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The map label for this gene is leuD

Identifier: 169630374

GI number: 169630374

Start: 3332079

End: 3332678

Strand: Reverse

Name: leuD

Synonym: MAB_3293c

Alternate gene names: 169630374

Gene position: 3332678-3332079 (Counterclockwise)

Preceding gene: 169630375

Following gene: 169630373

Centisome position: 65.77

GC content: 59.33

Gene sequence:

>600_bases
GTGGAGGCATTCGACACCCACACCGGAATTGGTGTGCCGCTGCGCCGTTCCAACGTGGACACCGACCAGATCATCCCGGC
GGTCTACCTCAAGCGTGTCACCCGAACGGGTTTCGAGGACGGGCTCTTCGCCGGTTGGCGTACCGACCCCTCCTTCATTC
TCAATCTGGAACCGTTCAACCGGGGCACTGTTCTGGTCGCGGGGCCGGAATTCGGCACCGGATCGTCCCGAGAGCACGCT
GTGTGGGCCTTGATGGACTATGGATTTCGAGTAGTACTGTCGTCCCGTTTCGGAGATATCTTCCGGGGCAATGCGGGTAA
GGCCGGTCTGGTCGCCGGATTGGTGGGTCAGTCCGACATCGAGCTGCTCTGGAAGCTCATCGAGCAGAATCCGGGTTTGG
AACTCACTGTGAATCTTGAGGATCGGACGGTGACCGCTGGAACGGCGGTGGTGCCGTTTGAGATTGACGACTACACGCGG
TGGCGTCTGCTCGAAGGATTGGACGATATAGGCCTTACGCTGCGAAAAGTCGATGAAATTGCGGCTTTTGAGGCCGCTCG
GCCGGAGTTCAAACCCCGTACTTTGGAGCCATCCGAGTAG

Upstream 100 bases:

>100_bases
CCGTCGCCGCGGCCACGGCGGTGAGGGGAAAGCTCTCATCGCCAGCTGATTTGGCAAAATCCGTAAAATAGGCACCTGCC
AAAAGGCACAGGAGGAAATA

Downstream 100 bases:

>100_bases
CTGATTGGGCGCCCTTGGGGGGCAAAGGCATTCCTTTTGGTCGGCGAATGGCTCCGTAAAGAGCTCAGAGAATTTTGCCG
AAAATACGCGTGGCACTTGG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MEAFDTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAGWRTDPSFILNLEPFNRGTVLVAGPEFGTGSSREHA
VWALMDYGFRVVLSSRFGDIFRGNAGKAGLVAGLVGQSDIELLWKLIEQNPGLELTVNLEDRTVTAGTAVVPFEIDDYTR
WRLLEGLDDIGLTLRKVDEIAAFEAARPEFKPRTLEPSE

Sequences:

>Translated_199_residues
MEAFDTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAGWRTDPSFILNLEPFNRGTVLVAGPEFGTGSSREHA
VWALMDYGFRVVLSSRFGDIFRGNAGKAGLVAGLVGQSDIELLWKLIEQNPGLELTVNLEDRTVTAGTAVVPFEIDDYTR
WRLLEGLDDIGLTLRKVDEIAAFEAARPEFKPRTLEPSE
>Mature_199_residues
MEAFDTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAGWRTDPSFILNLEPFNRGTVLVAGPEFGTGSSREHA
VWALMDYGFRVVLSSRFGDIFRGNAGKAGLVAGLVGQSDIELLWKLIEQNPGLELTVNLEDRTVTAGTAVVPFEIDDYTR
WRLLEGLDDIGLTLRKVDEIAAFEAARPEFKPRTLEPSE

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=197, Percent_Identity=45.1776649746193, Blast_Score=176, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6321429, Length=203, Percent_Identity=41.871921182266, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_MYCA9 (B1MDQ0)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001704023.1
- ProteinModelPortal:   B1MDQ0
- SMR:   B1MDQ0
- EnsemblBacteria:   EBMYCT00000004670
- GeneID:   5965793
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3293c
- GeneTree:   EBGT00050000015493
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   MABS561007:MAB_3293C-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22040; Mature: 22040

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAFDTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAGWRTDPSFILNLEPFN
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCC
RGTVLVAGPEFGTGSSREHAVWALMDYGFRVVLSSRFGDIFRGNAGKAGLVAGLVGQSDI
CCEEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHCCCCCCCCEEEEECCCHHH
ELLWKLIEQNPGLELTVNLEDRTVTAGTAVVPFEIDDYTRWRLLEGLDDIGLTLRKVDEI
HHHHHHHHCCCCCEEEEEECCCEEECCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHH
AAFEAARPEFKPRTLEPSE
HHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEAFDTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAGWRTDPSFILNLEPFN
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEECCCC
RGTVLVAGPEFGTGSSREHAVWALMDYGFRVVLSSRFGDIFRGNAGKAGLVAGLVGQSDI
CCEEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHCCCCCCCCEEEEECCCHHH
ELLWKLIEQNPGLELTVNLEDRTVTAGTAVVPFEIDDYTRWRLLEGLDDIGLTLRKVDEI
HHHHHHHHCCCCCEEEEEECCCEEECCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHH
AAFEAARPEFKPRTLEPSE
HHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA