670, Length=248, Percent_Identity=27.0161290322581, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 32243; Mature: 32112

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSLHASGSSVSDSLRASGSSVSDSLHASGSSADGWKAFTVETTGHIAQVTLIGPGKGN
CCCCCCCCCCCHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEECCCEEEEEEEECCCCC
AMGPDFWRELPLIFTELDADPEVRAVVLAAAGRHFSYGLDLPAMAGTFMPLMAEKALAKP
CCCHHHHHHHCCEEEECCCCCCCEEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHCCC
RTDFLDEIRRLQASVTAVADCRKPVIAAIQGWCIGGGVDLIAAADIRYASSEAKFSVREA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEECCEEECCCCCCEEHHHH
KVAIVADIGSLHRLPPIIGDGHLRELAFTGKDIDAARAEKIGLVNDVFDTPEATLAAAHA
EEEEEECCHHHHHCCCCCCCCCHHHHEECCCCCCHHHHHHCCCHHHHHCCCCHHHHHHHH
TAAEIAANPPLVVQGVKDVLGRERENVVADGLKYVSTWNAAFLPSEDLQEAIGAVFEKRE
HHHHHHCCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
PDFKGR
CCCCCC
>Mature Secondary Structure 
ADSLHASGSSVSDSLRASGSSVSDSLHASGSSADGWKAFTVETTGHIAQVTLIGPGKGN
CCCCCCCCCCHHHHHHCCCCCCCHHHHCCCCCCCCEEEEEEEECCCEEEEEEEECCCCC
AMGPDFWRELPLIFTELDADPEVRAVVLAAAGRHFSYGLDLPAMAGTFMPLMAEKALAKP
CCCHHHHHHHCCEEEECCCCCCCEEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHHCCC
RTDFLDEIRRLQASVTAVADCRKPVIAAIQGWCIGGGVDLIAAADIRYASSEAKFSVREA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCEEEEECCEEECCCCCCEEHHHH
KVAIVADIGSLHRLPPIIGDGHLRELAFTGKDIDAARAEKIGLVNDVFDTPEATLAAAHA
EEEEEECCHHHHHCCCCCCCCCHHHHEECCCCCCHHHHHHCCCHHHHHCCCCHHHHHHHH
TAAEIAANPPLVVQGVKDVLGRERENVVADGLKYVSTWNAAFLPSEDLQEAIGAVFEKRE
HHHHHHCCCCEEEHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
PDFKGR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA