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The map label for this gene is gatA

Identifier: 169630422

GI number: 169630422

Start: 3388186

End: 3389664

Strand: Reverse

Name: gatA

Synonym: MAB_3341c

Alternate gene names: 169630422

Gene position: 3389664-3388186 (Counterclockwise)

Preceding gene: 169630423

Following gene: 169630419

Centisome position: 66.89

GC content: 67.21

Gene sequence:

>1479_bases
GTGACCGACCTGATCAGGCAGGACGCCGCGGCGCTGGCCGCACTGATCCATTCGGGCGAGGTCTCCTCCGTCGAGGTGAC
ACGTGCGCATCTGGATCAGATCGCCAGCACCGACGAGACGTACCGGGCCTTCCTGCATGTTGCGGGGGAGCAGGCGCTGG
CCGCCGCGAAAGACGTCGATGACGCCATCGCGGCGGGCTCGGTGCCGTCGGGACTGGCCGGTGTCCCGCTGGCGCTCAAG
GACGTCTTCACCACTCGGGACATGCCGACCACCTGTGGTTCCAAGATCCTTGAGAACTGGGTGCCGCCCTACGACGCAAC
CGTGACGGCGCGGCTGCGCGGGGCCGGGATACCGATCCTGGGTAAGACGAACATGGACGAGTTCGCGATGGGCTCTTCCA
CCGAGAACTCCGCATACGGACCCACCCGCAATCCCTGGGACGTTGAGCGGGTTCCGGGCGGTTCGGGCGGTGGTAGCGCG
GCCGCGTTGGCCGCTTTCCAGGCTCCGCTGGCCATAGGCACCGACACCGGTGGCTCCATCCGGCAGCCCGCCGCACTCAC
CGCGACCGTCGGGGTGAAGCCCACCTATGGAACAGTTTCGCGTTTCGGCCTGGTCGCCTGTGCGTCATCGCTGGATCAGG
GCGGTCCTTGCGCGCGCACGGTTTTGGACACCGCGCTCTTGCACCAGGTGATCGCGGGCCACGATCCGCGCGACTCCACG
TCCGTCGACGAACCGGTACCCGATGTCGTGGCCGCGGCTCGCGCCGGTGCCTCGGGAGACCTCAAGGGTGTCCGCGTCGG
TGTGGTCACTCAGCTGCGTGGCGACGGGTACCAACCGGGTGTGATGGCGTCGTTCGACGCGGCCGTCGAGCAGCTCACCG
CATTGGGCGCCGATGTGGTTGAGGTGTCCTGTCCGCACTTCGAATACGCGTTGCCGGCCTATTACTTGATCCTGCCGTCG
GAGGTGTCGTCCAATCTGGCGCGCTTCGACGCCATGCGGTACGGCATGCGGGTCGGGGACGACGGAACGCATAGCGCCGA
GGAAGTCATGGCGCTCACCCGGGCGGCAGGCTTTGGACCAGAGGTCAAGCGCCGCATCATGATCGGCACCTATGCACTGT
CCGCCGGGTACTACGACGCCTACTACGGCCGGGCACAGAAGGTGCGCACCCTGATCAAGCGCGATCTGGAGCGGGCCTAC
GAGCAGGTCGACGTGCTGGTCACCCCGGCCACCCCCACCACCGCATTCCGGTTGGGGGAGAAGGTCGATGACCCGCTGGC
GATGTACCAGTTCGACTTGTGCACCCTGCCGCTGAACCTGGCCGGACACTGCGGAATGTCGGTGCCCTCAGGTCTTTCTG
CCGACGACGGGTTGCCGGTGGGTCTGCAGATCATGGCCCCCGCGCTCGCCGACGACCGGTTGTACCGCGTGGGTGCTGCC
TATGAAACCGCCCGTGGGGCGTTGCCCGCAGCGCTGTAG

Upstream 100 bases:

>100_bases
ATGTGATCGCCGAATGCCTTACCCCGGAGGAGGCGCTGGCCGCGGCGCCCCGGGTGGCCGAGGGACGTTTCGCGGTGCCG
CAGATTTTGGGGGAAGAAGA

Downstream 100 bases:

>100_bases
GAACGCACAGGTAACTGGCAGTTGATAAGGGGCGATTCCCGGCCGTCTGCTGCGTACCGTGAAGTGCATGCCCCGCCTAT
CGTCGTGCGTCGCGACCATC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MTDLIRQDAAALAALIHSGEVSSVEVTRAHLDQIASTDETYRAFLHVAGEQALAAAKDVDDAIAAGSVPSGLAGVPLALK
DVFTTRDMPTTCGSKILENWVPPYDATVTARLRGAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWDVERVPGGSGGGSA
AALAAFQAPLAIGTDTGGSIRQPAALTATVGVKPTYGTVSRFGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDST
SVDEPVPDVVAAARAGASGDLKGVRVGVVTQLRGDGYQPGVMASFDAAVEQLTALGADVVEVSCPHFEYALPAYYLILPS
EVSSNLARFDAMRYGMRVGDDGTHSAEEVMALTRAAGFGPEVKRRIMIGTYALSAGYYDAYYGRAQKVRTLIKRDLERAY
EQVDVLVTPATPTTAFRLGEKVDDPLAMYQFDLCTLPLNLAGHCGMSVPSGLSADDGLPVGLQIMAPALADDRLYRVGAA
YETARGALPAAL

Sequences:

>Translated_492_residues
MTDLIRQDAAALAALIHSGEVSSVEVTRAHLDQIASTDETYRAFLHVAGEQALAAAKDVDDAIAAGSVPSGLAGVPLALK
DVFTTRDMPTTCGSKILENWVPPYDATVTARLRGAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWDVERVPGGSGGGSA
AALAAFQAPLAIGTDTGGSIRQPAALTATVGVKPTYGTVSRFGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDST
SVDEPVPDVVAAARAGASGDLKGVRVGVVTQLRGDGYQPGVMASFDAAVEQLTALGADVVEVSCPHFEYALPAYYLILPS
EVSSNLARFDAMRYGMRVGDDGTHSAEEVMALTRAAGFGPEVKRRIMIGTYALSAGYYDAYYGRAQKVRTLIKRDLERAY
EQVDVLVTPATPTTAFRLGEKVDDPLAMYQFDLCTLPLNLAGHCGMSVPSGLSADDGLPVGLQIMAPALADDRLYRVGAA
YETARGALPAAL
>Mature_491_residues
TDLIRQDAAALAALIHSGEVSSVEVTRAHLDQIASTDETYRAFLHVAGEQALAAAKDVDDAIAAGSVPSGLAGVPLALKD
VFTTRDMPTTCGSKILENWVPPYDATVTARLRGAGIPILGKTNMDEFAMGSSTENSAYGPTRNPWDVERVPGGSGGGSAA
ALAAFQAPLAIGTDTGGSIRQPAALTATVGVKPTYGTVSRFGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDSTS
VDEPVPDVVAAARAGASGDLKGVRVGVVTQLRGDGYQPGVMASFDAAVEQLTALGADVVEVSCPHFEYALPAYYLILPSE
VSSNLARFDAMRYGMRVGDDGTHSAEEVMALTRAAGFGPEVKRRIMIGTYALSAGYYDAYYGRAQKVRTLIKRDLERAYE
QVDVLVTPATPTTAFRLGEKVDDPLAMYQFDLCTLPLNLAGHCGMSVPSGLSADDGLPVGLQIMAPALADDRLYRVGAAY
ETARGALPAAL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=496, Percent_Identity=37.0967741935484, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI195972892, Length=503, Percent_Identity=26.0437375745527, Blast_Score=132, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17543272, Length=416, Percent_Identity=37.5, Blast_Score=250, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17537465, Length=512, Percent_Identity=25.9765625, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=304, Percent_Identity=28.2894736842105, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=188, Percent_Identity=30.8510638297872, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=190, Percent_Identity=29.4736842105263, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=324, Percent_Identity=26.8518518518519, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=198, Percent_Identity=31.3131313131313, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=414, Percent_Identity=33.0917874396135, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319685, Length=427, Percent_Identity=31.1475409836066, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=39.7959183673469, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24644968, Length=518, Percent_Identity=26.6409266409266, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=25.2032520325203, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=25.2032520325203, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=25.2032520325203, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=25.2032520325203, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI45550774, Length=206, Percent_Identity=33.0097087378641, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648435, Length=206, Percent_Identity=33.0097087378641, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648437, Length=206, Percent_Identity=33.0097087378641, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=25.4, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356731, Length=252, Percent_Identity=26.984126984127, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648441, Length=181, Percent_Identity=31.4917127071823, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648439, Length=181, Percent_Identity=31.4917127071823, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_MYCA9 (B1MDU8)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001704071.1
- ProteinModelPortal:   B1MDU8
- SMR:   B1MDU8
- EnsemblBacteria:   EBMYCT00000005414
- GeneID:   5965841
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3341c
- GeneTree:   EBGT00050000014631
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   MABS561007:MAB_3341C-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51323; Mature: 51192

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 80-80 ACT_SITE 155-155 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLIRQDAAALAALIHSGEVSSVEVTRAHLDQIASTDETYRAFLHVAGEQALAAAKDVD
CCHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
DAIAAGSVPSGLAGVPLALKDVFTTRDMPTTCGSKILENWVPPYDATVTARLRGAGIPIL
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEE
GKTNMDEFAMGSSTENSAYGPTRNPWDVERVPGGSGGGSAAALAAFQAPLAIGTDTGGSI
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCC
RQPAALTATVGVKPTYGTVSRFGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDST
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
SVDEPVPDVVAAARAGASGDLKGVRVGVVTQLRGDGYQPGVMASFDAAVEQLTALGADVV
CCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCE
EVSCPHFEYALPAYYLILPSEVSSNLARFDAMRYGMRVGDDGTHSAEEVMALTRAAGFGP
EECCCCCHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCH
EVKRRIMIGTYALSAGYYDAYYGRAQKVRTLIKRDLERAYEQVDVLVTPATPTTAFRLGE
HHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHCC
KVDDPLAMYQFDLCTLPLNLAGHCGMSVPSGLSADDGLPVGLQIMAPALADDRLYRVGAA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCCEEECCCH
YETARGALPAAL
HHHHCCCCCCCC
>Mature Secondary Structure 
TDLIRQDAAALAALIHSGEVSSVEVTRAHLDQIASTDETYRAFLHVAGEQALAAAKDVD
CHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
DAIAAGSVPSGLAGVPLALKDVFTTRDMPTTCGSKILENWVPPYDATVTARLRGAGIPIL
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCEE
GKTNMDEFAMGSSTENSAYGPTRNPWDVERVPGGSGGGSAAALAAFQAPLAIGTDTGGSI
CCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEECCCCCCC
RQPAALTATVGVKPTYGTVSRFGLVACASSLDQGGPCARTVLDTALLHQVIAGHDPRDST
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
SVDEPVPDVVAAARAGASGDLKGVRVGVVTQLRGDGYQPGVMASFDAAVEQLTALGADVV
CCCCCCHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCE
EVSCPHFEYALPAYYLILPSEVSSNLARFDAMRYGMRVGDDGTHSAEEVMALTRAAGFGP
EECCCCCHHCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCH
EVKRRIMIGTYALSAGYYDAYYGRAQKVRTLIKRDLERAYEQVDVLVTPATPTTAFRLGE
HHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHCC
KVDDPLAMYQFDLCTLPLNLAGHCGMSVPSGLSADDGLPVGLQIMAPALADDRLYRVGAA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHCCCCEEECCCH
YETARGALPAAL
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA